product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Phosphothreonine antibody produced in mouse
catalog :
P6623
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
PTR-8
reactivity :
human, mouse
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
citations: 11
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human
Michard C, Sperandio D, Baïlo N, Pizarro Cerdá J, LeClaire L, Chadeau Argaud E, et al. The Legionella Kinase LegK2 Targets the ARP2/3 Complex To Inhibit Actin Nucleation on Phagosomes and Allow Bacterial Evasion of the Late Endocytic Pathway. MBio. 2015;6:e00354-15 pubmed publisher
  • western blot; mouse
Miyagishima H, Isono K, Fujimura Y, Iyo M, Takihara Y, Masumoto H, et al. Dissociation of mammalian Polycomb-group proteins, Ring1B and Rae28/Ph1, from the chromatin correlates with configuration changes of the chromatin in mitotic and meiotic prophase. Histochem Cell Biol. 2003;120:111-9 pubmed
Woodford M, Hughes M, Sager R, Backe S, Baker Williams A, Bratslavsky M, et al. Mutation of the co-chaperone Tsc1 in bladder cancer diminishes Hsp90 acetylation and reduces drug sensitivity and selectivity. Oncotarget. 2019;10:5824-5834 pubmed publisher
Chi T, Horbach T, Götz C, Kietzmann T, Dimova E. Cyclin-Dependent Kinase 5 (CDK5)-Mediated Phosphorylation of Upstream Stimulatory Factor 2 (USF2) Contributes to Carcinogenesis. Cancers (Basel). 2019;11: pubmed publisher
Sager R, Woodford M, Backe S, Makedon A, Baker Williams A, DiGregorio B, et al. Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90. Cell Rep. 2019;26:1344-1356.e5 pubmed publisher
Nixon B, Johnston S, Skerrett Byrne D, Anderson A, Stanger S, Bromfield E, et al. Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals. Mol Cell Proteomics. 2019;18:S59-S76 pubmed publisher
Sager R, Woodford M, Neckers L, Mollapour M. Detecting Posttranslational Modifications of Hsp90. Methods Mol Biol. 2018;1709:209-219 pubmed publisher
Nixon B, Anderson A, Smith N, McLeod R, Johnston S. The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage. Proc Biol Sci. 2016;283: pubmed publisher
Lord T, Aitken R. Fertilization stimulates 8-hydroxy-2'-deoxyguanosine repair and antioxidant activity to prevent mutagenesis in the embryo. Dev Biol. 2015;406:1-13 pubmed publisher
Bardales J, Hellman U, Villamarin J. Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis. FEBS J. 2008;275:4479-89 pubmed publisher
Kim H, Han J, Park J, Oh M, James S, Chang S, et al. Delta-catenin-induced dendritic morphogenesis. An essential role of p190RhoGEF interaction through Akt1-mediated phosphorylation. J Biol Chem. 2008;283:977-87 pubmed
product information
Product No. :
P6623
Product Name :
Monoclonal Anti-Phosphothreonine antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
PTR-8
Applications/Description :
Suitable for dot blot; western blot: 5-10 μg/mL using A431 cell extracts; microarray; indirect ELISA: 0.5-1 μg/mL; immunoprecipitation (IP); immunocytochemistry
Isotype :
IgG2b
Concentration :
~2.5 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA