mouse monoclonal (10T1)
reactivity: human
application: western blot, ELISA, immunoprecipitation, enzyme immunoassay
reactivity: human
application: western blot, ELISA, immunoprecipitation, enzyme immunoassay
mouse monoclonal (P10T1AT)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay
mouse monoclonal (10T1)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay
rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS
Western blot analysis of IRF5 expression in THP-1 cell lysate (MBS179503). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF5
quantity: 0.03 mg
price: 160 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
