rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
rabbit polyclonal
reactivity: human, mouse, rat, dog, cow
application: immunohistochemistry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat, dog, cow
application: immunohistochemistry, immunohistochemistry - paraffin section
unidentified polyclonal
reactivity: human, mouse, rat, cow
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat, cow
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of FAP alpha expression in Hela (A), MCF7 (B), NIH3T3 (C), mouse kidney (D), rat liver (E) whole cell lysates.
Immunohistochemical analysis of FAP alpha staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FAP alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity: 0.03 mL
price: 155 USD
to the supplier