product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-KRAS antibody produced in mouse
catalog :
WH0003845M1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3B10-2F2
reactivity :
human, mouse
application :
western blot, ELISA, immunocytochemistry
more info or order :
citations: 44
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:500; loading ...; fig s8e
Castel P, Cheng A, Cuevas Navarro A, Everman D, Papageorge A, Simanshu D, et al. RIT1 oncoproteins escape LZTR1-mediated proteolysis. Science. 2019;363:1226-1230 pubmed publisher
  • immunocytochemistry; human; loading ...; tbl 1
  • ELISA; human; loading ...; tbl 1
  • western blot; human; fig 1d
Waters A, Ozkan Dagliyan I, Vaseva A, Fer N, Strathern L, Hobbs G, et al. Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. Sci Signal. 2017;10: pubmed publisher
  • western blot; human; 1:100; fig 1
  • western blot; mouse; 1:100; fig s3
White Y, Bagchi A, Van Ziffle J, Inguva A, Bollag G, Zhang C, et al. KRAS insertion mutations are oncogenic and exhibit distinct functional properties. Nat Commun. 2016;7:10647 pubmed publisher
  • western blot; human
Newman A, Scholefield C, Kemp A, Newman M, McIver E, Kamal A, et al. TBK1 kinase addiction in lung cancer cells is mediated via autophagy of Tax1bp1/Ndp52 and non-canonical NF-κB signalling. PLoS ONE. 2012;7:e50672 pubmed publisher
Amendola C, Mahaffey J, Parker S, Ahearn I, Chen W, Zhou M, et al. KRAS4A directly regulates hexokinase 1. Nature. 2019;576:482-486 pubmed publisher
Blake D, Vaseva A, Hodge R, Kline M, Gilbert T, Tyagi V, et al. Application of a MYC degradation screen identifies sensitivity to CDK9 inhibitors in KRAS-mutant pancreatic cancer. Sci Signal. 2019;12: pubmed publisher
Wang M, Fer N, Galeas J, Collisson E, Kim S, Sharib J, et al. Blockade of leukemia inhibitory factor as a therapeutic approach to KRAS driven pancreatic cancer. Nat Commun. 2019;10:3055 pubmed publisher
Guinn M, BALAZSI G. Noise-reducing optogenetic negative-feedback gene circuits in human cells. Nucleic Acids Res. 2019;: pubmed publisher
Nkembo A, Amissah F, Ntantie E, Poku R, Salako O, Ikpatt O, et al. Polyisoprenylated Cysteinyl Amide Inhibitors Deplete K-Ras and Induce Caspase-dependent Apoptosis in Lung Cancer Cells. Curr Cancer Drug Targets. 2019;19:838-851 pubmed publisher
Haghighi F, Dahlmann J, Nakhaei Rad S, Lang A, Kutschka I, Zenker M, et al. bFGF-mediated pluripotency maintenance in human induced pluripotent stem cells is associated with NRAS-MAPK signaling. Cell Commun Signal. 2018;16:96 pubmed publisher
Ferino A, Miglietta G, Picco R, Vogel S, Wengel J, Xodo L. MicroRNA therapeutics: design of single-stranded miR-216b mimics to target KRAS in pancreatic cancer cells. RNA Biol. 2018;15:1273-1285 pubmed publisher
Kam C, Dubash A, Magistrati E, Polo S, Satchell K, Sheikh F, et al. Desmoplakin maintains gap junctions by inhibiting Ras/MAPK and lysosomal degradation of connexin-43. J Cell Biol. 2018;217:3219-3235 pubmed publisher
Waters A, Bagni R, Portugal F, Hartley J. Single Synonymous Mutations in KRAS Cause Transformed Phenotypes in NIH3T3 Cells. PLoS ONE. 2016;11:e0163272 pubmed publisher
Lim Y, Bacchiocchi A, Qiu J, Straub R, Bruckner A, Bercovitch L, et al. GNA14 Somatic Mutation Causes Congenital and Sporadic Vascular Tumors by MAPK Activation. Am J Hum Genet. 2016;99:443-50 pubmed publisher
Manchado E, Weissmueller S, Morris J, Chen C, Wullenkord R, Lujambio A, et al. A combinatorial strategy for treating KRAS-mutant lung cancer. Nature. 2016;534:647-51 pubmed
Bukong T, Kodys K, Szabo G. A Novel Human Radixin Peptide Inhibits Hepatitis C Virus Infection at the Level of Cell Entry. Int J Pept Res Ther. 2014;20:269-276 pubmed
Jiao F, Hu H, Yuan C, Wang L, Jiang W, Jin Z, et al. Elevated expression level of long noncoding RNA MALAT-1 facilitates cell growth, migration and invasion in pancreatic cancer. Oncol Rep. 2014;32:2485-92 pubmed publisher
van der Ent W, Burrello C, Teunisse A, Ksander B, van der Velden P, Jager M, et al. Modeling of human uveal melanoma in zebrafish xenograft embryos. Invest Ophthalmol Vis Sci. 2014;55:6612-22 pubmed publisher
Foster J, Wu W, Scott S, Bassi M, Mohan D, Daoud Y, et al. Transforming growth factor β and insulin signal changes in stromal fibroblasts of individual keratoconus patients. PLoS ONE. 2014;9:e106556 pubmed publisher
Li Z, Jiang J, Kong W. Berberine up-regulates hepatic low-density lipoprotein receptor through Ras-independent but AMP-activated protein kinase-dependent Raf-1 activation. Biol Pharm Bull. 2014;37:1766-75 pubmed
Kweon K, Lee C, Jung S, Ban E, Kang S, Jeong J, et al. Sirt1 induction confers resistance to etoposide-induced genotoxic apoptosis in thyroid cancers. Int J Oncol. 2014;45:2065-75 pubmed publisher
Carver J, Dexheimer T, Hsu D, Weng M, Smith J, Guha R, et al. A high-throughput assay for small molecule destabilizers of the KRAS oncoprotein. PLoS ONE. 2014;9:e103836 pubmed publisher
Jiang X, Yu X, Fan J, Guo L, Zhu C, Jiang W, et al. RFT2 is overexpressed in esophageal squamous cell carcinoma and promotes tumorigenesis by sustaining cell proliferation and protecting against cell death. Cancer Lett. 2014;353:78-86 pubmed publisher
Aini W, Miyagawa Hayashino A, Ozeki M, Adeeb S, Hirata M, Tamaki K, et al. Accelerated telomere reduction and hepatocyte senescence in tolerated human liver allografts. Transpl Immunol. 2014;31:55-9 pubmed publisher
van der Ent W, Jochemsen A, Teunisse A, Krens S, Szuhai K, Spaink H, et al. Ewing sarcoma inhibition by disruption of EWSR1-FLI1 transcriptional activity and reactivation of p53. J Pathol. 2014;233:415-24 pubmed publisher
Vander Griend D, Litvinov I, Isaacs J. Conversion of androgen receptor signaling from a growth suppressor in normal prostate epithelial cells to an oncogene in prostate cancer cells involves a gain of function in c-Myc regulation. Int J Biol Sci. 2014;10:627-42 pubmed publisher
Lai P, Hsu C, Wang S, Lee J, Tseng M, Hwang J, et al. Production of a neutralizing antibody against envelope protein of dengue virus type 2 using the linear array epitope technique. J Gen Virol. 2014;95:2155-2165 pubmed publisher
Kim J, Jayaprakasha G, Patil B. Obacunone exhibits anti-proliferative and anti-aromatase activity in vitro by inhibiting the p38 MAPK signaling pathway in MCF-7 human breast adenocarcinoma cells. Biochimie. 2014;105:36-44 pubmed publisher
Yeh C, Yen C, Chen Y, Cheng C, Huang S, Chang T, et al. Identification of aurora kinase A as an unfavorable prognostic factor and potential treatment target for metastatic gastrointestinal stromal tumors. Oncotarget. 2014;5:4071-86 pubmed
Iqbal N, Xu L, Devitt C, Skapek S. Isolation and characterization of mammalian cells expressing the Arf promoter during eye development. Biotechniques. 2014;56:239-49 pubmed publisher
Brown D, LASSEGUE B, Lee M, Zafari R, Long J, Saavedra H, et al. Poldip2 knockout results in perinatal lethality, reduced cellular growth and increased autophagy of mouse embryonic fibroblasts. PLoS ONE. 2014;9:e96657 pubmed publisher
Cortez M, Valdecanas D, Zhang X, Zhan Y, Bhardwaj V, Calin G, et al. Therapeutic delivery of miR-200c enhances radiosensitivity in lung cancer. Mol Ther. 2014;22:1494-1503 pubmed publisher
Boufraqech M, Zhang L, Jain M, Patel D, Ellis R, Xiong Y, et al. miR-145 suppresses thyroid cancer growth and metastasis and targets AKT3. Endocr Relat Cancer. 2014;21:517-31 pubmed publisher
Bohanon F, Wang X, Ding C, Ding Y, Radhakrishnan G, Rastellini C, et al. Oridonin inhibits hepatic stellate cell proliferation and fibrogenesis. J Surg Res. 2014;190:55-63 pubmed publisher
Wan J, Benkdane M, Alons E, Lotersztajn S, Pavoine C. M2 kupffer cells promote hepatocyte senescence: an IL-6-dependent protective mechanism against alcoholic liver disease. Am J Pathol. 2014;184:1763-72 pubmed publisher
Song J, Zheng B, Bu X, Fei Y, Shi S. Negative association of R-Ras activation and breast cancer development. Oncol Rep. 2014;31:2776-84 pubmed publisher
Yang H, Xu W, Li Y, Lan P, Zhang J, Zhang Y, et al. Superior activity of a new histone deacetylase inhibitor (ZYJ-34c) in inhibiting growth of human leukemia cells by inducing p21WAF1 expression and cell cycle arrest. Anticancer Drugs. 2014;25:767-77 pubmed publisher
Wu X, Qi X, Liang M, Li C, Cardona C, Li D, et al. Roles of viroplasm-like structures formed by nonstructural protein NSs in infection with severe fever with thrombocytopenia syndrome virus. FASEB J. 2014;28:2504-16 pubmed publisher
Sakai H, Fujigaki H, Mazur S, Appella E. Wild-type p53-induced phosphatase 1 (Wip1) forestalls cellular premature senescence at physiological oxygen levels by regulating DNA damage response signaling during DNA replication. Cell Cycle. 2014;13:1015-29 pubmed publisher
Toulany M, Minjgee M, Saki M, Holler M, Meier F, Eicheler W, et al. ERK2-dependent reactivation of Akt mediates the limited response of tumor cells with constitutive K-RAS activity to PI3K inhibition. Cancer Biol Ther. 2014;15:317-28 pubmed publisher
He Q, Huang Y, Cai L, Zhang S, Zhang C. Expression and prognostic value of Ars2 in hepatocellular carcinoma. Int J Clin Oncol. 2014;19:880-8 pubmed publisher
Chen J, Bi H, Hou J, Zhang X, Zhang C, Yue L, et al. Atorvastatin overcomes gefitinib resistance in KRAS mutant human non-small cell lung carcinoma cells. Cell Death Dis. 2013;4:e814 pubmed publisher
Chew T, Liu X, Liu L, Spitsbergen J, Gong Z, Low B. Crosstalk of Ras and Rho: activation of RhoA abates Kras-induced liver tumorigenesis in transgenic zebrafish models. Oncogene. 2014;33:2717-27 pubmed publisher
Puebla Osorio N, Kim J, Ojeda S, Zhang H, Tavana O, Li S, et al. A novel Ku70 function in colorectal homeostasis separate from nonhomologous end joining. Oncogene. 2014;33:2748-57 pubmed publisher
product information
Product No. :
WH0003845M1
Product Name :
Monoclonal Anti-KRAS antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
3B10-2F2
Applications/Description :
Suitable for ELISA; western blot: 1-5 μg/mL; immunofluorescence; capture ELISA
Species Reactivity :
human
Isotype :
IgG1κ
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA