product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Myosin (Smooth) antibody produced in mouse
catalog :
M7786
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
hSM-V
reactivity :
human, mouse, rat, dogs, chicken, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 61
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; loading ...; fig 5a
Domke L, Franke W. The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system. Cell Tissue Res. 2019;375:451-482 pubmed publisher
  • western blot; mouse; fig 7
Llorian M, Gooding C, Bellora N, Hallegger M, Buckroyd A, Wang X, et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res. 2016;44:8933-8950 pubmed
  • western blot; human; 1:1000; fig 2
Chen P, Qin L, Li G, Tellides G, Simons M. Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression. EMBO Mol Med. 2016;8:712-28 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig s4e
Fattahi F, Steinbeck J, Kriks S, Tchieu J, Zimmer B, Kishinevsky S, et al. Deriving human ENS lineages for cell therapy and drug discovery in Hirschsprung disease. Nature. 2016;531:105-9 pubmed publisher
  • immunocytochemistry; human
Su K, Edwards S, Tan K, White J, Kandel S, Ramshaw J, et al. Induction of endometrial mesenchymal stem cells into tissue-forming cells suitable for fascial repair. Acta Biomater. 2014;10:5012-5020 pubmed publisher
  • immunocytochemistry; bovine
  • western blot; bovine
  • flow cytometry; mouse
  • immunocytochemistry; mouse
  • western blot; mouse
  • immunocytochemistry; rat
  • western blot; rat
Kennedy E, Mooney C, Hakimjavadi R, Fitzpatrick E, Guha S, Collins L, et al. Adult vascular smooth muscle cells in culture express neural stem cell markers typical of resident multipotent vascular stem cells. Cell Tissue Res. 2014;358:203-16 pubmed publisher
  • flow cytometry; rat
  • immunocytochemistry; rat
  • western blot; rat
Kennedy E, Hakimjavadi R, Greene C, Mooney C, Fitzpatrick E, Collins L, et al. Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells?. Vasc Cell. 2014;6:6 pubmed publisher
Norton E, Bridges L, Kenyon L, Esiri M, Bennett D, Hainsworth A. Cell Senescence and Cerebral Small Vessel Disease in the Brains of People Aged 80 Years and Older. J Neuropathol Exp Neurol. 2019;78:1066-1072 pubmed publisher
Ventura Ferreira M, Bienert M, Muller K, Rath B, Goecke T, Opländer C, et al. Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta. Stem Cell Res Ther. 2018;9:28 pubmed publisher
Liskova J, Hadraba D, Filova E, Konarik M, Pirk J, Jelen K, et al. Valve interstitial cell culture: Production of mature type I collagen and precise detection. Microsc Res Tech. 2017;80:936-942 pubmed publisher
Belle M, Godefroy D, Couly G, Malone S, Collier F, Giacobini P, et al. Tridimensional Visualization and Analysis of Early Human Development. Cell. 2017;169:161-173.e12 pubmed publisher
Li G, Qin L, Wang L, Li X, Caulk A, Zhang J, et al. Inhibition of the mTOR pathway in abdominal aortic aneurysm: implications of smooth muscle cell contractile phenotype, inflammation, and aneurysm expansion. Am J Physiol Heart Circ Physiol. 2017;312:H1110-H1119 pubmed publisher
Chen P, Qin L, Li G, Tellides G, Simons M. Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGF?)-dependent smooth muscle cell phenotype modulation. Sci Rep. 2016;6:33407 pubmed publisher
Boehme J, Sun X, Tormos K, Gong W, Kellner M, Datar S, et al. Pulmonary artery smooth muscle cell hyperproliferation and metabolic shift triggered by pulmonary overcirculation. Am J Physiol Heart Circ Physiol. 2016;311:H944-H957 pubmed publisher
Chen P, Qin L, Baeyens N, Li G, Afolabi T, Budatha M, et al. Endothelial-to-mesenchymal transition drives atherosclerosis progression. J Clin Invest. 2015;125:4514-28 pubmed publisher
Xu Z, Zhang Q, Li H. Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor. Mol Med Rep. 2015;12:6005-12 pubmed publisher
Thomas A, Eijgelaar W, Daemen M, Newby A. Foam Cell Formation In Vivo Converts Macrophages to a Pro-Fibrotic Phenotype. PLoS ONE. 2015;10:e0128163 pubmed publisher
Huttala O, Vuorenpää H, Toimela T, Uotila J, Kuokkanen H, Ylikomi T, et al. Human vascular model with defined stimulation medium - a characterization study. ALTEX. 2015;32:125-36 pubmed publisher
Hashemi Gheinani A, Burkhard F, Rehrauer H, Aquino Fournier C, Monastyrskaya K. MicroRNA MiR-199a-5p regulates smooth muscle cell proliferation and morphology by targeting WNT2 signaling pathway. J Biol Chem. 2015;290:7067-86 pubmed publisher
Chen C, Tao T, Wen C, He W, Qiao Y, Gao Y, et al. Myosin light chain kinase (MLCK) regulates cell migration in a myosin regulatory light chain phosphorylation-independent mechanism. J Biol Chem. 2014;289:28478-88 pubmed publisher
Stamatiou R, Paraskeva E, Vasilaki A, Mylonis I, Molyvdas P, Gourgoulianis K, et al. Long-term exposure to muscarinic agonists decreases expression of contractile proteins and responsiveness of rabbit tracheal smooth muscle cells. BMC Pulm Med. 2014;14:39 pubmed publisher
Aggarwal S, Gross C, Rafikov R, Kumar S, Fineman J, Ludewig B, et al. Nitration of tyrosine 247 inhibits protein kinase G-1? activity by attenuating cyclic guanosine monophosphate binding. J Biol Chem. 2014;289:7948-61 pubmed publisher
Lakhani H, de Mel A, Seifalian A. The effect of TGF-β1 and BMP-4 on bone marrow-derived stem cell morphology on a novel bioabsorbable nanocomposite material. Artif Cells Nanomed Biotechnol. 2015;43:230-4 pubmed publisher
Matsubayashi M, Teramoto Kimata I, Uni S, Lillehoj H, Matsuda H, Furuya M, et al. Elongation factor-1? is a novel protein associated with host cell invasion and a potential protective antigen of Cryptosporidium parvum. J Biol Chem. 2013;288:34111-20 pubmed publisher
Xu Z, Zhang Q, Li H. Human hair follicle stem cell differentiation into contractile smooth muscle cells is induced by transforming growth factor-?1 and platelet-derived growth factor BB. Mol Med Rep. 2013;8:1715-21 pubmed publisher
Sundaresan N, Vasudevan P, Zhong L, Kim G, Samant S, Parekh V, et al. The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy by targeting c-Jun. Nat Med. 2012;18:1643-50 pubmed publisher
Wang C, Guo F, Zhou H, Zhang Y, Xiao Z, Cui L. Proteomic profiling of tissue-engineered blood vessel walls constructed by adipose-derived stem cells. Tissue Eng Part A. 2013;19:415-25 pubmed publisher
Raphel L, Talasila A, Cheung C, Sinha S. Myocardin overexpression is sufficient for promoting the development of a mature smooth muscle cell-like phenotype from human embryonic stem cells. PLoS ONE. 2012;7:e44052 pubmed publisher
Strauss M, Rada A, Tejero F, Hermoso T. Heat stress in rat adriamycin cardiomyopathy: heat shock protein 25 and Myosin accumulation. J Toxicol Pathol. 2010;23:235-43 pubmed publisher
Xiao Q, Pepe A, Wang G, Luo Z, Zhang L, Zeng L, et al. Nrf3-Pla2g7 interaction plays an essential role in smooth muscle differentiation from stem cells. Arterioscler Thromb Vasc Biol. 2012;32:730-44 pubmed publisher
Datar S, Johnson E, Oishi P, Johengen M, Tang E, Aramburo A, et al. Altered lymphatics in an ovine model of congenital heart disease with increased pulmonary blood flow. Am J Physiol Lung Cell Mol Physiol. 2012;302:L530-40 pubmed publisher
Boopathi E, Gomes C, Goldfarb R, John M, Srinivasan V, Alanzi J, et al. Transcriptional repression of Caveolin-1 (CAV1) gene expression by GATA-6 in bladder smooth muscle hypertrophy in mice and human beings. Am J Pathol. 2011;178:2236-51 pubmed publisher
Boyd N, Nunes S, Jokinen J, Krishnan L, Chen Y, Smith K, et al. Microvascular mural cell functionality of human embryonic stem cell-derived mesenchymal cells. Tissue Eng Part A. 2011;17:1537-48 pubmed publisher
Bentley J, Popova A, Bozyk P, Linn M, Baek A, Lei J, et al. Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype. Respir Res. 2010;11:127 pubmed publisher
Schell C, Albrecht M, Spillner S, Mayer C, Kunz L, Kohn F, et al. 15-Deoxy-delta 12-14-prostaglandin-J2 induces hypertrophy and loss of contractility in human testicular peritubular cells: implications for human male fertility. Endocrinology. 2010;151:1257-68 pubmed publisher
Parizek M, Kasalkova N, Bacakova L, Slepicka P, Lisa V, Blazkova M, et al. Improved adhesion, growth and maturation of vascular smooth muscle cells on polyethylene grafted with bioactive molecules and carbon particles. Int J Mol Sci. 2009;10:4352-74 pubmed publisher
Wang C, Yin S, Cen L, Liu Q, Liu W, Cao Y, et al. Differentiation of adipose-derived stem cells into contractile smooth muscle cells induced by transforming growth factor-beta1 and bone morphogenetic protein-4. Tissue Eng Part A. 2010;16:1201-13 pubmed publisher
Ustiyan V, Wang I, Ren X, Zhang Y, Snyder J, Xu Y, et al. Forkhead box M1 transcriptional factor is required for smooth muscle cells during embryonic development of blood vessels and esophagus. Dev Biol. 2009;336:266-79 pubmed publisher
Wang C, Cen L, Yin S, Liu Q, Liu W, Cao Y, et al. A small diameter elastic blood vessel wall prepared under pulsatile conditions from polyglycolic acid mesh and smooth muscle cells differentiated from adipose-derived stem cells. Biomaterials. 2010;31:621-30 pubmed publisher
Yuen S, Ogut O, Brozovich F. Nonmuscle myosin is regulated during smooth muscle contraction. Am J Physiol Heart Circ Physiol. 2009;297:H191-9 pubmed publisher
Tréguer K, Naye F, Thiebaud P, Fedou S, Soulet F, Theze N, et al. Smooth muscle cell differentiation from human bone marrow: variations in cell type specific markers and Id gene expression in a new model of cell culture. Cell Biol Int. 2009;33:621-31 pubmed publisher
Margariti A, Xiao Q, Zampetaki A, Zhang Z, Li H, Martin D, et al. Splicing of HDAC7 modulates the SRF-myocardin complex during stem-cell differentiation towards smooth muscle cells. J Cell Sci. 2009;122:460-70 pubmed publisher
Bentley J, Deng H, Linn M, Lei J, Dokshin G, Fingar D, et al. Airway smooth muscle hyperplasia and hypertrophy correlate with glycogen synthase kinase-3(beta) phosphorylation in a mouse model of asthma. Am J Physiol Lung Cell Mol Physiol. 2009;296:L176-84 pubmed publisher
Schwab K, Gargett C. Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium. Hum Reprod. 2007;22:2903-11 pubmed
Pucovský V, Harhun M, Povstyan O, Gordienko D, Moss R, Bolton T. Close relation of arterial ICC-like cells to the contractile phenotype of vascular smooth muscle cell. J Cell Mol Med. 2007;11:764-75 pubmed
Grauss R, Winter E, van Tuyn J, Pijnappels D, Steijn R, Hogers B, et al. Mesenchymal stem cells from ischemic heart disease patients improve left ventricular function after acute myocardial infarction. Am J Physiol Heart Circ Physiol. 2007;293:H2438-47 pubmed
Ross J, Hong Z, Willenbring B, Zeng L, Isenberg B, Lee E, et al. Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells. J Clin Invest. 2006;116:3139-49 pubmed
Waltregny D, Glenisson W, Tran S, North B, Verdin E, Colige A, et al. Histone deacetylase HDAC8 associates with smooth muscle alpha-actin and is essential for smooth muscle cell contractility. FASEB J. 2005;19:966-8 pubmed
Walles T, Giere B, Hofmann M, Schanz J, Hofmann F, Mertsching H, et al. Experimental generation of a tissue-engineered functional and vascularized trachea. J Thorac Cardiovasc Surg. 2004;128:900-6 pubmed
Stolle K, Weitkamp B, Rauterberg J, Lorkowski S, Cullen P. Laser microdissection-based analysis of mRNA expression in human coronary arteries with intimal thickening. J Histochem Cytochem. 2004;52:1511-8 pubmed
Gajavelli S, Wood P, Pennica D, Whittemore S, Tsoulfas P. BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells. Exp Neurol. 2004;188:205-23 pubmed
Mann K, Ray J, Moon E, Sass K, Benson M. Calcineurin initiates smooth muscle differentiation in neural crest stem cells. J Cell Biol. 2004;165:483-91 pubmed
Kattan J, Dettman R, Bristow J. Formation and remodeling of the coronary vascular bed in the embryonic avian heart. Dev Dyn. 2004;230:34-43 pubmed
Halayko A, Kartha S, Stelmack G, McConville J, Tam J, Camoretti Mercado B, et al. Phophatidylinositol-3 kinase/mammalian target of rapamycin/p70S6K regulates contractile protein accumulation in airway myocyte differentiation. Am J Respir Cell Mol Biol. 2004;31:266-75 pubmed
Chambers R, Leoni P, Kaminski N, Laurent G, Heller R. Global expression profiling of fibroblast responses to transforming growth factor-beta1 reveals the induction of inhibitor of differentiation-1 and provides evidence of smooth muscle cell phenotypic switching. Am J Pathol. 2003;162:533-46 pubmed
Dodson A, Rhoden K. Bradykinin increases Na(+)-K(+) pump activity in cultured guinea-pig tracheal smooth muscle cells. Br J Pharmacol. 2001;133:1339-45 pubmed
Hypolite J, DiSanto M, Zheng Y, Chang S, Wein A, Chacko S. Regional variation in myosin isoforms and phosphorylation at the resting tone in urinary bladder smooth muscle. Am J Physiol Cell Physiol. 2001;280:C254-64 pubmed
Jenson H, Montalvo E, McClain K, Ench Y, Heard P, Christy B, et al. Characterization of natural Epstein-Barr virus infection and replication in smooth muscle cells from a leiomyosarcoma. J Med Virol. 1999;57:36-46 pubmed
Jobson T, Billington C, Hall I. Regulation of proliferation of human colonic subepithelial myofibroblasts by mediators important in intestinal inflammation. J Clin Invest. 1998;101:2650-7 pubmed
Burgess T, Fisher E, Ross S, Bready J, Qian Y, Bayewitch L, et al. The antiproliferative activity of c-myb and c-myc antisense oligonucleotides in smooth muscle cells is caused by a nonantisense mechanism. Proc Natl Acad Sci U S A. 1995;92:4051-5 pubmed
Frid M, Shekhonin B, Koteliansky V, Glukhova M. Phenotypic changes of human smooth muscle cells during development: late expression of heavy caldesmon and calponin. Dev Biol. 1992;153:185-93 pubmed
product information
Product No. :
M7786
Product Name :
Monoclonal Anti-Myosin (Smooth) antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
hSM-V
Applications/Description :
Suitable for immunohistochemistry (frozen sections) using human and animal tissue; western blot; immunoprecipitation (IP); immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human and animal tissue
Species Reactivity :
canine, pig, guinea pig, rabbit, human, chicken
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA