This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
protein
product name :
5′-Nucleotidase human
catalog :
N1665
citations: 115
Reference
Nigri J, Gironella M, Bressy C, Vila Navarro E, Roques J, Lac S, et al. PAP/REG3A favors perineural invasion in pancreatic adenocarcinoma and serves as a prognostic marker. Cell Mol Life Sci. 2017;74:4231-4243 pubmed publisher
Wang K, Wang K, Zhou N, Tian Y. Secretory Expression, Purification, Characterization, and Application of an Aspergillus oryzae Prolyl Aminopeptidase in Bacillus subtilis. Appl Biochem Biotechnol. 2017;181:1611-1623 pubmed publisher
Gao Z, Dong K, Zhang H. The roles of CD73 in cancer. Biomed Res Int. 2014;2014:460654 pubmed publisher
Lisignoli G, Manferdini C, Lambertini E, Zini N, Angelozzi M, Gabusi E, et al. Chondrogenic potential of Slug-depleted human mesenchymal stem cells. Tissue Eng Part A. 2014;20:2795-805 pubmed publisher
Ilyinskii P, Roy C, O Neil C, Browning E, Pittet L, Altreuter D, et al. Adjuvant-carrying synthetic vaccine particles augment the immune response to encapsulated antigen and exhibit strong local immune activation without inducing systemic cytokine release. Vaccine. 2014;32:2882-95 pubmed publisher
Coffey F, Lee S, Buus T, Lauritsen J, Wong G, Joachims M, et al. The TCR ligand-inducible expression of CD73 marks ?? lineage commitment and a metastable intermediate in effector specification. J Exp Med. 2014;211:329-43 pubmed publisher
Srinivasan B, Forouhar F, Shukla A, Sampangi C, Kulkarni S, Abashidze M, et al. Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila. FEBS J. 2014;281:1613-1628 pubmed publisher
Khatri A, Williams B, Fisher J, Brundage R, Gurvich V, Lis L, et al. SLC28A3 genotype and gemcitabine rate of infusion affect dFdCTP metabolite disposition in patients with solid tumours. Br J Cancer. 2014;110:304-12 pubmed publisher
Shah D, Romero F, Stafstrom W, Duong M, Summer R. Extracellular ATP mediates the late phase of neutrophil recruitment to the lung in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2014;306:L152-61 pubmed publisher
Tak E, Ridyard D, Kim J, Zimmerman M, Werner T, Wang X, et al. CD73-dependent generation of adenosine and endothelial Adora2b signaling attenuate diabetic nephropathy. J Am Soc Nephrol. 2014;25:547-63 pubmed publisher
Mason S, Tarle S, Osibin W, Kinfu Y, Kaigler D. Standardization and safety of alveolar bone-derived stem cell isolation. J Dent Res. 2014;93:55-61 pubmed publisher
Suprabha N, Pai C. Mesenchymal stem cells and granulomas in Crohn's disease and intestinal tuberculosis: the way forward. Dig Dis Sci. 2013;58:3641 pubmed publisher
Thompson L. Editorial: CD73 deficiency and immune dysregulation in HIV infection: cause or effect?. J Leukoc Biol. 2013;94:545-7 pubmed publisher
Machado P, Brady S, Hanna M. Update in inclusion body myositis. Curr Opin Rheumatol. 2013;25:763-71 pubmed publisher
Greenberg S. Biomarkers of inclusion body myositis. Curr Opin Rheumatol. 2013;25:753-62 pubmed publisher
Terp M, Olesen K, Arnspang E, Lund R, Lagerholm B, Ditzel H, et al. Anti-human CD73 monoclonal antibody inhibits metastasis formation in human breast cancer by inducing clustering and internalization of CD73 expressed on the surface of cancer cells. J Immunol. 2013;191:4165-73 pubmed publisher
Lee D, Taylor A. Both MC5r and A2Ar are required for protective regulatory immunity in the spleen of post-experimental autoimmune uveitis in mice. J Immunol. 2013;191:4103-11 pubmed publisher
Iyer S, Latner D, Zilliox M, McCausland M, Akondy R, Penaloza MacMaster P, et al. Identification of novel markers for mouse CD4(+) T follicular helper cells. Eur J Immunol. 2013;43:3219-32 pubmed publisher
Marsell R, Steen B, Bais M, Mortlock D, Einhorn T, Gerstenfeld L. Skeletal trauma generates systemic BMP2 activation that is temporally related to the mobilization of CD73+ cells. J Orthop Res. 2014;32:17-23 pubmed publisher
Furtmann N, Bajorath J. Evaluation of molecular model-based discovery of ecto-5'-nucleotidase inhibitors on the basis of X-ray structures. Bioorg Med Chem. 2013;21:6616-22 pubmed publisher
Allard B, Pommey S, Smyth M, Stagg J. Targeting CD73 enhances the antitumor activity of anti-PD-1 and anti-CTLA-4 mAbs. Clin Cancer Res. 2013;19:5626-35 pubmed publisher
Allard B, Turcotte M, Spring K, Pommey S, Royal I, Stagg J. Anti-CD73 therapy impairs tumor angiogenesis. Int J Cancer. 2014;134:1466-73 pubmed publisher
Beavis P, Divisekera U, Paget C, Chow M, John L, Devaud C, et al. Blockade of A2A receptors potently suppresses the metastasis of CD73+ tumors. Proc Natl Acad Sci U S A. 2013;110:14711-6 pubmed publisher
Tai N, Wong F, Wen L. TLR9 deficiency promotes CD73 expression in T cells and diabetes protection in nonobese diabetic mice. J Immunol. 2013;191:2926-37 pubmed publisher
Govindaraj C, Scalzo Inguanti K, Madondo M, Hallo J, Flanagan K, Quinn M, et al. Impaired Th1 immunity in ovarian cancer patients is mediated by TNFR2+ Tregs within the tumor microenvironment. Clin Immunol. 2013;149:97-110 pubmed publisher
Pettengill M, Robson S, Tresenriter M, Millan J, Usheva A, Bingham T, et al. Soluble ecto-5'-nucleotidase (5'-NT), alkaline phosphatase, and adenosine deaminase (ADA1) activities in neonatal blood favor elevated extracellular adenosine. J Biol Chem. 2013;288:27315-26 pubmed publisher
Al Jafar H, Layton D, Robertson L, Escuredo E, Bain B. Diagnosis of pyrimidine 5'-nucleotidase deficiency suspected from a blood film. Am J Hematol. 2013;88:1089 pubmed publisher
Pluskota E, Ma Y, Bledzka K, Bialkowska K, Soloviev D, Szpak D, et al. Kindlin-2 regulates hemostasis by controlling endothelial cell-surface expression of ADP/AMP catabolic enzymes via a clathrin-dependent mechanism. Blood. 2013;122:2491-9 pubmed publisher
Grozio A, Sociali G, Sturla L, Caffa I, Soncini D, Salis A, et al. CD73 protein as a source of extracellular precursors for sustained NAD+ biosynthesis in FK866-treated tumor cells. J Biol Chem. 2013;288:25938-49 pubmed publisher
Larman H, Salajegheh M, Nazareno R, Lam T, Sauld J, Steen H, et al. Cytosolic 5'-nucleotidase 1A autoimmunity in sporadic inclusion body myositis. Ann Neurol. 2013;73:408-18 pubmed publisher
Zhang W, Zhang Y, Wang W, Dai Y, Ning C, Luo R, et al. Elevated ecto-5'-nucleotidase-mediated increased renal adenosine signaling via A2B adenosine receptor contributes to chronic hypertension. Circ Res. 2013;112:1466-78 pubmed publisher
Wang L, Tang S, Wang Y, Xu S, Yu J, Zhi X, et al. Ecto-5'-nucleotidase (CD73) promotes tumor angiogenesis. Clin Exp Metastasis. 2013;30:671-80 pubmed publisher
Shechter R, Miller O, Yovel G, Rosenzweig N, London A, Ruckh J, et al. Recruitment of beneficial M2 macrophages to injured spinal cord is orchestrated by remote brain choroid plexus. Immunity. 2013;38:555-69 pubmed publisher
Aster J, DeAngelo D. Resistance revealed in acute lymphoblastic leukemia. Nat Med. 2013;19:264-5 pubmed publisher
Pluk H, van Hoeve B, van Dooren S, Stammen Vogelzangs J, van der Heijden A, Schelhaas H, et al. Autoantibodies to cytosolic 5'-nucleotidase 1A in inclusion body myositis. Ann Neurol. 2013;73:397-407 pubmed publisher
Rust S, Guillard S, Sachsenmeier K, Hay C, Davidson M, Karlsson A, et al. Combining phenotypic and proteomic approaches to identify membrane targets in a 'triple negative' breast cancer cell type. Mol Cancer. 2013;12:11 pubmed publisher
Chen M, Su W, Lin X, Guo Z, Wang J, Zhang Q, et al. Adoptive transfer of human gingiva-derived mesenchymal stem cells ameliorates collagen-induced arthritis via suppression of Th1 and Th17 cells and enhancement of regulatory T cell differentiation. Arthritis Rheum. 2013;65:1181-93 pubmed publisher
Tzoneva G, Perez Garcia A, Carpenter Z, Khiabanian H, Tosello V, Allegretta M, et al. Activating mutations in the NT5C2 nucleotidase gene drive chemotherapy resistance in relapsed ALL. Nat Med. 2013;19:368-71 pubmed publisher
Meyer J, Wang J, Hogan L, Yang J, Dandekar S, Patel J, et al. Relapse-specific mutations in NT5C2 in childhood acute lymphoblastic leukemia. Nat Genet. 2013;45:290-4 pubmed publisher
Sweet R, Cullen J, Shlomchik M. Rheumatoid factor B cell memory leads to rapid, switched antibody-forming cell responses. J Immunol. 2013;190:1974-81 pubmed publisher
Krug U, Patzschke R, Zebisch M, Balbach J, Strater N. Contribution of the two domains of E. coli 5'-nucleotidase to substrate specificity and catalysis. FEBS Lett. 2013;587:460-6 pubmed publisher
Rylova J, Andreeva E, Gogvadze V, Zhivotovsky B, Buravkova L. Etoposide and hypoxia do not activate apoptosis of multipotent mesenchymal stromal cells in vitro. Bull Exp Biol Med. 2012;154:141-4 pubmed
Khalpey Z, Yacoub M, Smolenski R. Nucleotide metabolic mismatches in mammalian hearts: implications for transplantation. Ann R Coll Surg Engl. 2013;95:9-14 pubmed publisher
Ode A, Schoon J, Kurtz A, Gaetjen M, Ode J, Geissler S, et al. CD73/5'-ecto-nucleotidase acts as a regulatory factor in osteo-/chondrogenic differentiation of mechanically stimulated mesenchymal stromal cells. Eur Cell Mater. 2013;25:37-47 pubmed
Giarola N, de Almeida Amaral E, Collopy Júnior I, Fonseca de Souza A, Majerowicz D, Paes L, et al. Trypanosoma cruzi: effects of heat shock on ecto-ATPase activity. Exp Parasitol. 2013;133:434-41 pubmed publisher
Ohta M, Toyama K, Gutterman D, Campbell W, Lemaitre V, Teraoka R, et al. Ecto-5'-nucleotidase, CD73, is an endothelium-derived hyperpolarizing factor synthase. Arterioscler Thromb Vasc Biol. 2013;33:629-36 pubmed publisher
Ali Z, Bakli M, Fontaine A, Bakkali N, Vu Hai V, Audebert S, et al. Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites. Malar J. 2012;11:439 pubmed publisher
Mohsen Kanson T, Hafner A, Wdziekonski B, Villageois P, Chignon Sicard B, Dani C. Expression of cell surface markers during self-renewal and differentiation of human adipose-derived stem cells. Biochem Biophys Res Commun. 2013;430:871-5 pubmed publisher
Rajasekaran N, Wang N, Truong P, Rinderknecht C, Macaubas C, Beilhack G, et al. Host-derived CD4+ T cells attenuate stem cell-mediated transfer of autoimmune arthritis in lethally irradiated C57BL/6.g7 mice. Arthritis Rheum. 2013;65:681-92 pubmed publisher
Buschmann J, Moritz B, Jeske M, Lilie H, Schierhorn A, Wahle E. Identification of Drosophila and human 7-methyl GMP-specific nucleotidases. J Biol Chem. 2013;288:2441-51 pubmed publisher
Jordheim L, Marton Z, Rhimi M, Cros Perrial E, Lionne C, Peyrottes S, et al. Identification and characterization of inhibitors of cytoplasmic 5'-nucleotidase cN-II issued from virtual screening. Biochem Pharmacol. 2013;85:497-506 pubmed publisher
Regateiro F, Cobbold S, Waldmann H. CD73 and adenosine generation in the creation of regulatory microenvironments. Clin Exp Immunol. 2013;171:1-7 pubmed publisher
Knapp K, Zebisch M, Strater N. Crystallization and preliminary X-ray analysis of the open form of human ecto-5'-nucleotidase (CD73). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:1545-9 pubmed publisher
Sadej R, Skladanowski A. Dual, enzymatic and non-enzymatic, function of ecto-5'-nucleotidase (eN, CD73) in migration and invasion of A375 melanoma cells. Acta Biochim Pol. 2012;59:647-52 pubmed
Patkar C, Giaya K, Libraty D. Dengue virus type 2 modulates endothelial barrier function through CD73. Am J Trop Med Hyg. 2013;88:89-94 pubmed publisher
Knapp K, Zebisch M, Pippel J, El Tayeb A, Muller C, Strater N. Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling. Structure. 2012;20:2161-73 pubmed publisher
Garavaglia S, Bruzzone S, Cassani C, Canella L, Allegrone G, Sturla L, et al. The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism. Biochem J. 2012;441:131-41 pubmed publisher
Graddis T, McMahan C, Tamman J, Page K, Trager J. Prostatic acid phosphatase expression in human tissues. Int J Clin Exp Pathol. 2011;4:295-306 pubmed
St Hilaire C, Ziegler S, Markello T, Brusco A, Groden C, Gill F, et al. NT5E mutations and arterial calcifications. N Engl J Med. 2011;364:432-42 pubmed publisher
Burkard T, Planyavsky M, Kaupe I, Breitwieser F, Burckstummer T, Bennett K, et al. Initial characterization of the human central proteome. BMC Syst Biol. 2011;5:17 pubmed publisher
Chuang T, Chen S, Lin F, Veeramani S, Kumar S, Batra S, et al. Human prostatic acid phosphatase, an authentic tyrosine phosphatase, dephosphorylates ErbB-2 and regulates prostate cancer cell growth. J Biol Chem. 2010;285:23598-606 pubmed publisher
Nanga R, Brender J, Vivekanandan S, Popovych N, Ramamoorthy A. NMR structure in a membrane environment reveals putative amyloidogenic regions of the SEVI precursor peptide PAP(248-286). J Am Chem Soc. 2009;131:17972-9 pubmed publisher
Roan N, Sowinski S, Munch J, Kirchhoff F, Greene W. Aminoquinoline surfen inhibits the action of SEVI (semen-derived enhancer of viral infection). J Biol Chem. 2010;285:1861-9 pubmed publisher
Choudhary C, Kumar C, Gnad F, Nielsen M, Rehman M, Walther T, et al. Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science. 2009;325:834-40 pubmed publisher
Hauber I, Hohenberg H, Holstermann B, Hunstein W, Hauber J. The main green tea polyphenol epigallocatechin-3-gallate counteracts semen-mediated enhancement of HIV infection. Proc Natl Acad Sci U S A. 2009;106:9033-8 pubmed publisher
Hong S, Klein E, Das Gupta J, Hanke K, Weight C, Nguyen C, et al. Fibrils of prostatic acid phosphatase fragments boost infections with XMRV (xenotropic murine leukemia virus-related virus), a human retrovirus associated with prostate cancer. J Virol. 2009;83:6995-7003 pubmed publisher
Wollscheid B, Bausch Fluck D, Henderson C, O Brien R, Bibel M, Schiess R, et al. Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins. Nat Biotechnol. 2009;27:378-86 pubmed publisher
Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, et al. Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res. 2009;8:651-61 pubmed publisher
Dephoure N, Zhou C, Villen J, Beausoleil S, Bakalarski C, Elledge S, et al. A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008;105:10762-7 pubmed publisher
Munch J, Rücker E, Ständker L, Adermann K, Goffinet C, Schindler M, et al. Semen-derived amyloid fibrils drastically enhance HIV infection. Cell. 2007;131:1059-71 pubmed
Schröder B, Wrocklage C, Pan C, Jäger R, Kösters B, Schafer H, et al. Integral and associated lysosomal membrane proteins. Traffic. 2007;8:1676-86 pubmed
Quintero I, Araujo C, Pulkka A, Wirkkala R, Herrala A, Eskelinen E, et al. Prostatic acid phosphatase is not a prostate specific target. Cancer Res. 2007;67:6549-54 pubmed
Wallden K, Stenmark P, Nyman T, Flodin S, Gräslund S, Loppnau P, et al. Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition. J Biol Chem. 2007;282:17828-36 pubmed
Gulland J, Jackson E. 5-Nucleotidase. Biochem J. 1938;32:597-601 pubmed
Gregory S, Barlow K, McLay K, Kaul R, Swarbreck D, Dunham A, et al. The DNA sequence and biological annotation of human chromosome 1. Nature. 2006;441:315-21 pubmed
Zody M, Garber M, Adams D, Sharpe T, Harrow J, Lupski J, et al. DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage. Nature. 2006;440:1045-9 pubmed
Otsuki T, Ota T, Nishikawa T, Hayashi K, Suzuki Y, Yamamoto J, et al. Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res. 2005;12:117-26 pubmed
Amici A, Ciccioli K, Naponelli V, Raffaelli N, Magni G. Evidence for essential catalytic determinants for human erythrocyte pyrimidine 5'-nucleotidase. Cell Mol Life Sci. 2005;62:1613-20 pubmed
Hillier L, Graves T, Fulton R, Fulton L, Pepin K, Minx P, et al. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. Nature. 2005;434:724-31 pubmed
Gerhard D, Wagner L, Feingold E, Shenmen C, Grouse L, Schuler G, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14:2121-7 pubmed
Tanaka M, Kishi Y, Takanezawa Y, Kakehi Y, Aoki J, Arai H. Prostatic acid phosphatase degrades lysophosphatidic acid in seminal plasma. FEBS Lett. 2004;571:197-204 pubmed
Kanno H, Takizawa T, Miwa S, Fujii H. Molecular basis of Japanese variants of pyrimidine 5'-nucleotidase deficiency. Br J Haematol. 2004;126:265-71 pubmed
Deloukas P, Earthrowl M, Grafham D, Rubenfield M, French L, Steward C, et al. The DNA sequence and comparative analysis of human chromosome 10. Nature. 2004;429:375-81 pubmed
Heppel L, HILMORE R. Purification and properties of 5-nucleotidase. J Biol Chem. 1951;188:665-76 pubmed
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004;36:40-5 pubmed
Mungall A, Palmer S, Sims S, Edwards C, Ashurst J, Wilming L, et al. The DNA sequence and analysis of human chromosome 6. Nature. 2003;425:805-11 pubmed
Segal H, Brenner B. 5'-Nucleotidase of rat liver microsomes. J Biol Chem. 1960;235:471-4 pubmed
Bianchi P, Fermo E, Alfinito F, Vercellati C, Baserga M, Ferraro F, et al. Molecular characterization of six unrelated Italian patients affected by pyrimidine 5'-nucleotidase deficiency. Br J Haematol. 2003;122:847-51 pubmed
Hillier L, Fulton R, Fulton L, Graves T, Pepin K, Wagner McPherson C, et al. The DNA sequence of human chromosome 7. Nature. 2003;424:157-64 pubmed
Ortlund E, LaCount M, Lebioda L. Crystal structures of human prostatic acid phosphatase in complex with a phosphate ion and alpha-benzylaminobenzylphosphonic acid update the mechanistic picture and offer new insights into inhibitor design. Biochemistry. 2003;42:383-9 pubmed
Sala Newby G, Newby A. Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein. Biochim Biophys Acta. 2001;1521:12-8 pubmed
Marinaki A, Escuredo E, Duley J, Simmonds H, Amici A, Naponelli V, et al. Genetic basis of hemolytic anemia caused by pyrimidine 5' nucleotidase deficiency. Blood. 2001;97:3327-32 pubmed
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Amici A, Emanuelli M, Raffaelli N, Ruggieri S, Saccucci F, Magni G. Human erythrocyte pyrimidine 5-nucleotidase, PN-I, is identical to p36, a protein associated to lupus inclusion formation in response to alpha-interferon. Blood. 2000;96:1596-8 pubmed
Jakob C, Lewinski K, Kuciel R, Ostrowski W, Lebioda L. Crystal structure of human prostatic acid phosphatase . Prostate. 2000;42:211-8 pubmed
LaCount M, Handy G, Lebioda L. Structural origins of L(+)-tartrate inhibition of human prostatic acid phosphatase. J Biol Chem. 1998;273:30406-9 pubmed
Zhang Z, Ostanin K, Van Etten R. Covalent modification and site-directed mutagenesis of an active site tryptophan of human prostatic acid phosphatase. Acta Biochim Pol. 1997;44:659-72 pubmed
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product information
Catalog Number :
N1665
Product Name :
5′-Nucleotidase human
Product Type :
PROTEINS & ENZYMES
Product Group :
Protein & Pathway Technologies
Product Description :
recombinant, expressed in CHO cells, vial of 6-12 ?g
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA