product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse
catalog :
S4045
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SC-35
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 108
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig s4c
Wheeler J, McMillan P, Strovas T, Liachko N, Amlie Wolf A, Kow R, et al. Activity of the poly(A) binding protein MSUT2 determines susceptibility to pathological tau in the mammalian brain. Sci Transl Med. 2019;11: pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 2e
Tanaka H, Kondo K, Chen X, Homma H, Tagawa K, Kerever A, et al. The intellectual disability gene PQBP1 rescues Alzheimer's disease pathology. Mol Psychiatry. 2018;23:2090-2110 pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; fig 2a
Rai A, Chen J, Selbach M, Pelkmans L. Kinase-controlled phase transition of membraneless organelles in mitosis. Nature. 2018;559:211-216 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 1e
  • immunocytochemistry; human; loading ...; fig 2b
Kuga T, Kume H, Adachi J, Kawasaki N, Shimizu M, Hoshino I, et al. Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON. Sci Rep. 2016;6:34472 pubmed publisher
  • immunocytochemistry; human; 1:5000; fig 1
Even Y, Escande M, Fayet C, Geneviere A. CDK13, a Kinase Involved in Pre-mRNA Splicing, Is a Component of the Perinucleolar Compartment. PLoS ONE. 2016;11:e0149184 pubmed publisher
  • immunocytochemistry; human; fig s2
Ohsaki Y, Kawai T, Yoshikawa Y, Cheng J, Jokitalo E, Fujimoto T. PML isoform II plays a critical role in nuclear lipid droplet formation. J Cell Biol. 2016;212:29-38 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 6a
Hübner B, Lomiento M, Mammoli F, Illner D, Markaki Y, Ferrari S, et al. Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments. Epigenetics Chromatin. 2015;8:47 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 3
Walker E, Jensen L, Croft S, Ghildyal R. Variation in the nuclear effects of infection by different human rhinovirus serotypes. Front Microbiol. 2015;6:875 pubmed publisher
  • immunocytochemistry; human; 1:200; fig s3
Stockley J, Markert E, Zhou Y, Robson C, Elliott D, Lindberg J, et al. The RNA-binding protein Sam68 regulates expression and transcription function of the androgen receptor splice variant AR-V7. Sci Rep. 2015;5:13426 pubmed publisher
  • immunocytochemistry; human; fig 7a
Lefèvre L, Omeiri H, Drougat L, Hantel C, Giraud M, Val P, et al. Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of β-catenin in adrenocortical carcinoma. Oncogenesis. 2015;4:e161 pubmed publisher
  • immunocytochemistry; human; fig 1b
Chang S, Chang W, Lu C, Tarn W. Alanine repeats influence protein localization in splicing speckles and paraspeckles. Nucleic Acids Res. 2014;42:13788-98 pubmed publisher
  • immunocytochemistry; human
Hett A, West S. Inhibition of U4 snRNA in human cells causes the stable retention of polyadenylated pre-mRNA in the nucleus. PLoS ONE. 2014;9:e96174 pubmed publisher
  • immunocytochemistry; human; 1:2000
Hu Y, Ericsson I, Doseth B, Liabakk N, Krokan H, Kavli B. Activation-induced cytidine deaminase (AID) is localized to subnuclear domains enriched in splicing factors. Exp Cell Res. 2014;322:178-92 pubmed publisher
  • immunocytochemistry; human; fig s1
Tsuiji H, Iguchi Y, Furuya A, Kataoka A, Hatsuta H, Atsuta N, et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol Med. 2013;5:221-34 pubmed publisher
Damodaran A, Courtheoux T, Watrin E, Prigent C. Alteration of SC35 localization by transfection reagents. Biochim Biophys Acta Mol Cell Res. 2020;1867:118650 pubmed publisher
Capponi S, Stöffler N, Irimia M, van Schaik F, Ondik M, Biniossek M, et al. Neuronal-specific microexon splicing of TAF1 mRNA is directly regulated by SRRM4/nSR100. RNA Biol. 2020;17:62-74 pubmed publisher
Moreno Castro C, Prieto Sánchez S, Sánchez Hernández N, Hernández Munain C, Suñé C. Role for the splicing factor TCERG1 in Cajal body integrity and snRNP assembly. J Cell Sci. 2019;132: pubmed publisher
Oqani R, Lin T, Lee J, Kang J, Shin H, Il Jin D. Iws1 and Spt6 Regulate Trimethylation of Histone H3 on Lysine 36 through Akt Signaling and are Essential for Mouse Embryonic Genome Activation. Sci Rep. 2019;9:3831 pubmed publisher
Gonatopoulos Pournatzis T, Wu M, Braunschweig U, Roth J, Han H, Best A, et al. Genome-wide CRISPR-Cas9 Interrogation of Splicing Networks Reveals a Mechanism for Recognition of Autism-Misregulated Neuronal Microexons. Mol Cell. 2018;72:510-524.e12 pubmed publisher
Ilicheva N, Podgornaya O, Bogolyubov D, Pochukalina G. The karyosphere capsule in Rana temporaria oocytes contains structural and DNA-binding proteins. Nucleus. 2018;9:516-529 pubmed publisher
Chen Y, Zhang Y, Wang Y, Zhang L, Brinkman E, Adam S, et al. Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler. J Cell Biol. 2018;217:4025-4048 pubmed publisher
Flather D, Nguyen J, Semler B, Gershon P. Exploitation of nuclear functions by human rhinovirus, a cytoplasmic RNA virus. PLoS Pathog. 2018;14:e1007277 pubmed publisher
Lebrun N, Giurgea I, Goldenberg A, Dieux A, Afenjar A, Ghoumid J, et al. Molecular and cellular issues of KMT2A variants involved in Wiedemann-Steiner syndrome. Eur J Hum Genet. 2018;26:107-116 pubmed publisher
Stachecka J, Walczak A, Kociucka B, Ruszczycki B, Wilczynski G, Szczerbal I. Nuclear organization during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes. Histochem Cell Biol. 2018;149:113-126 pubmed publisher
Hou W, Cruz Cosme R, Armstrong N, Obwolo L, Wen F, Hu W, et al. Molecular cloning and characterization of the genes encoding the proteins of Zika virus. Gene. 2017;628:117-128 pubmed publisher
Aktas T, Avşar Ilık İ, Maticzka D, Bhardwaj V, Pessoa Rodrigues C, Mittler G, et al. DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome. Nature. 2017;544:115-119 pubmed publisher
Li R, Harvey A, Hodgetts S, Fox A. Functional dissection of NEAT1 using genome editing reveals substantial localization of the NEAT1_1 isoform outside paraspeckles. RNA. 2017;23:872-881 pubmed publisher
Paolella B, Gibson W, Urbanski L, Alberta J, Zack T, Bandopadhayay P, et al. Copy-number and gene dependency analysis reveals partial copy loss of wild-type SF3B1 as a novel cancer vulnerability. elife. 2017;6: pubmed publisher
Pochukalina G, Ilicheva N, Podgornaya O, Voronin A. Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II. Mol Cytogenet. 2016;9:50 pubmed publisher
Oqani R, Lin T, Lee J, Choi K, Shin H, Jin D. P-TEFb Kinase Activity Is Essential for Global Transcription, Resumption of Meiosis and Embryonic Genome Activation in Pig. PLoS ONE. 2016;11:e0152254 pubmed publisher
Sánchez Hernández N, Boireau S, Schmidt U, Muñoz Cobo J, Hernández Munain C, Bertrand E, et al. The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing. RNA. 2016;22:571-82 pubmed publisher
Rice B, KADDIS R, Stake M, Lochmann T, Parent L. Interplay between the alpharetroviral Gag protein and SR proteins SF2 and SC35 in the nucleus. Front Microbiol. 2015;6:925 pubmed publisher
Tresini M, Warmerdam D, Kolovos P, Snijder L, Vrouwe M, Demmers J, et al. The core spliceosome as target and effector of non-canonical ATM signalling. Nature. 2015;523:53-8 pubmed publisher
Abe K, Yamamoto R, Franke V, Cao M, Suzuki Y, Suzuki M, et al. The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing. EMBO J. 2015;34:1523-37 pubmed publisher
Díaz Hernández J, Sebastián Serrano A, Gomez Villafuertes R, Diaz Hernandez M, Miras Portugal M. Age-related nuclear translocation of P2X6 subunit modifies splicing activity interacting with splicing factor 3A1. PLoS ONE. 2015;10:e0123121 pubmed publisher
Silva A, Brown J, Buckle V, Wade Martins R, Lufino M. Expanded GAA repeats impair FXN gene expression and reposition the FXN locus to the nuclear lamina in single cells. Hum Mol Genet. 2015;24:3457-71 pubmed publisher
Arai T, Kano F, Murata M. Translocation of forkhead box O1 to the nuclear periphery induces histone modifications that regulate transcriptional repression of PCK1 in HepG2 cells. Genes Cells. 2015;20:340-57 pubmed publisher
Becerra S, Montes M, Hernández Munain C, Suñé C. Prp40 pre-mRNA processing factor 40 homolog B (PRPF40B) associates with SF1 and U2AF65 and modulates alternative pre-mRNA splicing in vivo. RNA. 2015;21:438-57 pubmed publisher
Snyers L, Zupkovitz G, Almeder M, Fliesser M, Stoisser A, Weipoltshammer K, et al. Distinct chromatin signature of histone H3 variant H3.3 in human cells. Nucleus. 2014;5:449-61 pubmed publisher
Zhang H, Mahadevan K, Palazzo A. Sumoylation is Required for the Cytoplasmic Accumulation of a Subset of mRNAs. Genes (Basel). 2014;5:982-1000 pubmed publisher
Fulka H, Langerova A. The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition. Development. 2014;141:1694-704 pubmed publisher
Ping X, Sun B, Wang L, Xiao W, Yang X, Wang W, et al. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. Cell Res. 2014;24:177-89 pubmed publisher
Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, et al. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nat Chem Biol. 2014;10:93-5 pubmed publisher
Horiuchi K, Kawamura T, Iwanari H, Ohashi R, Naito M, Kodama T, et al. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem. 2013;288:33292-302 pubmed publisher
Sasaki Osugi K, Imoto C, Takahara T, Shibata H, Maki M. Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA. J Biol Chem. 2013;288:33361-75 pubmed publisher
Wong R, Balachandran A, Haaland M, Stoilov P, Cochrane A. Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function. Nucleic Acids Res. 2013;41:9471-83 pubmed publisher
Vivarelli S, Lenzken S, Ruepp M, Ranzini F, Maffioletti A, Alvarez R, et al. Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2. PLoS ONE. 2013;8:e61980 pubmed publisher
Li S, Guo W, Dewey C, Greaser M. Rbm20 regulates titin alternative splicing as a splicing repressor. Nucleic Acids Res. 2013;41:2659-72 pubmed publisher
Schor I, Llères D, Risso G, Pawellek A, Ule J, Lamond A, et al. Perturbation of chromatin structure globally affects localization and recruitment of splicing factors. PLoS ONE. 2012;7:e48084 pubmed publisher
Lin Moshier Y, Sebastian P, Higgins L, Sampson N, Hewitt J, Marchant J. Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling. J Biol Chem. 2013;288:355-67 pubmed publisher
Gout S, Brambilla E, Boudria A, Drissi R, Lantuejoul S, Gazzeri S, et al. Abnormal expression of the pre-mRNA splicing regulators SRSF1, SRSF2, SRPK1 and SRPK2 in non small cell lung carcinoma. PLoS ONE. 2012;7:e46539 pubmed publisher
Pradeepa M, Sutherland H, Ule J, Grimes G, Bickmore W. Psip1/Ledgf p52 binds methylated histone H3K36 and splicing factors and contributes to the regulation of alternative splicing. PLoS Genet. 2012;8:e1002717 pubmed publisher
Sánchez Hernández N, Ruiz L, Sánchez Alvarez M, Montes M, Macias M, Hernández Munain C, et al. The FF4 and FF5 domains of transcription elongation regulator 1 (TCERG1) target proteins to the periphery of speckles. J Biol Chem. 2012;287:17789-800 pubmed publisher
Apostolatos A, Song S, Acosta S, Peart M, Watson J, Bickford P, et al. Insulin promotes neuronal survival via the alternatively spliced protein kinase CδII isoform. J Biol Chem. 2012;287:9299-310 pubmed publisher
Takata H, Nishijima H, Maeshima K, Shibahara K. The integrator complex is required for integrity of Cajal bodies. J Cell Sci. 2012;125:166-75 pubmed publisher
Kress C, Kiêu K, Droineau S, Galio L, Devinoy E. Specific positioning of the casein gene cluster in active nuclear domains in luminal mammary epithelial cells. Chromosome Res. 2011;19:979-97 pubmed publisher
Sun C, van Koningsbruggen S, Long S, Straasheijm K, Klooster R, Jones T, et al. Facioscapulohumeral muscular dystrophy region gene 1 is a dynamic RNA-associated and actin-bundling protein. J Mol Biol. 2011;411:397-416 pubmed publisher
Chang L, Godinez W, Kim I, Tektonidis M, de Lanerolle P, Eils R, et al. Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA. Proc Natl Acad Sci U S A. 2011;108:E136-44 pubmed publisher
Harrigan J, Belotserkovskaya R, Coates J, Dimitrova D, Polo S, Bradshaw C, et al. Replication stress induces 53BP1-containing OPT domains in G1 cells. J Cell Biol. 2011;193:97-108 pubmed publisher
Francis J, Lin W, Rozenblatt Rosen O, Meyerson M. The menin tumor suppressor protein is phosphorylated in response to DNA damage. PLoS ONE. 2011;6:e16119 pubmed publisher
Chen K, Yuan R, Hu C, Hsu C. Amyloid-? peptide alteration of tau exon-10 splicing via the GSK3?-SC35 pathway. Neurobiol Dis. 2010;40:378-85 pubmed publisher
Martinet W, De Meyer I, Cools N, Timmerman V, Bult H, Bosmans J, et al. Cell death-mediated cleavage of the attraction signal p43 in human atherosclerosis: implications for plaque destabilization. Arterioscler Thromb Vasc Biol. 2010;30:1415-22 pubmed publisher
Deutsch M, Ott E, Papior P, Schepers A. The latent origin of replication of Epstein-Barr virus directs viral genomes to active regions of the nucleus. J Virol. 2010;84:2533-46 pubmed publisher
Hancock R, Hadj Sahraoui Y. Isolation of cell nuclei using inert macromolecules to mimic the crowded cytoplasm. PLoS ONE. 2009;4:e7560 pubmed publisher
Uranishi H, Zolotukhin A, Lindtner S, Warming S, Zhang G, Bear J, et al. The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1. J Biol Chem. 2009;284:26106-16 pubmed publisher
Fernandez Martinez J, Vela E, Tora Ponsioen M, Ocaña O, Nieto M, Galceran J. Attenuation of Notch signalling by the Down-syndrome-associated kinase DYRK1A. J Cell Sci. 2009;122:1574-83 pubmed publisher
Russell R, Adams N, Stephens D, Batty E, Jensen K, Freemont P. Segmentation of fluorescence microscopy images for quantitative analysis of cell nuclear architecture. Biophys J. 2009;96:3379-89 pubmed publisher
Fryrear K, Durkin S, Gupta S, Tiedebohl J, Semmes O. Dimerization and a novel Tax speckled structure localization signal are required for Tax nuclear localization. J Virol. 2009;83:5339-52 pubmed publisher
Yung T, Narita T, Komori T, Yamaguchi Y, Handa H. Cellular dynamics of the negative transcription elongation factor NELF. Exp Cell Res. 2009;315:1693-705 pubmed publisher
Murzik U, Hemmerich P, Weidtkamp Peters S, Ulbricht T, Bussen W, Hentschel J, et al. Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11. Mol Biol Cell. 2008;19:2926-35 pubmed publisher
Tang C, Maya Mendoza A, Martin C, Zeng K, Chen S, Feret D, et al. The integrity of a lamin-B1-dependent nucleoskeleton is a fundamental determinant of RNA synthesis in human cells. J Cell Sci. 2008;121:1014-24 pubmed publisher
Inoue A, Tsugawa K, Tokunaga K, Takahashi K, Uni S, Kimura M, et al. S1-1 nuclear domains: characterization and dynamics as a function of transcriptional activity. Biol Cell. 2008;100:523-35 pubmed publisher
Baldin V, Militello M, Thomas Y, Doucet C, Fic W, Boireau S, et al. A novel role for PA28gamma-proteasome in nuclear speckle organization and SR protein trafficking. Mol Biol Cell. 2008;19:1706-16 pubmed publisher
Boireau S, Maiuri P, Basyuk E, De la Mata M, Knezevich A, Pradet Balade B, et al. The transcriptional cycle of HIV-1 in real-time and live cells. J Cell Biol. 2007;179:291-304 pubmed
Smith K, Byron M, Johnson C, Xing Y, Lawrence J. Defining early steps in mRNA transport: mutant mRNA in myotonic dystrophy type I is blocked at entry into SC-35 domains. J Cell Biol. 2007;178:951-64 pubmed
Holt I, Mittal S, Furling D, Butler Browne G, Brook J, Morris G. Defective mRNA in myotonic dystrophy accumulates at the periphery of nuclear splicing speckles. Genes Cells. 2007;12:1035-48 pubmed
Stehle I, Postberg J, Rupprecht S, Cremer T, Jackson D, Lipps H. Establishment and mitotic stability of an extra-chromosomal mammalian replicon. BMC Cell Biol. 2007;8:33 pubmed
Gromoll J, Lahrmann L, Godmann M, Muller T, Michel C, Stamm S, et al. Genomic checkpoints for exon 10 usage in the luteinizing hormone receptor type 1 and type 2. Mol Endocrinol. 2007;21:1984-96 pubmed
Richter K, Nessling M, Lichter P. Experimental evidence for the influence of molecular crowding on nuclear architecture. J Cell Sci. 2007;120:1673-80 pubmed
Kobayakawa S, Miike K, Nakao M, Abe K. Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells. 2007;12:447-60 pubmed
Paronetto M, Achsel T, Massiello A, Chalfant C, Sette C. The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x. J Cell Biol. 2007;176:929-39 pubmed
Raoul C, Buhler E, Sadeghi C, Jacquier A, Aebischer P, Pettmann B, et al. Chronic activation in presymptomatic amyotrophic lateral sclerosis (ALS) mice of a feedback loop involving Fas, Daxx, and FasL. Proc Natl Acad Sci U S A. 2006;103:6007-12 pubmed
Brown J, Leach J, Reittie J, Atzberger A, Lee Prudhoe J, Wood W, et al. Coregulated human globin genes are frequently in spatial proximity when active. J Cell Biol. 2006;172:177-87 pubmed
Barboric M, Kohoutek J, Price J, Blazek D, Price D, Peterlin B. Interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct the inhibition of P-TEFb. EMBO J. 2005;24:4291-303 pubmed
Grm H, Massimi P, Gammoh N, Banks L. Crosstalk between the human papillomavirus E2 transcriptional activator and the E6 oncoprotein. Oncogene. 2005;24:5149-64 pubmed publisher
Tominaga K, Johmura Y, Nishizuka M, Imagawa M. Fad24, a mammalian homolog of Noc3p, is a positive regulator in adipocyte differentiation. J Cell Sci. 2004;117:6217-26 pubmed
Smolen G, Vassileva M, Wells J, Matunis M, Haber D. SUMO-1 modification of the Wilms' tumor suppressor WT1. Cancer Res. 2004;64:7846-51 pubmed
Tam R, Smith K, Lawrence J. The 4q subtelomere harboring the FSHD locus is specifically anchored with peripheral heterochromatin unlike most human telomeres. J Cell Biol. 2004;167:269-79 pubmed
Delhaye S, van Pesch V, Michiels T. The leader protein of Theiler's virus interferes with nucleocytoplasmic trafficking of cellular proteins. J Virol. 2004;78:4357-62 pubmed
Herrmann A, Sommer U, Pranada A, Giese B, Küster A, Haan S, et al. STAT3 is enriched in nuclear bodies. J Cell Sci. 2004;117:339-49 pubmed
Shopland L, Johnson C, Byron M, McNeil J, Lawrence J. Clustering of multiple specific genes and gene-rich R-bands around SC-35 domains: evidence for local euchromatic neighborhoods. J Cell Biol. 2003;162:981-90 pubmed
Piwien Pilipuk G, Galigniana M, Schwartz J. Subnuclear localization of C/EBP beta is regulated by growth hormone and dependent on MAPK. J Biol Chem. 2003;278:35668-77 pubmed
Kamachi M, Le T, Kim S, Geiger M, Anderson P, Utz P. Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway. J Exp Med. 2002;196:1213-25 pubmed
Leung A, Lamond A. In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles. J Cell Biol. 2002;157:615-29 pubmed
Nayler O, Hartmann A, Stamm S. The ER repeat protein YT521-B localizes to a novel subnuclear compartment. J Cell Biol. 2000;150:949-62 pubmed
Johnson C, Primorac D, McKinstry M, McNeil J, Rowe D, Lawrence J. Tracking COL1A1 RNA in osteogenesis imperfecta. splice-defective transcripts initiate transport from the gene but are retained within the SC35 domain. J Cell Biol. 2000;150:417-32 pubmed
Kockx M, Muhring J, Knaapen M, De Meyer G. RNA synthesis and splicing interferes with DNA in situ end labeling techniques used to detect apoptosis. Am J Pathol. 1998;152:885-8 pubmed
Jiménez García L, Spector D. In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell. 1993;73:47-59 pubmed
Gui J, Lane W, Fu X. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature. 1994;369:678-82 pubmed
Fu X, Maniatis T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature. 1990;343:437-41 pubmed
Spector D, Fu X, Maniatis T. Associations between distinct pre-mRNA splicing components and the cell nucleus. EMBO J. 1991;10:3467-81 pubmed
Wang J, Cao L, Wang Y, Pederson T. Localization of pre-messenger RNA at discrete nuclear sites. Proc Natl Acad Sci U S A. 1991;88:7391-5 pubmed
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Fu X, Maniatis T. The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site. Proc Natl Acad Sci U S A. 1992;89:1725-9 pubmed
Fu X, Mayeda A, Maniatis T, Krainer A. General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5' and 3' splice site selection. Proc Natl Acad Sci U S A. 1992;89:11224-8 pubmed
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product information
Product No. :
S4045
Product Name :
Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
SC-35
Applications/Description :
Suitable for electron microscopy; indirect immunofluorescence: 1:2,000 using cultured human fibroblasts; indirect ELISA; immunoprecipitation (IP); immunocytochemistry
Species Reactivity :
rat, newt, frog, human, Drosophila
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA