product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Anti-Vinculin antibody, Mouse monoclonal
catalog :
SAB4200080
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
V284
reactivity :
human, mouse, rat, dogs, chicken
application :
western blot, immunoprecipitation
more info or order :
citations: 33
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 1
Radzisheuskaya A, Shlyueva D, Muller I, Helin K. Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic Acids Res. 2016;44:e141 pubmed
Ferreira C, Sumner R, Rodriguez Plata M, Rasaiyaah J, Milne R, Thrasher A, et al. Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity. Mol Ther Methods Clin Dev. 2020;17:209-219 pubmed publisher
Radzisheuskaya A, Shliaha P, Grinev V, Lorenzini E, Kovalchuk S, Shlyueva D, et al. PRMT5 methylome profiling uncovers a direct link to splicing regulation in acute myeloid leukemia. Nat Struct Mol Biol. 2019;26:999-1012 pubmed publisher
Santos L, Durães C, Ziros P, Pestana A, Esteves C, Neves C, et al. Interaction of Genetic Variations in NFE2L2 and SELENOS Modulates the Risk of Hashimoto's Thyroiditis. Thyroid. 2019;29:1302-1315 pubmed publisher
Nowak M, Suenkel B, Porras P, Migotti R, Schmidt F, Kny M, et al. DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy. J Cell Sci. 2019;132: pubmed publisher
Avalle L, Camporeale A, Morciano G, Caroccia N, Ghetti E, Orecchia V, et al. STAT3 localizes to the ER, acting as a gatekeeper for ER-mitochondrion Ca2+ fluxes and apoptotic responses. Cell Death Differ. 2019;26:932-942 pubmed publisher
Joshi P, Riley D, Khalil J, Xiong H, Ji W, Rivero F. The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation. Sci Rep. 2018;8:10804 pubmed publisher
Ziros P, Habeos I, Chartoumpekis D, Ntalampyra E, Somm E, Renaud C, et al. NFE2-Related Transcription Factor 2 Coordinates Antioxidant Defense with Thyroglobulin Production and Iodination in the Thyroid Gland. Thyroid. 2018;28:780-798 pubmed publisher
Meiser J, Schuster A, Pietzke M, Vande Voorde J, Athineos D, Oizel K, et al. Increased formate overflow is a hallmark of oxidative cancer. Nat Commun. 2018;9:1368 pubmed publisher
Huang H, Dobrovolsky D, Paulk J, Yang G, Weisberg E, Doctor Z, et al. A Chemoproteomic Approach to Query the Degradable Kinome Using a Multi-kinase Degrader. Cell Chem Biol. 2018;25:88-99.e6 pubmed publisher
Fusella F, Seclì L, Busso E, Krepelova A, Moiso E, Rocca S, et al. The IKK/NF-κB signaling pathway requires Morgana to drive breast cancer metastasis. Nat Commun. 2017;8:1636 pubmed publisher
Huang H, Seo H, Zhang T, Wang Y, Jiang B, Li Q, et al. MELK is not necessary for the proliferation of basal-like breast cancer cells. elife. 2017;6: pubmed publisher
Malerba A, Klein P, Bachtarzi H, Jarmin S, Córdova G, Ferry A, et al. PABPN1 gene therapy for oculopharyngeal muscular dystrophy. Nat Commun. 2017;8:14848 pubmed publisher
Bolino A, Piguet F, Alberizzi V, Pellegatta M, Rivellini C, Guerrero Valero M, et al. Niacin-mediated Tace activation ameliorates CMT neuropathies with focal hypermyelination. EMBO Mol Med. 2016;8:1438-1454 pubmed publisher
Krepler C, Xiao M, Samanta M, Vultur A, Chen H, Brafford P, et al. Targeting Notch enhances the efficacy of ERK inhibitors in BRAF-V600E melanoma. Oncotarget. 2016;7:71211-71222 pubmed publisher
Izard T, Brown D. Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites. J Biol Chem. 2016;291:2548-55 pubmed publisher
Benatti P, Chiaramonte M, Lorenzo M, Hartley J, Hochhauser D, Gnesutta N, et al. NF-Y activates genes of metabolic pathways altered in cancer cells. Oncotarget. 2016;7:1633-50 pubmed publisher
Pesciotta E, Lam H, Kossenkov A, Ge J, Showe L, Mason P, et al. In-Depth, Label-Free Analysis of the Erythrocyte Cytoplasmic Proteome in Diamond Blackfan Anemia Identifies a Unique Inflammatory Signature. PLoS ONE. 2015;10:e0140036 pubmed publisher
Case L, Baird M, Shtengel G, Campbell S, Hess H, Davidson M, et al. Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions. Nat Cell Biol. 2015;17:880-92 pubmed publisher
Braun G, Nagayama Y, Maruta Y, Heymann F, van Roeyen C, Klinkhammer B, et al. IL-6 Trans-Signaling Drives Murine Crescentic GN. J Am Soc Nephrol. 2016;27:132-42 pubmed publisher
Yamana S, Tokiyama A, Mizutani K, Hirata K, Takai Y, Rikitake Y. The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation. PLoS ONE. 2015;10:e0124259 pubmed publisher
van der Ent W, Burrello C, Teunisse A, Ksander B, van der Velden P, Jager M, et al. Modeling of human uveal melanoma in zebrafish xenograft embryos. Invest Ophthalmol Vis Sci. 2014;55:6612-22 pubmed publisher
Rubashkin M, Cassereau L, Bainer R, DuFort C, Yui Y, Ou G, et al. Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate. Cancer Res. 2014;74:4597-611 pubmed publisher
Choi P, Li Y, Felsher D. Addiction to multiple oncogenes can be exploited to prevent the emergence of therapeutic resistance. Proc Natl Acad Sci U S A. 2014;111:E3316-24 pubmed publisher
Galletti M, Cantoni S, Zambelli F, Valente S, Palazzini M, Manes A, et al. Dissecting histone deacetylase role in pulmonary arterial smooth muscle cell proliferation and migration. Biochem Pharmacol. 2014;91:181-90 pubmed publisher
Brack S, Attinger Toller I, Schade B, Mourlane F, Klupsch K, Woods R, et al. A bispecific HER2-targeting FynomAb with superior antitumor activity and novel mode of action. Mol Cancer Ther. 2014;13:2030-9 pubmed publisher
Grassilli E, Ianzano L, Bonomo S, Missaglia C, Cerrito M, Giovannoni R, et al. GSK3A is redundant with GSK3B in modulating drug resistance and chemotherapy-induced necroptosis. PLoS ONE. 2014;9:e100947 pubmed publisher
van der Ent W, Jochemsen A, Teunisse A, Krens S, Szuhai K, Spaink H, et al. Ewing sarcoma inhibition by disruption of EWSR1-FLI1 transcriptional activity and reactivation of p53. J Pathol. 2014;233:415-24 pubmed publisher
Jung K, Wu F, Wang P, Ye X, Abdulkarim B, Lai R. YB-1 regulates Sox2 to coordinately sustain stemness and tumorigenic properties in a phenotypically distinct subset of breast cancer cells. BMC Cancer. 2014;14:328 pubmed publisher
Bendris N, Cheung C, Leong H, Lewis J, Chambers A, Blanchard J, et al. Cyclin A2, a novel regulator of EMT. Cell Mol Life Sci. 2014;71:4881-94 pubmed publisher
Knight D, Gillies E, Mequanint K. Biomimetic L-aspartic acid-derived functional poly(ester amide)s for vascular tissue engineering. Acta Biomater. 2014;10:3484-96 pubmed publisher
Jin L, Hastings N, Blackman B, Somlyo A. Mechanical properties of the extracellular matrix alter expression of smooth muscle protein LPP and its partner palladin; relationship to early atherosclerosis and vascular injury. J Muscle Res Cell Motil. 2009;30:41-55 pubmed publisher
Porter R, Holme T, Newman E, Hopwood D, Wilkinson J, Cuschieri A. Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer. J Pathol. 1993;170:435-40 pubmed
product information
Product No. :
SAB4200080
Product Name :
Anti-Vinculin antibody, Mouse monoclonal
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
V284
Applications/Description :
Suitable for immunocytochemistry: 4-8 μg/mL using HeLa cells fixed with methanol:acetone; western blot: 0.005-0.01 μg/mL using HeLa total cell extracts; immunoprecipitation (IP)
Species Reactivity :
mouse, human, chicken, rat, canine, monkey, rabbit
Isotype :
IgG1
Concentration :
~1 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA