product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse
catalog :
T9822
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B3
reactivity :
human, mouse, rat, chicken, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 31
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:500; loading ...; fig 3
  • immunocytochemistry; human; 1:500; loading ...; fig 3
Hua K, Ferland R. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1C
Kim M, Froese C, Xie H, Trimble W. Immunofluorescent staining of septins in primary cilia. Methods Cell Biol. 2016;136:269-83 pubmed publisher
  • immunohistochemistry; human; fig 4
Airik R, Schueler M, Airik M, Cho J, Ulanowicz K, Porath J, et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS ONE. 2016;11:e0156081 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Liu Y, Lee J, Ackerman S. Mutations in the microtubule-associated protein 1A (Map1a) gene cause Purkinje cell degeneration. J Neurosci. 2015;35:4587-98 pubmed publisher
  • western blot; mouse; fig 1
Wu H, Rong Y, Correia K, Min J, Morgan J. Comparison of the enzymatic and functional properties of three cytosolic carboxypeptidase family members. J Biol Chem. 2015;290:1222-32 pubmed publisher
Gilmore Hall S, Kuo J, Ward J, Zahra R, Morrison R, Perkins G, et al. CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice. J Cell Biol. 2019;218:206-219 pubmed publisher
Pefani D, Tognoli M, Pirincci Ercan D, Gorgoulis V, O Neill E. MST2 kinase suppresses rDNA transcription in response to DNA damage by phosphorylating nucleolar histone H2B. EMBO J. 2018;37: pubmed publisher
Martin M, Veloso A, Wu J, Katrukha E, Akhmanova A. Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules. elife. 2018;7: pubmed publisher
Jenkins B, Saunders H, Record H, Johnson Schlitz D, Wildonger J. Effects of mutating ?-tubulin lysine 40 on sensory dendrite development. J Cell Sci. 2017;130:4120-4131 pubmed publisher
Herzmann S, Krumkamp R, Rode S, Kintrup C, Rumpf S. PAR-1 promotes microtubule breakdown during dendrite pruning in Drosophila. EMBO J. 2017;36:1981-1991 pubmed publisher
Rao K, Anand M, Khanna H. The carboxyl terminal mutational hotspot of the ciliary disease protein RPGRORF15 (retinitis pigmentosa GTPase regulator) is glutamylated in vivo. Biol Open. 2016;5:424-8 pubmed publisher
Shojania Feizabadi M, Janakaloti Narayanareddy B, Vadpey O, Jun Y, Chapman D, Rosenfeld S, et al. Microtubule C-Terminal Tails Can Change Characteristics of Motor Force Production. Traffic. 2015;16:1075-87 pubmed publisher
Zhang F, Su B, Wang C, Siedlak S, Mondragón Rodríguez S, Lee H, et al. Posttranslational modifications of ?-tubulin in alzheimer disease. Transl Neurodegener. 2015;4:9 pubmed publisher
Kong D, Farmer V, Shukla A, James J, Gruskin R, Kiriyama S, et al. Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles. J Cell Biol. 2014;206:855-65 pubmed publisher
Mehuron T, Kumar A, Duarte L, Yamauchi J, Accorsi A, Girgenrath M. Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy. Skelet Muscle. 2014;4:14 pubmed publisher
Airik R, Slaats G, Guo Z, Weiss A, Khan N, Ghosh A, et al. Renal-retinal ciliopathy gene Sdccag8 regulates DNA damage response signaling. J Am Soc Nephrol. 2014;25:2573-83 pubmed publisher
Yamauchi J, Kumar A, Duarte L, Mehuron T, Girgenrath M. Triggering regeneration and tackling apoptosis: a combinatorial approach to treating congenital muscular dystrophy type 1 A. Hum Mol Genet. 2013;22:4306-17 pubmed publisher
Huang J, Shan J, Kim D, Liao J, Evagelidis A, Alper S, et al. Basolateral chloride loading by the anion exchanger type 2: role in fluid secretion by the human airway epithelial cell line Calu-3. J Physiol. 2012;590:5299-316 pubmed publisher
Plotnikova O, Pugacheva E, Golemis E. Primary cilia and the cell cycle. Methods Cell Biol. 2009;94:137-60 pubmed publisher
Amoureux M, Coulibaly B, Chinot O, Loundou A, Metellus P, Rougon G, et al. Polysialic acid neural cell adhesion molecule (PSA-NCAM) is an adverse prognosis factor in glioblastoma, and regulates olig2 expression in glioma cell lines. BMC Cancer. 2010;10:91 pubmed publisher
Kozhemyakina E, Cohen T, Yao T, Lassar A. Parathyroid hormone-related peptide represses chondrocyte hypertrophy through a protein phosphatase 2A/histone deacetylase 4/MEF2 pathway. Mol Cell Biol. 2009;29:5751-62 pubmed publisher
Sánchez Cañete M, Carvalho L, Pérez Victoria F, Gamarro F, Castanys S. Low plasma membrane expression of the miltefosine transport complex renders Leishmania braziliensis refractory to the drug. Antimicrob Agents Chemother. 2009;53:1305-13 pubmed publisher
Luo Y, McDonald K, Hanrahan J. Trafficking of immature DeltaF508-CFTR to the plasma membrane and its detection by biotinylation. Biochem J. 2009;419:211-9, 2 p following 219 pubmed publisher
Lagresle Peyrou C, Six E, Picard C, Rieux Laucat F, Michel V, Ditadi A, et al. Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. Nat Genet. 2009;41:106-11 pubmed publisher
Miyagi E, Schwartzkopff F, Plishka R, Buckler White A, Clouse K, Strebel K. APOBEC3G-independent reduction in virion infectivity during long-term HIV-1 replication in terminally differentiated macrophages. Virology. 2008;379:266-74 pubmed publisher
Hutt K, Shi Z, Albertini D, Petroff B. The environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin disrupts morphogenesis of the rat pre-implantation embryo. BMC Dev Biol. 2008;8:1 pubmed publisher
Modamio Hoybjor S, Mencia A, Goodyear R, del Castillo I, Richardson G, Moreno F, et al. A mutation in CCDC50, a gene encoding an effector of epidermal growth factor-mediated cell signaling, causes progressive hearing loss. Am J Hum Genet. 2007;80:1076-89 pubmed
Ikegami K, Heier R, Taruishi M, Takagi H, Mukai M, Shimma S, et al. Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function. Proc Natl Acad Sci U S A. 2007;104:3213-8 pubmed
Soucek K, Kamaid A, Phung A, Kubala L, Bulinski J, Harper R, et al. Normal and prostate cancer cells display distinct molecular profiles of alpha-tubulin posttranslational modifications. Prostate. 2006;66:954-65 pubmed
Oakley B. Gamma-tubulin: the microtubule organizer?. Trends Cell Biol. 1992;2:1-5 pubmed
Gagnon C, White D, Cosson J, Huitorel P, Edde B, Desbruyeres E, et al. The polyglutamylated lateral chain of alpha-tubulin plays a key role in flagellar motility. J Cell Sci. 1996;109 ( Pt 6):1545-53 pubmed
product information
Product No. :
T9822
Product Name :
Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
B3
Applications/Description :
Suitable for immunocytochemistry; western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract; microarray; indirect ELISA
Species Reactivity :
mouse, Oxyrrhis marina (dinoflagellate), sea urchin (Lytechinus pictus and three others.), chicken, human, bovine, rabbit, rat, monkey
Isotype :
IgM
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA