product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-γ-Tubulin antibody produced in mouse
catalog :
T5326
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GTU-88
reactivity :
Candida albicans, African green monkey, human, mouse, rat, dogs, chicken, bovine, zebrafish , fruit fly , Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 408
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly ; 1:500; loading ...; fig 2c
Bouska M, Bai H. Long noncoding RNA regulation of spermatogenesis via the spectrin cytoskeleton in Drosophila. G3 (Bethesda). 2021;11: pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1e''
Qiu H, Fujisawa S, Nozaki S, Katoh Y, Nakayama K. Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry. Biol Open. 2021;10: pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; fig 2a
Zurli V, Montecchi T, Heilig R, Poschke I, Volkmar M, Wimmer G, et al. Phosphoproteomics of CD2 signaling reveals AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells. Sci Signal. 2020;13: pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; fig s2d
Boukhalfa A, Nascimbeni A, Ramel D, Dupont N, Hirsch E, Gayral S, et al. PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Nat Commun. 2020;11:294 pubmed publisher
  • western blot; rat; loading ...; fig 1b
Lundby A, Franciosa G, Emdal K, Refsgaard J, Gnosa S, Bekker Jensen D, et al. Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites. Cell. 2019;179:543-560.e26 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2c
Hartono -, Hazawa M, Lim K, Dewi F, Kobayashi A, Wong R. Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis. Cell Div. 2019;14:7 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; loading ...; fig s5c
Sun T, Song Y, Dai J, Mao D, Ma M, Ni J, et al. Spectraplakin Shot Maintains Perinuclear Microtubule Organization in Drosophila Polyploid Cells. Dev Cell. 2019;49:731-747.e7 pubmed publisher
  • western blot; human; loading ...; fig 5a
Davis M, Fairgrieve M, den Hartigh A, Yakovenko O, Duvvuri B, Lood C, et al. Calpain drives pyroptotic vimentin cleavage, intermediate filament loss, and cell rupture that mediates immunostimulation. Proc Natl Acad Sci U S A. 2019;116:5061-5070 pubmed publisher
  • immunocytochemistry; human; 1 ug/ml; loading ...; fig 1c
Malaby H, Dumas M, Ohi R, Stumpff J. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. J Cell Biol. 2019;218:1218-1234 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:5000; fig 4e
Song P, Perkins B. Developmental expression of the zebrafish Arf-like small GTPase paralogs arl13a and arl13b. Gene Expr Patterns. 2018;29:82-87 pubmed publisher
  • immunocytochemistry; human; 1:4000; loading ...; fig 1a
Conkar D, Culfa E, Odabasi E, Rauniyar N, Yates J, Firat Karalar E. The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking. J Cell Sci. 2017;130:1450-1462 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 3a
Funabashi T, Katoh Y, Michisaka S, Terada M, Sugawa M, Nakayama K. Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol Biol Cell. 2017;28:624-633 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 8d
Sierra Potchanant E, Cerabona D, Sater Z, He Y, Sun Z, Gehlhausen J, et al. INPP5E Preserves Genomic Stability through Regulation of Mitosis. Mol Cell Biol. 2017;37: pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 4a'
Hirano T, Katoh Y, Nakayama K. Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol Biol Cell. 2017;28:429-439 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 4
Meitinger F, Anzola J, Kaulich M, Richardson A, Stender J, Benner C, et al. 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol. 2016;214:155-66 pubmed publisher
  • western blot; human; 1:4000; fig 3d
Zhang X, Cheng X, Yu L, Yang J, Calvo R, Patnaik S, et al. MCOLN1 is a ROS sensor in lysosomes that regulates autophagy. Nat Commun. 2016;7:12109 pubmed publisher
  • immunocytochemistry; mouse; fig 3
  • western blot; mouse; fig 7
  • western blot; human; fig s3
Muroyama A, Seldin L, Lechler T. Divergent regulation of functionally distinct γ-tubulin complexes during differentiation. J Cell Biol. 2016;213:679-92 pubmed publisher
  • immunohistochemistry; human; fig 4
Airik R, Schueler M, Airik M, Cho J, Ulanowicz K, Porath J, et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS ONE. 2016;11:e0156081 pubmed publisher
  • western blot; rat; fig 1
Meek S, Thomson A, Sutherland L, Sharp M, Thomson J, Bishop V, et al. Reduced levels of dopamine and altered metabolism in brains of HPRT knock-out rats: a new rodent model of Lesch-Nyhan Disease. Sci Rep. 2016;6:25592 pubmed publisher
  • western blot; human; 1:500; fig s1
Rao S, Flores Rodriguez N, Page S, Wong C, Robinson P, Chircop M. The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics. 2016;15:2537-53 pubmed publisher
  • immunocytochemistry; human; fig 1
  • immunocytochemistry; mouse; fig 2
Cooley L, El Shikh M, Li W, Keim R, Zhang Z, Strauss J, et al. Impaired immunological synapse in sperm associated antigen 6 (SPAG6) deficient mice. Sci Rep. 2016;6:25840 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 5c
Shahbazi M, Jedrusik A, Vuoristo S, Recher G, Hupalowska A, Bolton V, et al. Self-organization of the human embryo in the absence of maternal tissues. Nat Cell Biol. 2016;18:700-708 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; fig 2
  • immunohistochemistry; mouse; 1:1000; fig 2
Noda K, Kitami M, Kitami K, Kaku M, Komatsu Y. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development. Proc Natl Acad Sci U S A. 2016;113:E2589-97 pubmed publisher
  • western blot; human; fig 1
  • western blot; mouse; fig 1
West A, Martin B, Andrews D, Hogg S, Banerjee A, Grigoriadis G, et al. The SMAC mimetic, LCL-161, reduces survival in aggressive MYC-driven lymphoma while promoting susceptibility to endotoxic shock. Oncogenesis. 2016;5:e216 pubmed publisher
  • immunocytochemistry; mouse; fig 7
Yadav P, Selvaraj B, Bender F, Behringer M, Moradi M, Sivadasan R, et al. Neurofilament depletion improves microtubule dynamics via modulation of Stat3/stathmin signaling. Acta Neuropathol. 2016;132:93-110 pubmed publisher
  • western blot; mouse; fig 7
Braun J, Meixner A, Brachner A, Foisner R. The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis. PLoS ONE. 2016;11:e0152278 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2cd
San Agustin J, Klena N, Granath K, Panigrahy A, Stewart E, Devine W, et al. Genetic link between renal birth defects and congenital heart disease. Nat Commun. 2016;7:11103 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 4
  • western blot; human; 1:1000; fig 1
Chavali P, Chandrasekaran G, Barr A, Tátrai P, Taylor C, Papachristou E, et al. A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer. Nat Commun. 2016;7:11005 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 6g
Katoh Y, Terada M, Nishijima Y, Takei R, Nozaki S, Hamada H, et al. Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex. J Biol Chem. 2016;291:10962-75 pubmed publisher
  • western blot; mouse; 1:1000; fig 2
Hasegawa K, Yasuda T, Shiraishi C, Fujiwara K, Przedborski S, Mochizuki H, et al. Promotion of mitochondrial biogenesis by necdin protects neurons against mitochondrial insults. Nat Commun. 2016;7:10943 pubmed publisher
  • western blot; human; 1:500; fig 6
Li X, Rydzewski N, Hider A, Zhang X, Yang J, Wang W, et al. A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation. Nat Cell Biol. 2016;18:404-17 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:400; fig 1c
Di Pietro C, Marazziti D, La Sala G, Abbaszadeh Z, Golini E, Matteoni R, et al. Primary Cilia in the Murine Cerebellum and in Mutant Models of Medulloblastoma. Cell Mol Neurobiol. 2017;37:145-154 pubmed publisher
  • western blot; human; fig 1
Wild T, Larsen M, Narita T, Schou J, Nilsson J, Choudhary C. The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity. Cell Rep. 2016;14:1829-40 pubmed publisher
  • western blot; mouse; fig 5d
Kibe T, Zimmermann M, de Lange T. TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres. Mol Cell. 2016;61:236-46 pubmed publisher
  • western blot; human; fig 1b
Hérate C, Ramdani G, Grant N, Marion S, Gasman S, Niedergang F, et al. Phospholipid Scramblase 1 Modulates FcR-Mediated Phagocytosis in Differentiated Macrophages. PLoS ONE. 2016;11:e0145617 pubmed publisher
  • immunocytochemistry; mouse; 1:200-1:500; fig 4
Drusenheimer N, Migdal B, Jäckel S, Tveriakhina L, Scheider K, Schulz K, et al. The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling. PLoS Genet. 2015;11:e1005749 pubmed publisher
  • immunocytochemistry; human; fig 4
Toya M, Kobayashi S, Kawasaki M, Shioi G, Kaneko M, Ishiuchi T, et al. CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells. Proc Natl Acad Sci U S A. 2016;113:332-7 pubmed publisher
  • western blot; mouse; 1:3000; loading ...; fig 1c
Brown K, Selfridge J, Lagger S, Connelly J, De Sousa D, Kerr A, et al. The molecular basis of variable phenotypic severity among common missense mutations causing Rett syndrome. Hum Mol Genet. 2016;25:558-70 pubmed publisher
  • immunocytochemistry; human; 1:250; fig 1
Tollenaere M, Villumsen B, Blasius M, Nielsen J, Wagner S, Bartek J, et al. p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nat Commun. 2015;6:10075 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; fig 5
Gazea M, Tasouri E, Tolve M, Bosch V, Kabanova A, Gojak C, et al. Primary cilia are critical for Sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain. Dev Biol. 2016;409:55-71 pubmed publisher
  • immunohistochemistry - paraffin section; fruit fly ; 1:100; loading ...
Tarayrah L, Li Y, Gan Q, Chen X. Epigenetic regulator Lid maintains germline stem cells through regulating JAK-STAT signaling pathway activity. Biol Open. 2015;4:1518-27 pubmed publisher
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 2
Magron A, Elowe S, Carreau M. The Fanconi Anemia C Protein Binds to and Regulates Stathmin-1 Phosphorylation. PLoS ONE. 2015;10:e0140612 pubmed publisher
  • immunohistochemistry; mouse
Arya P, Rainey M, Bhattacharyya S, Mohapatra B, George M, Kuracha M, et al. The endocytic recycling regulatory protein EHD1 Is required for ocular lens development. Dev Biol. 2015;408:41-55 pubmed publisher
  • immunohistochemistry; human
Stephen L, Tawamie H, Davis G, Tebbe L, Nurnberg P, Nurnberg G, et al. TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23). elife. 2015;4: pubmed publisher
  • immunocytochemistry; human; 1:5000; fig s2
Van de Mark D, Kong D, Loncarek J, Stearns T. MDM1 is a microtubule-binding protein that negatively regulates centriole duplication. Mol Biol Cell. 2015;26:3788-802 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 2a
  • immunohistochemistry; human; 1:1000; fig 2a
Hu S, Lu Y, Orr B, Godek K, Mustachio L, Kawakami M, et al. Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown. Mol Cancer Ther. 2015;14:2576-85 pubmed publisher
  • immunocytochemistry; human; 1:200; fig s9
Wong F, Fei J, Mora Bermúdez F, Taverna E, Haffner C, Fu J, et al. Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex. PLoS Biol. 2015;13:e1002217 pubmed publisher
  • immunocytochemistry; Xenopus laevis; 1:100; fig 1
Dubińska Magiera M, Chmielewska M, Kozioł K, Machowska M, Hutchison C, Goldberg M, et al. Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability. Protoplasma. 2016;253:943-56 pubmed publisher
  • immunohistochemistry; mouse; 1:500; fig 3c
Rujano M, Basto R, Marthiens V. New insights into centrosome imaging in Drosophila and mouse neuroepithelial tissues. Methods Cell Biol. 2015;129:211-227 pubmed publisher
  • western blot; human; 1:4000; fig 1
Kontro H, Cannino G, Rustin P, Dufour E, Kainulainen H. DAPIT Over-Expression Modulates Glucose Metabolism and Cell Behaviour in HEK293T Cells. PLoS ONE. 2015;10:e0131990 pubmed publisher
  • immunocytochemistry; chicken; fig 5
Burgess S, Peset I, Joseph N, Cavazza T, Vernos I, Pfuhl M, et al. Aurora-A-Dependent Control of TACC3 Influences the Rate of Mitotic Spindle Assembly. PLoS Genet. 2015;11:e1005345 pubmed publisher
  • western blot; human
Romano S, Xiao Y, Nakaya M, D Angelillo A, Chang M, Jin J, et al. FKBP51 employs both scaffold and isomerase functions to promote NF-κB activation in melanoma. Nucleic Acids Res. 2015;43:6983-93 pubmed publisher
  • western blot; mouse; fig 5
Woodcroft M, Nanan K, Thompson P, Tyryshkin K, Smith S, Slany R, et al. Retrovirus-Mediated Expression of E2A-PBX1 Blocks Lymphoid Fate but Permits Retention of Myeloid Potential in Early Hematopoietic Progenitors. PLoS ONE. 2015;10:e0130495 pubmed publisher
  • western blot; rat; 1:10,000; fig 6
Götze S, Schumacher E, Kordes C, Häussinger D. Epigenetic Changes during Hepatic Stellate Cell Activation. PLoS ONE. 2015;10:e0128745 pubmed publisher
  • western blot; human; 1:2000
Barr A, Bakal C. A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly. Sci Rep. 2015;5:10564 pubmed publisher
  • western blot; mouse; fig 1
Dennis M, Mantegazza A, Snir O, Tenza D, Acosta Ruiz A, Delevoye C, et al. BLOC-2 targets recycling endosomal tubules to melanosomes for cargo delivery. J Cell Biol. 2015;209:563-77 pubmed publisher
  • western blot; human; fig 3
Palla A, Piazzolla D, Alcazar N, Cañamero M, Graña O, Gómez López G, et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep. 2015;5:10205 pubmed publisher
  • western blot; human; fig 5
Batenburg N, Thompson E, Hendrickson E, Zhu X. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015;34:1399-416 pubmed publisher
  • western blot; mouse; 1:500; fig 3
Boersma V, Moatti N, Segura Bayona S, Peuscher M, van der Torre J, Wevers B, et al. MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection. Nature. 2015;521:537-540 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 7
Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun. 2015;6:6366 pubmed publisher
  • immunocytochemistry; bovine; fig s5
Marsolier J, Perichon M, Debarry J, Villoutreix B, Chluba J, Lopez T, et al. Theileria parasites secrete a prolyl isomerase to maintain host leukocyte transformation. Nature. 2015;520:378-82 pubmed publisher
  • immunocytochemistry; human; 1:500; fig s4
Ochi T, Blackford A, Coates J, Jhujh S, Mehmood S, Tamura N, et al. DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015;347:185-188 pubmed publisher
  • immunocytochemistry; human
Shibata H, Kanadome T, Sugiura H, Yokoyama T, Yamamuro M, Moss S, et al. A new role for annexin A11 in the early secretory pathway via stabilizing Sec31A protein at the endoplasmic reticulum exit sites (ERES). J Biol Chem. 2015;290:4981-93 pubmed publisher
  • western blot; human; 1:1000; fig 2
Pagan J, Marzio A, Jones M, Saraf A, Jallepalli P, Florens L, et al. Degradation of Cep68 and PCNT cleavage mediate Cep215 removal from the PCM to allow centriole separation, disengagement and licensing. Nat Cell Biol. 2015;17:31-43 pubmed publisher
  • immunocytochemistry; human; 1:2000; fig 1a
Rao Y, Hao R, Wang B, Yao T. A Mec17-Myosin II Effector Axis Coordinates Microtubule Acetylation and Actin Dynamics to Control Primary Cilium Biogenesis. PLoS ONE. 2014;9:e114087 pubmed publisher
  • immunocytochemistry; human; fig 1
Zou J, Zhang D, Qin G, Chen X, Wang H, Zhang D. BRCA1 and FancJ cooperatively promote interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1. Cell Cycle. 2014;13:3685-97 pubmed publisher
  • western blot; mouse; fig 2
Pekkonen P, Järviluoma A, Zinovkina N, Cvrljevic A, Prakash S, Westermarck J, et al. KSHV viral cyclin interferes with T-cell development and induces lymphoma through Cdk6 and Notch activation in vivo. Cell Cycle. 2014;13:3670-84 pubmed publisher
  • immunocytochemistry; human; fig 9
Colbert P, Vermeer D, Wieking B, Lee J, Vermeer P. EphrinB1: novel microtubule associated protein whose expression affects taxane sensitivity. Oncotarget. 2015;6:953-68 pubmed
  • western blot; Candida albicans; 1:5000; fig 3
Olmsted Z, Colliver A, Riehlman T, Paluh J. Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells. Nat Commun. 2014;5:5339 pubmed publisher
  • immunocytochemistry; mouse
Brown J, Santra T, Owens P, Morrison A, Barry F. Primary cilium-associated genes mediate bone marrow stromal cell response to hypoxia. Stem Cell Res. 2014;13:284-99 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; fig 2
Lee Y, Santé J, Comerci C, Cyge B, Menezes L, Li F, et al. Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function. Mol Biol Cell. 2014;25:2919-33 pubmed publisher
  • western blot; human
Izumi H, Kaneko Y. Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells. Cancer Res. 2014;74:5620-30 pubmed publisher
  • western blot; human; fig s5
Lima W, Vickers T, Nichols J, Li C, Crooke S. Defining the factors that contribute to on-target specificity of antisense oligonucleotides. PLoS ONE. 2014;9:e101752 pubmed publisher
  • immunocytochemistry; human; 1:2000
Kotak S, Busso C, GONCZY P. NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane. EMBO J. 2014;33:1815-30 pubmed publisher
  • western blot; mouse; 1:10,000; fig 7
Piazzolla D, Palla A, Pantoja C, Canamero M, de Castro I, Ortega S, et al. Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia. Nat Commun. 2014;5:4226 pubmed publisher
  • western blot; human
Failler M, Gee H, Krug P, Joo K, Halbritter J, Belkacem L, et al. Mutations of CEP83 cause infantile nephronophthisis and intellectual disability. Am J Hum Genet. 2014;94:905-14 pubmed publisher
  • immunocytochemistry; African green monkey; 1:800
Kielar M, Tuy F, Bizzotto S, Lebrand C, de Juan Romero C, Poirier K, et al. Mutations in Eml1 lead to ectopic progenitors and neuronal heterotopia in mouse and human. Nat Neurosci. 2014;17:923-33 pubmed publisher
  • immunocytochemistry; human; 1:200
  • western blot; human
Kuo P, Huang Y, Hsieh C, Lee J, Lin B, Hung L. STK31 is a cell-cycle regulated protein that contributes to the tumorigenicity of epithelial cancer cells. PLoS ONE. 2014;9:e93303 pubmed publisher
  • immunocytochemistry; human; 1:1000
Lee J, Hong W, Majeti R, Stearns T. Centrosome-kinase fusions promote oncogenic signaling and disrupt centrosome function in myeloproliferative neoplasms. PLoS ONE. 2014;9:e92641 pubmed publisher
  • western blot; human
Cheng Y, Holloway M, Nguyen K, McCauley D, Landesman Y, Kauffman M, et al. XPO1 (CRM1) inhibition represses STAT3 activation to drive a survivin-dependent oncogenic switch in triple-negative breast cancer. Mol Cancer Ther. 2014;13:675-86 pubmed publisher
  • immunocytochemistry; human; 1:500
Polling S, Mok Y, Ramdzan Y, Turner B, Yerbury J, Hill A, et al. Misfolded polyglutamine, polyalanine, and superoxide dismutase 1 aggregate via distinct pathways in the cell. J Biol Chem. 2014;289:6669-80 pubmed publisher
  • western blot; human
Feng Y, Wu H, Xu Y, Zhang Z, Liu T, Lin X, et al. Zinc finger protein 451 is a novel Smad corepressor in transforming growth factor-? signaling. J Biol Chem. 2014;289:2072-83 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 5
Lewis S, Hedman C, Ziegler T, Ricke W, Jorgensen J. Steroidogenic factor 1 promotes aggressive growth of castration-resistant prostate cancer cells by stimulating steroid synthesis and cell proliferation. Endocrinology. 2014;155:358-69 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
Hashizume C, Kobayashi A, Wong R. Down-modulation of nucleoporin RanBP2/Nup358 impaired chromosomal alignment and induced mitotic catastrophe. Cell Death Dis. 2013;4:e854 pubmed publisher
  • immunocytochemistry; human
Hashizume C, Moyori A, Kobayashi A, Yamakoshi N, Endo A, Wong R. Nucleoporin Nup62 maintains centrosome homeostasis. Cell Cycle. 2013;12:3804-16 pubmed publisher
  • immunocytochemistry; human; fig 3a
Zhang Y, Seo S, Bhattarai S, Bugge K, Searby C, Zhang Q, et al. BBS mutations modify phenotypic expression of CEP290-related ciliopathies. Hum Mol Genet. 2014;23:40-51 pubmed publisher
  • western blot; human; fig 5
Dai J, Brooks Y, Lefort K, Getsios S, Dotto G. The retinoid-related orphan receptor ROR? promotes keratinocyte differentiation via FOXN1. PLoS ONE. 2013;8:e70392 pubmed publisher
  • immunocytochemistry; human
Boutros R, Mondesert O, Lorenzo C, Astuti P, McArthur G, Chircop M, et al. CDC25B overexpression stabilises centrin 2 and promotes the formation of excess centriolar foci. PLoS ONE. 2013;8:e67822 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000
Willaredt M, Gorgas K, Gardner H, Tucker K. Multiple essential roles for primary cilia in heart development. Cilia. 2012;1:23 pubmed publisher
  • western blot; human
Koreishi M, Yu S, Oda M, Honjo Y, Satoh A. CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. PLoS ONE. 2013;8:e54382 pubmed publisher
  • immunocytochemistry; chicken; 1:150
Wang Y, Dantas T, Lalor P, Dockery P, Morrison C. Promoter hijack reveals pericentrin functions in mitosis and the DNA damage response. Cell Cycle. 2013;12:635-46 pubmed publisher
  • immunocytochemistry; mouse
Zimmermann M, Lottersberger F, Buonomo S, Sfeir A, de Lange T. 53BP1 regulates DSB repair using Rif1 to control 5' end resection. Science. 2013;339:700-4 pubmed publisher
  • western blot; human
Jullien L, Mestre M, Roux P, Gire V. Eroded human telomeres are more prone to remain uncapped and to trigger a G2 checkpoint response. Nucleic Acids Res. 2013;41:900-11 pubmed publisher
  • immunocytochemistry; human; 1:500
Conroy P, Saladino C, Dantas T, Lalor P, Dockery P, Morrison C. C-NAP1 and rootletin restrain DNA damage-induced centriole splitting and facilitate ciliogenesis. Cell Cycle. 2012;11:3769-78 pubmed publisher
  • immunocytochemistry; human
Ma M, Chircop M. SNX9, SNX18 and SNX33 are required for progression through and completion of mitosis. J Cell Sci. 2012;125:4372-82 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 4
Reboutier D, Troadec M, Cremet J, Fukasawa K, Prigent C. Nucleophosmin/B23 activates Aurora A at the centrosome through phosphorylation of serine 89. J Cell Biol. 2012;197:19-26 pubmed publisher
  • western blot; human
Chen Y, Fang S, Yeh P, Yang H, Chen S, Chang C, et al. The C-terminus of PARK2 is required for its self-interaction, solubility and role in the spindle assembly checkpoint. Biochim Biophys Acta. 2012;1822:573-80 pubmed publisher
Schink K, Tan K, Spangenberg H, Martorana D, Sneeggen M, Stévenin V, et al. The phosphoinositide coincidence detector Phafin2 promotes macropinocytosis by coordinating actin organisation at forming macropinosomes. Nat Commun. 2021;12:6577 pubmed publisher
Santoliquido B, Frenquelli M, Contadini C, Bestetti S, Gaviraghi M, Barbieri E, et al. Deletion of a pseudogene within a fragile site triggers the oncogenic expression of the mitotic CCSER1 gene. Life Sci Alliance. 2021;4: pubmed publisher
Bowman S, Le L, Zhu Y, Harper D, Sitaram A, Theos A, et al. A BLOC-1-AP-3 super-complex sorts a cis-SNARE complex into endosome-derived tubular transport carriers. J Cell Biol. 2021;220: pubmed publisher
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product information
Product No. :
T5326
Product Name :
Monoclonal Anti-γ-Tubulin antibody produced in mouse
Host :
mouse
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
GTU-88
Applications/Description :
Suitable for immunocytochemistry: 1-2 μg/mL using HeLa cells; western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts; microarray; indirect ELISA; immunoprecipitation (IP)
Species Reactivity :
Xenopus, canine, chicken, hamster, rat, human, bovine, mouse
Isotype :
IgG1
Concentration :
~1 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA