product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse
catalog :
P7965
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F4
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - frozen section
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:2000; loading ...; fig 2
Lopes Rodrigues V, Di Luca A, Mleczko J, Meleady P, Henry M, Pesic M, et al. Identification of the metabolic alterations associated with the multidrug resistant phenotype in cancer and their intercellular transfer mediated by extracellular vesicles. Sci Rep. 2017;7:44541 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 3a
Seebacher N, Richardson D, Jansson P. A mechanism for overcoming P-glycoprotein-mediated drug resistance: novel combination therapy that releases stored doxorubicin from lysosomes via lysosomal permeabilization using Dp44mT or DpC. Cell Death Dis. 2016;7:e2510 pubmed publisher
  • immunohistochemistry; human
  • western blot; human
Jansson P, Yamagishi T, Arvind A, Seebacher N, Gutierrez E, Stacy A, et al. Di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) overcomes multidrug resistance by a novel mechanism involving the hijacking of lysosomal P-glycoprotein (Pgp). J Biol Chem. 2015;290:9588-603 pubmed publisher
Katt M, Mayo L, Ellis S, Mahairaki V, Rothstein J, Cheng L, et al. The role of mutations associated with familial neurodegenerative disorders on blood-brain barrier function in an iPSC model. Fluids Barriers CNS. 2019;16:20 pubmed publisher
Ji N, Yang Y, Cai C, Wang J, Lei Z, Wu Z, et al. Midostaurin Reverses ABCB1-Mediated Multidrug Resistance, an in vitro Study. Front Oncol. 2019;9:514 pubmed publisher
De Vera A, Gupta P, Lei Z, Liao D, Narayanan S, Teng Q, et al. Immuno-oncology agent IPI-549 is a modulator of P-glycoprotein (P-gp, MDR1, ABCB1)-mediated multidrug resistance (MDR) in cancer: In vitro and in vivo. Cancer Lett. 2019;442:91-103 pubmed publisher
Ji N, Yang Y, Cai C, Lei Z, Wang J, Gupta P, et al. VS-4718 Antagonizes Multidrug Resistance in ABCB1- and ABCG2-Overexpressing Cancer Cells by Inhibiting the Efflux Function of ABC Transporters. Front Pharmacol. 2018;9:1236 pubmed publisher
Wang H, Gao Z, Liu X, Agarwal P, Zhao S, Conroy D, et al. Targeted production of reactive oxygen species in mitochondria to overcome cancer drug resistance. Nat Commun. 2018;9:562 pubmed publisher
Al Akra L, Bae D, Sahni S, Huang M, Park K, Lane D, et al. Tumor stressors induce two mechanisms of intracellular P-glycoprotein-mediated resistance that are overcome by lysosomal-targeted thiosemicarbazones. J Biol Chem. 2018;293:3562-3587 pubmed publisher
Inci F, Celik U, Turken B, Özer H, Kok F. Construction of P-glycoprotein incorporated tethered lipid bilayer membranes. Biochem Biophys Rep. 2015;2:115-122 pubmed publisher
Wang C, Huang C, Chou P, Chang Y, Wei S, Guengerich F, et al. 7-ketocholesterol and 27-hydroxycholesterol decreased doxorubicin sensitivity in breast cancer cells: estrogenic activity and mTOR pathway. Oncotarget. 2017;8:66033-66050 pubmed publisher
Lopes Rodrigues V, Oliveira A, Correia da Silva M, Pinto M, Lima R, Sousa E, et al. A novel curcumin derivative which inhibits P-glycoprotein, arrests cell cycle and induces apoptosis in multidrug resistance cells. Bioorg Med Chem. 2017;25:581-596 pubmed publisher
Ma J, Zhang Y, Tang K, Zhang H, Yin X, Li Y, et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res. 2016;26:713-27 pubmed publisher
Zhang Y, Zhang G, Wang Y, Patel B, Talele T, Yang D, et al. Bafetinib (INNO-406) reverses multidrug resistance by inhibiting the efflux function of ABCB1 and ABCG2 transporters. Sci Rep. 2016;6:25694 pubmed publisher
Wang Y, Huang Y, Anreddy N, Zhang G, Zhang Y, Xie M, et al. Tea nanoparticle, a safe and biocompatible nanocarrier, greatly potentiates the anticancer activity of doxorubicin. Oncotarget. 2016;7:5877-91 pubmed publisher
Seebacher N, Lane D, Jansson P, Richardson D. Glucose Modulation Induces Lysosome Formation and Increases Lysosomotropic Drug Sequestration via the P-Glycoprotein Drug Transporter. J Biol Chem. 2016;291:3796-820 pubmed publisher
Giuliano S, Cormerais Y, Dufies M, Grepin R, Colosetti P, Belaid A, et al. Resistance to sunitinib in renal clear cell carcinoma results from sequestration in lysosomes and inhibition of the autophagic flux. Autophagy. 2015;11:1891-904 pubmed publisher
Zhang Y, Zhang H, Zhang G, Wang Y, Kathawala R, Si R, et al. Semi-synthetic ocotillol analogues as selective ABCB1-mediated drug resistance reversal agents. Oncotarget. 2015;6:24277-90 pubmed
Ween M, Armstrong M, Oehler M, Ricciardelli C. The role of ABC transporters in ovarian cancer progression and chemoresistance. Crit Rev Oncol Hematol. 2015;96:220-56 pubmed publisher
Seebacher N, Richardson D, Jansson P. Glucose modulation induces reactive oxygen species and increases P-glycoprotein-mediated multidrug resistance to chemotherapeutics. Br J Pharmacol. 2015;172:2557-72 pubmed publisher
Kadova Z, Dolezelova E, Cermanova J, Hroch M, Laho T, Muchova L, et al. IL-1 receptor blockade alleviates endotoxin-mediated impairment of renal drug excretory functions in rats. Am J Physiol Renal Physiol. 2015;308:F388-99 pubmed publisher
Wang Y, Kathawala R, Zhang Y, Patel A, Kumar P, Shukla S, et al. Motesanib (AMG706), a potent multikinase inhibitor, antagonizes multidrug resistance by inhibiting the efflux activity of the ABCB1. Biochem Pharmacol. 2014;90:367-78 pubmed publisher
Dessilly G, Elens L, Panin N, Capron A, Decottignies A, Demoulin J, et al. ABCB1 1199G>A genetic polymorphism (Rs2229109) influences the intracellular accumulation of tacrolimus in HEK293 and K562 recombinant cell lines. PLoS ONE. 2014;9:e91555 pubmed publisher
Ma Q, Zhang Y, Shi J, Li G. Clinical significance of P-glycoprotein and glutathione S-transferase ? expression in gallbladder carcinoma. Exp Ther Med. 2014;7:635-639 pubmed
Yamagishi T, Sahni S, Sharp D, Arvind A, Jansson P, Richardson D. P-glycoprotein mediates drug resistance via a novel mechanism involving lysosomal sequestration. J Biol Chem. 2013;288:31761-71 pubmed publisher
Yosef N, Ubogu E. An immortalized human blood-nerve barrier endothelial cell line for in vitro permeability studies. Cell Mol Neurobiol. 2013;33:175-86 pubmed publisher
Su C. Tanshinone IIA potentiates the efficacy of 5-FU in Colo205 colon cancer cells in vivo through downregulation of P-gp and LC3-II. Exp Ther Med. 2012;3:555-559 pubmed
Ferreira P, Ruela de Sousa R, Queiroz K, Souza A, Milani R, Pilli R, et al. Knocking down low molecular weight protein tyrosine phosphatase (LMW-PTP) reverts chemoresistance through inactivation of Src and Bcr-Abl proteins. PLoS ONE. 2012;7:e44312 pubmed publisher
Mason C, Buhimschi I, Buhimschi C, Dong Y, Weiner C, Swaan P. ATP-binding cassette transporter expression in human placenta as a function of pregnancy condition. Drug Metab Dispos. 2011;39:1000-7 pubmed publisher
Abraham I, Jain S, Wu C, Khanfar M, Kuang Y, Dai C, et al. Marine sponge-derived sipholane triterpenoids reverse P-glycoprotein (ABCB1)-mediated multidrug resistance in cancer cells. Biochem Pharmacol. 2010;80:1497-506 pubmed publisher
Shen T, Kuang Y, Ashby C, Lei Y, Chen A, Zhou Y, et al. Imatinib and nilotinib reverse multidrug resistance in cancer cells by inhibiting the efflux activity of the MRP7 (ABCC10). PLoS ONE. 2009;4:e7520 pubmed publisher
Kuang Y, Shen T, Chen X, Sodani K, Hopper Borge E, Tiwari A, et al. Lapatinib and erlotinib are potent reversal agents for MRP7 (ABCC10)-mediated multidrug resistance. Biochem Pharmacol. 2010;79:154-61 pubmed publisher
Zhou Y, Hopper Borge E, Shen T, Huang X, Shi Z, Kuang Y, et al. Cepharanthine is a potent reversal agent for MRP7(ABCC10)-mediated multidrug resistance. Biochem Pharmacol. 2009;77:993-1001 pubmed publisher
Shi Z, Tiwari A, Shukla S, Robey R, Kim I, Parmar S, et al. Inhibiting the function of ABCB1 and ABCG2 by the EGFR tyrosine kinase inhibitor AG1478. Biochem Pharmacol. 2009;77:781-93 pubmed publisher
Lemaire S, Van Bambeke F, Mingeot Leclercq M, Tulkens P. Modulation of the cellular accumulation and intracellular activity of daptomycin towards phagocytized Staphylococcus aureus by the P-glycoprotein (MDR1) efflux transporter in human THP-1 macrophages and madin-darby canine kidney cells. Antimicrob Agents Chemother. 2007;51:2748-57 pubmed
Azzariti A, Colabufo N, Berardi F, Porcelli L, Niso M, Simone G, et al. Cyclohexylpiperazine derivative PB28, a sigma2 agonist and sigma1 antagonist receptor, inhibits cell growth, modulates P-glycoprotein, and synergizes with anthracyclines in breast cancer. Mol Cancer Ther. 2006;5:1807-16 pubmed
Leitch G, Scanlon M, Shaw A, Visvesvara G. Role of P glycoprotein in the course and treatment of Encephalitozoon microsporidiosis. Antimicrob Agents Chemother. 2001;45:73-8 pubmed
Chu T, Lin T, Kawinski E. Detection of soluble P-glycoprotein in culture media and extracellular fluids. Biochem Biophys Res Commun. 1994;203:506-12 pubmed
Chu T, Kawinski E, Lin T. Characterization of a new monoclonal antibody F4 detecting cell surface epitope and P-glycoprotein in drug-resistant human tumor cell lines. Hybridoma. 1993;12:417-29 pubmed
product information
Product No. :
P7965
Product Name :
Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
F4
Applications/Description :
Suitable for immunocytochemistry; western blot; radioimmunoassay using cell-surface RIA; indirect ELISA; immunoprecipitation (IP); immunohistochemistry (frozen sections);
Species Reactivity :
hamster, human
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA