Active human PAI-1 functional assay ELISA kit | Molecular Innovations HPAIKT product information

product summary

company name :

Molecular Innovations

product type :

ELISA/assay

product name :

Active human PAI-1 functional assay ELISA kit

catalog :

HPAIKT

quantity :

96 Tests

price :

475 USD

more info or order :

Molecular Innovations product webpage

citations: 3

Reference
Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. J Biol Chem. 2012;287:26478-94 pubmed publisher
Richardson M, Gupta A, O'Brien L, Berg D, Gerlitz B, Syed S, et al. Treatment of sepsis-induced acquired protein C deficiency reverses Angiotensin-converting enzyme-2 inhibition and decreases pulmonary inflammatory response. J Pharmacol Exp Ther. 2008;325:17-26 pubmed publisher
Wu J, Peng L, McMahon G, Lawrence D, Fay W. Recombinant plasminogen activator inhibitor-1 inhibits intimal hyperplasia. Arterioscler Thromb Vasc Biol. 2009;29:1565-70 pubmed publisher

product information

Catalog Number :

HPAIKT

Product Name :

Active human PAI-1 functional assay ELISA kit

Product Type :

ELISA

Size :

96 Tests

List Price :

475 USD

Gene ID :

P05121

Product Rank :

Product Description :

The sensitive quantitative measurement of functionally active human PAI-1 in plasma samples is easily performed with this 96 well strip format ELISA kit. The PAI-1 concentration of normal platelet-free plasma is 21 ng/ml, platelet-rich plasma is 283 ng/ml and serum is 270 ng/ml. 1 PAI-1 unit = 1.34 ng. The assay measures active PAI-1 in the 0.125-100 U/ml range. Samples giving human PAI-1 levels above 100 U/ml should be diluted in PAI-1 depleted plasma before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. Validated in citrate, EDTA, and heparin plasma. Vitronectin does not interfere with the detection of active PAI-1. This kit does not cross react with mouse PAI-1. After appropriate washing steps, anti human PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared in PAI-1 depleted plasma using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits. The Human PAI-1 standard provided in this kit is calibrated against the WHO International Standard for Human PAI-1 (NIBSC Code 92/654).

CrossReactivity :

Does not cross react with mouse PAI-1.

SpeciesSummary :

Human

Background :

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.

ALTnames :

SERPINE1

Specificity :

Does not cross react with mouse PAI-1.

Uses :

Plasma, serum, cell culture media

Storage :

4 C

more info or order :

Molecular Innovations product webpage