product summary
Loading...
company name :
Miltenyi Biotec
product type :
antibody
product name :
CD303 (BDCA-2) Antibody, anti-human, pure
catalog :
130-090-690
quantity :
100 µg in 1 mL
price :
160 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AC144
reactivity :
African green monkey, human
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 45
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:2; loading ...; fig 1b
Chen Y, Gomes T, Hardman C, Vieira Braga F, Gutowska Owsiak D, Salimi M, et al. Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; loading ...; tbl s1
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Delso Vallejo M, Kollet J, Koehl U, Huppert V. Influence of Irradiated Peripheral Blood Mononuclear Cells on Both Ex Vivo Proliferation of Human Natural Killer Cells and Change in Cellular Property. Front Immunol. 2017;8:854 pubmed publisher
  • flow cytometry; human; fig 2b
See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
  • flow cytometry; human; loading ...; fig st12
Villani A, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J, et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science. 2017;356: pubmed publisher
  • flow cytometry; human; loading ...; fig 1d
Cottineau J, Kottemann M, Lach F, Kang Y, Vély F, Deenick E, et al. Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. J Clin Invest. 2017;127:1991-2006 pubmed publisher
  • flow cytometry; human; loading ...; fig 1d
Schuh E, Musumeci A, Thaler F, Laurent S, Ellwart J, Hohlfeld R, et al. Human Plasmacytoid Dendritic Cells Display and Shed B Cell Maturation Antigen upon TLR Engagement. J Immunol. 2017;198:3081-3088 pubmed publisher
  • flow cytometry; human; loading ...; fig 4c
Sontag S, Förster M, Qin J, Wanek P, Mitzka S, Schüler H, et al. Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells. Stem Cells. 2017;35:898-908 pubmed publisher
  • immunohistochemistry; human; 1:50; loading ...; fig e-4
Uruha A, Nishikawa A, Tsuburaya R, Hamanaka K, Kuwana M, Watanabe Y, et al. Sarcoplasmic MxA expression: A valuable marker of dermatomyositis. Neurology. 2017;88:493-500 pubmed publisher
  • flow cytometry; human; loading ...
Mascarell L, Airouche S, Berjont N, Gary C, Gueguen C, Fourcade G, et al. The regulatory dendritic cell marker C1q is a potent inhibitor of allergic inflammation. Mucosal Immunol. 2017;10:695-704 pubmed publisher
  • flow cytometry; human; 1:10; fig s2c
Reinisch A, Thomas D, Corces M, Zhang X, Gratzinger D, Hong W, et al. A humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells. Nat Med. 2016;22:812-21 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Schulz A, Mälzer J, Domingo C, Jürchott K, Grützkau A, Babel N, et al. Low Thymic Activity and Dendritic Cell Numbers Are Associated with the Immune Response to Primary Viral Infection in Elderly Humans. J Immunol. 2015;195:4699-711 pubmed publisher
  • flow cytometry; human; fig 5
Anandasabapathy N, Breton G, Hurley A, Caskey M, Trumpfheller C, Sarma P, et al. Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers. Bone Marrow Transplant. 2015;50:924-30 pubmed publisher
  • immunocytochemistry; human
Yu C, Becker C, Metang P, Marches F, Wang Y, Toshiyuki H, et al. Human CD141+ dendritic cells induce CD4+ T cells to produce type 2 cytokines. J Immunol. 2014;193:4335-43 pubmed publisher
  • flow cytometry; human
Balan S, Ollion V, Colletti N, Chelbi R, Montanana Sanchis F, Liu H, et al. Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells. J Immunol. 2014;193:1622-35 pubmed publisher
  • flow cytometry; African green monkey; fig 2
Jacquelin B, Petitjean G, Kunkel D, Liovat A, Jochems S, Rogers K, et al. Innate immune responses and rapid control of inflammation in African green monkeys treated or not with interferon-alpha during primary SIVagm infection. PLoS Pathog. 2014;10:e1004241 pubmed publisher
  • immunohistochemistry - frozen section; human
Vittorakis S, Samitas K, Tousa S, Zervas E, Aggelakopoulou M, Semitekolou M, et al. Circulating conventional and plasmacytoid dendritic cell subsets display distinct kinetics during in vivo repeated allergen skin challenges in atopic subjects. Biomed Res Int. 2014;2014:231036 pubmed publisher
Hjorton K, Hagberg N, Israelsson E, Jinton L, Berggren O, Sandling J, et al. Cytokine production by activated plasmacytoid dendritic cells and natural killer cells is suppressed by an IRAK4 inhibitor. Arthritis Res Ther. 2018;20:238 pubmed publisher
Sauss K, Ehrentraut S, Zenclussen A, Schumacher A. The pregnancy hormone human chorionic gonadotropin differentially regulates plasmacytoid and myeloid blood dendritic cell subsets. Am J Reprod Immunol. 2018;79:e12837 pubmed publisher
Jeffery H, McDowell P, Lutz P, Wawman R, Roberts S, Bagnall C, et al. Human intrahepatic ILC2 are IL-13positive amphiregulinpositive and their frequency correlates with model of end stage liver disease score. PLoS ONE. 2017;12:e0188649 pubmed publisher
Maric J, Ravindran A, Mazzurana L, Björklund Ã, Van Acker A, Rao A, et al. Prostaglandin E2 suppresses human group 2 innate lymphoid cell function. J Allergy Clin Immunol. 2018;141:1761-1773.e6 pubmed publisher
McDonnell A, Cook A, Robinson B, Lake R, Nowak A. Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters. BMC Cancer. 2017;17:417 pubmed publisher
Krupna Gaylord M, Liveris D, Love A, Wormser G, Schwartz I, Petzke M. Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36. PLoS ONE. 2014;9:e100174 pubmed publisher
Zhang Y, Shi W, Tang S, Li J, Yin S, Gao X, et al. The influence of cathelicidin LL37 in human anti-neutrophils cytoplasmic antibody (ANCA)-associated vasculitis. Arthritis Res Ther. 2013;15:R161 pubmed publisher
Lehmann C, Jung N, Förster K, Koch N, Leifeld L, Fischer J, et al. Longitudinal analysis of distribution and function of plasmacytoid dendritic cells in peripheral blood and gut mucosa of HIV infected patients. J Infect Dis. 2014;209:940-9 pubmed publisher
Vanders R, Gibson P, Murphy V, Wark P. Plasmacytoid dendritic cells and CD8 T cells from pregnant women show altered phenotype and function following H1N1/09 infection. J Infect Dis. 2013;208:1062-70 pubmed publisher
Chung N, Matthews K, Klasse P, Sanders R, Moore J. HIV-1 gp120 impairs the induction of B cell responses by TLR9-activated plasmacytoid dendritic cells. J Immunol. 2012;189:5257-65 pubmed publisher
Dutertre C, Amraoui S, Derosa A, Jourdain J, Vimeux L, Goguet M, et al. Pivotal role of M-DC8? monocytes from viremic HIV-infected patients in TNF? overproduction in response to microbial products. Blood. 2012;120:2259-68 pubmed publisher
Kuzin I, Sun H, Moshkani S, Feng C, Mantalaris A, Wu J, et al. Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor. Biotechnol Bioeng. 2011;108:1430-40 pubmed publisher
Contreras V, Urien C, Guiton R, Alexandre Y, Vu Manh T, Andrieu T, et al. Existence of CD8?-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species. J Immunol. 2010;185:3313-25 pubmed publisher
Stary G, Bangert C, Tauber M, Strohal R, Kopp T, Stingl G. Tumoricidal activity of TLR7/8-activated inflammatory dendritic cells. J Exp Med. 2007;204:1441-51 pubmed
Nestle F, Conrad C, Tun Kyi A, Homey B, Gombert M, Boyman O, et al. Plasmacytoid predendritic cells initiate psoriasis through interferon-alpha production. J Exp Med. 2005;202:135-43 pubmed
Ebner S, Ehammer Z, Holzmann S, Schwingshackl P, Forstner M, Stoitzner P, et al. Expression of C-type lectin receptors by subsets of dendritic cells in human skin. Int Immunol. 2004;16:877-87 pubmed
De Wit D, Olislagers V, Goriely S, Vermeulen F, Wagner H, Goldman M, et al. Blood plasmacytoid dendritic cell responses to CpG oligodeoxynucleotides are impaired in human newborns. Blood. 2004;103:1030-2 pubmed
Clark F, Freeman L, Dzionek A, Schmitz J, McMullan D, Simpson P, et al. Origin and subset distribution of peripheral blood dendritic cells in patients with chronic graft-versus-host disease. Transplantation. 2003;75:221-5 pubmed
Dzionek A, Inagaki Y, Okawa K, Nagafune J, Röck J, Sohma Y, et al. Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum Immunol. 2002;63:1133-48 pubmed
Wollenberg A, Wagner M, Günther S, Towarowski A, Tuma E, Moderer M, et al. Plasmacytoid dendritic cells: a new cutaneous dendritic cell subset with distinct role in inflammatory skin diseases. J Invest Dermatol. 2002;119:1096-102 pubmed
Arpinati M, Chirumbolo G, Urbini B, Martelli V, Stanzani M, Falcioni S, et al. Use of anti-BDCA-2 antibody for detection of dendritic cells type-2 (DC2) in allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant. 2002;29:887-91 pubmed
Ebner S, Hofer S, Nguyen V, Fürhapter C, Herold M, Fritsch P, et al. A novel role for IL-3: human monocytes cultured in the presence of IL-3 and IL-4 differentiate into dendritic cells that produce less IL-12 and shift Th cell responses toward a Th2 cytokine pattern. J Immunol. 2002;168:6199-207 pubmed
Chehimi J, Campbell D, Azzoni L, Bacheller D, Papasavvas E, Jerandi G, et al. Persistent decreases in blood plasmacytoid dendritic cell number and function despite effective highly active antiretroviral therapy and increased blood myeloid dendritic cells in HIV-infected individuals. J Immunol. 2002;168:4796-801 pubmed
Ronnblom L, Alm G. A pivotal role for the natural interferon alpha-producing cells (plasmacytoid dendritic cells) in the pathogenesis of lupus. J Exp Med. 2001;194:F59-63 pubmed
Dzionek A, Sohma Y, Nagafune J, Cella M, Colonna M, Facchetti F, et al. BDCA-2, a novel plasmacytoid dendritic cell-specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon alpha/beta induction. J Exp Med. 2001;194:1823-34 pubmed
Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S, et al. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol. 2000;165:6037-46 pubmed
Grabbe S, Kämpgen E, Schuler G. Dendritic cells: multi-lineal and multi-functional. Immunol Today. 2000;21:431-3 pubmed
product information
MB Cataloge Number :
130-090-690
Catalog Number :
130-090-690
Product Name :
CD303 (BDCA-2) Antibody, anti-human, pure
Product Type :
Primary antibodies
Host Species :
mouse IgG1κ
Clonality :
monoclonal
Conjugation :
pure
Antigen Modification :
CD303 (BDCA-2)
Clone Name :
AC144
Isotype :
Isotype Control Antibody, mouse IgG1
Size :
100 µg in 1 mL
US List Price :
160 USD
Product Description :
Detection of human plasmacytoid dendritic cells in blood, lymphoid, and non-lymphoid tissues
CrossReactivity :
cynomolgus monkey (Macaca fascicularis)
ALTnames :
CLEC4C, BDCA-2, CLECSF11, CLECSF7, DLEC, HECL, PRO34150
Specificity :
human, non-human primate
Uses :
For research use only
Application Summary :
Flow cytometry
Storage :
Store protected from light at 2–8 °C. Do not freeze.
more info or order :
company information
Miltenyi Biotec
Friedrich-Ebert-Strae 68
51429 Bergisch Gladbach
macs@miltenyibiotec.com
https://www.miltenyibiotec.com
1 800 FOR MACS
headquarters: Germany