product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Neurofilament 68 antibody produced in mouse
catalog :
N5139
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
NR4
reactivity :
human, mouse, rat, chicken
application :
western blot, immunohistochemistry, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; loading ...; fig 1b
Rutherford N, Brooks M, Riffe C, Gorion K, Howard J, Dhillon J, et al. Prion-like transmission of α-synuclein pathology in the context of an NFL null background. Neurosci Lett. 2017;661:114-120 pubmed publisher
  • immunohistochemistry; rat; 1:1000; loading ...; tbl 2
  • western blot; rat; 1:1000; loading ...; tbl 2
Han Q, Lin Q, Huang P, Chen M, Hu X, Fu H, et al. Microglia-derived IL-1? contributes to axon development disorders and synaptic deficit through p38-MAPK signal pathway in septic neonatal rats. J Neuroinflammation. 2017;14:52 pubmed publisher
  • western blot; human; 1:500; fig ev3
Fernández Santiago R, Carballo Carbajal I, Castellano G, Torrent R, Richaud Y, Sánchez Danés A, et al. Aberrant epigenome in iPSC-derived dopaminergic neurons from Parkinson's disease patients. EMBO Mol Med. 2015;7:1529-46 pubmed publisher
  • immunohistochemistry; mouse
  • western blot; mouse
Rao M, McBrayer M, Campbell J, Kumar A, Hashim A, Sershen H, et al. Specific calpain inhibition by calpastatin prevents tauopathy and neurodegeneration and restores normal lifespan in tau P301L mice. J Neurosci. 2014;34:9222-34 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400
  • immunohistochemistry - frozen section; rat
Karnas D, Hicks D, Mordel J, Pevet P, Meissl H. Intrinsic photosensitive retinal ganglion cells in the diurnal rodent, Arvicanthis ansorgei. PLoS ONE. 2013;8:e73343 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400
Karnas D, Mordel J, Bonnet D, Pevet P, Hicks D, Meissl H. Heterogeneity of intrinsically photosensitive retinal ganglion cells in the mouse revealed by molecular phenotyping. J Comp Neurol. 2013;521:912-32 pubmed publisher
  • western blot; mouse
Chen Q, Peto C, Shelton G, Mizisin A, Sawchenko P, Schubert D. Loss of modifier of cell adhesion reveals a pathway leading to axonal degeneration. J Neurosci. 2009;29:118-30 pubmed publisher
Dosemeci A, Tao Cheng J, Bakly V, Reese T. Postsynaptic densities fragment into subcomplexes upon sonication. Mol Brain. 2019;12:72 pubmed publisher
Bragina L, Conti F. Expression of Neurofilament Subunits at Neocortical Glutamatergic and GABAergic Synapses. Front Neuroanat. 2018;12:74 pubmed publisher
Keshavarz M, Skill M, Hollenhorst M, Maxeiner S, Walecki M, Pfeil U, et al. Caveolin-3 differentially orchestrates cholinergic and serotonergic constriction of murine airways. Sci Rep. 2018;8:7508 pubmed publisher
Stoffel W, Jenke B, Schmidt Soltau I, Binczek E, Brodesser S, Hammels I. SMPD3 deficiency perturbs neuronal proteostasis and causes progressive cognitive impairment. Cell Death Dis. 2018;9:507 pubmed publisher
Yan L, Wei M, Gong A, Song P, Lou J, Bi C, et al. A Modified Chinese Herbal Decoction (Kai-Xin-San) Promotes NGF-Induced Neuronal Differentiation in PC12 Cells via Up-Regulating Trk A Signaling. Front Cell Dev Biol. 2017;5:118 pubmed publisher
Yoon H, Radulovic M, Walters G, Paulsen A, Drucker K, Starski P, et al. Protease activated receptor 2 controls myelin development, resiliency and repair. Glia. 2017;65:2070-2086 pubmed publisher
Du X, West M, Cai Q, Cheng W, EWERT D, Li W, et al. Antioxidants reduce neurodegeneration and accumulation of pathologic Tau proteins in the auditory system after blast exposure. Free Radic Biol Med. 2017;108:627-643 pubmed publisher
Lai F, Lau S, Wong J, Gui H, Wang R, Zhou T, et al. Correction of Hirschsprung-Associated Mutations in Human Induced Pluripotent Stem Cells Via Clustered Regularly Interspaced Short Palindromic Repeats/Cas9, Restores Neural Crest Cell Function. Gastroenterology. 2017;153:139-153.e8 pubmed publisher
Okamoto Uchida Y, Yu R, Miyamura N, Arima N, Ishigami Yuasa M, Kagechika H, et al. The mevalonate pathway regulates primitive streak formation via protein farnesylation. Sci Rep. 2016;6:37697 pubmed publisher
Shim M, Takihara Y, Kim K, Iwata T, Yue B, Inatani M, et al. Mitochondrial pathogenic mechanism and degradation in optineurin E50K mutation-mediated retinal ganglion cell degeneration. Sci Rep. 2016;6:33830 pubmed publisher
Maxeiner S, Luo F, Tan A, Schmitz F, Südhof T. How to make a synaptic ribbon: RIBEYE deletion abolishes ribbons in retinal synapses and disrupts neurotransmitter release. EMBO J. 2016;35:1098-114 pubmed publisher
Yoon H, Radulovic M, Drucker K, Wu J, Scarisbrick I. The thrombin receptor is a critical extracellular switch controlling myelination. Glia. 2015;63:846-59 pubmed publisher
Vermehren Schmaedick A, KRUEGER W, Jacob T, Ramunno Johnson D, Balkowiec A, Lidke K, et al. Heterogeneous intracellular trafficking dynamics of brain-derived neurotrophic factor complexes in the neuronal soma revealed by single quantum dot tracking. PLoS ONE. 2014;9:e95113 pubmed publisher
Liu C, Chien C. Molecular cloning and characterization of chicken neuronal intermediate filament protein ?-internexin. J Comp Neurol. 2013;521:2147-64 pubmed publisher
Sekigawa A, Fujita M, Sekiyama K, Takamatsu Y, Hatano T, Rockenstein E, et al. Distinct mechanisms of axonal globule formation in mice expressing human wild type ?-synuclein or dementia with Lewy bodies-linked P123H ?-synuclein. Mol Brain. 2012;5:34 pubmed publisher
Foller M, Jaumann M, Dettling J, Saxena A, Pakladok T, Munoz C, et al. AMP-activated protein kinase in BK-channel regulation and protection against hearing loss following acoustic overstimulation. FASEB J. 2012;26:4243-53 pubmed publisher
Louis E, Ma K, Babij R, Cortes E, LIEM R, Vonsattel J, et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett. 2012;518:49-54 pubmed publisher
Uchida A, Sasaguri H, Kimura N, Tajiri M, Ohkubo T, Ono F, et al. Non-human primate model of amyotrophic lateral sclerosis with cytoplasmic mislocalization of TDP-43. Brain. 2012;135:833-46 pubmed publisher
Barrientos S, Martinez N, Yoo S, Jara J, Zamorano S, Hetz C, et al. Axonal degeneration is mediated by the mitochondrial permeability transition pore. J Neurosci. 2011;31:966-78 pubmed publisher
Kovacs K, Lajtha A, Sershen H. Effect of nicotine and cocaine on neurofilaments and receptors in whole brain tissue and synaptoneurosome preparations. Brain Res Bull. 2010;82:109-17 pubmed publisher
Bocquet A, Berges R, Frank R, Robert P, Peterson A, Eyer J. Neurofilaments bind tubulin and modulate its polymerization. J Neurosci. 2009;29:11043-54 pubmed publisher
Iwatsuki H, Suda M. Transient expression of keratin during neuronal development in the adult rabbit spinal ganglion. Anat Sci Int. 2010;85:46-55 pubmed publisher
Heidrych P, Zimmermann U, Kuhn S, Franz C, Engel J, Duncker S, et al. Otoferlin interacts with myosin VI: implications for maintenance of the basolateral synaptic structure of the inner hair cell. Hum Mol Genet. 2009;18:2779-90 pubmed publisher
Balastik M, Ferraguti F, Pires da Silva A, Lee T, Alvarez Bolado G, Lu K, et al. Deficiency in ubiquitin ligase TRIM2 causes accumulation of neurofilament light chain and neurodegeneration. Proc Natl Acad Sci U S A. 2008;105:12016-21 pubmed publisher
Kindt F, Wiegand S, LOser C, Nilles M, Niemeier V, Hsu S, et al. Intermedin: a skin peptide that is downregulated in atopic dermatitis. J Invest Dermatol. 2007;127:605-13 pubmed
Williams K, Rahimtula M, Mearow K. Heat shock protein 27 is involved in neurite extension and branching of dorsal root ganglion neurons in vitro. J Neurosci Res. 2006;84:716-23 pubmed
García Fuster M, Miralles A, Garcia Sevilla J. Effects of opiate drugs on Fas-associated protein with death domain (FADD) and effector caspases in the rat brain: regulation by the ERK1/2 MAP kinase pathway. Neuropsychopharmacology. 2007;32:399-411 pubmed
Fasani F, Bocquet A, Robert P, Peterson A, Eyer J. The amount of neurofilaments aggregated in the cell body is controlled by their increased sensitivity to trypsin-like proteases. J Cell Sci. 2004;117:861-9 pubmed
Stiefel D, Shibata T, Meuli M, Duffy P, Copp A. Tethering of the spinal cord in mouse fetuses and neonates with spina bifida. J Neurosurg. 2003;99:206-13 pubmed
Robertson J, Beaulieu J, Doroudchi M, Durham H, Julien J, Mushynski W. Apoptotic death of neurons exhibiting peripherin aggregates is mediated by the proinflammatory cytokine tumor necrosis factor-alpha. J Cell Biol. 2001;155:217-26 pubmed
Ackerley S, Grierson A, Brownlees J, Thornhill P, Anderton B, Leigh P, et al. Glutamate slows axonal transport of neurofilaments in transfected neurons. J Cell Biol. 2000;150:165-76 pubmed
Canete Soler R, Silberg D, Gershon M, Schlaepfer W. Mutation in neurofilament transgene implicates RNA processing in the pathogenesis of neurodegenerative disease. J Neurosci. 1999;19:1273-83 pubmed
product information
Product No. :
N5139
Product Name :
Monoclonal Anti-Neurofilament 68 antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
NR4
Applications/Description :
Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using rat cerebellum; western blot: 1:500 using bovine spinal cord neurofilament; microarray
Species Reactivity :
rat, pig, chicken, human
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA