rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, enzyme immunoassay
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, enzyme immunoassay
rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry


Western blot analysis of Collagen 3 alpha 1 expression in A10 (A), HeLa (B), mouse lung (C), mouse liver (D), rat lung (E) whole cell lysates.

Immunohistochemical analysis of Collagen 3 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Collagen 3 alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity: 0.03 mL
price: 155 USD
to the supplier
mouse monoclonal ([A6])
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation


Western Blot; Sample: Human Hela cell lysate; Primary Ab: 6ug/ml Mouse Anti-Multi-species PIIINP Antibody

Western Blot; Sample: Human HepG2 cell lysate;. Primary Ab: 5ug/ml Mouse Anti-Multi-species PIIINP Antibody. Secondary Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (MBS2086035)

Figure. DAB staining on IHC-P; Samples: Human Stomach Tissue.
quantity: 0.01 mg
price: 130 USD
to the supplier