product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-YAP1 antibody produced in mouse
catalog :
WH0010413M1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2F12
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation, immunocytochemistry knockout validation
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry knockout validation; mouse; 1:200; loading ...; fig 2a
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 3f
Huang Z, Hu J, Pan J, Wang Y, Hu G, Zhou J, et al. YAP stabilizes SMAD1 and promotes BMP2-induced neocortical astrocytic differentiation. Development. 2016;143:2398-409 pubmed publisher
  • immunoprecipitation; mouse; loading ...; fig 3e
Rojek K, Krzemien J, Dolezyczek H, Boguszewski P, Kaczmarek L, Konopka W, et al. Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. PLoS Biol. 2019;17:e3000253 pubmed publisher
  • immunohistochemistry; human; fig 6
  • immunohistochemistry; mouse; fig 4
Nishio M, Sugimachi K, Goto H, Wang J, Morikawa T, Miyachi Y, et al. Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A. 2016;113:E71-80 pubmed publisher
Xu X, Shen X, Feng W, Yang D, Jin L, Wang J, et al. D-galactose induces senescence of glioblastoma cells through YAP-CDK6 pathway. Aging (Albany NY). 2020;12:18501-18521 pubmed publisher
Wen T, Liu J, He X, Dong K, Hu G, Yu L, et al. Transcription factor TEAD1 is essential for vascular development by promoting vascular smooth muscle differentiation. Cell Death Differ. 2019;: pubmed publisher
Kim W, Cho Y, Wang X, Park O, Ma X, Kim H, et al. Hippo signaling is intrinsically regulated during cell cycle progression by APC/CCdh1. Proc Natl Acad Sci U S A. 2019;116:9423-9432 pubmed publisher
Tian L, Ye Z, Kafka K, Stewart D, Anders R, Schwarz K, et al. Biliary Atresia Relevant Human Induced Pluripotent Stem Cells Recapitulate Key Disease Features in a Dish. J Pediatr Gastroenterol Nutr. 2019;68:56-63 pubmed publisher
Pan J, Xiong L, Zhao K, Zeng P, Wang B, Tang F, et al. YAP promotes osteogenesis and suppresses adipogenic differentiation by regulating β-catenin signaling. Bone Res. 2018;6:18 pubmed publisher
Stammnitz M, Coorens T, Gori K, Hayes D, Fu B, Wang J, et al. The Origins and Vulnerabilities of Two Transmissible Cancers in Tasmanian Devils. Cancer Cell. 2018;33:607-619.e15 pubmed publisher
Goto H, Nishio M, To Y, Oishi T, Miyachi Y, Maehama T, et al. Loss of Mob1a/b in mice results in chondrodysplasia due to YAP1/TAZ-TEAD-dependent repression of SOX9. Development. 2018;145: pubmed publisher
Wen T, Yin Q, Yu L, Hu G, Liu J, Zhang W, et al. Characterization of mice carrying a conditional TEAD1 allele. Genesis. 2017;55: pubmed publisher
Zhao K, Shen C, Lu Y, Huang Z, Li L, Rand C, et al. Muscle Yap Is a Regulator of Neuromuscular Junction Formation and Regeneration. J Neurosci. 2017;37:3465-3477 pubmed publisher
Zhang P, Wang S, Wang S, Qiao J, Zhang L, Zhang Z, et al. Dual function of partitioning-defective 3 in the regulation of YAP phosphorylation and activation. Cell Discov. 2016;2:16021 pubmed publisher
Xu F, Ahmed A, Kang X, Hu G, Liu F, Zhang W, et al. MicroRNA-15b/16 Attenuates Vascular Neointima Formation by Promoting the Contractile Phenotype of Vascular Smooth Muscle Through Targeting YAP. Arterioscler Thromb Vasc Biol. 2015;35:2145-52 pubmed publisher
Li M, Lu J, Zhang F, Li H, Zhang B, Wu X, et al. Yes-associated protein 1 (YAP1) promotes human gallbladder tumor growth via activation of the AXL/MAPK pathway. Cancer Lett. 2014;355:201-9 pubmed publisher
Jerhammar F, Johansson A, Ceder R, Welander J, Jansson A, Grafström R, et al. YAP1 is a potential biomarker for cetuximab resistance in head and neck cancer. Oral Oncol. 2014;50:832-9 pubmed publisher
Tsujiura M, Mazack V, Sudol M, Kaspar H, Nash J, Carey D, et al. Yes-associated protein (YAP) modulates oncogenic features and radiation sensitivity in endometrial cancer. PLoS ONE. 2014;9:e100974 pubmed publisher
Lavado A, He Y, Pare J, Neale G, Olson E, Giovannini M, et al. Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators. Development. 2013;140:3323-34 pubmed publisher
Zhang N, Bai H, David K, Dong J, Zheng Y, Cai J, et al. The Merlin/NF2 tumor suppressor functions through the YAP oncoprotein to regulate tissue homeostasis in mammals. Dev Cell. 2010;19:27-38 pubmed publisher
Camargo F, Gokhale S, Johnnidis J, Fu D, Bell G, Jaenisch R, et al. YAP1 increases organ size and expands undifferentiated progenitor cells. Curr Biol. 2007;17:2054-60 pubmed
Levy D, Adamovich Y, Reuven N, Shaul Y. The Yes-associated protein 1 stabilizes p73 by preventing Itch-mediated ubiquitination of p73. Cell Death Differ. 2007;14:743-51 pubmed
Komuro A, Nagai M, Navin N, Sudol M. WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus. J Biol Chem. 2003;278:33334-41 pubmed
product information
Product No. :
WH0010413M1
Product Name :
Monoclonal Anti-YAP1 antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
2F12
Applications/Description :
Suitable for ELISA; western blot: 1-5 μg/mL; immunohistochemistry (formalin-fixed, paraffin-embedded sections); immunofluorescence
Species Reactivity :
human
Isotype :
IgG2aκ
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA