product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-AGO2 antibody produced in rat
catalog :
SAB4200085
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
11A9
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, RNA immunoprecipitation, western blot knockout validation
more info or order :
citations: 42
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig 4c
  • chromatin immunoprecipitation; human; loading ...; fig 3a
van Eijl R, van den Brand T, Nguyen L, Mulder K. Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity. Sci Rep. 2017;7:7278 pubmed publisher
  • western blot knockout validation; human; loading ...; fig s3a
Golden R, Chen B, Li T, Braun J, Manjunath H, Chen X, et al. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017;542:197-202 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2d
Li Y, Wang L, Rivera Serrano E, Chen X, Lemon S. TNRC6 proteins modulate hepatitis C virus replication by spatially regulating the binding of miR-122/Ago2 complexes to viral RNA. Nucleic Acids Res. 2019;47:6411-6424 pubmed publisher
  • western blot; human; loading ...; fig s3g, 3b, 3c, s3a
Jeppesen D, Fenix A, Franklin J, Higginbotham J, Zhang Q, Zimmerman L, et al. Reassessment of Exosome Composition. Cell. 2019;177:428-445.e18 pubmed publisher
  • immunoprecipitation; rat; fig 4h
Periyasamy P, Liao K, Kook Y, Niu F, Callen S, Guo M, et al. Cocaine-Mediated Downregulation of miR-124 Activates Microglia by Targeting KLF4 and TLR4 Signaling. Mol Neurobiol. 2018;55:3196-3210 pubmed publisher
  • immunoprecipitation; human; fig 4
Rasmussen M, Lyskjær I, Jersie Christensen R, Tarpgaard L, Primdal Bengtson B, Nielsen M, et al. miR-625-3p regulates oxaliplatin resistance by targeting MAP2K6-p38 signalling in human colorectal adenocarcinoma cells. Nat Commun. 2016;7:12436 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 4b
Miyazaki Y, Du X, Muramatsu S, Gomez C. An miRNA-mediated therapy for SCA6 blocks IRES-driven translation of the CACNA1A second cistron. Sci Transl Med. 2016;8:347ra94 pubmed publisher
  • western blot; human; 1:1000; fig s8
Salzman D, Nakamura K, Nallur S, Dookwah M, Metheetrairut C, Slack F, et al. miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage. Nat Commun. 2016;7:10954 pubmed publisher
  • RNA immunoprecipitation; human
Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  • western blot; human; fig 2c
van Buggenum J, Gerlach J, Eising S, Schoonen L, van Eijl R, Tanis S, et al. A covalent and cleavable antibody-DNA conjugation strategy for sensitive protein detection via immuno-PCR. Sci Rep. 2016;6:22675 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Boudoukha S, Rivera Vargas T, Dang I, Kropp J, Cuvellier S, Gautreau A, et al. MiRNA let-7g regulates skeletal myoblast motility via Pinch-2. FEBS Lett. 2014;588:1623-9 pubmed publisher
Qiu N, Xu X, He Y. LncRNA TUG1 alleviates sepsis-induced acute lung injury by targeting miR-34b-5p/GAB1. BMC Pulm Med. 2020;20:49 pubmed publisher
Yang Q, Li R, Lyu Q, Hou L, Liu Z, Sun Q, et al. Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes. Nat Commun. 2019;10:3389 pubmed publisher
Most D, Salem N, Tiwari G, Blednov Y, Mayfield R, Harris R. Silencing synaptic MicroRNA-411 reduces voluntary alcohol consumption in mice. Addict Biol. 2019;24:604-616 pubmed publisher
Nowakowski T, Rani N, Golkaram M, Zhou H, Alvarado B, Huch K, et al. Regulation of cell-type-specific transcriptomes by microRNA networks during human brain development. Nat Neurosci. 2018;21:1784-1792 pubmed publisher
Kutsche L, Gysi D, Fallmann J, Lenk K, Petri R, Swiersy A, et al. Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis. Cell Syst. 2018;7:438-452.e8 pubmed publisher
Periyasamy P, Thangaraj A, Guo M, Hu G, Callen S, Buch S. Epigenetic Promoter DNA Methylation of miR-124 Promotes HIV-1 Tat-Mediated Microglial Activation via MECP2-STAT3 Axis. J Neurosci. 2018;38:5367-5383 pubmed publisher
Ottaviani S, Stebbing J, Frampton A, Zagorac S, Krell J, de Giorgio A, et al. TGF-β induces miR-100 and miR-125b but blocks let-7a through LIN28B controlling PDAC progression. Nat Commun. 2018;9:1845 pubmed publisher
Zhang T, Wu Y, Mullane P, Ji Y, Liu H, He L, et al. FUS Regulates Activity of MicroRNA-Mediated Gene Silencing. Mol Cell. 2018;69:787-801.e8 pubmed publisher
Lee Y, Bernstock J, Klimanis D, Hallenbeck J. Akt Protein Kinase, miR-200/miR-182 Expression and Epithelial-Mesenchymal Transition Proteins in Hibernating Ground Squirrels. Front Mol Neurosci. 2018;11:22 pubmed publisher
Awan H, Shah A, Rashid F, Wei S, Chen L, Shan G. Comparing two approaches of miR-34a target identification, biotinylated-miRNA pulldown vs miRNA overexpression. RNA Biol. 2017;:1-7 pubmed publisher
Sahoo M, Gaikwad S, Khuperkar D, Ashok M, Helen M, Yadav S, et al. Nup358 binds to AGO proteins through its SUMO-interacting motifs and promotes the association of target mRNA with miRISC. EMBO Rep. 2017;18:241-263 pubmed publisher
Deforzh E, Vargas T, Kropp J, Vandamme M, Pinna G, Polesskaya A. IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. Int J Oncol. 2016;49:2578-2588 pubmed publisher
Ouyang Y, Bayer A, Chu T, Tyurin V, Kagan V, Morelli A, et al. Isolation of human trophoblastic extracellular vesicles and characterization of their cargo and antiviral activity. Placenta. 2016;47:86-95 pubmed publisher
Dasgupta N, Peng Y, Tan Z, Ciraolo G, Wang D, Li R. miRNAs in mtDNA-less cell mitochondria. Cell Death Discov. 2015;1:15004 pubmed publisher
Huang C, Wang X, Liu X, Cao S, Shan G. RNAi pathway participates in chromosome segregation in mammalian cells. Cell Discov. 2015;1:15029 pubmed publisher
Angart P, Carlson R, Adu Berchie K, Walton S. Terminal Duplex Stability and Nucleotide Identity Differentially Control siRNA Loading and Activity in RNA Interference. Nucleic Acid Ther. 2016;26:309-317 pubmed
Kratassiouk G, Pritchard L, Cuvellier S, Vislovukh A, Meng Q, Groisman R, et al. The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M. Cell Cycle. 2016;15:667-77 pubmed publisher
Krell J, Stebbing J, Carissimi C, Dabrowska A, de Giorgio A, Frampton A, et al. TP53 regulates miRNA association with AGO2 to remodel the miRNA-mRNA interaction network. Genome Res. 2016;26:331-41 pubmed publisher
Durruthy Durruthy J, Sebastiano V, Wossidlo M, Cepeda D, Cui J, Grow E, et al. The primate-specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming. Nat Genet. 2016;48:44-52 pubmed publisher
Nixon B, Stanger S, Mihalas B, Reilly J, Anderson A, Dun M, et al. Next Generation Sequencing Analysis Reveals Segmental Patterns of microRNA Expression in Mouse Epididymal Epithelial Cells. PLoS ONE. 2015;10:e0135605 pubmed publisher
Abuhatzira L, Xu H, Tahhan G, Boulougoura A, Schäffer A, Notkins A. Multiple microRNAs within the 14q32 cluster target the mRNAs of major type 1 diabetes autoantigens IA-2, IA-2β, and GAD65. FASEB J. 2015;29:4374-83 pubmed publisher
Lewkowicz P, Cwiklińska H, Mycko M, Cichalewska M, Domowicz M, Lewkowicz N, et al. Dysregulated RNA-Induced Silencing Complex (RISC) Assembly within CNS Corresponds with Abnormal miRNA Expression during Autoimmune Demyelination. J Neurosci. 2015;35:7521-37 pubmed publisher
Mukherjee A, Di Bisceglie A, Ray R. Hepatitis C virus-mediated enhancement of microRNA miR-373 impairs the JAK/STAT signaling pathway. J Virol. 2015;89:3356-65 pubmed publisher
Seto E, Inoue T, Nakatani Y, Yamada M, Isomura H. Processing bodies accumulate in human cytomegalovirus-infected cells and do not affect viral replication at high multiplicity of infection. Virology. 2014;458-459:151-61 pubmed publisher
Holla S, Kurowska Stolarska M, Bayry J, Balaji K. Selective inhibition of IFNG-induced autophagy by Mir155- and Mir31-responsive WNT5A and SHH signaling. Autophagy. 2014;10:311-30 pubmed publisher
Roberts A, Doidge R, Tarr A, Jopling C. The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122. Nucleic Acids Res. 2014;42:1257-69 pubmed publisher
Nechaev S, Gao C, Moreira D, Swiderski P, Jóźwiak A, Kowolik C, et al. Intracellular processing of immunostimulatory CpG-siRNA: Toll-like receptor 9 facilitates siRNA dicing and endosomal escape. J Control Release. 2013;170:307-15 pubmed publisher
Maurin T, Cazalla D, Yang S, Bortolamiol Becet D, Lai E. RNase III-independent microRNA biogenesis in mammalian cells. RNA. 2012;18:2166-73 pubmed publisher
Turchinovich A, Burwinkel B. Distinct AGO1 and AGO2 associated miRNA profiles in human cells and blood plasma. RNA Biol. 2012;9:1066-75 pubmed publisher
Rüdel S, Flatley A, Weinmann L, Kremmer E, Meister G. A multifunctional human Argonaute2-specific monoclonal antibody. RNA. 2008;14:1244-53 pubmed publisher
Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell. 2004;15:185-97 pubmed
product information
Product No. :
SAB4200085
Product Name :
Monoclonal Anti-AGO2 antibody produced in rat
Host :
rat
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
11A9
Applications/Description :
Suitable for immunocytochemistry; western blot: 0.5-1.0 μg/mL using HeLa cell extracts; immunoprecipitation (IP)
Species Reactivity :
mouse, human, rat, monkey, bovine
Isotype :
IgG2a
Concentration :
~1.5 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA