goat polyclonal
reactivity: human
application: western blot, enzyme immunoassay
reactivity: human
application: western blot, enzyme immunoassay
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of extracts of various cell lines, using F2R antibody (MBS9127538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
quantity: 0.05 mL
price: 200 USD
to the supplier
unidentified polyclonal
reactivity: human
application: western blot, immunocytochemistry
reactivity: human
application: western blot, immunocytochemistry
Western blot analysis of PAR1 expression in PaCa2 (A), HeLa (B), TF1 (C), ECV304 (D) whole cell lysates.
Immunofluorescent analysis of PAR1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity: 0.03 mL
price: 155 USD
to the supplier