rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, enzyme immunoassay
reactivity: human, mouse, rat
application: western blot, ELISA, enzyme immunoassay
rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
Anti-CMA1 Picoband antibody, MBS176889. IHC(P): Human Placenta Tissue
Western blot analysis of CMA1 using anti- CMA1 antibody (MBS176889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates,. Lane 2: human HepG2 whole cell lysates,. Lane 3: rat liver tissue lysates,. Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CMA1 antigen affinity purified polyclonal antibody (Catalog # MBS176889) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CMA1 at approximately 35KD. The expected band size for CMA1 is at 27KD.
quantity: 0.1 mg
price: 315 USD
to the supplier
mouse monoclonal ([2Q1505])
reactivity: human
application: immunohistochemistry, other
reactivity: human
application: immunohistochemistry, other
High magnification: Paraffin-embedded human skin tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of MBS600099. Cells lining up under the epidermis consist of numerous mast cells (The dermis has a moderate diffuse inflammatory cell infiltrate).
quantity: 0.1 mg
price: 630 USD
to the supplier
sheep polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation, enzyme immunoassay
reactivity: human
application: western blot, ELISA, immunoprecipitation, enzyme immunoassay
