rabbit polyclonal
reactivity: human, rat
modification: phosphorylated
application: western blot
reactivity: human, rat
modification: phosphorylated
application: western blot
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, enzyme immunoassay
reactivity: human, mouse, rat
application: western blot, ELISA, enzyme immunoassay
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
rabbit polyclonal
reactivity: human, mouse
application: western blot, immunoprecipitation
reactivity: human, mouse
application: western blot, immunoprecipitation
Western blot analysis of extracts of HeLa cells, using Phospho-RAF1-S259 antibody (MBS9128987) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-RAF1-S259 pAb (MBS9128987). Western blot was performed from the immunoprecipitate using Phospho- RAF1-S259 pAb (MBS9128987) at a dilition of 1:1000. HeLa cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.
quantity: 0.05 mL
price: 235 USD
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