Rabbit anti Phospho- IRS-1 (pSer 312) antibody
MyBioSource
catalog: MBS462057

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry

quantity: 0.1 mg
price: 350 USD
to the supplier

Rabbit Polyclonal to Human IRS1 / IRS-1
MyBioSource
catalog: MBS241289

rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 0.05 mg
price: 495 USD
to the supplier

IRS-1 Antibody
MyBioSource
catalog: MBS150253

rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, enzyme immunoassay

quantity: 0.1 mg
price: 345 USD
to the supplier

IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)
MyBioSource
catalog: MBS624304

rabbit polyclonal
reactivity: human, mouse

Western Blot using MBS624304.

Immunohistochemistry analysis of Phospho-IRS1 [pY612] showing staining in the cytoplasm and nucleus of paraffin-embedded human skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at RT, washed with ddH2O and PBS, and then probed with MBS624304 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4 C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Flow cytometry analysis of IRS1 [pY612] was done on MCF7 cells treated with Insulin (100nM, 10 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at RT. Cells were labeled with MBS624304 (red histogram) or with rabbit isotype control (pink histogram) at 3-5ug/million cells in 2.5% BSA. After incubation at RT for 2 hours, the cells were labeled with Alexa Fluor 488 Goat Anti-Rabbit Secondary Antibody at a dilution of 1:400 for 30 minutes at RT. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
quantity: 0.1 mL
price: 635 USD
to the supplier

Phospho-IRS1-S307 Polyclonal Antibody
MyBioSource
catalog: MBS9128837

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of extracts of mouse brain, using Phospho-IRS1-S307 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
quantity: 0.05 mL
price: 235 USD
to the supplier

Phospho-IRS1-S307 Polyclonal Antibody
MyBioSource
catalog: MBS128544

rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-IRS1-S307 antibody.
quantity: 0.05 mL
price: 235 USD
to the supplier

Phospho-IRS1-S636 Polyclonal Antibody
MyBioSource
catalog: MBS128253

unidentified polyclonal
reactivity: human, mouse
application: western blot

Western blot analysis of extracts from 3T3 cells, using Phospho-IRS1-S636 antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
quantity: 0.05 mL
price: 235 USD
to the supplier