product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-β-Tubulin IV antibody produced in mouse
catalog :
T7941
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ONS.1A6
reactivity :
human, mouse, rat, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 3b
Zhang Y, Mao D, Keeler S, Wang X, Wu K, Gerovac B, et al. Respiratory Enterovirus (Like Parainfluenza Virus) Can Cause Chronic Lung Disease If Protection by Airway Epithelial STAT1 Is Lost. J Immunol. 2019;: pubmed publisher
  • immunohistochemistry - frozen section; mouse; tbl 1
PACKARD A, Lin B, Schwob J. Sox2 and Pax6 Play Counteracting Roles in Regulating Neurogenesis within the Murine Olfactory Epithelium. PLoS ONE. 2016;11:e0155167 pubmed publisher
  • western blot; mouse; 1:5000; fig 1
Kato Y, Katsuki T, Kokubo H, Masuda A, Saga Y. Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells. Nat Commun. 2016;7:11272 pubmed publisher
  • immunocytochemistry; mouse; fig 5a
Yoshie S, Imaizumi M, Nakamura R, Otsuki K, Ikeda M, Nomoto Y, et al. Generation of airway epithelial cells with native characteristics from mouse induced pluripotent stem cells. Cell Tissue Res. 2016;364:319-30 pubmed publisher
  • western blot; human; fig 4
Wu S, Guo Z, Hopkins C, Wei N, Chu E, Wipf P, et al. Bis-cyclopropane analog of disorazole C1 is a microtubule-destabilizing agent active in ABCB1-overexpressing human colon cancer cells. Oncotarget. 2015;6:40866-79 pubmed publisher
  • immunohistochemistry; human; 1:100
Van de Laar E, Clifford M, Hasenoeder S, Kim B, Wang D, Lee S, et al. Cell surface marker profiling of human tracheal basal cells reveals distinct subpopulations, identifies MST1/MSP as a mitogenic signal, and identifies new biomarkers for lung squamous cell carcinomas. Respir Res. 2014;15:160 pubmed publisher
  • immunocytochemistry; human
de Borja Callejas F, Martinez Anton A, Alobid I, Fuentes M, Cortijo J, Picado C, et al. Reconstituted human upper airway epithelium as 3-d in vitro model for nasal polyposis. PLoS ONE. 2014;9:e100537 pubmed publisher
Yoshie S, Nakamura R, Kobayashi D, Miyake M, Omori K, Hazama A. Functional characterization of various channel-expressing central airway epithelial cells from mouse induced pluripotent stem cells. J Cell Physiol. 2019;: pubmed publisher
Helgudottir S, Lichota J, Burkhart A, Moos T. Hepcidin Mediates Transcriptional Changes in Ferroportin mRNA in Differentiated Neuronal-Like PC12 Cells Subjected to Iron Challenge. Mol Neurobiol. 2019;56:2362-2374 pubmed publisher
Deng X, Zou W, Xiong M, Wang Z, Engelhardt J, Ye S, et al. Human Parvovirus Infection of Human Airway Epithelia Induces Pyroptotic Cell Death by Inhibiting Apoptosis. J Virol. 2017;91: pubmed publisher
Gras D, Petit A, Charriot J, Knabe L, Alagha K, Gamez A, et al. Epithelial ciliated beating cells essential for ex vivo ALI culture growth. BMC Pulm Med. 2017;17:80 pubmed publisher
Kim B, Van de Laar E, Cabanero M, Tarumi S, Hasenoeder S, Wang D, et al. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis. PLoS Biol. 2016;14:e1002581 pubmed publisher
Faura Tellez G, Willemse B, Brouwer U, Nijboer Brinksma S, Vandepoele K, Noordhoek J, et al. Protocadherin-1 Localization and Cell-Adhesion Function in Airway Epithelial Cells in Asthma. PLoS ONE. 2016;11:e0163967 pubmed publisher
Hellner K, Miranda F, Fotso Chedom D, Herrero González S, Hayden D, Tearle R, et al. Premalignant SOX2 overexpression in the fallopian tubes of ovarian cancer patients: Discovery and validation studies. EBioMedicine. 2016;10:137-49 pubmed publisher
Shen W, Deng X, Zou W, Cheng F, Engelhardt J, Yan Z, et al. Identification and Functional Analysis of Novel Nonstructural Proteins of Human Bocavirus 1. J Virol. 2015;89:10097-109 pubmed publisher
Ady J, Desir S, Thayanithy V, Vogel R, Moreira A, Downey R, et al. Intercellular communication in malignant pleural mesothelioma: properties of tunneling nanotubes. Front Physiol. 2014;5:400 pubmed publisher
Zhang S, Loch A, Radtke F, Egan S, Xu K. Jagged1 is the major regulator of Notch-dependent cell fate in proximal airways. Dev Dyn. 2013;242:678-86 pubmed publisher
Krolewski R, Packard A, Jang W, Wildner H, Schwob J. Ascl1 (Mash1) knockout perturbs differentiation of nonneuronal cells in olfactory epithelium. PLoS ONE. 2012;7:e51737 pubmed publisher
Pasquier E, Tuset M, Street J, Sinnappan S, MacKenzie K, Braguer D, et al. Concentration- and schedule-dependent effects of chemotherapy on the angiogenic potential and drug sensitivity of vascular endothelial cells. Angiogenesis. 2013;16:373-86 pubmed publisher
Roales Buján R, Páez P, Guerra M, Rodriguez S, Vio K, Ho Plagaro A, et al. Astrocytes acquire morphological and functional characteristics of ependymal cells following disruption of ependyma in hydrocephalus. Acta Neuropathol. 2012;124:531-46 pubmed publisher
Choi J, Lee J, Kim Y. Expression of ?-tubulin isotypes in classical Hodgkin's lymphoma. Pathol Int. 2012;62:287-90 pubmed publisher
Stewart C, Torr E, Mohd Jamili N, Bosquillon C, Sayers I. Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research. J Allergy (Cairo). 2012;2012:943982 pubmed publisher
Holbrook E, Wu E, Curry W, Lin D, Schwob J. Immunohistochemical characterization of human olfactory tissue. Laryngoscope. 2011;121:1687-701 pubmed publisher
Schmidt K, Wies E, Neipel F. Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor 3 inhibits gamma interferon and major histocompatibility complex class II expression. J Virol. 2011;85:4530-7 pubmed publisher
Galabova Kovacs G, Baccarini M. Deciphering signaling pathways in vivo: the Ras/Raf/MEK/ERK cascade. Methods Mol Biol. 2010;661:421-31 pubmed publisher
McCarroll J, Gan P, Liu M, Kavallaris M. betaIII-tubulin is a multifunctional protein involved in drug sensitivity and tumorigenesis in non-small cell lung cancer. Cancer Res. 2010;70:4995-5003 pubmed publisher
Nutu M, Weijdegård B, Thomas P, Thurin Kjellberg A, Billig H, Larsson D. Distribution and hormonal regulation of membrane progesterone receptors beta and gamma in ciliated epithelial cells of mouse and human fallopian tubes. Reprod Biol Endocrinol. 2009;7:89 pubmed publisher
Shao R, Nutu M, Karlsson Lindahl L, Benrick A, Weijdegård B, Lager S, et al. Downregulation of cilia-localized Il-6R alpha by 17beta-estradiol in mouse and human fallopian tubes. Am J Physiol Cell Physiol. 2009;297:C140-51 pubmed publisher
Takaki E, Fujimoto M, Nakahari T, Yonemura S, Miyata Y, Hayashida N, et al. Heat shock transcription factor 1 is required for maintenance of ciliary beating in mice. J Biol Chem. 2007;282:37285-92 pubmed
Vent J, Wyatt T, Smith D, Banerjee A, Luduena R, Sisson J, et al. Direct involvement of the isotype-specific C-terminus of beta tubulin in ciliary beating. J Cell Sci. 2005;118:4333-41 pubmed
Roach M, Boucher V, Walss C, Ravdin P, Luduena R. Preparation of a monoclonal antibody specific for the class I isotype of beta-tubulin: the beta isotypes of tubulin differ in their cellular distributions within human tissues. Cell Motil Cytoskeleton. 1998;39:273-85 pubmed
Khan I, Luduena R. Phosphorylation of beta III-tubulin. Biochemistry. 1996;35:3704-11 pubmed
Renthal R, Schneider B, Miller M, Luduena R. Beta IV is the major beta-tubulin isotype in bovine cilia. Cell Motil Cytoskeleton. 1993;25:19-29 pubmed
Sharma J, Luduena R. Use of N,N'-polymethylenebis(iodoacetamide) derivatives as probes for the detection of conformational differences in tubulin isotypes. J Protein Chem. 1994;13:165-76 pubmed
Speirs R, Welch J, Cynamon M. Activity of n-propyl pyrazinoate against pyrazinamide-resistant Mycobacterium tuberculosis: investigations into mechanism of action of and mechanism of resistance to pyrazinamide. Antimicrob Agents Chemother. 1995;39:1269-71 pubmed
Banerjee A, Luduena R. Kinetics of colchicine binding to purified beta-tubulin isotypes from bovine brain. J Biol Chem. 1992;267:13335-9 pubmed
Banerjee A, Roach M, Trcka P, Luduena R. Preparation of a monoclonal antibody specific for the class IV isotype of beta-tubulin. Purification and assembly of alpha beta II, alpha beta III, and alpha beta IV tubulin dimers from bovine brain. J Biol Chem. 1992;267:5625-30 pubmed
product information
Product No. :
T7941
Product Name :
Monoclonal Anti-β-Tubulin IV antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
ONS.1A6
Applications/Description :
Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections); western blot: 1:400 using cultured human breast adenocarcinoma cell line MCF-7; microarray; indirect ELISA
Species Reactivity :
chicken, human, hamster, bovine, mouse, rat
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA