product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse
catalog :
T0678
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
WH-3
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 2b
Hung C, Ono D, Kilduff T, Yamanaka A. Dual orexin and MCH neuron-ablated mice display severe sleep attacks and cataplexy. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1ii
Azimi Z, Barzan R, Spoida K, Surdin T, Wollenweber P, Mark M, et al. Separable gain control of ongoing and evoked activity in the visual cortex by serotonergic input. elife. 2020;9: pubmed publisher
  • immunohistochemistry; rat; 1:400; fig 1g1
Ghosh A, Torraville S, Mukherjee B, Walling S, Martin G, Harley C, et al. An experimental model of Braak's pretangle proposal for the origin of Alzheimer's disease: the role of locus coeruleus in early symptom development. Alzheimers Res Ther. 2019;11:59 pubmed publisher
  • immunohistochemistry; rat; 1:1000; loading ...; fig 5b
Kasper J, McCue D, Milton A, Szwed A, Sampson C, Huang M, et al. Gamma-Aminobutyric Acidergic Projections From the Dorsal Raphe to the Nucleus Accumbens Are Regulated by Neuromedin U. Biol Psychiatry. 2016;80:878-887 pubmed publisher
  • immunohistochemistry; mouse; 1:3000; loading ...; tbl 1
Sos K, Mayer M, Cserép C, Takács F, Szonyi A, Freund T, et al. Cellular architecture and transmitter phenotypes of neurons of the mouse median raphe region. Brain Struct Funct. 2017;222:287-299 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:1000; fig 4
Pomrenze M, Millan E, Hopf F, Keiflin R, Maiya R, Blasio A, et al. A Transgenic Rat for Investigating the Anatomy and Function of Corticotrophin Releasing Factor Circuits. Front Neurosci. 2015;9:487 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 4
Ehrich J, Messinger D, Knakal C, Kuhar J, Schattauer S, Bruchas M, et al. Kappa Opioid Receptor-Induced Aversion Requires p38 MAPK Activation in VTA Dopamine Neurons. J Neurosci. 2015;35:12917-31 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400
Dugué G, Lorincz M, Lottem E, Audero E, Matias S, Correia P, et al. Optogenetic recruitment of dorsal raphe serotonergic neurons acutely decreases mechanosensory responsivity in behaving mice. PLoS ONE. 2014;9:e105941 pubmed publisher
Wang H, Zhang S, Qi J, Wang H, Cachope R, Mejías Aponte C, et al. Dorsal Raphe Dual Serotonin-Glutamate Neurons Drive Reward by Establishing Excitatory Synapses on VTA Mesoaccumbens Dopamine Neurons. Cell Rep. 2019;26:1128-1142.e7 pubmed publisher
Bernal Meléndez E, Lacroix M, Bouillaud P, Callebert J, Olivier B, Persuy M, et al. Repeated gestational exposure to diesel engine exhaust affects the fetal olfactory system and alters olfactory-based behavior in rabbit offspring. Part Fibre Toxicol. 2019;16:5 pubmed publisher
Al Shareef S, Basit S, Li S, Pfister C, Pradervand S, Lecendreux M, et al. Kleine-Levin syndrome is associated with LMOD3 variants. J Sleep Res. 2019;28:e12718 pubmed publisher
Saito Y, Tsujino N, Abe M, Yamazaki M, Sakimura K, Sakurai T. Serotonergic Input to Orexin Neurons Plays a Role in Maintaining Wakefulness and REM Sleep Architecture. Front Neurosci. 2018;12:892 pubmed publisher
Zhang H, Sang N, Ge X, Huang Q, Li X, Sha J. Nesfatin-1 in the dorsal raphe nucleus influences visceral sensitivity via 5-HT neurons in male maternally separated rats. Sci Rep. 2018;8:9334 pubmed publisher
Sodersten E, Toskas K, Rraklli V, Tiklová K, Björklund A, Ringner M, et al. A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons. Nat Commun. 2018;9:1226 pubmed publisher
Zhang H, Zhao H, Zeng C, Van Dort C, Faingold C, Taylor N, et al. Optogenetic activation of 5-HT neurons in the dorsal raphe suppresses seizure-induced respiratory arrest and produces anticonvulsant effect in the DBA/1 mouse SUDEP model. Neurobiol Dis. 2018;110:47-58 pubmed publisher
Miyajima M, Zhang B, Sugiura Y, Sonomura K, Guerrini M, Tsutsui Y, et al. Metabolic shift induced by systemic activation of T cells in PD-1-deficient mice perturbs brain monoamines and emotional behavior. Nat Immunol. 2017;18:1342-1352 pubmed publisher
Prouty E, Chandler D, Waterhouse B. Neurochemical differences between target-specific populations of rat dorsal raphe projection neurons. Brain Res. 2017;1675:28-40 pubmed publisher
Kodani S, Soya S, Sakurai T. Excitation of GABAergic Neurons in the Bed Nucleus of the Stria Terminalis Triggers Immediate Transition from Non-Rapid Eye Movement Sleep to Wakefulness in Mice. J Neurosci. 2017;37:7164-7176 pubmed publisher
Puissant M, Mouradian G, Liu P, Hodges M. Identifying Candidate Genes that Underlie Cellular pH Sensitivity in Serotonin Neurons Using Transcriptomics: A Potential Role for Kir5.1 Channels. Front Cell Neurosci. 2017;11:34 pubmed publisher
Zhang M. Two-step production of monoamines in monoenzymatic cells in the spinal cord: a different control strategy of neurotransmitter supply?. Neural Regen Res. 2016;11:1904-1909 pubmed publisher
Winter A, Ahlbrand R, Naik D, Sah R. Differential behavioral sensitivity to carbon dioxide (CO2) inhalation in rats. Neuroscience. 2017;346:423-433 pubmed publisher
Cohen J, Ata A, Jackson N, Rahn E, Ramaker R, Cooper S, et al. Differential stress induced c-Fos expression and identification of region-specific miRNA-mRNA networks in the dorsal raphe and amygdala of high-responder/low-responder rats. Behav Brain Res. 2017;319:110-123 pubmed publisher
Totola L, Takakura A, Oliveira J, Garcia Cairasco N, Moreira T. Impaired central respiratory chemoreflex in an experimental genetic model of epilepsy. J Physiol. 2017;595:983-999 pubmed publisher
Marcinkiewcz C, Mazzone C, D Agostino G, Halladay L, Hardaway J, Diberto J, et al. Serotonin engages an anxiety and fear-promoting circuit in the extended amygdala. Nature. 2016;537:97-101 pubmed publisher
Zhang H, Zhao H, Yang X, Xue Q, Cotten J, Feng H. 5-Hydroxytryptophan, a precursor for serotonin synthesis, reduces seizure-induced respiratory arrest. Epilepsia. 2016;57:1228-35 pubmed publisher
Zhan Q, Buchanan G, Motelow J, Andrews J, Vitkovskiy P, Chen W, et al. Impaired Serotonergic Brainstem Function during and after Seizures. J Neurosci. 2016;36:2711-22 pubmed publisher
Kaplan K, Echert A, Massat B, Puissant M, Palygin O, Geurts A, et al. Chronic central serotonin depletion attenuates ventilation and body temperature in young but not adult Tph2 knockout rats. J Appl Physiol (1985). 2016;120:1070-81 pubmed publisher
Leong M, Speltz R, Wessendorf M. Effects of chronic constriction injury and spared nerve injury, two models of neuropathic pain, on the numbers of neurons and glia in the rostral ventromedial medulla. Neurosci Lett. 2016;617:82-7 pubmed publisher
Morton R, Yanagawa Y, Valenzuela C. Electrophysiological Assessment of Serotonin and GABA Neuron Function in the Dorsal Raphe during the Third Trimester Equivalent Developmental Period in Mice. Eneuro. 2015;2: pubmed publisher
Ruocco A, Rodrigo A, Carcone D, McMain S, Jacobs G, Kennedy J. Tryptophan hydroxylase 1 gene polymorphisms alter prefrontal cortex activation during response inhibition. Neuropsychology. 2016;30:18-27 pubmed publisher
Johansen S, Iceman K, Iceman C, Taylor B, Harris M. Isoflurane causes concentration-dependent inhibition of medullary raphé 5-HT neurons in situ. Auton Neurosci. 2015;193:51-6 pubmed publisher
Takahashi A, Lee R, Iwasato T, Itohara S, Arima H, Bettler B, et al. Glutamate input in the dorsal raphe nucleus as a determinant of escalated aggression in male mice. J Neurosci. 2015;35:6452-63 pubmed publisher
Wang D, Yau H, Broker C, Tsou J, Bonci A, Ikemoto S. Mesopontine median raphe regulates hippocampal ripple oscillation and memory consolidation. Nat Neurosci. 2015;18:728-35 pubmed publisher
Massey C, Iceman K, Johansen S, Wu Y, Harris M, Richerson G. Isoflurane abolishes spontaneous firing of serotonin neurons and masks their pH/COâ‚‚ chemosensitivity. J Neurophysiol. 2015;113:2879-88 pubmed publisher
Puissant M, Echert A, Yang C, Mouradian G, Novotny T, Liu P, et al. RNASeq-derived transcriptome comparisons reveal neuromodulatory deficiency in the CO₂ insensitive brown Norway rat. J Physiol. 2015;593:415-30 pubmed publisher
Cai Y, Wang W, Hou Y, Pan Z. Optogenetic activation of brainstem serotonergic neurons induces persistent pain sensitization. Mol Pain. 2014;10:70 pubmed publisher
Qi J, Zhang S, Wang H, Wang H, de Jesus Aceves Buendia J, Hoffman A, et al. A glutamatergic reward input from the dorsal raphe to ventral tegmental area dopamine neurons. Nat Commun. 2014;5:5390 pubmed publisher
Broms J, Antolin Fontes B, Tingström A, Ibañez Tallon I. Conserved expression of the GPR151 receptor in habenular axonal projections of vertebrates. J Comp Neurol. 2015;523:359-80 pubmed publisher
Lopez J, Lim R, Cruceanu C, Crapper L, Fasano C, Labonte B, et al. miR-1202 is a primate-specific and brain-enriched microRNA involved in major depression and antidepressant treatment. Nat Med. 2014;20:764-8 pubmed publisher
Goel N, Innala L, Viau V. Sex differences in serotonin (5-HT) 1A receptor regulation of HPA axis and dorsal raphe responses to acute restraint. Psychoneuroendocrinology. 2014;40:232-41 pubmed publisher
Corcoran A, Commons K, Wu Y, Smith J, Harris M, Richerson G. Dual effects of 5-HT(1a) receptor activation on breathing in neonatal mice. J Neurosci. 2014;34:51-9 pubmed publisher
Meredith M, May J. Regulation of embryonic neurotransmitter and tyrosine hydroxylase protein levels by ascorbic acid. Brain Res. 2013;1539:7-14 pubmed publisher
Wang C, Lin H, Chan Y, Gean P, Yang Y, Chen P. 5-HT1A-receptor agonist modified amygdala activity and amygdala-associated social behavior in a valproate-induced rat autism model. Int J Neuropsychopharmacol. 2013;16:2027-39 pubmed publisher
Andre K, Kampman O, Viikki M, Illi A, Setälä Soikkeli E, Poutanen O, et al. TPH1 A218C polymorphism and temperament in major depression. BMC Psychiatry. 2013;13:118 pubmed publisher
Katter K, Geurts A, Hoffmann O, Mátés L, Landa V, Hiripi L, et al. Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits. FASEB J. 2013;27:930-41 pubmed publisher
Jun S, Kohen R, Cain K, Jarrett M, Heitkemper M. TPH gene polymorphisms are associated with disease perception and quality of life in women with irritable bowel syndrome. Biol Res Nurs. 2014;16:95-104 pubmed publisher
Lee S, Kirigiti M, Lindsley S, Loche A, Madden C, Morrison S, et al. Efferent projections of neuropeptide Y-expressing neurons of the dorsomedial hypothalamus in chronic hyperphagic models. J Comp Neurol. 2013;521:1891-914 pubmed publisher
Shikanai H, Yoshida T, Konno K, Yamasaki M, Izumi T, Ohmura Y, et al. Distinct neurochemical and functional properties of GAD67-containing 5-HT neurons in the rat dorsal raphe nucleus. J Neurosci. 2012;32:14415-26 pubmed publisher
Sikandar S, Bannister K, Dickenson A. Brainstem facilitations and descending serotonergic controls contribute to visceral nociception but not pregabalin analgesia in rats. Neurosci Lett. 2012;519:31-6 pubmed publisher
Lacerda C, Kisiday J, Johnson B, Orton E. Local serotonin mediates cyclic strain-induced phenotype transformation, matrix degradation, and glycosaminoglycan synthesis in cultured sheep mitral valves. Am J Physiol Heart Circ Physiol. 2012;302:H1983-90 pubmed publisher
Leong M, Gu M, Speltz Paiz R, Stahura E, Mottey N, Steer C, et al. Neuronal loss in the rostral ventromedial medulla in a rat model of neuropathic pain. J Neurosci. 2011;31:17028-39 pubmed publisher
Crawford L, Craige C, Beck S. Glutamatergic input is selectively increased in dorsal raphe subfield 5-HT neurons: role of morphology, topography and selective innervation. Eur J Neurosci. 2011;34:1794-806 pubmed publisher
Zhao S, Ting J, Atallah H, Qiu L, Tan J, Gloss B, et al. Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function. Nat Methods. 2011;8:745-52 pubmed
Calizo L, Akanwa A, Ma X, Pan Y, Lemos J, Craige C, et al. Raphe serotonin neurons are not homogenous: electrophysiological, morphological and neurochemical evidence. Neuropharmacology. 2011;61:524-43 pubmed publisher
Hodges M, Best S, Richerson G. Altered ventilatory and thermoregulatory control in male and female adult Pet-1 null mice. Respir Physiol Neurobiol. 2011;177:133-40 pubmed publisher
Lukkes J, Staub D, Dietrich A, Truitt W, Neufeld Cohen A, Chen A, et al. Topographical distribution of corticotropin-releasing factor type 2 receptor-like immunoreactivity in the rat dorsal raphe nucleus: co-localization with tryptophan hydroxylase. Neuroscience. 2011;183:47-63 pubmed publisher
Carkaci Salli N, Salli U, Kuntz Melcavage K, Pennock M, Ozgen H, Tekin I, et al. TPH2 in the ventral tegmental area of the male rat brain. Brain Res Bull. 2011;84:376-80 pubmed publisher
Liu Q, Wong Riley M. Postnatal changes in tryptophan hydroxylase and serotonin transporter immunoreactivity in multiple brainstem nuclei of the rat: implications for a sensitive period. J Comp Neurol. 2010;518:1082-97 pubmed publisher
Heinisch S, Kirby L. SDF-1alpha/CXCL12 enhances GABA and glutamate synaptic activity at serotonin neurons in the rat dorsal raphe nucleus. Neuropharmacology. 2010;58:501-14 pubmed publisher
Saetre P, Lundmark P, Wang A, Hansen T, Rasmussen H, Djurovic S, et al. The tryptophan hydroxylase 1 (TPH1) gene, schizophrenia susceptibility, and suicidal behavior: a multi-centre case-control study and meta-analysis. Am J Med Genet B Neuropsychiatr Genet. 2010;153B:387-396 pubmed publisher
Hale M, Hay Schmidt A, Mikkelsen J, Poulsen B, Bouwknecht J, Evans A, et al. Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex. Neuroscience. 2008;157:733-48 pubmed publisher
Kish S, Fitzmaurice P, Boileau I, Schmunk G, Ang L, Furukawa Y, et al. Brain serotonin transporter in human methamphetamine users. Psychopharmacology (Berl). 2009;202:649-61 pubmed publisher
Cano G, Mochizuki T, Saper C. Neural circuitry of stress-induced insomnia in rats. J Neurosci. 2008;28:10167-84 pubmed publisher
Mulkey D, Rosin D, West G, Takakura A, Moreira T, Bayliss D, et al. Serotonergic neurons activate chemosensitive retrotrapezoid nucleus neurons by a pH-independent mechanism. J Neurosci. 2007;27:14128-38 pubmed
Kish S, Tong J, Hornykiewicz O, Rajput A, Chang L, Guttman M, et al. Preferential loss of serotonin markers in caudate versus putamen in Parkinson's disease. Brain. 2008;131:120-31 pubmed
Toney G, Daws L. Juxtacellular labeling and chemical phenotyping of extracellularly recorded neurons in vivo. Methods Mol Biol. 2006;337:127-37 pubmed
Commons K, Beck S, Bey V. Two populations of glutamatergic axons in the rat dorsal raphe nucleus defined by the vesicular glutamate transporters 1 and 2. Eur J Neurosci. 2005;21:1577-86 pubmed
Cote F, Thévenot E, Fligny C, Fromes Y, Darmon M, Ripoche M, et al. Disruption of the nonneuronal tph1 gene demonstrates the importance of peripheral serotonin in cardiac function. Proc Natl Acad Sci U S A. 2003;100:13525-30 pubmed
Burman K, Ige A, White J, Marshall F, Pangalos M, Emson P, et al. GABAB receptor subunits, R1 and R2, in brainstem catecholamine and serotonin neurons. Brain Res. 2003;970:35-46 pubmed
Nguyen T, Chin W, O Brien J, Verdugo P, Berger A. Intracellular pathways regulating ciliary beating of rat brain ependymal cells. J Physiol. 2001;531:131-40 pubmed
product information
Product No. :
T0678
Product Name :
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
WH-3
Applications/Description :
Suitable for immunohistochemistry; western blot: 1:1,000 using rabbit pineal gland extract; microarray; indirect ELISA
Species Reactivity :
monkey, rat, human, rabbit
Isotype :
IgG3
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA