product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit
catalog :
M5670
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot
more info or order :
citations: 76
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 6i
Haag A, Walser M, Henggeler A, Hajnal A. The CHORD protein CHP-1 regulates EGF receptor trafficking and signaling in C. elegans and in human cells. elife. 2020;9: pubmed publisher
  • western blot; human; 1:10,000; loading ...; fig s1b
Kennedy S, Jarboui M, Srihari S, Raso C, Bryan K, Dernayka L, et al. Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRASG13D. Nat Commun. 2020;11:499 pubmed publisher
  • western blot; human; 1:2000; fig 4b
Olivares O, Mayers J, Gouirand V, Torrence M, Gicquel T, Borge L, et al. Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions. Nat Commun. 2017;8:16031 pubmed publisher
  • western blot; human; loading ...; tbl 4
Lopes M, Leal R, Guarnieri R, Schwarzbold M, Hoeller A, Diaz A, et al. A single high dose of dexamethasone affects the phosphorylation state of glutamate AMPA receptors in the human limbic system. Transl Psychiatry. 2016;6:e986 pubmed publisher
  • western blot; mouse; 1:2000; fig 5
Yang H, Vainshtein A, Maik Rachline G, Peles E. G protein-coupled receptor 37 is a negative regulator of oligodendrocyte differentiation and myelination. Nat Commun. 2016;7:10884 pubmed publisher
  • western blot; human; loading ...; fig 3b
Bigenzahn J, Fauster A, Rebsamen M, Kandasamy R, Scorzoni S, Vladimer G, et al. An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client. Mol Cell Proteomics. 2016;15:1139-50 pubmed
  • western blot; human; 1:10,000; fig 5
Stefanovic M, Tutusaus A, Martinez Nieto G, Bárcena C, de Gregorio E, Moutinho C, et al. Targeting glucosylceramide synthase upregulation reverts sorafenib resistance in experimental hepatocellular carcinoma. Oncotarget. 2016;7:8253-67 pubmed publisher
  • western blot; rat; 1:40,000; loading ...; fig s2a
Peres T, Ong L, Costa A, Eyng H, Venske D, Colle D, et al. Tyrosine hydroxylase regulation in adult rat striatum following short-term neonatal exposure to manganese. Metallomics. 2016;8:597-604 pubmed publisher
Inamdar V, Reddy H, Dangelmaier C, Kostyak J, Kunapuli S. The protein tyrosine phosphatase PTPN7 is a negative regulator of ERK activation and thromboxane generation in platelets. J Biol Chem. 2019;294:12547-12554 pubmed publisher
Sumi N, Ctortecka C, Hu Q, Bryant A, Fang B, Remsing Rix L, et al. Divergent Polypharmacology-Driven Cellular Activity of Structurally Similar Multi-Kinase Inhibitors through Cumulative Effects on Individual Targets. Cell Chem Biol. 2019;26:1240-1252.e11 pubmed publisher
Zingkou E, Pampalakis G, Charla E, Nauroy P, Kiritsi D, Sotiropoulou G. A proinflammatory role of KLK6 protease in Netherton syndrome. J Dermatol Sci. 2019;95:28-35 pubmed publisher
Thotakura S, Basova L, Makarenkova H. FGF Gradient Controls Boundary Position Between Proliferating and Differentiating Cells and Regulates Lacrimal Gland Growth Dynamics. Front Genet. 2019;10:362 pubmed publisher
Liu S, Xing Y, Wang J, Pan R, Li G, Tang H, et al. The Dual Role of HIV-1 gp120 V3 Loop-Induced Autophagy in the Survival and Apoptosis of the Primary Rat Hippocampal Neurons. Neurochem Res. 2019;44:1636-1652 pubmed publisher
Ning S, Wang Z, Cao J, Dong Y, Chen Y. Mel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling Pathway. Int J Mol Sci. 2019;20: pubmed publisher
Castany S, Codony X, Zamanillo D, Merlos M, Verdú E, Boadas Vaello P. Repeated Sigma-1 Receptor Antagonist MR309 Administration Modulates Central Neuropathic Pain Development After Spinal Cord Injury in Mice. Front Pharmacol. 2019;10:222 pubmed publisher
Tsai C, Kuo F, Lee S, Chang Y, Fu H. Endocytosis-dependent lysosomal degradation of Src induced by protease-activated receptor 1. FEBS Lett. 2019;593:504-517 pubmed publisher
Bunda S, Heir P, Metcalf J, Li A, Agnihotri S, Pusch S, et al. CIC protein instability contributes to tumorigenesis in glioblastoma. Nat Commun. 2019;10:661 pubmed publisher
Colle D, Santos D, de Souza V, Lopes M, Leal R, de Souza Brocardo P, et al. Sodium selenite protects from 3-nitropropionic acid-induced oxidative stress in cultured primary cortical neurons. Mol Biol Rep. 2019;46:751-762 pubmed publisher
Arbel Rubinstein T, Shahmoon S, Zigmond E, Etan T, Merenbakh Lamin K, Pasmanik Chor M, et al. Klotho suppresses colorectal cancer through modulation of the unfolded protein response. Oncogene. 2019;38:794-807 pubmed publisher
Rosenberg L, Cattin A, Fontana X, Harford Wright E, Burden J, White I, et al. HDAC3 Regulates the Transition to the Homeostatic Myelinating Schwann Cell State. Cell Rep. 2018;25:2755-2765.e5 pubmed publisher
Fernández Espartero C, Rizzo A, Fulford A, Falo Sanjuan J, Goutte Gattat D, Ribeiro P. Prp8 regulates oncogene-induced hyperplastic growth in Drosophila. Development. 2018;145: pubmed publisher
Del Rio C, Cantarero I, Palomares B, Gómez Cañas M, Fernandez Ruiz J, Pavicic C, et al. VCE-004.3, a cannabidiol aminoquinone derivative, prevents bleomycin-induced skin fibrosis and inflammation through PPARγ- and CB2 receptor-dependent pathways. Br J Pharmacol. 2018;175:3813-3831 pubmed publisher
Rukhlenko O, Khorsand F, Krstic A, Rožanc J, Alexopoulos L, Rauch N, et al. Dissecting RAF Inhibitor Resistance by Structure-based Modeling Reveals Ways to Overcome Oncogenic RAS Signaling. Cell Syst. 2018;7:161-179.e14 pubmed publisher
Duan L, Ye L, Zhuang L, Zou X, Liu S, Zhang Y, et al. VEGFC/VEGFR3 axis mediates TGFβ1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer cells. PLoS ONE. 2018;13:e0200452 pubmed publisher
Higa T, Takahashi H, Higa Nakamine S, Suzuki M, Yamamoto H. Up-regulation of DUSP5 and DUSP6 by gonadotropin-releasing hormone in cultured hypothalamic neurons, GT1-7 cells. Biomed Res. 2018;39:149-158 pubmed publisher
Rozenbaum M, Rajman M, Rishal I, Koppel I, Koley S, Medzihradszky K, et al. Translatome Regulation in Neuronal Injury and Axon Regrowth. Eneuro. 2018;5: pubmed publisher
Zahavi E, Steinberg N, Altman T, Chein M, Joshi Y, Gradus Pery T, et al. The receptor tyrosine kinase TrkB signals without dimerization at the plasma membrane. Sci Signal. 2018;11: pubmed publisher
Brohée L, Peulen O, Nusgens B, Castronovo V, Thiry M, Colige A, et al. Propranolol sensitizes prostate cancer cells to glucose metabolism inhibition and prevents cancer progression. Sci Rep. 2018;8:7050 pubmed publisher
Shiloh R, Gilad Y, Ber Y, Eisenstein M, Aweida D, Bialik S, et al. Non-canonical activation of DAPK2 by AMPK constitutes a new pathway linking metabolic stress to autophagy. Nat Commun. 2018;9:1759 pubmed publisher
Chen J, Cao Z, Guan J. SHP2 inhibitor PHPS1 protects against atherosclerosis by inhibiting smooth muscle cell proliferation. BMC Cardiovasc Disord. 2018;18:72 pubmed publisher
Majorini M, Manenti G, Mano M, De Cecco L, Conti A, Pinciroli P, et al. cIAP1 regulates the EGFR/Snai2 axis in triple-negative breast cancer cells. Cell Death Differ. 2018;25:2147-2164 pubmed publisher
Jia Y, Zhang C, Hu C, Yu Y, Zheng X, Li Y, et al. EGFR inhibition enhances the antitumor efficacy of a selective BRAF V600E inhibitor in thyroid cancer cell lines. Oncol Lett. 2018;15:6763-6769 pubmed publisher
Goyal R, Tulpan D, Chomistek N, González Peña Fundora D, West C, Ellis B, et al. Analysis of MAPK and MAPKK gene families in wheat and related Triticeae species. BMC Genomics. 2018;19:178 pubmed publisher
Castany S, Gris G, Vela J, Verdú E, Boadas Vaello P. Critical role of sigma-1 receptors in central neuropathic pain-related behaviours after mild spinal cord injury in mice. Sci Rep. 2018;8:3873 pubmed publisher
Zhou J, Hu Q, Zhang X, Zheng J, Xie B, Xu Z, et al. Sensitivity to chemotherapeutics of NSCLC cells with acquired resistance to EGFR-TKIs is mediated by T790M mutation or epithelial-mesenchymal transition. Oncol Rep. 2018;39:1783-1792 pubmed publisher
Zhou D, Fu H, Xiao L, Mo H, Zhuo H, Tian X, et al. Fibroblast-Specific β-Catenin Signaling Dictates the Outcome of AKI. J Am Soc Nephrol. 2018;29:1257-1271 pubmed publisher
Dong F, Liu T, Jin H, Wang W. Chimaphilin inhibits human osteosarcoma cell invasion and metastasis through suppressing the TGF-β1-induced epithelial-to-mesenchymal transition markers via PI-3K/Akt, ERK1/2, and Smad signaling pathways. Can J Physiol Pharmacol. 2018;96:1-7 pubmed publisher
Wilson C, Nimick M, Nehoff H, Ashton J. ALK and IGF-1R as independent targets in crizotinib resistant lung cancer. Sci Rep. 2017;7:13955 pubmed publisher
Wang Z, Zhang F, He J, Wu P, Tay L, Cai M, et al. Binding of PLD2-Generated Phosphatidic Acid to KIF5B Promotes MT1-MMP Surface Trafficking and Lung Metastasis of Mouse Breast Cancer Cells. Dev Cell. 2017;43:186-197.e7 pubmed publisher
Zhao J, Zhao J, Xu G, Wang Z, Gao J, Cui S, et al. Deletion of Spata2 by CRISPR/Cas9n causes increased inhibin alpha expression and attenuated fertility in male mice. Biol Reprod. 2017;97:497-513 pubmed publisher
Bluemn E, Coleman I, Lucas J, Coleman R, Hernández López S, Tharakan R, et al. Androgen Receptor Pathway-Independent Prostate Cancer Is Sustained through FGF Signaling. Cancer Cell. 2017;32:474-489.e6 pubmed publisher
Kuenzi B, Remsing Rix L, Stewart P, Fang B, Kinose F, Bryant A, et al. Polypharmacology-based ceritinib repurposing using integrated functional proteomics. Nat Chem Biol. 2017;13:1222-1231 pubmed publisher
Shih C, Chang Y, Huang W, Jang T, Kung H, Wang W, et al. EZH2-mediated upregulation of ROS1 oncogene promotes oral cancer metastasis. Oncogene. 2017;36:6542-6554 pubmed publisher
Innamorati G, Fontana E, Steccanella F, Gandhi K, Bassi G, Zandonà V, et al. Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology. Cell Death Dis. 2017;8:e2930 pubmed publisher
Yoon S, Kovalenko A, Bogdanov K, Wallach D. MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation. Immunity. 2017;47:51-65.e7 pubmed publisher
Agron M, Brekhman V, Morgenstern D, Lotan T. Regulation of AP-1 by MAPK Signaling in Metal-Stressed Sea Anemone. Cell Physiol Biochem. 2017;42:952-964 pubmed publisher
Gamage T, Anderson J, Abood M. CB1 allosteric modulator Org27569 is an antagonist/inverse agonist of ERK1/2 signaling. Cannabis Cannabinoid Res. 2016;1:272-280 pubmed publisher
Sobral L, Coletta R, Alberici L, Curti C, Leopoldino A. SET/I2PP2A overexpression induces phenotypic, molecular, and metabolic alterations in an oral keratinocyte cell line. FEBS J. 2017;284:2774-2785 pubmed publisher
Suisse A, He D, Legent K, Treisman J. COP9 signalosome subunits protect Capicua from MAPK-dependent and -independent mechanisms of degradation. Development. 2017;144:2673-2682 pubmed publisher
Rives Quinto N, Franco M, de Torres Jurado A, Carmena A. Synergism between canoe and scribble mutations causes tumor-like overgrowth via Ras activation in neural stem cells and epithelia. Development. 2017;144:2570-2583 pubmed publisher
Plössl K, Royer M, Bernklau S, Tavraz N, Friedrich T, Wild J, et al. Retinoschisin is linked to retinal Na/K-ATPase signaling and localization. Mol Biol Cell. 2017;28:2178-2189 pubmed publisher
Aquino Martínez R, Artigas N, Gámez B, Rosa J, Ventura F. Extracellular calcium promotes bone formation from bone marrow mesenchymal stem cells by amplifying the effects of BMP-2 on SMAD signalling. PLoS ONE. 2017;12:e0178158 pubmed publisher
Balczon R, Morrow K, Zhou C, Edmonds B, Alexeyev M, Pittet J, et al. Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J. 2017;31:2785-2796 pubmed publisher
Kwon S, An S, Kim M, Kim K, Choi Y, Ahn K, et al. Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo. Int J Mol Med. 2017;39:741-748 pubmed publisher
Duffy D, Krstic A, Halasz M, Schwarzl T, Konietzny A, Iljin K, et al. Retinoic acid and TGF-? signalling cooperate to overcome MYCN-induced retinoid resistance. Genome Med. 2017;9:15 pubmed publisher
Steinhauer J. Co-culture Activation of MAP Kinase in Drosophila S2 Cells. Methods Mol Biol. 2017;1487:235-241 pubmed
Wang Y, Li D, Luo J, Tian G, Zhao L, Liao D. Intrinsic cellular signaling mechanisms determine the sensitivity of cancer cells to virus-induced apoptosis. Sci Rep. 2016;6:37213 pubmed publisher
Rieger D, Navarro E, Buendia I, Parada E, González Lafuente L, Leon R, et al. ConBr, A Lectin Purified from the Seeds of Canavalia brasiliensis, Protects Against Ischemia in Organotypic Culture of Rat Hippocampus: Potential Implication of Voltage-Gated Calcium Channels. Neurochem Res. 2017;42:347-359 pubmed publisher
Hoffmann H, Crouzin N, Moreno E, Raivio N, Fuentes S, McCormick P, et al. Long-Lasting Impairment of mGluR5-Activated Intracellular Pathways in the Striatum After Withdrawal of Cocaine Self-Administration. Int J Neuropsychopharmacol. 2017;20:72-82 pubmed publisher
Fu H, Tian Y, Zhou L, Zhou D, Tan R, Stolz D, et al. Tenascin-C Is a Major Component of the Fibrogenic Niche in Kidney Fibrosis. J Am Soc Nephrol. 2017;28:785-801 pubmed publisher
Abramovitch Dahan C, Asraf H, Bogdanovic M, Sekler I, Bush A, Hershfinkel M. Amyloid ? attenuates metabotropic zinc sensing receptor, mZnR/GPR39, dependent Ca2+ , ERK1/2 and Clusterin signaling in neurons. J Neurochem. 2016;139:221-233 pubmed publisher
Kamei M, Yamashita K, Takahashi M, Fukumori F, Ichiishi A, Fujimura M. Involvement of MAK-1 and MAK-2 MAP kinases in cell wall integrity in Neurospora crassa. Biosci Biotechnol Biochem. 2016;80:1843-52 pubmed publisher
PETRUCCELLI E, Li Q, Rao Y, Kitamoto T. The Unique Dopamine/Ecdysteroid Receptor Modulates Ethanol-Induced Sedation in Drosophila. J Neurosci. 2016;36:4647-57 pubmed publisher
Komla Ebri D, Dambroise E, Kramer I, Benoist Lasselin C, Kaci N, Le Gall C, et al. Tyrosine kinase inhibitor NVP-BGJ398 functionally improves FGFR3-related dwarfism in mouse model. J Clin Invest. 2016;126:1871-84 pubmed publisher
Keravis T, Favot L, Abusnina A, Anton A, Justiniano H, Soleti R, et al. Delphinidin Inhibits Tumor Growth by Acting on VEGF Signalling in Endothelial Cells. PLoS ONE. 2015;10:e0145291 pubmed publisher
Bunda S, Burrell K, Heir P, Zeng L, Alamsahebpour A, Kano Y, et al. Inhibition of SHP2-mediated dephosphorylation of Ras suppresses oncogenesis. Nat Commun. 2015;6:8859 pubmed publisher
Gershoni Emek N, Mazza A, Chein M, Gradus Pery T, Xiang X, Li K, et al. Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1. Mol Cell Proteomics. 2016;15:506-22 pubmed publisher
Shukla P, Vogl C, Wallner B, Rigler D, Müller M, Macho Maschler S. High-throughput mRNA and miRNA profiling of epithelial-mesenchymal transition in MDCK cells. BMC Genomics. 2015;16:944 pubmed publisher
Higa Nakamine S, Maeda N, Toku S, Yamamoto H. Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone. J Biol Chem. 2015;290:25974-85 pubmed publisher
Jovasevic V, Corcoran K, Leaderbrand K, Yamawaki N, Guedea A, Chen H, et al. GABAergic mechanisms regulated by miR-33 encode state-dependent fear. Nat Neurosci. 2015;18:1265-71 pubmed publisher
Schmid T, Snoek L, Fröhli E, van der Bent M, Kammenga J, Hajnal A. Systemic Regulation of RAS/MAPK Signaling by the Serotonin Metabolite 5-HIAA. PLoS Genet. 2015;11:e1005236 pubmed publisher
Zhang X, Liang D, Chi Z, Chu Q, Zhao C, Ma R, et al. Effect of zinc on high glucose-induced epithelial-to-mesenchymal transition in renal tubular epithelial cells. Int J Mol Med. 2015;35:1747-54 pubmed publisher
Brohée L, Demine S, Willems J, Arnould T, Colige A, Deroanne C. Lipin-1 regulates cancer cell phenotype and is a potential target to potentiate rapamycin treatment. Oncotarget. 2015;6:11264-80 pubmed
Kondapalli K, Llongueras J, Capilla González V, Prasad H, Hack A, Smith C, et al. A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma. Nat Commun. 2015;6:6289 pubmed publisher
Zahavi E, Ionescu A, Gluska S, Gradus T, Ben Yaakov K, Perlson E. A compartmentalized microfluidic neuromuscular co-culture system reveals spatial aspects of GDNF functions. J Cell Sci. 2015;128:1241-52 pubmed publisher
Leibovich Rivkin T, Liubomirski Y, Meshel T, Abashidze A, Brisker D, Solomon H, et al. The inflammatory cytokine TNF? cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells. BMC Cancer. 2014;14:158 pubmed publisher
product information
Product No. :
M5670
Product Name :
Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit
Host :
rabbit
Conjugate :
unconjugated
Antibody Type :
polyclonal
Applications/Description :
Suitable for microarray; western blot: 1:40,000-1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast extract, respectively
Species Reactivity :
human, rat, mouse
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA