product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Anti-ORAI1 antibody produced in rabbit
catalog :
O8264
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunoprecipitation
more info or order :
citations: 28
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 2a
Diez Bello R, Jardin I, Salido G, Rosado J. Orai1 and Orai2 mediate store-operated calcium entry that regulates HL60 cell migration and FAK phosphorylation. Biochim Biophys Acta Mol Cell Res. 2017;1864:1064-1070 pubmed publisher
  • immunoprecipitation; human; fig s2
  • western blot; human; fig s1
Albarrán L, Lopez J, Amor N, Martin Cano F, Berna Erro A, Smani T, et al. Dynamic interaction of SARAF with STIM1 and Orai1 to modulate store-operated calcium entry. Sci Rep. 2016;6:24452 pubmed publisher
Luan H, Bielecki T, Mohapatra B, Islam N, Mushtaq I, Bhat A, et al. EHD2 overexpression promotes tumorigenesis and metastasis in triple-negative breast cancer by regulating store-operated calcium entry. elife. 2023;12: pubmed publisher
Sanchez Collado J, Lopez J, Jardin I, Berna Erro A, Camello P, Cantonero C, et al. Orai1α, but not Orai1β, co-localizes with TRPC1 and is required for its plasma membrane location and activation in HeLa cells. Cell Mol Life Sci. 2022;79:33 pubmed publisher
Sanchez Collado J, Lopez J, Cantonero C, Jardin I, Regodón S, Redondo P, et al. Orai2 Modulates Store-Operated Ca2+ Entry and Cell Cycle Progression in Breast Cancer Cells. Cancers (Basel). 2021;14: pubmed publisher
Zomot E, Achildiev Cohen H, Dagan I, Militsin R, Palty R. Bidirectional regulation of calcium release-activated calcium (CRAC) channel by SARAF. J Cell Biol. 2021;220: pubmed publisher
Cheng P, Tian X, Tang W, Cheng J, Bao J, Wang H, et al. Direct control of store-operated calcium channels by ultrafast laser. Cell Res. 2021;: pubmed publisher
Yue L, Wang L, Du Y, Zhang W, Hamada K, Matsumoto Y, et al. Type 3 Inositol 1,4,5-Trisphosphate Receptor is a Crucial Regulator of Calcium Dynamics Mediated by Endoplasmic Reticulum in HEK Cells. Cells. 2020;9: pubmed publisher
Alansary D, Peckys D, Niemeyer B, de Jonge N. Detecting single ORAI1 proteins within the plasma membrane reveals higher order channel complexes. J Cell Sci. 2019;: pubmed publisher
Sanchez Collado J, Lopez J, Jardin I, Camello P, Falcon D, Regodón S, et al. Adenylyl Cyclase Type 8 Overexpression Impairs Phosphorylation-Dependent Orai1 Inactivation and Promotes Migration in MDA-MB-231 Breast Cancer Cells. Cancers (Basel). 2019;11: pubmed publisher
Yeh Y, Lin Y, Kramer H, Parekh A. Single nucleotide polymorphisms in Orai1 associated with atopic dermatitis inhibit protein turnover, decrease calcium entry and disrupt calcium-dependent gene expression. Hum Mol Genet. 2019;: pubmed publisher
Huang L, Ma R, Lin T, Chaudhari S, Shotorbani P, Yang L, et al. Glucagon-like peptide-1 receptor pathway inhibits extracellular matrix production by mesangial cells through store-operated Ca2+ channel. Exp Biol Med (Maywood). 2019;244:1193-1201 pubmed publisher
Zhang X, Pathak T, Yoast R, Emrich S, Xin P, Nwokonko R, et al. A calcium/cAMP signaling loop at the ORAI1 mouth drives channel inactivation to shape NFAT induction. Nat Commun. 2019;10:1971 pubmed publisher
Lu F, Sun J, Zheng Q, Li J, Hu Y, Yu P, et al. Imaging elemental events of store-operated Ca2+ entry in invading cancer cells with plasmalemmal targeted sensors. J Cell Sci. 2019;: pubmed publisher
Albarrán L, Lopez J, Jardin I, Sanchez Collado J, Berna Erro A, Smani T, et al. EFHB is a Novel Cytosolic Ca2+ Sensor That Modulates STIM1-SARAF Interaction. Cell Physiol Biochem. 2018;51:1164-1178 pubmed publisher
Czeredys M, Vigont V, Boeva V, Mikoshiba K, Kaznacheyeva E, Kuznicki J. Huntingtin-Associated Protein 1A Regulates Store-Operated Calcium Entry in Medium Spiny Neurons From Transgenic YAC128 Mice, a Model of Huntington's Disease. Front Cell Neurosci. 2018;12:381 pubmed publisher
Jardin I, Diez Bello R, Lopez J, Redondo P, Salido G, Smani T, et al. TRPC6 Channels Are Required for Proliferation, Migration and Invasion of Breast Cancer Cell Lines by Modulation of Orai1 and Orai3 Surface Exposure. Cancers (Basel). 2018;10: pubmed publisher
Wang W, Ren Y, Wang L, Zhao W, Dong X, Pan J, et al. Orai1 and Stim1 Mediate the Majority of Store-Operated Calcium Entry in Multiple Myeloma and Have Strong Implications for Adverse Prognosis. Cell Physiol Biochem. 2018;48:2273-2285 pubmed publisher
Liu Z, Wei Y, Zhang L, Yee P, Johnson M, Zhang X, et al. Induction of store-operated calcium entry (SOCE) suppresses glioblastoma growth by inhibiting the Hippo pathway transcriptional coactivators YAP/TAZ. Oncogene. 2019;38:120-139 pubmed publisher
Jiang H, Zou S, Chaudhari S, Ma R. Short-term high-glucose treatment decreased abundance of Orai1 protein through posttranslational mechanisms in rat mesangial cells. Am J Physiol Renal Physiol. 2018;314:F855-F863 pubmed publisher
Nunes Hasler P, Maschalidi S, Lippens C, Castelbou C, Bouvet S, Guido D, et al. STIM1 promotes migration, phagosomal maturation and antigen cross-presentation in dendritic cells. Nat Commun. 2017;8:1852 pubmed publisher
Chaudhari S, Li W, Wang Y, Jiang H, Ma Y, Davis M, et al. Store-operated calcium entry suppressed the TGF-β1/Smad3 signaling pathway in glomerular mesangial cells. Am J Physiol Renal Physiol. 2017;313:F729-F739 pubmed publisher
Zheng C, Lo C, Meng Z, Li Z, Zhong M, Zhang P, et al. Gastrodin Inhibits Store-Operated Ca2+ Entry and Alleviates Cardiac Hypertrophy. Front Pharmacol. 2017;8:222 pubmed publisher
van Vliet A, Giordano F, Gerlo S, Segura I, Van Eygen S, Molenberghs G, et al. The ER Stress Sensor PERK Coordinates ER-Plasma Membrane Contact Site Formation through Interaction with Filamin-A and F-Actin Remodeling. Mol Cell. 2017;65:885-899.e6 pubmed publisher
Sabourin J, Bartoli F, Antigny F, Gomez A, Benitah J. Transient Receptor Potential Canonical (TRPC)/Orai1-dependent Store-operated Ca2+ Channels: NEW TARGETS OF ALDOSTERONE IN CARDIOMYOCYTES. J Biol Chem. 2016;291:13394-409 pubmed publisher
Sabourin J, Le Gal L, Saurwein L, Haefliger J, Raddatz E, Allagnat F. Store-operated Ca2+ Entry Mediated by Orai1 and TRPC1 Participates to Insulin Secretion in Rat β-Cells. J Biol Chem. 2015;290:30530-9 pubmed publisher
Yeh Y, Parekh A. Distinct structural domains of caveolin-1 independently regulate Ca2+ release-activated Ca2+ channels and Ca2+ microdomain-dependent gene expression. Mol Cell Biol. 2015;35:1341-9 pubmed publisher
Endo Y, Noguchi S, Hara Y, Hayashi Y, Motomura K, Miyatake S, et al. Dominant mutations in ORAI1 cause tubular aggregate myopathy with hypocalcemia via constitutive activation of store-operated Ca²⁺ channels. Hum Mol Genet. 2015;24:637-48 pubmed publisher
product information
Product No. :
O8264
Product Name :
Anti-ORAI1 antibody produced in rabbit
Host :
rabbit
Conjugate :
unconjugated
Antibody Type :
polyclonal
Applications/Description :
Suitable for western blot: 1-2 μg/mL using whole extract of human HEK-293T cells expressing ORAI1
Species Reactivity :
human
Concentration :
~1.0 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA