rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
mouse monoclonal (6-May)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay
rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section
MBS9207040 staining AMH in human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Overlay histogram showing SH-SY5Y cells stained with AP9940c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9207040, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-AMH Antibody (Center) at 1:2000 dilution . Lane 1: 293 whole cell lysate . Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
quantity: 0.08 mL
price: 165 USD
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