product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Opsin antibody produced in mouse
catalog :
O4886
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RET-P1
reactivity :
human, mouse, rat, bovine, Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, radioimmunoassay, immunohistochemistry - frozen section
more info or order :
citations: 44
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig s3a
Matsuyama T, Tu H, Sun J, Hashiguchi T, Akiba R, Sho J, et al. Genetically engineered stem cell-derived retinal grafts for improved retinal reconstruction after transplantation. iScience. 2021;24:102866 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 3a
Völkner M, Kurth T, Schor J, Ebner L, Bardtke L, Kavak C, et al. Mouse Retinal Organoid Growth and Maintenance in Longer-Term Culture. Front Cell Dev Biol. 2021;9:645704 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:5000; loading ...; fig 2b
Nomura Komoike K, Saitoh F, Fujieda H. Phosphatidylserine recognition and Rac1 activation are required for Müller glia proliferation, gliosis and phagocytosis after retinal injury. Sci Rep. 2020;10:1488 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 1g
Stefanov A, Novelli E, Strettoi E. Inner retinal preservation in the photoinducible I307N rhodopsin mutant mouse, a model of autosomal dominant retinitis pigmentosa. J Comp Neurol. 2020;528:1502-1522 pubmed publisher
  • immunocytochemistry; human; fig 3c
Zhu J, Cifuentes H, Reynolds J, Lamba D. Immunosuppression via Loss of IL2rγ Enhances Long-Term Functional Integration of hESC-Derived Photoreceptors in the Mouse Retina. Cell Stem Cell. 2017;20:374-384.e5 pubmed publisher
  • immunohistochemistry; Xenopus laevis; 1:500; loading ...; tbl 1
Messina A, Bridi S, Bozza A, Bozzi Y, Baudet M, Casarosa S. Noggin 1 overexpression in retinal progenitors affects bipolar cell generation. Int J Dev Biol. 2016;60:151-7 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 2
Boggio E, Pancrazi L, Gennaro M, Lo Rizzo C, Mari F, Meloni I, et al. Visual impairment in FOXG1-mutated individuals and mice. Neuroscience. 2016;324:496-508 pubmed publisher
  • immunohistochemistry - frozen section; Xenopus laevis; 1:1000; fig 3
Roque C, Wong H, Lin J, Holt C. Tumor protein Tctp regulates axon development in the embryonic visual system. Development. 2016;143:1134-48 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:10,000; fig 5a
Watanabe S, Sanuki R, Sugita Y, Imai W, Yamazaki R, Kozuka T, et al. Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity. J Neurosci. 2015;35:8004-20 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000
Nishiguchi K, Carvalho L, Rizzi M, Powell K, Holthaus S, Azam S, et al. Gene therapy restores vision in rd1 mice after removal of a confounding mutation in Gpr179. Nat Commun. 2015;6:6006 pubmed publisher
Trotta M, Pieretti G, Petrillo F, Alessio N, Hermenean A, Maisto R, et al. Resolvin D1 reduces mitochondrial damage to photoreceptors of primary retinal cells exposed to high glucose. J Cell Physiol. 2020;235:4256-4267 pubmed publisher
Guadagni V, Biagioni M, Novelli E, Aretini P, Mazzanti C, Strettoi E. Rescuing cones and daylight vision in retinitis pigmentosa mice. FASEB J. 2019;33:10177-10192 pubmed publisher
Wan A, Place E, Pierce E, Comander J. Characterizing variants of unknown significance in rhodopsin: A functional genomics approach. Hum Mutat. 2019;: pubmed publisher
Falk N, Kessler K, Schramm S, Boldt K, Becirovic E, Michalakis S, et al. Functional analyses of Pericentrin and Syne-2 interaction in ciliogenesis. J Cell Sci. 2018;131: pubmed publisher
Ruzycki P, Linne C, Hennig A, Chen S. Crx-L253X Mutation Produces Dominant Photoreceptor Defects in TVRM65 Mice. Invest Ophthalmol Vis Sci. 2017;58:4644-4653 pubmed publisher
Gargini C, Novelli E, Piano I, Biagioni M, Strettoi E. Pattern of retinal morphological and functional decay in a light-inducible, rhodopsin mutant mouse. Sci Rep. 2017;7:5730 pubmed publisher
Bhattacharyya N, Darren B, Schott R, Tropepe V, Chang B. Cone-like rhodopsin expressed in the all-cone retina of the colubrid pine snake as a potential adaptation to diurnality. J Exp Biol. 2017;220:2418-2425 pubmed publisher
Lu S, Sung T, Lin N, Abraham R, Jessen B. Lysosomal adaptation: How cells respond to lysosomotropic compounds. PLoS ONE. 2017;12:e0173771 pubmed publisher
Lv J, Zhou G, Chen X, Chen H, Wu K, Xiang L, et al. Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9. Sci Rep. 2017;7:43062 pubmed publisher
Tao C, Zhang X. Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis. Cell Rep. 2016;17:1832-1844 pubmed publisher
Guadagni V, Cerri C, Piano I, Novelli E, Gargini C, Fiorentini C, et al. The bacterial toxin CNF1 as a tool to induce retinal degeneration reminiscent of retinitis pigmentosa. Sci Rep. 2016;6:35919 pubmed publisher
Ul Quraish R, Sudou N, Nomura Komoike K, Sato F, Fujieda H. p27KIP1 loss promotes proliferation and phagocytosis but prevents epithelial-mesenchymal transition in RPE cells after photoreceptor damage. Mol Vis. 2016;22:1103-1121 pubmed
Barone I, Novelli E, Strettoi E. Long-term preservation of cone photoreceptors and visual acuity in rd10 mutant mice exposed to continuous environmental enrichment. Mol Vis. 2014;20:1545-56 pubmed
Daiger S, Sullivan L, Bowne S. Genes and mutations causing retinitis pigmentosa. Clin Genet. 2013;84:132-41 pubmed publisher
Watanabe S, Sanuki R, Ueno S, Koyasu T, Hasegawa T, Furukawa T. Tropisms of AAV for subretinal delivery to the neonatal mouse retina and its application for in vivo rescue of developmental photoreceptor disorders. PLoS ONE. 2013;8:e54146 pubmed publisher
Barone I, Novelli E, Piano I, Gargini C, Strettoi E. Environmental enrichment extends photoreceptor survival and visual function in a mouse model of retinitis pigmentosa. PLoS ONE. 2012;7:e50726 pubmed publisher
Woo T, Li S, Lai W, Wong D, Lo A. Neuroprotective effects of lutein in a rat model of retinal detachment. Graefes Arch Clin Exp Ophthalmol. 2013;251:41-51 pubmed publisher
Czekaj M, Haas J, Gebhardt M, MULLER REICHERT T, Humphries P, Farrar J, et al. In vitro expanded stem cells from the developing retina fail to generate photoreceptors but differentiate into myelinating oligodendrocytes. PLoS ONE. 2012;7:e41798 pubmed publisher
Mühlhans J, Brandstätter J, Giessl A. The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors. PLoS ONE. 2011;6:e26496 pubmed publisher
Kitareewan S, Blumen S, Sekula D, Bissonnette R, Lamph W, Cui Q, et al. G0S2 is an all-trans-retinoic acid target gene. Int J Oncol. 2008;33:397-404 pubmed
Hu Y, Zhang T, Wu J, Li Y, Lu X, Qian F, et al. Autologous transplantation of RPE with partial-thickness choroid after mechanical debridement of Bruch membrane in the rabbit. Invest Ophthalmol Vis Sci. 2008;49:3185-92 pubmed publisher
Li S, Tay D, Shu S, Bao X, Wu Y, Wang X, et al. Endothelial nitric oxide synthase is expressed in amacrine cells of developing human retinas. Invest Ophthalmol Vis Sci. 2006;47:2141-9 pubmed
Fekete D, Barnstable C. The subcellular localization of rat photoreceptor-specific antigens. J Neurocytol. 1983;12:785-803 pubmed
Barnstable C. Monoclonal antibodies which recognize different cell types in the rat retina. Nature. 1980;286:231-5 pubmed
Hicks D, Barnstable C. Lectin and antibody labelling of developing rat photoreceptor cells: an electron microscope immunocytochemical study. J Neurocytol. 1986;15:219-30 pubmed
Hicks D, Barnstable C. Different rhodopsin monoclonal antibodies reveal different binding patterns on developing and adult rat retina. J Histochem Cytochem. 1987;35:1317-28 pubmed
Akagawa K, Barnstable C. Identification and characterization of cell types in monolayer cultures of rat retina using monoclonal antibodies. Brain Res. 1986;383:110-20 pubmed
Barnstable C. Immunological studies of the diversity and development of the mammalian visual system. Immunol Rev. 1987;100:47-78 pubmed
Silver R, Witkovsky P, Horvath P, Alones V, Barnstable C, Lehman M. Coexpression of opsin- and VIP-like-immunoreactivity in CSF-contacting neurons of the avian brain. Cell Tissue Res. 1988;253:189-98 pubmed
Treisman J, Morabito M, Barnstable C. Opsin expression in the rat retina is developmentally regulated by transcriptional activation. Mol Cell Biol. 1988;8:1570-9 pubmed
Silverman M, Hughes S. Photoreceptor transplantation in inherited and environmentally induced retinal degeneration: anatomy, immunohistochemistry and function. Prog Clin Biol Res. 1989;314:687-704 pubmed
Hargrave P, Adamus G, Arendt A, McDowell J, Wang J, Szaby A, et al. Rhodopsin's amino terminus is a principal antigenic site. Exp Eye Res. 1986;42:363-73 pubmed
Lillien L, Cepko C. Control of proliferation in the retina: temporal changes in responsiveness to FGF and TGF alpha. Development. 1992;115:253-66 pubmed
Watanabe T, Raff M. Diffusible rod-promoting signals in the developing rat retina. Development. 1992;114:899-906 pubmed
product information
Product No. :
O4886
Product Name :
Monoclonal Anti-Opsin antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
RET-P1
Applications/Description :
Suitable for electron microscopy; western blot; radioimmunoassay; indirect ELISA; immunohistochemistry (frozen sections): 1:10,000 using frozen sections of rat eye; immunocytochemistry using cultured cells
Species Reactivity :
goldfish, bovine, duck, amphibian, rabbit, rat, turtle, tiger salamander, dove, quail, mouse, human
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA