rabbit polyclonal
reactivity: human, mouse, rat, dog, chicken, cow
application: western blot
reactivity: human, mouse, rat, dog, chicken, cow
application: western blot
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
rabbit polyclonal
reactivity: human, mouse, rat, dog
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat, dog
application: western blot, immunohistochemistry, immunocytochemistry


Western blot analysis of PPAR gamma expression in HeLa (A), MCF7 (B), mouse brain (C) whole cell lysates.

Immunohistochemical analysis of PPAR gamma staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PPAR gamma staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price: 155 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, enzyme immunoassay
reactivity: human, mouse, rat
application: western blot, ELISA, enzyme immunoassay