rabbit polyclonal
reactivity: human, rat, dog, cow
application: immunohistochemistry, immunohistochemistry - paraffin section
reactivity: human, rat, dog, cow
application: immunohistochemistry, immunohistochemistry - paraffin section
rabbit polyclonal
reactivity: mouse, rat
application: western blot
reactivity: mouse, rat
application: western blot
rabbit monoclonal ([DCB-3])
reactivity: human, rat
application: western blot, immunohistochemistry
reactivity: human, rat
application: western blot, immunohistochemistry
Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-1 Antibody (MBS179060) CASP1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP1 Antibody (MBS179060) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (MBS176451) with DAB as the chromogen.
Western blot analysis of Caspase-1 in HeLa cell lysate (MBS179060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 monoclonal antibody (MBS179060) overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) (MBS176460) kit with Tanon 5200 system. A specific band was detected for CASP1.
quantity: 0.03 mg
price: 160 USD
to the supplier
unidentified polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
Western blot analysis of Caspase 1 p10 expression in HeLa (A), mouse spleen (B), rat spleen (C) whole cell lysates.
Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity: 0.03 mL
price: 155 USD
to the supplier