product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-α-Tubulin antibody produced in mouse
catalog :
T5168
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B-5-1-2
reactivity :
Saccharomycetales, fission yeast, hamsters, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, zebrafish , fruit fly , domestic sheep, Xenopus laevis, thale cress, soybeans, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, radioimmunoassay, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, reverse phase protein lysate microarray
more info or order :
citations: 2591
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:5000; loading ...; fig 2f
Lechertier T, Reynolds L, Kim H, Pedrosa A, Gómez Escudero J, Muñoz Félix J, et al. Pericyte FAK negatively regulates Gas6/Axl signalling to suppress tumour angiogenesis and tumour growth. Nat Commun. 2020;11:2810 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 1b
Bozic M, Caus M, Rodrigues Díez R, Pedraza N, Ruiz Ortega M, Garí E, et al. Protective role of renal proximal tubular alpha-synuclein in the pathogenesis of kidney fibrosis. Nat Commun. 2020;11:1943 pubmed publisher
  • western blot; human; loading ...; fig 5
Leite M, Marques M, Melo J, Pinto M, Cavadas B, Aroso M, et al. Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions. Cells. 2020;9: pubmed publisher
  • western blot; mouse; 1:5000; loading ...; fig 5g
Sciascia N, Wu W, Zong D, Sun Y, Wong N, John S, et al. Suppressing proteasome mediated processing of Topoisomerase II DNA-protein complexes preserves genome integrity. elife. 2020;9: pubmed publisher
  • immunohistochemistry; mouse; 1:5000; loading ...; fig 4f
Zhao L, Ke H, Xu H, Wang G, Zhang H, Zou L, et al. TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh. Nat Commun. 2020;11:341 pubmed publisher
  • western blot; mouse; loading ...; fig 4b
Naiman S, Huynh F, Gil R, Glick Y, Shahar Y, Touitou N, et al. SIRT6 Promotes Hepatic Beta-Oxidation via Activation of PPARα. Cell Rep. 2019;29:4127-4143.e8 pubmed publisher
  • western blot; mouse; 1:10,000; fig 1b
Nakamura Y, Dryanovski D, Kimura Y, Jackson S, Woods A, Yasui Y, et al. Cocaine-induced endocannabinoid signaling mediated by sigma-1 receptors and extracellular vesicle secretion. elife. 2019;8: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6c
Arrondel C, Missoury S, Snoek R, Patat J, Menara G, Collinet B, et al. Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun. 2019;10:3967 pubmed publisher
  • western blot; human; loading ...; fig 3f
Sanghvi V, Leibold J, Mina M, Mohan P, Berishaj M, Li Z, et al. The Oncogenic Action of NRF2 Depends on De-glycation by Fructosamine-3-Kinase. Cell. 2019;178:807-819.e21 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1d
Ommer A, Figlia G, Pereira J, Datwyler A, Gerber J, Degeer J, et al. Ral GTPases in Schwann cells promote radial axonal sorting in the peripheral nervous system. J Cell Biol. 2019;: pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 1b
Sauer M, Juranek S, Marks J, De Magis A, Kazemier H, Hilbig D, et al. DHX36 prevents the accumulation of translationally inactive mRNAs with G4-structures in untranslated regions. Nat Commun. 2019;10:2421 pubmed publisher
  • western blot; human; 1:10,000; loading ...; fig 6b
Mikuličić S, Finke J, Boukhallouk F, Wüstenhagen E, Sons D, Homsi Y, et al. ADAM17-dependent signaling is required for oncogenic human papillomavirus entry platform assembly. elife. 2019;8: pubmed publisher
  • western blot; human; loading ...; fig 7d
Gregersen L, Mitter R, Ugalde A, Nojima T, Proudfoot N, Agami R, et al. SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Cell. 2019;: pubmed publisher
  • western blot; fruit fly ; loading ...; fig 1s1b
Weiss S, Melom J, Ormerod K, Zhang Y, Littleton J. Glial Ca2+signaling links endocytosis to K+ buffering around neuronal somas to regulate excitability. elife. 2019;8: pubmed publisher
  • western blot; mouse; 1:5000; loading ...; fig 6d
Wu W, Zhang W, Choi M, Zhao J, Gao P, Xue M, et al. Vascular smooth muscle-MAPK14 is required for neointimal hyperplasia by suppressing VSMC differentiation and inducing proliferation and inflammation. Redox Biol. 2019;22:101137 pubmed publisher
  • western blot; human; loading ...; fig 6b
Kubli S, Bassi C, Roux C, Wakeham A, Göbl C, Zhou W, et al. AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer. Proc Natl Acad Sci U S A. 2019;116:3604-3613 pubmed publisher
  • western blot; human; loading ...; fig 1a
Paul D, Islam S, Manne R, Dinesh U, Malonia S, Maity B, et al. F-box protein FBXO16 functions as a tumor suppressor by attenuating nuclear β-catenin function. J Pathol. 2019;248:266-279 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 7a
  • western blot; human; 1:2000; loading ...; fig 5b
Song K, Gras C, Capin G, Gimber N, Lehmann M, Mohd S, et al. A SEPT1-based scaffold is required for Golgi integrity and function. J Cell Sci. 2019;132: pubmed publisher
  • western blot; mouse; loading ...; fig 2d
Guo J, Dai X, Laurent B, Zheng N, Gan W, Zhang J, et al. AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions. Nat Cell Biol. 2019;21:226-237 pubmed publisher
  • western blot; human; 1:10,000; loading ...; fig 4e
Fenderico N, van Scherpenzeel R, Goldflam M, Proverbio D, Jordens I, Kralj T, et al. Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells. Nat Commun. 2019;10:365 pubmed publisher
  • western blot; human; loading ...; fig 3d
Vítor A, Sridhara S, Sabino J, Afonso A, Grosso A, Martin R, et al. Single-molecule imaging of transcription at damaged chromatin. Sci Adv. 2019;5:eaau1249 pubmed publisher
  • western blot; human; loading ...; fig 8a
Li Z, Mbah N, Overmeyer J, Sarver J, George S, Trabbic C, et al. The JNK signaling pathway plays a key role in methuosis (non-apoptotic cell death) induced by MOMIPP in glioblastoma. BMC Cancer. 2019;19:77 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 5i
Wintgens J, Wichert S, Popovic L, Rossner M, Wehr M. Monitoring activities of receptor tyrosine kinases using a universal adapter in genetically encoded split TEV assays. Cell Mol Life Sci. 2019;76:1185-1199 pubmed publisher
  • western blot; human; 1:3000; loading ...; fig s2d
Silberman A, Goldman O, Boukobza Assayag O, Jacob A, Rabinovich S, Adler L, et al. Acid-Induced Downregulation of ASS1 Contributes to the Maintenance of Intracellular pH in Cancer. Cancer Res. 2019;79:518-533 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s4b
  • western blot; human; loading ...; fig 3c
Zhang Y, Tan L, Yang Q, Li C, Liou Y. The microtubule-associated protein HURP recruits the centrosomal protein TACC3 to regulate K-fiber formation and support chromosome congression. J Biol Chem. 2018;293:15733-15747 pubmed publisher
  • western blot; human; loading ...; fig 4b
Kühnl A, Musiol A, Heitzig N, Johnson D, Ehrhardt C, Grewal T, et al. Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus. MBio. 2018;9: pubmed publisher
  • immunohistochemistry - frozen section; thale cress; 1:100; loading ...; fig 6c
Reipert S, Goldammer H, Richardson C, Goldberg M, Hawkins T, Hollergschwandtner E, et al. Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution. J Histochem Cytochem. 2018;66:903-921 pubmed publisher
  • western blot; human; loading ...; fig 1c
Bernal A, Moltó Abad M, Dominguez D, Tusell L. Acute telomere deprotection prevents ongoing BFB cycles and rampant instability in p16INK4a-deficient epithelial cells. Oncotarget. 2018;9:27151-27170 pubmed publisher
  • western blot; mouse; loading ...; fig 4f
Ribeiro de Almeida C, Dhir S, Dhir A, Moghaddam A, Sattentau Q, Meinhart A, et al. RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination. Mol Cell. 2018;70:650-662.e8 pubmed publisher
  • immunocytochemistry; human; 1:5000; loading ...; fig 1g
Bhardwaj A, Yang Y, Ueberheide B, Smith S. Whole proteome analysis of human tankyrase knockout cells reveals targets of tankyrase-mediated degradation. Nat Commun. 2017;8:2214 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4a
Stevenson N, Bergen D, Skinner R, Kague E, Martin Silverstone E, Robson Brown K, et al. Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression. J Cell Sci. 2017;130:4132-4143 pubmed publisher
  • western blot; human; loading ...; fig 8
Bartusch C, Döring T, Prange R. Rab33B Controls Hepatitis B Virus Assembly by Regulating Core Membrane Association and Nucleocapsid Processing. Viruses. 2017;9: pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig s5g
Moradi M, Sivadasan R, Saal L, Lüningschrör P, Dombert B, Rathod R, et al. Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons. J Cell Biol. 2017;216:793-814 pubmed publisher
  • immunocytochemistry; chicken; fig 10b
Boswell B, Korol A, West Mays J, Musil L. Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract. Mol Biol Cell. 2017;28:907-921 pubmed publisher
  • western blot; fruit fly ; loading ...; fig 1e
Vonk J, Yeshaw W, Pinto F, Faber A, Lahaye L, Kanon B, et al. Drosophila Vps13 Is Required for Protein Homeostasis in the Brain. PLoS ONE. 2017;12:e0170106 pubmed publisher
  • western blot; mouse; fig 6
  • western blot; human; fig 2
Cizmecioglu O, Ni J, Xie S, Zhao J, Roberts T. Rac1-mediated membrane raft localization of PI3K/p110? is required for its activation by GPCRs or PTEN loss. elife. 2016;5: pubmed publisher
  • western blot; Chinese hamsters; 1:5000; fig 1
  • western blot; human; 1:5000; fig 2
Qi L, Jafari N, Li X, Chen Z, Li L, Hytönen V, et al. Talin2-mediated traction force drives matrix degradation and cell invasion. J Cell Sci. 2016;129:3661-3674 pubmed
  • western blot; mouse; 1:5000; fig 4
Hu H, Umemori H, Hsueh Y. Postsynaptic SDC2 induces transsynaptic signaling via FGF22 for bidirectional synaptic formation. Sci Rep. 2016;6:33592 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Zhu S, Henninger K, McGrath B, Cavener D. PERK Regulates Working Memory and Protein Synthesis-Dependent Memory Flexibility. PLoS ONE. 2016;11:e0162766 pubmed publisher
  • western blot; human; 1:10,000; fig 1
Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher
  • western blot; human; fig 6
Agatheeswaran S, Pattnayak N, Chakraborty S. Identification and functional characterization of the miRNA-gene regulatory network in chronic myeloid leukemia lineage negative cells. Sci Rep. 2016;6:32493 pubmed publisher
  • western blot; fission yeast; 1:5000; fig 9
Rijal K, Maraia R. Active Center Control of Termination by RNA Polymerase III and tRNA Gene Transcription Levels In Vivo. PLoS Genet. 2016;12:e1006253 pubmed publisher
  • western blot; mouse; fig 7b
Ramo K, Sugamura K, Craige S, Keaney J, Davis R. Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development. elife. 2016;5: pubmed publisher
  • western blot; mouse; 1:10,000; fig 3
Lie E, Ko J, Choi S, Roh J, Cho Y, Noh R, et al. SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion. Nat Commun. 2016;7:12328 pubmed publisher
  • western blot; mouse; 1:2000; fig 2
Di Siena S, Gimmelli R, Nori S, Barbagallo F, Campolo F, Dolci S, et al. Activated c-Kit receptor in the heart promotes cardiac repair and regeneration after injury. Cell Death Dis. 2016;7:e2317 pubmed publisher
  • western blot; human; fig 4
Cao J, Wu L, Zhang S, Lu M, Cheung W, Cai W, et al. An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting. Nucleic Acids Res. 2016;44:e149 pubmed
  • western blot; human; 1:10,000; fig 5
Kurg R, Reinsalu O, Jagur S, Ounap K, Võsa L, Kasvandik S, et al. Biochemical and proteomic characterization of retrovirus Gag based microparticles carrying melanoma antigens. Sci Rep. 2016;6:29425 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
  • western blot; human; 1:200; fig s8
Yang C, Suzuki M, Yamakawa S, Uno S, Ishii A, Yamazaki S, et al. Claspin recruits Cdc7 kinase for initiation of DNA replication in human cells. Nat Commun. 2016;7:12135 pubmed publisher
  • immunocytochemistry; mouse; 1:2000; fig 10
  • western blot; mouse; 1:4000; fig 13
Schmitt D, Funk N, Blum R, Asan E, Andersen L, Rülicke T, et al. Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons. Histochem Cell Biol. 2016;146:489-512 pubmed publisher
  • western blot; mouse; fig 2
Marquer C, Tian H, Yi J, Bastien J, Dall Armi C, Yang Klingler Y, et al. Arf6 controls retromer traffic and intracellular cholesterol distribution via a phosphoinositide-based mechanism. Nat Commun. 2016;7:11919 pubmed publisher
  • western blot; mouse; fig 1
Toft D, Fuller M, Schipma M, Chen F, Cryns V, Layden B. ?B-crystallin and HspB2 deficiency is protective from diet-induced glucose intolerance. Genom Data. 2016;9:10-7 pubmed publisher
  • immunocytochemistry; mouse; 1:5000; loading ...; fig 5a
Dai J, Lu Y, Wang C, Chen X, Fan X, Gu H, et al. Vps33b regulates Vwf-positive vesicular trafficking in megakaryocytes. J Pathol. 2016;240:108-19 pubmed publisher
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 2
Skoge R, Ziegler M. SIRT2 inactivation reveals a subset of hyperacetylated perinuclear microtubules inaccessible to HDAC6. J Cell Sci. 2016;129:2972-82 pubmed publisher
  • western blot; human; 1:5000; fig 1
Qi Y, Yu J, Han W, Fan X, Qian H, Wei H, et al. A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation. Nat Commun. 2016;7:ncomms11840 pubmed publisher
  • immunocytochemistry; human; fig s4
  • western blot; human; fig 2
Brunk K, Zhu M, Bärenz F, Kratz A, Haselmann Weiss U, Antony C, et al. Cep78 is a new centriolar protein involved in Plk4-induced centriole overduplication. J Cell Sci. 2016;129:2713-8 pubmed publisher
  • western blot; fission yeast; fig 5
Ma N, Ma Y, Nakashima A, Kikkawa U, Furuyashiki T. The Loss of Lam2 and Npr2-Npr3 Diminishes the Vacuolar Localization of Gtr1-Gtr2 and Disinhibits TORC1 Activity in Fission Yeast. PLoS ONE. 2016;11:e0156239 pubmed publisher
  • western blot; human; 1:4000; fig 4
Lopez A, Kugelman J, Garcia Rivera J, Urias E, Salinas S, Fernandez Zapico M, et al. The Structure-Specific Recognition Protein 1 Associates with Lens Epithelium-Derived Growth Factor Proteins and Modulates HIV-1 Replication. J Mol Biol. 2016;428:2814-31 pubmed publisher
  • western blot; human; 1:1000; fig 1
Franks T, Benner C, Narvaiza I, Marchetto M, Young J, Malik H, et al. Evolution of a transcriptional regulator from a transmembrane nucleoporin. Genes Dev. 2016;30:1155-71 pubmed publisher
  • western blot; mouse; 1:10,000; fig s7
  • western blot; rat; 1:10,000; fig 3
Fusté N, Fernández Hernández R, Cemeli T, Mirantes C, Pedraza N, Rafel M, et al. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nat Commun. 2016;7:11581 pubmed publisher
  • western blot; human; fig 1
Lock R, Ingraham R, Maertens O, Miller A, Weledji N, Legius E, et al. Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers. J Clin Invest. 2016;126:2181-90 pubmed publisher
  • western blot; human; fig 1
Wang L, Lee K, Malonis R, SANCHEZ I, Dynlacht B. Tethering of an E3 ligase by PCM1 regulates the abundance of centrosomal KIAA0586/Talpid3 and promotes ciliogenesis. elife. 2016;5: pubmed publisher
  • western blot; human; fig 7
Maria O, Liu Y, El Hakim M, Zeitouni A, Tran S. The role of human fibronectin- or placenta basement membrane extract-based gels in favouring the formation of polarized salivary acinar-like structures. J Tissue Eng Regen Med. 2017;11:2643-2657 pubmed publisher
  • western blot; human; fig 1
Ahrabi S, Sarkar S, Pfister S, Pirovano G, Higgins G, Porter A, et al. A role for human homologous recombination factors in suppressing microhomology-mediated end joining. Nucleic Acids Res. 2016;44:5743-57 pubmed publisher
  • western blot; mouse; 1:25,000; fig 1
Rodriguez Ortiz C, Flores J, Valenzuela J, Rodriguez G, Zumkehr J, Tran D, et al. The Myoblast C2C12 Transfected with Mutant Valosin-Containing Protein Exhibits Delayed Stress Granule Resolution on Oxidative Stress. Am J Pathol. 2016;186:1623-34 pubmed publisher
  • western blot; fruit fly ; 1:10,000; fig 1
Kwenda L, Collins C, Dattoli A, Dunleavy E. Nucleolar activity and CENP-C regulate CENP-A and CAL1 availability for centromere assembly in meiosis. Development. 2016;143:1400-12 pubmed publisher
  • western blot; human; fig 1
Chaudhary S, Madhukrishna B, Adhya A, Keshari S, Mishra S. Overexpression of caspase 7 is ER? dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis. 2016;5:e219 pubmed publisher
  • western blot; Xenopus laevis; fig 2
Chu C, Ossipova O, Ioannou A, Sokol S. Prickle3 synergizes with Wtip to regulate basal body organization and cilia growth. Sci Rep. 2016;6:24104 pubmed publisher
  • western blot; human; 1:1000; fig 5
Canton J, Schlam D, Breuer C, Gutschow M, Glogauer M, Grinstein S. Calcium-sensing receptors signal constitutive macropinocytosis and facilitate the uptake of NOD2 ligands in macrophages. Nat Commun. 2016;7:11284 pubmed publisher
  • immunocytochemistry; mouse; fig 7
Yadav P, Selvaraj B, Bender F, Behringer M, Moradi M, Sivadasan R, et al. Neurofilament depletion improves microtubule dynamics via modulation of Stat3/stathmin signaling. Acta Neuropathol. 2016;132:93-110 pubmed publisher
  • immunocytochemistry; human; 1:3000; fig 5
  • western blot; human; 1:3000; fig 9
Eisch V, Lu X, Gabriel D, Djabali K. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget. 2016;7:24700-18 pubmed publisher
  • western blot; human; 1:5000; fig 1
Xu X, Tan X, Hulshoff M, Wilhelmi T, Zeisberg M, Zeisberg E. Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells. FEBS Lett. 2016;590:1222-33 pubmed publisher
  • western blot; human; 1:10,000; fig 2
Gschweitl M, Ulbricht A, Barnes C, Enchev R, Stoffel Studer I, Meyer Schaller N, et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. elife. 2016;5:e13841 pubmed publisher
  • western blot; human; 1:10,000; fig 2
Decorsière A, Mueller H, van Breugel P, Abdul F, Gerossier L, Beran R, et al. Hepatitis B virus X protein identifies the Smc5/6 complex as a host restriction factor. Nature. 2016;531:386-9 pubmed publisher
  • western blot; mouse; fig 5
Fong S, Lin H, Wu M, Chen C, Huang Y. CPEB3 Deficiency Elevates TRPV1 Expression in Dorsal Root Ganglia Neurons to Potentiate Thermosensation. PLoS ONE. 2016;11:e0148491 pubmed publisher
  • western blot; human; fig 7f
Ro S, Xue X, Ramakrishnan S, Cho C, Namkoong S, Jang I, et al. Tumor suppressive role of sestrin2 during colitis and colon carcinogenesis. elife. 2016;5:e12204 pubmed publisher
  • western blot; mouse; fig 2
  • western blot; human; fig 1
Ladurner R, Kreidl E, Ivanov M, Ekker H, Idarraga Amado M, Busslinger G, et al. Sororin actively maintains sister chromatid cohesion. EMBO J. 2016;35:635-53 pubmed publisher
  • western blot; human; fig s11
Sancho Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, et al. Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Nat Commun. 2016;7:10743 pubmed publisher
  • western blot; human; fig 1
Sieber J, Hauer C, Bhuvanagiri M, Leicht S, Krijgsveld J, Neu Yilik G, et al. Proteomic Analysis Reveals Branch-specific Regulation of the Unfolded Protein Response by Nonsense-mediated mRNA Decay. Mol Cell Proteomics. 2016;15:1584-97 pubmed publisher
  • western blot; human; fig 8
Gawron D, Ndah E, Gevaert K, Van Damme P. Positional proteomics reveals differences in N-terminal proteoform stability. Mol Syst Biol. 2016;12:858 pubmed publisher
  • western blot; mouse; 1:5000; fig 4b
Bowles J, Feng C, Miles K, Ineson J, Spiller C, Koopman P. ALDH1A1 provides a source of meiosis-inducing retinoic acid in mouse fetal ovaries. Nat Commun. 2016;7:10845 pubmed publisher
  • western blot; mouse; fig 3
Zhang Q, Gao X, Li C, Feliciano C, Wang D, Zhou D, et al. Impaired Dendritic Development and Memory in Sorbs2 Knock-Out Mice. J Neurosci. 2016;36:2247-60 pubmed publisher
  • western blot; hamsters; 1:10,000; fig 7
  • western blot; human; 1:10,000; fig 4
Prabhu A, Luu W, Sharpe L, Brown A. Cholesterol-mediated Degradation of 7-Dehydrocholesterol Reductase Switches the Balance from Cholesterol to Vitamin D Synthesis. J Biol Chem. 2016;291:8363-73 pubmed publisher
  • western blot; human
Hoffmann C, Mao X, Dieterle M, Moreau F, Al Absi A, Steinmetz A, et al. CRP2, a new invadopodia actin bundling factor critically promotes breast cancer cell invasion and metastasis. Oncotarget. 2016;7:13688-705 pubmed publisher
  • western blot; mouse; 1:3000; loading ...; fig 6f
  • western blot; human; 1:3000; loading ...; fig 5b
Alonso F, Domingos Pereira S, Le Gal L, Derré L, Meda P, Jichlinski P, et al. Targeting endothelial connexin40 inhibits tumor growth by reducing angiogenesis and improving vessel perfusion. Oncotarget. 2016;7:14015-28 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 9
Taverna E, Mora Bermúdez F, Strzyz P, Florio M, Icha J, Haffner C, et al. Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells. Sci Rep. 2016;6:21206 pubmed publisher
  • western blot; human; fig 1
Kim D, Helfman D. Loss of MLCK leads to disruption of cell-cell adhesion and invasive behavior of breast epithelial cells via increased expression of EGFR and ERK/JNK signaling. Oncogene. 2016;35:4495-508 pubmed publisher
  • western blot; mouse; fig 1
Khalid S, Drasche A, Thurner M, Hermann M, Ashraf M, Fresser F, et al. cJun N-terminal kinase (JNK) phosphorylation of serine 36 is critical for p66Shc activation. Sci Rep. 2016;6:20930 pubmed publisher
  • western blot; human; 1:4000; fig 1
Kim N, Kim M, Sung P, Bae Y, Shin E, Yoo J. Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A. Nat Commun. 2016;7:10631 pubmed publisher
  • western blot; mouse; fig 3
Liu C, Rajapakse A, Riedo E, Fellay B, Bernhard M, Montani J, et al. Targeting arginase-II protects mice from high-fat-diet-induced hepatic steatosis through suppression of macrophage inflammation. Sci Rep. 2016;6:20405 pubmed publisher
  • western blot; human; fig 6
Nagy Z, Kalousi A, Furst A, Koch M, Fischer B, Soutoglou E. Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs. PLoS Genet. 2016;12:e1005791 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Lancaster G, Kammoun H, Kraakman M, Kowalski G, Bruce C, Febbraio M. PKR is not obligatory for high-fat diet-induced obesity and its associated metabolic and inflammatory complications. Nat Commun. 2016;7:10626 pubmed publisher
  • western blot; mouse; 1:10,000; fig 7
Marmugi A, Parnis J, Chen X, Carmichael L, Hardy J, Mannan N, et al. Sorcin Links Pancreatic β-Cell Lipotoxicity to ER Ca2+ Stores. Diabetes. 2016;65:1009-21 pubmed publisher
  • immunocytochemistry; rat; fig 6i
Yau K, Schätzle P, Tortosa E, Pagès S, Holtmaat A, Kapitein L, et al. Dendrites In Vitro and In Vivo Contain Microtubules of Opposite Polarity and Axon Formation Correlates with Uniform Plus-End-Out Microtubule Orientation. J Neurosci. 2016;36:1071-85 pubmed publisher
  • western blot; human; 1:5000; fig 2
Tan X, Xu X, Zeisberg M, Zeisberg E. DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition. PLoS ONE. 2016;11:e0147816 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 2
  • western blot; mouse; 1:3000; fig 2
Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  • immunocytochemistry; chicken; fig 3
  • western blot; chicken; 1:2000; fig 2
Wood L, Booth D, Vargiu G, Ohta S, deLima Alves F, Samejima K, et al. Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability. Open Biol. 2016;6:150230 pubmed publisher
  • western blot; mouse; 1:1000; fig s1
Choi S, Chen Z, Tang L, Fang Y, Shin S, Panarelli N, et al. Bcl-xL promotes metastasis independent of its anti-apoptotic activity. Nat Commun. 2016;7:10384 pubmed publisher
  • western blot; mouse; fig 1
  • western blot; human; fig 1
Du Y, Yamaguchi H, Wei Y, Hsu J, Wang H, Hsu Y, et al. Blocking c-Met-mediated PARP1 phosphorylation enhances anti-tumor effects of PARP inhibitors. Nat Med. 2016;22:194-201 pubmed publisher
  • western blot; mouse; 1:25,000; fig s4c
Avgustinova A, Iravani M, Robertson D, Fearns A, Gao Q, Klingbeil P, et al. Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness. Nat Commun. 2016;7:10305 pubmed publisher
  • western blot; mouse; fig 1
Cherepkova M, Sineva G, Pospelov V. Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway. Cell Death Dis. 2016;7:e2050 pubmed publisher
  • western blot; mouse; fig s2
Quétier I, Marshall J, Spencer Dene B, Lachmann S, Casamassima A, Franco C, et al. Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion. Cell Rep. 2016;14:440-448 pubmed publisher
  • western blot; human; fig 3
Choi Y, Meghani K, Brault M, Leclerc L, He Y, Day T, et al. Platinum and PARP Inhibitor Resistance Due to Overexpression of MicroRNA-622 in BRCA1-Mutant Ovarian Cancer. Cell Rep. 2016;14:429-439 pubmed publisher
  • western blot; mouse; fig 1c
Yang Y, Li W, Hoque M, Hou L, Shen S, Tian B, et al. PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation. PLoS Genet. 2016;12:e1005794 pubmed publisher
  • western blot; human; 1:500
Ketel K, Krauss M, Nicot A, Puchkov D, Wieffer M, Müller R, et al. A phosphoinositide conversion mechanism for exit from endosomes. Nature. 2016;529:408-12 pubmed publisher
  • western blot; human; fig 2
Puvirajesinghe T, Bertucci F, Jain A, Scerbo P, Belotti E, Audebert S, et al. Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2-JNK signalling in breast cancer. Nat Commun. 2016;7:10318 pubmed publisher
  • western blot; mouse; 1:10,000; fig 2
Ruegsegger C, Stucki D, Steiner S, Angliker N, Radecke J, Keller E, et al. Impaired mTORC1-Dependent Expression of Homer-3 Influences SCA1 Pathophysiology. Neuron. 2016;89:129-46 pubmed publisher
  • western blot; human; fig 2
Wang M, Itoh M, Li S, Hida Y, Ohta K, Hayakawa M, et al. CED-4 is an mRNA-binding protein that delivers ced-3 mRNA to ribosomes. Biochem Biophys Res Commun. 2016;470:48-53 pubmed publisher
  • western blot; human; 1:8000; fig 4
Lee S, Frattini V, Bansal M, Castano A, Sherman D, Hutchinson K, et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016;529:172-7 pubmed publisher
  • western blot; mouse; 1:10,000; fig 1
Drusenheimer N, Migdal B, Jäckel S, Tveriakhina L, Scheider K, Schulz K, et al. The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling. PLoS Genet. 2015;11:e1005749 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 1
Gomez Villafuertes R, García Huerta P, Díaz Hernández J, Miras Portugal M. PI3K/Akt signaling pathway triggers P2X7 receptor expression as a pro-survival factor of neuroblastoma cells under limiting growth conditions. Sci Rep. 2015;5:18417 pubmed publisher
  • western blot; human; 1:4000; fig 5
Wille C, Nawandar D, Henning A, Ma S, Oetting K, Lee D, et al. 5-hydroxymethylation of the EBV genome regulates the latent to lytic switch. Proc Natl Acad Sci U S A. 2015;112:E7257-65 pubmed publisher
  • western blot; human; 1:1000; fig 1
Pillai S, Nguyen J, Johnson J, Haura E, Coppola D, Chellappan S. Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis. Nat Commun. 2015;6:10072 pubmed publisher
  • western blot; mouse; 1:5000; fig 3
Dobrowolny G, Bernardini C, Martini M, Baranzini M, Barba M, Musarò A. Muscle Expression of SOD1(G93A) Modulates microRNA and mRNA Transcription Pattern Associated with the Myelination Process in the Spinal Cord of Transgenic Mice. Front Cell Neurosci. 2015;9:463 pubmed publisher
  • western blot; zebrafish ; 1:10,000; fig 1
Veldman M, Rios Galdamez Y, Lu X, Gu X, Qin W, Li S, et al. The N17 domain mitigates nuclear toxicity in a novel zebrafish Huntington's disease model. Mol Neurodegener. 2015;10:67 pubmed publisher
  • western blot; human; fig 6
Lommel M, Trairatphisan P, Gäbler K, Laurini C, Muller A, Kaoma T, et al. L-plastin Ser5 phosphorylation in breast cancer cells and in vitro is mediated by RSK downstream of the ERK/MAPK pathway. FASEB J. 2016;30:1218-33 pubmed publisher
  • western blot; mouse; 1:5000; fig 6
Qian G, Fan W, Ahlemeyer B, Karnati S, Baumgart Vogt E. Peroxisomes in Different Skeletal Cell Types during Intramembranous and Endochondral Ossification and Their Regulation during Osteoblast Differentiation by Distinct Peroxisome Proliferator-Activated Receptors. PLoS ONE. 2015;10:e0143439 pubmed publisher
  • immunocytochemistry; human; fig 2
Qi D, Kaur Gill N, Santiskulvong C, Sifuentes J, Dorigo O, Rao J, et al. Screening cell mechanotype by parallel microfiltration. Sci Rep. 2015;5:17595 pubmed publisher
  • immunocytochemistry; human; 1:10,000; fig s2
Etemad B, Kuijt T, Kops G. Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint. Nat Commun. 2015;6:8987 pubmed publisher
  • western blot; mouse; fig 1
Fukuda M, Inoue M, Muramatsu D, Miyachi H, Shinkai Y. Knockout mouse production assisted by Blm knockdown. J Reprod Dev. 2016;62:121-5 pubmed publisher
  • western blot; human; 1:10,000; fig 2
Dewaele M, Tabaglio T, Willekens K, Bezzi M, Teo S, Low D, et al. Antisense oligonucleotide-mediated MDM4 exon 6 skipping impairs tumor growth. J Clin Invest. 2016;126:68-84 pubmed publisher
  • immunohistochemistry; soybeans; 1:800
Gzyl J, Chmielowska Bąk J, Przymusiński R, Gwóźdź E. Cadmium affects microtubule organization and post-translational modifications of tubulin in seedlings of soybean (Glycine max L.). Front Plant Sci. 2015;6:937 pubmed publisher
  • western blot; human; fig 1
Cristini A, Park J, Capranico G, Legube G, Favre G, Sordet O. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2016;44:1161-78 pubmed publisher
  • immunocytochemistry; mouse; fig 12
  • western blot; mouse; fig 12
Copeland S, Thurston S, Copeland J. Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1. Mol Biol Cell. 2016;27:260-76 pubmed publisher
  • western blot; human; 1:10,000; fig ev3
Fernández Santiago R, Carballo Carbajal I, Castellano G, Torrent R, Richaud Y, Sánchez Danés A, et al. Aberrant epigenome in iPSC-derived dopaminergic neurons from Parkinson's disease patients. EMBO Mol Med. 2015;7:1529-46 pubmed publisher
  • western blot; mouse; fig s4
Antonucci L, Fagman J, Kim J, Todoric J, Gukovsky I, Mackey M, et al. Basal autophagy maintains pancreatic acinar cell homeostasis and protein synthesis and prevents ER stress. Proc Natl Acad Sci U S A. 2015;112:E6166-74 pubmed publisher
  • western blot; human
Eiseler T, Wille C, Koehler C, Illing A, Seufferlein T. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem. 2016;291:462-77 pubmed publisher
  • immunocytochemistry; human; fig 2
Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, et al. A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis. PLoS Genet. 2015;11:e1005582 pubmed publisher
  • western blot; human; fig s7
Tonsing Carter E, Bailey B, Saadatzadeh M, Ding J, Wang H, Sinn A, et al. Potentiation of Carboplatin-Mediated DNA Damage by the Mdm2 Modulator Nutlin-3a in a Humanized Orthotopic Breast-to-Lung Metastatic Model. Mol Cancer Ther. 2015;14:2850-63 pubmed publisher
  • western blot; domestic sheep; 1:1000; fig 1a
Teng R, Wu T, Afolayan A, Konduri G. Nitrotyrosine impairs mitochondrial function in fetal lamb pulmonary artery endothelial cells. Am J Physiol Cell Physiol. 2016;310:C80-8 pubmed publisher
  • western blot; mouse; 1:50,000; fig 6
Bizet A, Becker Heck A, Ryan R, Weber K, Filhol E, Krug P, et al. Mutations in TRAF3IP1/IFT54 reveal a new role for IFT proteins in microtubule stabilization. Nat Commun. 2015;6:8666 pubmed publisher
  • western blot; human; 1:2000; fig 6
Müller W, Schröder H, Tolba E, Diehl Seifert B, Wang X. Mineralization of bone-related SaOS-2 cells under physiological hypoxic conditions. FEBS J. 2016;283:74-87 pubmed publisher
  • western blot; human; 1:1000; fig 2b
Klar A, Gopinadh J, Kleber S, Wadle A, Renner C. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing. PLoS ONE. 2015;10:e0139221 pubmed publisher
  • western blot; human; fig 2
Felli N, Errico M, Pedini F, Petrini M, Puglisi R, Bellenghi M, et al. AP2α controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression. Oncogene. 2016;35:3016-26 pubmed publisher
  • western blot; human; 1:2000; fig 3
Nawandar D, Wang A, Makielski K, Lee D, Ma S, Barlow E, et al. Differentiation-Dependent KLF4 Expression Promotes Lytic Epstein-Barr Virus Infection in Epithelial Cells. PLoS Pathog. 2015;11:e1005195 pubmed publisher
  • immunocytochemistry; human; fig 1
Voets E, Marsman J, Demmers J, Beijersbergen R, Wolthuis R. The lethal response to Cdk1 inhibition depends on sister chromatid alignment errors generated by KIF4 and isoform 1 of PRC1. Sci Rep. 2015;5:14798 pubmed publisher
  • western blot; human; fig 7a
Yokdang N, Hatakeyama J, Wald J, Simion C, Tellez J, Chang D, et al. LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells. Oncogene. 2016;35:2932-47 pubmed publisher
  • western blot; mouse; 1:1000
Basheer W, Harris B, Mentrup H, Abreha M, Thames E, Lea J, et al. Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis. J Mol Cell Cardiol. 2015;88:1-13 pubmed publisher
  • western blot; human; 1:2500; fig 2b
Ho D, Kim H, Kim J, Sim H, Ahn H, Kim J, et al. Leucine-Rich Repeat Kinase 2 (LRRK2) phosphorylates p53 and induces p21(WAF1/CIP1) expression. Mol Brain. 2015;8:54 pubmed publisher
  • western blot; human; 1:30,000; fig 3c
D Amato N, Rogers T, Gordon M, Greene L, Cochrane D, Spoelstra N, et al. A TDO2-AhR signaling axis facilitates anoikis resistance and metastasis in triple-negative breast cancer. Cancer Res. 2015;75:4651-64 pubmed publisher
  • western blot; human; fig 1
Zagani R, El Assaad W, Gamache I, Teodoro J. Inhibition of adipose triglyceride lipase (ATGL) by the putative tumor suppressor G0S2 or a small molecule inhibitor attenuates the growth of cancer cells. Oncotarget. 2015;6:28282-95 pubmed publisher
  • western blot; human; fig 1
Zhou N, Yuan S, Wang R, Zhang W, Chen J. Role of dual specificity tyrosine-phosphorylation-regulated kinase 1B (Dyrk1B) in S-phase entry of HPV E7 expressing cells from quiescence. Oncotarget. 2015;6:30745-61 pubmed publisher
  • western blot; human; 1:5000; fig 4
Kim S, Lee K, Choi J, Ringstad N, Dynlacht B. Nek2 activation of Kif24 ensures cilium disassembly during the cell cycle. Nat Commun. 2015;6:8087 pubmed publisher
  • western blot; mouse; fig 1g
Galicia Vázquez G, Chu J, Pelletier J. eIF4AII is dispensable for miRNA-mediated gene silencing. RNA. 2015;21:1826-33 pubmed publisher
  • western blot; human; 1:8000; fig 10a
Takata K, Tomida J, Reh S, Swanhart L, Takata M, Hukriede N, et al. Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN). J Biol Chem. 2015;290:24278-93 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 1
  • western blot; human; 1:1000; fig 2
Kanfer G, Courtheoux T, Peterka M, Meier S, Soste M, Melnik A, et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun. 2015;6:8015 pubmed publisher
  • western blot; human; fig 10
Genin M, Clement F, Fattaccioli A, Raes M, Michiels C. M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide. BMC Cancer. 2015;15:577 pubmed publisher
  • western blot; mouse; fig 1
Cymerman I, Gozdz A, Urbanska M, Milek J, Dziembowska M, Jaworski J. Structural Plasticity of Dendritic Spines Requires GSK3α and GSK3β. PLoS ONE. 2015;10:e0134018 pubmed publisher
  • western blot; human; 1:1000; fig s8
Sutani T, Sakata T, Nakato R, Masuda K, Ishibashi M, Yamashita D, et al. Condensin targets and reduces unwound DNA structures associated with transcription in mitotic chromosome condensation. Nat Commun. 2015;6:7815 pubmed publisher
  • western blot; human; fig 6
Navarro F, Lieberman J. miR-34 and p53: New Insights into a Complex Functional Relationship. PLoS ONE. 2015;10:e0132767 pubmed publisher
  • western blot; human; fig s6m
Li W, Hu Y, Oh S, Ma Q, Merkurjev D, Song X, et al. Condensin I and II Complexes License Full Estrogen Receptor α-Dependent Enhancer Activation. Mol Cell. 2015;59:188-202 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 3a
Rivas S, Armisén R, Rojas D, Maldonado E, Huerta H, Tapia J, et al. The Ski Protein is Involved in the Transformation Pathway of Aurora Kinase A. J Cell Biochem. 2016;117:334-43 pubmed publisher
  • immunocytochemistry; human; fig s1
Sadaie M, Dillon C, Narita M, Young A, Cairney C, Godwin L, et al. Cell-based screen for altered nuclear phenotypes reveals senescence progression in polyploid cells after Aurora kinase B inhibition. Mol Biol Cell. 2015;26:2971-85 pubmed publisher
  • western blot; mouse; fig 4b
Lebrun Julien F, Suter U. Combined HDAC1 and HDAC2 Depletion Promotes Retinal Ganglion Cell Survival After Injury Through Reduction of p53 Target Gene Expression. ASN Neuro. 2015;7: pubmed publisher
  • western blot; human
Madrigal Matute J, Fernandez García C, Blanco Colio L, Burillo E, Fortuño A, Martinez Pinna R, et al. Thioredoxin-1/peroxiredoxin-1 as sensors of oxidative stress mediated by NADPH oxidase activity in atherosclerosis. Free Radic Biol Med. 2015;86:352-61 pubmed publisher
  • western blot; human; fig 4
Macvicar T, Mannack L, Lees R, Lane J. Targeted siRNA Screens Identify ER-to-Mitochondrial Calcium Exchange in Autophagy and Mitophagy Responses in RPE1 Cells. Int J Mol Sci. 2015;16:13356-80 pubmed publisher
  • western blot; human
Galoian K, Qureshi A, D Ippolito G, Schiller P, Molinari M, Johnstone A, et al. Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1. Int J Oncol. 2015;47:465-72 pubmed publisher
  • western blot; human; fig 2
Heinemann A, Cullinane C, De Paoli Iseppi R, Wilmott J, Gunatilake D, Madore J, et al. Combining BET and HDAC inhibitors synergistically induces apoptosis of melanoma and suppresses AKT and YAP signaling. Oncotarget. 2015;6:21507-21 pubmed
  • western blot; human
Faggi F, Codenotti S, Poliani P, Cominelli M, Chiarelli N, Colombi M, et al. MURC/cavin-4 Is Co-Expressed with Caveolin-3 in Rhabdomyosarcoma Tumors and Its Silencing Prevents Myogenic Differentiation in the Human Embryonal RD Cell Line. PLoS ONE. 2015;10:e0130287 pubmed publisher
  • western blot; human; 1:5000; fig 2
Aspalter I, Gordon E, Dubrac A, Ragab A, Narloch J, Vizan P, et al. Alk1 and Alk5 inhibition by Nrp1 controls vascular sprouting downstream of Notch. Nat Commun. 2015;6:7264 pubmed publisher
  • western blot; mouse; 1:4000; fig 1
Tian L, Ding S, You Y, Li T, Liu Y, Wu X, et al. Leiomodin-3-deficient mice display nemaline myopathy with fast-myofiber atrophy. Dis Model Mech. 2015;8:635-41 pubmed publisher
  • western blot; human; 1:5000; fig 2
Moiseeva O, Lessard F, Acevedo Aquino M, Vernier M, Tsantrizos Y, Ferbeyre G. Mutant lamin A links prophase to a p53 independent senescence program. Cell Cycle. 2015;14:2408-21 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 4
Bosco D, Kenworthy R, Zorio D, Sang Q. Human mesenchymal stem cells are resistant to Paclitaxel by adopting a non-proliferative fibroblastic state. PLoS ONE. 2015;10:e0128511 pubmed publisher
  • western blot; mouse
Tojo Y, Sekine H, Hirano I, Pan X, Souma T, Tsujita T, et al. Hypoxia Signaling Cascade for Erythropoietin Production in Hepatocytes. Mol Cell Biol. 2015;35:2658-72 pubmed publisher
  • western blot; human
Yeh J, Kreimer S, Walker S, Emori M, Krystal H, Richardson A, et al. Granulin, a novel STAT3-interacting protein, enhances STAT3 transcriptional function and correlates with poorer prognosis in breast cancer. Genes Cancer. 2015;6:153-68 pubmed
  • western blot; human
Greenfeld H, Takasaki K, Walsh M, Ersing I, Bernhardt K, Ma Y, et al. TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. PLoS Pathog. 2015;11:e1004890 pubmed publisher
  • western blot; human; 1:50,000; fig 7
Heubach J, Monsior J, Deenen R, Niegisch G, Szarvas T, Niedworok C, et al. The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells. Mol Cancer. 2015;14:108 pubmed publisher
  • western blot; human
De Zio D, Molinari F, Rizza S, Gatta L, Ciotti M, Salvatore A, et al. Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. Cell Mol Life Sci. 2015;72:4173-91 pubmed publisher
  • western blot; mouse; fig 2
Ketema M, Secades P, Kreft M, Nahidiazar L, Janssen H, Jalink K, et al. The rod domain is not essential for the function of plectin in maintaining tissue integrity. Mol Biol Cell. 2015;26:2402-17 pubmed publisher
  • western blot; human; 1:10,000; fig 1
Prosser S, Sahota N, Pelletier L, Morrison C, Fry A. Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosis. J Cell Biol. 2015;209:339-48 pubmed publisher
  • western blot; human
Bergeron D, Pal G, Beaulieu Y, Chabot B, Bachand F. Regulated Intron Retention and Nuclear Pre-mRNA Decay Contribute to PABPN1 Autoregulation. Mol Cell Biol. 2015;35:2503-17 pubmed publisher
  • immunocytochemistry; dogs; fig 1
  • western blot; dogs; fig 1
  • immunocytochemistry; mouse; fig 1
  • western blot; mouse; fig 1
Castelli M, De Pascalis C, Distefano G, Ducano N, Oldani A, Lanzetti L, et al. Regulation of the microtubular cytoskeleton by Polycystin-1 favors focal adhesions turnover to modulate cell adhesion and migration. BMC Cell Biol. 2015;16:15 pubmed publisher
  • western blot; mouse; 1:200,000; fig 2
Liu P, Paulson J, Forster C, Shapiro S, Ashe K, Zahs K. Characterization of a Novel Mouse Model of Alzheimer's Disease--Amyloid Pathology and Unique β-Amyloid Oligomer Profile. PLoS ONE. 2015;10:e0126317 pubmed publisher
  • western blot; human; fig s3
Naipal K, Raams A, Bruens S, Brandsma I, Verkaik N, Jaspers N, et al. Attenuated XPC expression is not associated with impaired DNA repair in bladder cancer. PLoS ONE. 2015;10:e0126029 pubmed publisher
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
Isogai T, van der Kammen R, Innocenti M. SMIFH2 has effects on Formins and p53 that perturb the cell cytoskeleton. Sci Rep. 2015;5:9802 pubmed publisher
  • western blot; human; fig 3
Mishra D, Chaudhary S, Krishna B, Mishra S. Identification of Critical Elements for Regulation of Inorganic Pyrophosphatase (PPA1) in MCF7 Breast Cancer Cells. PLoS ONE. 2015;10:e0124864 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, et al. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis. PLoS ONE. 2015;10:e0124213 pubmed publisher
  • western blot; human; fig 1
Sun Y, Ponz Sarvisé M, Chang S, Chang W, Chen C, Hsu J, et al. Proteasome inhibition enhances the killing effect of BikDD gene therapy. Am J Transl Res. 2015;7:319-27 pubmed
  • western blot; human
Pozo K, Hillmann A, Augustyn A, Plattner F, Hai T, Singh T, et al. Differential expression of cell cycle regulators in CDK5-dependent medullary thyroid carcinoma tumorigenesis. Oncotarget. 2015;6:12080-93 pubmed
  • western blot; human; 1:5000; fig 1
Khuperkar D, Helen M, Magre I, Joseph J. Inter-cellular transport of ran GTPase. PLoS ONE. 2015;10:e0125506 pubmed publisher
  • western blot; mouse; fig 5a
Brzoska E, Kowalski K, Markowska Zagrajek A, Kowalewska M, Archacki R, Plaskota I, et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther. 2015;6:46 pubmed publisher
  • western blot; mouse; 1:2000; fig 4
Lu J, Luo C, Bali K, Xie R, Mains R, Eipper B, et al. A role for Kalirin-7 in nociceptive sensitization via activity-dependent modulation of spinal synapses. Nat Commun. 2015;6:6820 pubmed publisher
  • western blot; human
Antonenkov V, Isomursu A, Mennerich D, Vapola M, Weiher H, Kietzmann T, et al. The Human Mitochondrial DNA Depletion Syndrome Gene MPV17 Encodes a Non-selective Channel That Modulates Membrane Potential. J Biol Chem. 2015;290:13840-61 pubmed publisher
  • western blot; human; fig 3
Ohashi M, Holthaus A, Calderwood M, Lai C, Krastins B, Sarracino D, et al. The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth. PLoS Pathog. 2015;11:e1004822 pubmed publisher
  • western blot; human; fig s6
Westcott J, Prechtl A, Maine E, Dang T, Esparza M, Sun H, et al. An epigenetically distinct breast cancer cell subpopulation promotes collective invasion. J Clin Invest. 2015;125:1927-43 pubmed publisher
  • immunohistochemistry; human; fig 6
Bazellières E, Conte V, Elosegui Artola A, Serra Picamal X, Bintanel Morcillo M, Roca Cusachs P, et al. Control of cell-cell forces and collective cell dynamics by the intercellular adhesome. Nat Cell Biol. 2015;17:409-20 pubmed publisher
  • western blot; mouse
Perdigão Henriques R, Petrocca F, Altschuler G, Thomas M, Le M, Tan S, et al. miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Oncogene. 2016;35:158-72 pubmed publisher
  • western blot; mouse; 1:2000
Philippou A, Minozzo F, Spinazzola J, Smith L, Lei H, Rassier D, et al. Masticatory muscles of mouse do not undergo atrophy in space. FASEB J. 2015;29:2769-79 pubmed publisher
  • western blot; mouse; 1:5000; fig 3e
Han J, Song B, Kim J, Kodali V, Pottekat A, Wang M, et al. Antioxidants Complement the Requirement for Protein Chaperone Function to Maintain β-Cell Function and Glucose Homeostasis. Diabetes. 2015;64:2892-904 pubmed publisher
  • western blot; human; 1:30000
Leclere L, Fransolet M, Côté F, Cambier P, Arnould T, Van Cutsem P, et al. Heat-modified citrus pectin induces apoptosis-like cell death and autophagy in HepG2 and A549 cancer cells. PLoS ONE. 2015;10:e0115831 pubmed publisher
  • western blot; mouse; 1:5000; fig 1
Koh H, Chang C, Jeon S, Yoon H, Ahn Y, Kim H, et al. The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia. Nat Commun. 2015;6:6340 pubmed publisher
  • western blot; human; 1:10000
Prosser S, Morrison C. Centrin2 regulates CP110 removal in primary cilium formation. J Cell Biol. 2015;208:693-701 pubmed publisher
  • immunoprecipitation; human; fig s3
Aguilar Arnal L, Katada S, Orozco Solis R, Sassone Corsi P. NAD(+)-SIRT1 control of H3K4 trimethylation through circadian deacetylation of MLL1. Nat Struct Mol Biol. 2015;22:312-8 pubmed publisher
  • immunocytochemistry; human
Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  • immunohistochemistry; human; 1:3000; fig 3
Iemura K, Tanaka K. Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules. Nat Commun. 2015;6:6447 pubmed publisher
  • western blot; human
Martínez Torres A, Quiney C, Attout T, Boullet H, Herbi L, Vela L, et al. CD47 agonist peptides induce programmed cell death in refractory chronic lymphocytic leukemia B cells via PLCγ1 activation: evidence from mice and humans. PLoS Med. 2015;12:e1001796 pubmed publisher
  • reverse phase protein lysate microarray; human; 1:5000; fig 5
Thangavel S, Berti M, Levikova M, Pinto C, Gomathinayagam S, Vujanovic M, et al. DNA2 drives processing and restart of reversed replication forks in human cells. J Cell Biol. 2015;208:545-62 pubmed publisher
  • western blot; mouse
Xiong J, Todorova D, Su N, Kim J, Lee P, Shen Z, et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. J Cell Biol. 2015;208:513-20 pubmed publisher
  • western blot; human; fig 2
Kratz A, Bärenz F, Richter K, Hoffmann I. Plk4-dependent phosphorylation of STIL is required for centriole duplication. Biol Open. 2015;4:370-7 pubmed publisher
  • western blot; human
Wang Y, Tan B, Mu R, Chang Y, Wu M, Tu H, et al. Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor κB (NF-κB) signaling. J Biol Chem. 2015;290:10395-405 pubmed publisher
  • immunocytochemistry; human; 1:2000; loading ...; fig 1a
Ohta S, Wood L, Toramoto I, Yagyu K, Fukagawa T, Earnshaw W. CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly. Mol Biol Cell. 2015;26:1225-37 pubmed publisher
  • western blot; human; fig 5
Guerrero A, Iglesias C, Raguz S, Floridia E, Gil J, Pombo C, et al. The cerebral cavernous malformation 3 gene is necessary for senescence induction. Aging Cell. 2015;14:274-83 pubmed publisher
  • western blot; human
González N, Martín Duce A, Martínez Arrieta F, Moreno Villegas Z, Portal Núñez S, Sanz R, et al. Effect of bombesin receptor subtype-3 and its synthetic agonist on signaling, glucose transport and metabolism in myocytes from patients with obesity and type 2 diabetes. Int J Mol Med. 2015;35:925-31 pubmed publisher
  • western blot; human
Michel S, Canonne M, Arnould T, Renard P. Inhibition of mitochondrial genome expression triggers the activation of CHOP-10 by a cell signaling dependent on the integrated stress response but not the mitochondrial unfolded protein response. Mitochondrion. 2015;21:58-68 pubmed publisher
  • western blot; human
Kozlova N, Samoylenko A, Drobot L, Kietzmann T. Urokinase is a negative modulator of Egf-dependent proliferation and motility in the two breast cancer cell lines MCF-7 and MDA-MB-231. Mol Carcinog. 2016;55:170-81 pubmed publisher
  • western blot; human; fig 3
Kwun H, Shuda M, Camacho C, Gamper A, Thant M, Chang Y, et al. Restricted protein phosphatase 2A targeting by Merkel cell polyomavirus small T antigen. J Virol. 2015;89:4191-200 pubmed publisher
  • western blot; mouse; 1:8000; fig 2
Piatti P, Lim C, Nat R, Villunger A, Geley S, Shue Y, et al. Embryonic stem cell differentiation requires full length Chd1. Sci Rep. 2015;5:8007 pubmed publisher
  • immunocytochemistry; human; fig s4
  • western blot; human; fig 1
Bele A, Mirza S, Zhang Y, Ahmad Mir R, Lin S, Kim J, et al. The cell cycle regulator ecdysoneless cooperates with H-Ras to promote oncogenic transformation of human mammary epithelial cells. Cell Cycle. 2015;14:990-1000 pubmed publisher
  • western blot; human; 1:20,000; fig 1
Niemeyer B, Parrish J, Spoelstra N, Joyal T, Richer J, Jedlicka P. Variable expression of PIK3R3 and PTEN in Ewing Sarcoma impacts oncogenic phenotypes. PLoS ONE. 2015;10:e0116895 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 4B
  • western blot; human; 1:1000; fig 1A
Silva V, Plooster M, Leung J, Cassimeris L. A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells. Cell Cycle. 2015;14:1070-81 pubmed publisher
  • immunocytochemistry; rhesus macaque; fig 2
van Bergeijk P, Adrian M, Hoogenraad C, Kapitein L. Optogenetic control of organelle transport and positioning. Nature. 2015;518:111-114 pubmed publisher
  • western blot; human; 1:5000; fig 6
Renard H, Simunovic M, Lemière J, Boucrot E, Garcia Castillo M, Arumugam S, et al. Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis. Nature. 2015;517:493-6 pubmed publisher
  • western blot; mouse; 1:4000
Katsunuma K, Yoshinaga K, Ohira Y, Eta R, Sato T, Horii T, et al. Z-100, extracted from Mycobacterium tuberculosis strain Aoyama B, promotes TNF-α production via nucleotide-binding oligomerization domain containing 2 (Nod2)-dependent NF-κB activation in RAW264.7 cells. Mol Immunol. 2015;64:218-27 pubmed publisher
  • western blot; mouse; fig 2
Shen W, Li H, Chen G, Chern Y, Tu P. Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism. Autophagy. 2015;11:685-700 pubmed publisher
  • western blot; human; fig 3
Diesenberg K, Beerbaum M, Fink U, Schmieder P, Krauss M. SEPT9 negatively regulates ubiquitin-dependent downregulation of EGFR. J Cell Sci. 2015;128:397-407 pubmed publisher
  • western blot; mouse; 1:8000; fig 1
Meng L, Ward A, Chun S, Bennett C, Beaudet A, Rigo F. Towards a therapy for Angelman syndrome by targeting a long non-coding RNA. Nature. 2015;518:409-12 pubmed publisher
  • western blot; human; 1:10000; fig s1
Nijenhuis W, Vallardi G, Teixeira A, Kops G, Saurin A. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 2014;16:1257-64 pubmed publisher
  • western blot; mouse
  • western blot; human
Chen J, Shin J, Zhao R, Phan L, Wang H, Xue Y, et al. CSN6 drives carcinogenesis by positively regulating Myc stability. Nat Commun. 2014;5:5384 pubmed publisher
  • western blot; human; fig 2
Komaravolu R, Adam C, Moonen J, Harmsen M, Goebeler M, Schmidt M. Erk5 inhibits endothelial migration via KLF2-dependent down-regulation of PAK1. Cardiovasc Res. 2015;105:86-95 pubmed publisher
  • western blot; mouse; 1:4000; fig f7s2
Katz Y, Li F, Lambert N, Sokol E, Tam W, Cheng A, et al. Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state. elife. 2014;3:e03915 pubmed publisher
  • western blot; fruit fly ; 1:2000
Carmena M, Lombardía M, Ogawa H, Earnshaw W. Polo kinase regulates the localization and activity of the chromosomal passenger complex in meiosis and mitosis in Drosophila melanogaster. Open Biol. 2014;4:140162 pubmed publisher
  • western blot; human
Kim K, Ossipova O, Sokol S. Neural crest specification by inhibition of the ROCK/Myosin II pathway. Stem Cells. 2015;33:674-85 pubmed publisher
  • immunocytochemistry; human; 1:500
  • immunohistochemistry - paraffin section; mouse; 1:5000
Sun Q, Luo T, Ren Y, Florey O, Shirasawa S, Sasazuki T, et al. Competition between human cells by entosis. Cell Res. 2014;24:1299-310 pubmed publisher
  • western blot; human
Nishibuchi G, Machida S, Osakabe A, Murakoshi H, Hiragami Hamada K, Nakagawa R, et al. N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity. Nucleic Acids Res. 2014;42:12498-511 pubmed publisher
  • western blot; mouse; fig 2
  • western blot; human; fig 5
Van der Meulen J, Sanghvi V, Mavrakis K, Durinck K, Fang F, Matthijssens F, et al. The H3K27me3 demethylase UTX is a gender-specific tumor suppressor in T-cell acute lymphoblastic leukemia. Blood. 2015;125:13-21 pubmed publisher
  • western blot; human; 1:4000
Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele J, et al. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J Extracell Vesicles. 2014;3: pubmed publisher
  • western blot; mouse; fig 4
Liang N, Zhang C, Dill P, Panasyuk G, Pion D, Koka V, et al. Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex. J Exp Med. 2014;211:2249-63 pubmed publisher
  • western blot; mouse; fig 1
Charlaftis N, Suddason T, Wu X, Anwar S, Karin M, Gallagher E. The MEKK1 PHD ubiquitinates TAB1 to activate MAPKs in response to cytokines. EMBO J. 2014;33:2581-96 pubmed publisher
  • western blot; mouse
Gelfand M, Hagan N, Tata A, Oh W, Lacoste B, Kang K, et al. Neuropilin-1 functions as a VEGFR2 co-receptor to guide developmental angiogenesis independent of ligand binding. elife. 2014;3:e03720 pubmed publisher
  • immunocytochemistry; human
Wrzesinski K, Rogowska Wrzesinska A, Kanlaya R, Borkowski K, Schwämmle V, Dai J, et al. The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments. PLoS ONE. 2014;9:e106973 pubmed publisher
  • immunocytochemistry; human; fig 2
Yanagi T, Krajewska M, Matsuzawa S, Reed J. PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells. Cancer Res. 2014;74:5795-807 pubmed publisher
  • western blot; mouse
Saini Y, Dang H, Livraghi Butrico A, Kelly E, Jones L, O Neal W, et al. Gene expression in whole lung and pulmonary macrophages reflects the dynamic pathology associated with airway surface dehydration. BMC Genomics. 2014;15:726 pubmed publisher
  • immunocytochemistry; human; 1:1000
Rharass T, Lemcke H, Lantow M, Kuznetsov S, Weiss D, Panàkovà D. Ca2+-mediated mitochondrial reactive oxygen species metabolism augments Wnt/?-catenin pathway activation to facilitate cell differentiation. J Biol Chem. 2014;289:27937-51 pubmed publisher
  • western blot; human
Bazot Q, Deschamps T, Tafforeau L, Siouda M, Leblanc P, Harth Hertle M, et al. Epstein-Barr virus nuclear antigen 3A protein regulates CDKN2B transcription via interaction with MIZ-1. Nucleic Acids Res. 2014;42:9700-16 pubmed publisher
  • western blot; human
  • western blot; mouse
Vassilopoulos A, Tominaga Y, Kim H, Lahusen T, Li B, Yu H, et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2015;34:3023-35 pubmed publisher
  • immunocytochemistry; human; 1:100
Silva P, Reis R, Bolanos Garcia V, Florindo C, Tavares A, Bousbaa H. Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles. FEBS Lett. 2014;588:3265-73 pubmed publisher
  • western blot; mouse; 1:2000
Requejo Aguilar R, Lopez Fabuel I, Fernandez E, Martins L, Almeida A, Bolanos J. PINK1 deficiency sustains cell proliferation by reprogramming glucose metabolism through HIF1. Nat Commun. 2014;5:4514 pubmed publisher
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse
  • western blot; mouse
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Ro S, Semple I, Park H, Park H, Park H, Kim M, et al. Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of p62/sequestosome-1. FEBS J. 2014;281:3816-27 pubmed publisher
  • western blot; mouse; 1:20,000; fig 3
Moorwood C, Philippou A, Spinazzola J, Keyser B, Macarak E, Barton E. Absence of ?-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle. Skelet Muscle. 2014;4:13 pubmed publisher
  • western blot; human
Mungamuri S, Wang S, Manfredi J, Gu W, Aaronson S. Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene. 2015;34:2461-70 pubmed publisher
  • western blot; human; 1:5000
Chondrogiannis G, Kastamoulas M, Kanavaros P, Vartholomatos G, Bai M, Baltogiannis D, et al. Cytokine effects on cell viability and death of prostate carcinoma cells. Biomed Res Int. 2014;2014:536049 pubmed publisher
  • western blot; rat
Fernandes J, Vieira M, Carreto L, Santos M, Duarte C, Carvalho A, et al. In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons. PLoS ONE. 2014;9:e99958 pubmed publisher
  • immunocytochemistry; human; 1:200
  • immunocytochemistry; dogs; 1:200
  • western blot; dogs; 1:1000; fig 7
Glotfelty L, Zahs A, Hodges K, Shan K, Alto N, Hecht G. Enteropathogenic E. coli effectors EspG1/G2 disrupt microtubules, contribute to tight junction perturbation and inhibit restoration. Cell Microbiol. 2014;16:1767-83 pubmed publisher
  • immunohistochemistry; human; fig 3
Antanavičiūtė I, Rysevaite K, Liutkevicius V, Marandykina A, Rimkute L, Sveikatiene R, et al. Long-distance communication between laryngeal carcinoma cells. PLoS ONE. 2014;9:e99196 pubmed publisher
  • western blot; human; 1:1000
Qiu W, Kassem M. miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation. Biochim Biophys Acta. 2014;1843:2114-21 pubmed publisher
  • western blot; mouse
Mamouni K, Cristini A, Guirouilh Barbat J, Monferran S, Lemarie A, Faye J, et al. RhoB promotes ?H2AX dephosphorylation and DNA double-strand break repair. Mol Cell Biol. 2014;34:3144-55 pubmed publisher
  • western blot; mouse
Premkumar M, Sule G, Nagamani S, Chakkalakal S, Nordin A, Jain M, et al. Argininosuccinate lyase in enterocytes protects from development of necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol. 2014;307:G347-54 pubmed publisher
  • western blot; human
Sung P, Murayama A, Kang W, Kim M, Yoon S, Fukasawa M, et al. Hepatitis C virus entry is impaired by claudin-1 downregulation in diacylglycerol acyltransferase-1-deficient cells. J Virol. 2014;88:9233-44 pubmed publisher
  • western blot; mouse; 1:1000
Fernandes K, Harder J, JOHN S, Shrager P, Libby R. DLK-dependent signaling is important for somal but not axonal degeneration of retinal ganglion cells following axonal injury. Neurobiol Dis. 2014;69:108-16 pubmed publisher
  • immunocytochemistry; human
Ling Y, Wong C, Li K, Chan K, Boukamp P, Liu W. CCHCR1 interacts with EDC4, suggesting its localization in P-bodies. Exp Cell Res. 2014;327:12-23 pubmed publisher
  • western blot; mouse
Godde N, Sheridan J, Smith L, Pearson H, Britt K, Galea R, et al. Scribble modulates the MAPK/Fra1 pathway to disrupt luminal and ductal integrity and suppress tumour formation in the mammary gland. PLoS Genet. 2014;10:e1004323 pubmed publisher
  • western blot; human
Choi Y, Pan Y, Park E, Konstantinopoulos P, De S, D ANDREA A, et al. MicroRNAs down-regulate homologous recombination in the G1 phase of cycling cells to maintain genomic stability. elife. 2014;3:e02445 pubmed publisher
  • western blot; human; 1:10000
Hans F, Fiesel F, Strong J, J ckel S, Rasse T, Geisler S, et al. UBE2E ubiquitin-conjugating enzymes and ubiquitin isopeptidase Y regulate TDP-43 protein ubiquitination. J Biol Chem. 2014;289:19164-79 pubmed publisher
  • western blot; human
Barrios A, Gómez A, Sáez J, Ciossani G, Toffolo E, Battaglioli E, et al. Differential properties of transcriptional complexes formed by the CoREST family. Mol Cell Biol. 2014;34:2760-70 pubmed
  • western blot; human; 1:5000
Zhu Y, Liu C, Cui Y, Nadiminty N, Lou W, Gao A. Interleukin-6 induces neuroendocrine differentiation (NED) through suppression of RE-1 silencing transcription factor (REST). Prostate. 2014;74:1086-94 pubmed publisher
  • western blot; human; 1:5000
Ma L, Aslanian A, Sun H, Jin M, Shi Y, Yates J, et al. Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system. Mol Cell Proteomics. 2014;13:1659-75 pubmed publisher
  • western blot; Saccharomycetales
  • western blot; fission yeast
Okada N, Toda T, Yamamoto M, Sato M. CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly. Mol Biol Cell. 2014;25:1969-82 pubmed publisher
  • western blot; human
Negishi M, Wongpalee S, Sarkar S, Park J, Lee K, Shibata Y, et al. A new lncRNA, APTR, associates with and represses the CDKN1A/p21 promoter by recruiting polycomb proteins. PLoS ONE. 2014;9:e95216 pubmed publisher
  • western blot; human; 1:5000
Rydell G, Renard H, Garcia Castillo M, Dingli F, Loew D, Lamaze C, et al. Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport. Traffic. 2014;15:772-87 pubmed publisher
  • western blot; human
Kuijt T, Omerzu M, Saurin A, Kops G. Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Chromosoma. 2014;123:471-80 pubmed publisher
  • immunocytochemistry; mouse; 1:500
Daems C, Martin L, Brousseau C, Tremblay J. MEF2 is restricted to the male gonad and regulates expression of the orphan nuclear receptor NR4A1. Mol Endocrinol. 2014;28:886-98 pubmed publisher
  • western blot; mouse
Sutherland J, Fraser B, Sobinoff A, Pye V, Davidson T, Siddall N, et al. Developmental expression of Musashi-1 and Musashi-2 RNA-binding proteins during spermatogenesis: analysis of the deleterious effects of dysregulated expression. Biol Reprod. 2014;90:92 pubmed publisher
  • western blot; mouse; fig 1
Dubois V, Laurent M, Sinnesael M, Cielen N, Helsen C, Clinckemalie L, et al. A satellite cell-specific knockout of the androgen receptor reveals myostatin as a direct androgen target in skeletal muscle. FASEB J. 2014;28:2979-94 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Turgay Y, Champion L, Balázs C, Held M, Toso A, Gerlich D, et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. J Cell Biol. 2014;204:1099-109 pubmed publisher
  • western blot; human
Waitkus M, Chandrasekharan U, Willard B, Tee T, Hsieh J, Przybycin C, et al. Signal integration and gene induction by a functionally distinct STAT3 phosphoform. Mol Cell Biol. 2014;34:1800-11 pubmed publisher
  • western blot; mouse; fig 6
  • western blot; human; fig 6
Meehan A, Saenz D, Guevera R, Morrison J, Peretz M, Fadel H, et al. A cyclophilin homology domain-independent role for Nup358 in HIV-1 infection. PLoS Pathog. 2014;10:e1003969 pubmed publisher
  • western blot; human
Khan K, Schneider Poetsch T, Ishfaq M, Ito A, Yoshimoto R, Mukaida N, et al. Splicing inhibition induces gene expression through canonical NF-?B pathway and extracellular signal-related kinase activation. FEBS Lett. 2014;588:1053-7 pubmed publisher
  • western blot; human
Gonçalves V, Henriques A, Henriques A, Pereira J, Pereira J, Neves Costa A, et al. Phosphorylation of SRSF1 by SRPK1 regulates alternative splicing of tumor-related Rac1b in colorectal cells. RNA. 2014;20:474-82 pubmed publisher
  • western blot; mouse
Wang Y, Kuramitsu Y, Tokuda K, Okada F, Baron B, Akada J, et al. Proteomic analysis indicates that overexpression and nuclear translocation of lactoylglutathione lyase (GLO1) is associated with tumor progression in murine fibrosarcoma. Electrophoresis. 2014;35:2195-202 pubmed publisher
  • western blot; human
Martínez Diez M, Guillén Navarro M, Pera B, Bouchet B, Martinez Leal J, Barasoain I, et al. PM060184, a new tubulin binding agent with potent antitumor activity including P-glycoprotein over-expressing tumors. Biochem Pharmacol. 2014;88:291-302 pubmed publisher
  • western blot; human
Cheng Y, Holloway M, Nguyen K, McCauley D, Landesman Y, Kauffman M, et al. XPO1 (CRM1) inhibition represses STAT3 activation to drive a survivin-dependent oncogenic switch in triple-negative breast cancer. Mol Cancer Ther. 2014;13:675-86 pubmed publisher
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
Potthoff E, Franco D, D Alessandro V, Starck C, Falk V, Zambelli T, et al. Toward a rational design of surface textures promoting endothelialization. Nano Lett. 2014;14:1069-79 pubmed publisher
  • western blot; mouse; fig 1, 2
Ashraf M, Ebner M, Wallner C, Haller M, Khalid S, Schwelberger H, et al. A p38MAPK/MK2 signaling pathway leading to redox stress, cell death and ischemia/reperfusion injury. Cell Commun Signal. 2014;12:6 pubmed publisher
  • immunocytochemistry; human
Diagouraga B, Grichine A, Fertin A, Wang J, Khochbin S, Sadoul K. Motor-driven marginal band coiling promotes cell shape change during platelet activation. J Cell Biol. 2014;204:177-85 pubmed publisher
  • western blot; mouse; fig 5, 7
Zemljic Harpf A, Godoy J, Platoshyn O, Asfaw E, Busija A, Domenighetti A, et al. Vinculin directly binds zonula occludens-1 and is essential for stabilizing connexin-43-containing gap junctions in cardiac myocytes. J Cell Sci. 2014;127:1104-16 pubmed publisher
  • western blot; mouse; 1:500
Honarpour N, Rose C, Brumbaugh J, Anderson J, Graham R, Sweredoski M, et al. F-box protein FBXL16 binds PP2A-B55? and regulates differentiation of embryonic stem cells along the FLK1+ lineage. Mol Cell Proteomics. 2014;13:780-91 pubmed publisher
  • western blot; human
D Anselmi F, Masiello M, Cucina A, Proietti S, Dinicola S, Pasqualato A, et al. Microenvironment promotes tumor cell reprogramming in human breast cancer cell lines. PLoS ONE. 2013;8:e83770 pubmed publisher
  • western blot; human
Miura S, Hamada S, Masamune A, Satoh K, Shimosegawa T. CUB-domain containing protein 1 represses the epithelial phenotype of pancreatic cancer cells. Exp Cell Res. 2014;321:209-18 pubmed publisher
  • western blot; human
Chen Y, Thang M, Chan Y, Huang Y, Ma N, Yu A, et al. Global assessment of Antrodia cinnamomea-induced microRNA alterations in hepatocarcinoma cells. PLoS ONE. 2013;8:e82751 pubmed publisher
  • western blot; Xenopus laevis
Chu C, Gerstenzang E, Ossipova O, Sokol S. Lulu regulates Shroom-induced apical constriction during neural tube closure. PLoS ONE. 2013;8:e81854 pubmed publisher
  • western blot; fission yeast; 1:30000
Rødland G, Tvegård T, Boye E, Grallert B. Crosstalk between the Tor and Gcn2 pathways in response to different stresses. Cell Cycle. 2014;13:453-61 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Liu X, Xiao W, Wang X, Li Y, Han J, Li Y. The p38-interacting protein (p38IP) regulates G2/M progression by promoting ?-tubulin acetylation via inhibiting ubiquitination-induced degradation of the acetyltransferase GCN5. J Biol Chem. 2013;288:36648-61 pubmed publisher
  • immunocytochemistry; human
Singh A, Zapata M, Choi Y, Yoon S. GSI promotes vincristine-induced apoptosis by enhancing multi-polar spindle formation. Cell Cycle. 2014;13:157-66 pubmed publisher
  • western blot; human; fig 2
de Poot S, Lai K, van der Wal L, Plasman K, Van Damme P, Porter A, et al. Granzyme M targets topoisomerase II alpha to trigger cell cycle arrest and caspase-dependent apoptosis. Cell Death Differ. 2014;21:416-26 pubmed publisher
  • western blot; human
Eldin P, Chazal N, Fenard D, Bernard E, Guichou J, Briant L. Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA. Nucleic Acids Res. 2014;42:1698-710 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Pazos P, Lima L, Casanueva F, Dieguez C, Garcia M. Interleukin 6 deficiency modulates the hypothalamic expression of energy balance regulating peptides during pregnancy in mice. PLoS ONE. 2013;8:e72339 pubmed publisher
  • western blot; human
Hosogane M, Funayama R, Nishida Y, Nagashima T, Nakayama K. Ras-induced changes in H3K27me3 occur after those in transcriptional activity. PLoS Genet. 2013;9:e1003698 pubmed publisher
  • western blot; mouse
Lu J, Chang Y, Wang C, Lin Y, Lin C, Wu Z. Trichostatin A modulates thiazolidinedione-mediated suppression of tumor necrosis factor ?-induced lipolysis in 3T3-L1 adipocytes. PLoS ONE. 2013;8:e71517 pubmed publisher
  • immunocytochemistry; human; 1:1000
  • western blot; human; 1:1000
Tan E, Caro S, Potnis A, Lanza C, Slawson C. O-linked N-acetylglucosamine cycling regulates mitotic spindle organization. J Biol Chem. 2013;288:27085-99 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Boutros R, Mondesert O, Lorenzo C, Astuti P, McArthur G, Chircop M, et al. CDC25B overexpression stabilises centrin 2 and promotes the formation of excess centriolar foci. PLoS ONE. 2013;8:e67822 pubmed publisher
  • western blot; mouse
van Boxtel R, Gomez Puerto C, Mokry M, Eijkelenboom A, van der Vos K, Nieuwenhuis E, et al. FOXP1 acts through a negative feedback loop to suppress FOXO-induced apoptosis. Cell Death Differ. 2013;20:1219-29 pubmed publisher
  • western blot; human
Li W, Notani D, Ma Q, Tanasa B, Nunez E, Chen A, et al. Functional roles of enhancer RNAs for oestrogen-dependent transcriptional activation. Nature. 2013;498:516-20 pubmed publisher
  • western blot; human; fig 2a
Tomida J, Itaya A, Shigechi T, Unno J, Uchida E, Ikura M, et al. A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair. Nucleic Acids Res. 2013;41:6930-41 pubmed publisher
  • western blot; rat
Kolupaeva V, Daempfling L, Basilico C. The B55? regulatory subunit of protein phosphatase 2A mediates fibroblast growth factor-induced p107 dephosphorylation and growth arrest in chondrocytes. Mol Cell Biol. 2013;33:2865-78 pubmed publisher
  • western blot; rat; 1:1000
Przewoźniak M, Czaplicka I, Czerwinska A, Markowska Zagrajek A, Moraczewski J, Stremińska W, et al. Adhesion proteins--an impact on skeletal myoblast differentiation. PLoS ONE. 2013;8:e61760 pubmed publisher
  • western blot; human; 1:10000
  • western blot; mouse; 1:10000
Scaramuzzino C, Monaghan J, Milioto C, Lanson N, Maltare A, Aggarwal T, et al. Protein arginine methyltransferase 1 and 8 interact with FUS to modify its sub-cellular distribution and toxicity in vitro and in vivo. PLoS ONE. 2013;8:e61576 pubmed publisher
  • western blot; rat; 1:10,000
Li X, Xiao Z, Han J, Chen L, Xiao H, Ma F, et al. Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair. Biomaterials. 2013;34:5107-16 pubmed publisher
  • western blot; mouse; 1:5000
Behrens A, Iacovino M, Lohr J, Ren Y, Zierold C, Harvey R, et al. Nkx2-5 mediates differential cardiac differentiation through interaction with Hoxa10. Stem Cells Dev. 2013;22:2211-20 pubmed publisher
  • immunocytochemistry; mouse
Trakala M, Fernández Miranda G, Perez de Castro I, Heeschen C, Malumbres M. Aurora B prevents delayed DNA replication and premature mitotic exit by repressing p21(Cip1). Cell Cycle. 2013;12:1030-41 pubmed publisher
  • immunocytochemistry; human; 1:400
Imbalzano K, Cohet N, Wu Q, Underwood J, Imbalzano A, Nickerson J. Nuclear shape changes are induced by knockdown of the SWI/SNF ATPase BRG1 and are independent of cytoskeletal connections. PLoS ONE. 2013;8:e55628 pubmed publisher
  • western blot; rat; 1:10000
Taylor D, Moser R, Regulier E, Breuillaud L, Dixon M, Beesen A, et al. MAP kinase phosphatase 1 (MKP-1/DUSP1) is neuroprotective in Huntington's disease via additive effects of JNK and p38 inhibition. J Neurosci. 2013;33:2313-25 pubmed publisher
  • immunocytochemistry; chicken; 1:2000
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Nasonkin I, Lazo K, Hambright D, Brooks M, Fariss R, Swaroop A. Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina. J Comp Neurol. 2011;519:1914-30 pubmed publisher
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product information
Product No. :
T5168
Product Name :
Monoclonal Anti-α-Tubulin antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
B-5-1-2
Applications/Description :
Suitable for indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts; western blot: 1:4,000 using human fibroblast cell extract; radioimmunoassay
Species Reactivity :
human, Chlamydomonas, African green monkey, chicken, kangaroo rat, bovine, mouse, rat, sea urchin
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA