product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse
catalog :
M4401
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MY-21
reactivity :
human, dogs, chicken, bovine
application :
western blot, immunocytochemistry
more info or order :
citations: 73
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 3f
Shi X, Wen Z, Wang Y, Liu Y, Shi K, Jiu Y. Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration. Front Cell Dev Biol. 2021;9:665919 pubmed publisher
  • western blot; human; 1:100; loading ...; fig 6b
Fayad R, Rojas M, Partisani M, Finetti P, Dib S, Abélanet S, et al. EFA6B regulates a stop signal for collective invasion in breast cancer. Nat Commun. 2021;12:2198 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 6a
  • western blot; human; 1:1000; loading ...; fig 2a
Lehtimäki J, Fenix A, Kotila T, Balistreri G, Paavolainen L, Varjosalo M, et al. UNC-45a promotes myosin folding and stress fiber assembly. J Cell Biol. 2017;216:4053-4072 pubmed publisher
  • western blot; human; 1:1000; fig s4
  • western blot; dogs; 1:1000; fig 1
Tien S, Lee H, Yang Y, Lin M, Chen Y, Chang Z. The Shp2-induced epithelial disorganization defect is reversed by HDAC6 inhibition independent of Cdc42. Nat Commun. 2016;7:10420 pubmed publisher
  • western blot; chicken; 1:100; fig 6
Won G, Fudge D, Choh V. The effects of actomyosin disruptors on the mechanical integrity of the avian crystalline lens. Mol Vis. 2015;21:98-109 pubmed
Boyle S, Kular J, Nobis M, Ruszkiewicz A, Timpson P, Samuel M. Acute compressive stress activates RHO/ROCK-mediated cellular processes. Small Gtpases. 2020;11:354-370 pubmed publisher
Asano S, Ikura Y, Nishimoto M, Yamawaki Y, Hamao K, Kamijo K, et al. Phospholipase C-related catalytically inactive protein regulates cytokinesis by protecting phosphatidylinositol 4,5-bisphosphate from metabolism in the cleavage furrow. Sci Rep. 2019;9:12729 pubmed publisher
Godoy Parejo C, Deng C, Liu W, Chen G. Insulin Stimulates PI3K/AKT and Cell Adhesion to Promote the Survival of Individualized Human Embryonic Stem Cells. Stem Cells. 2019;37:1030-1041 pubmed publisher
Cao J, Yang T, Tang D, Zhou F, Qian Y, Zou X. Increased expression of GEF-H1 promotes colon cancer progression by RhoA signaling. Pathol Res Pract. 2019;215:1012-1019 pubmed publisher
Jiu Y, Kumari R, Fenix A, Schaible N, Liu X, Varjosalo M, et al. Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles. Curr Biol. 2019;29:81-92.e5 pubmed publisher
Sarker M, Aki S, Yoshioka K, Kuno K, Okamoto Y, Ishimaru K, et al. Class II PI3Ks α and β Are Required for Rho-Dependent Uterine Smooth Muscle Contraction and Parturition in Mice. Endocrinology. 2019;160:235-248 pubmed publisher
Tse A, Lee A, Takahashi N, Gong A, Kasai H, Tse F. Strong stimulation triggers full fusion exocytosis and very slow endocytosis of the small dense core granules in carotid glomus cells. J Neurogenet. 2018;32:267-278 pubmed publisher
Yuan W, Pepe Mooney B, Galli G, Dill M, Huang H, Hao M, et al. NUAK2 is a critical YAP target in liver cancer. Nat Commun. 2018;9:4834 pubmed publisher
Barnes E, Lee L, Barnes S, Brenner R, Alvira C, Cornfield D. β1-Subunit of the calcium-sensitive potassium channel modulates the pulmonary vascular smooth muscle cell response to hypoxia. Am J Physiol Lung Cell Mol Physiol. 2018;315:L265-L275 pubmed publisher
Kuo S, Chen C, Pan Y, Chiu W, Chen H. Biogenesis of podosome rosettes through fission. Sci Rep. 2018;8:524 pubmed publisher
Wang T, Hamilla S, Cam M, Aranda Espinoza H, Mili S. Extracellular matrix stiffness and cell contractility control RNA localization to promote cell migration. Nat Commun. 2017;8:896 pubmed publisher
Chaudhuri P, Rosenbaum M, Birnbaumer L, Graham L. Integration of TRPC6 and NADPH oxidase activation in lysophosphatidylcholine-induced TRPC5 externalization. Am J Physiol Cell Physiol. 2017;313:C541-C555 pubmed publisher
Silpanisong J, Kim D, Williams J, Adeoye O, Thorpe R, Pearce W. Chronic hypoxia alters fetal cerebrovascular responses to endothelin-1. Am J Physiol Cell Physiol. 2017;313:C207-C218 pubmed publisher
Chugh P, Clark A, Smith M, Cassani D, Dierkes K, Ragab A, et al. Actin cortex architecture regulates cell surface tension. Nat Cell Biol. 2017;19:689-697 pubmed publisher
Dogan M, Han Y, Delmotte P, Sieck G. TNF? enhances force generation in airway smooth muscle. Am J Physiol Lung Cell Mol Physiol. 2017;312:L994-L1002 pubmed publisher
Pfisterer S, Gateva G, Horvath P, Pirhonen J, Salo V, Karhinen L, et al. Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage. Nat Commun. 2017;8:14858 pubmed publisher
Jiu Y, Peranen J, Schaible N, Cheng F, Eriksson J, Krishnan R, et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci. 2017;130:892-902 pubmed publisher
Shiraishi A, Uruno T, Sanematsu F, Ushijima M, Sakata D, Hara T, et al. DOCK8 Protein Regulates Macrophage Migration through Cdc42 Protein Activation and LRAP35a Protein Interaction. J Biol Chem. 2017;292:2191-2202 pubmed publisher
Pearce W, Doan C, Carreon D, Kim D, Durrant L, Manaenko A, et al. Imatinib attenuates cerebrovascular injury and phenotypic transformation after intracerebral hemorrhage in rats. Am J Physiol Regul Integr Comp Physiol. 2016;311:R1093-R1104 pubmed publisher
Hanouni M, Bernal G, McBride S, Narvaez V, Ibe B. Hypoxia and hyperoxia potentiate PAF receptor-mediated effects in newborn ovine pulmonary arterial smooth muscle cells: significance in oxygen therapy of PPHN. Physiol Rep. 2016;4: pubmed publisher
Zhang L, Luga V, Armitage S, Musiol M, Won A, Yip C, et al. A lateral signalling pathway coordinates shape volatility during cell migration. Nat Commun. 2016;7:11714 pubmed publisher
Fenix A, Taneja N, Buttler C, Lewis J, Van Engelenburg S, Ohi R, et al. Expansion and concatenation of non-muscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis. Mol Biol Cell. 2016;: pubmed
Newell Litwa K, Badoual M, Asmussen H, Patel H, Whitmore L, Horwitz A. ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity. J Cell Biol. 2015;210:225-42 pubmed publisher
Parker W, Qu Z, May J. Intracellular Ascorbate Prevents Endothelial Barrier Permeabilization by Thrombin. J Biol Chem. 2015;290:21486-97 pubmed publisher
Supinski G, Alimov A, Wang L, Song X, Callahan L. Neutral sphingomyelinase 2 is required for cytokine-induced skeletal muscle calpain activation. Am J Physiol Lung Cell Mol Physiol. 2015;309:L614-24 pubmed publisher
Lu L, Sirish P, Zhang Z, Woltz R, Li N, Timofeyev V, et al. Regulation of gene transcription by voltage-gated L-type calcium channel, Cav1.3. J Biol Chem. 2015;290:4663-76 pubmed publisher
Borg M, Bakke O, Progida C. A novel interaction between Rab7b and actomyosin reveals a dual role in intracellular transport and cell migration. J Cell Sci. 2014;127:4927-39 pubmed publisher
Shutova M, Spessott W, Giraudo C, Svitkina T. Endogenous species of mammalian nonmuscle myosin IIA and IIB include activated monomers and heteropolymers. Curr Biol. 2014;24:1958-68 pubmed publisher
Bailon E, Ugarte Berzal E, Amigo Jiménez I, Van den Steen P, Opdenakker G, Garcia Marco J, et al. Overexpression of progelatinase B/proMMP-9 affects migration regulatory pathways and impairs chronic lymphocytic leukemia cell homing to bone marrow and spleen. J Leukoc Biol. 2014;96:185-99 pubmed publisher
Adeoye O, Bouthors V, Hubbell M, Williams J, Pearce W. VEGF receptors mediate hypoxic remodeling of adult ovine carotid arteries. J Appl Physiol (1985). 2014;117:777-87 pubmed publisher
Wong P, Roberts R, Randall M. Sex differences in endothelial function in porcine coronary arteries: a role for H2O2 and gap junctions?. Br J Pharmacol. 2014;171:2751-66 pubmed publisher
Artamonov M, Momotani K, Stevenson A, Trentham D, Derewenda U, Derewenda Z, et al. Agonist-induced Ca2+ sensitization in smooth muscle: redundancy of Rho guanine nucleotide exchange factors (RhoGEFs) and response kinetics, a caged compound study. J Biol Chem. 2013;288:34030-40 pubmed publisher
Adeoye O, Butler S, Hubbell M, Semotiuk A, Williams J, Pearce W. Contribution of increased VEGF receptors to hypoxic changes in fetal ovine carotid artery contractile proteins. Am J Physiol Cell Physiol. 2013;304:C656-65 pubmed publisher
Kovac B, Teo J, Makela T, Vallenius T. Assembly of non-contractile dorsal stress fibers requires ?-actinin-1 and Rac1 in migrating and spreading cells. J Cell Sci. 2013;126:263-73 pubmed publisher
Bielenberg D, Seth A, Shimizu A, Pelton K, Cristofaro V, Ramachandran A, et al. Increased smooth muscle contractility in mice deficient for neuropilin 2. Am J Pathol. 2012;181:548-59 pubmed publisher
Kakei T, Sumiyoshi H, Higashi Fujime S. Characteristics of light chains of Chara myosin revealed by immunological investigation. Proc Jpn Acad Ser B Phys Biol Sci. 2012;88:201-11 pubmed
Ahn J, Sanz Moreno V, Marshall C. The metastasis gene NEDD9 product acts through integrin ?3 and Src to promote mesenchymal motility and inhibit amoeboid motility. J Cell Sci. 2012;125:1814-26 pubmed publisher
Butler S, Abrassart J, Hubbell M, Adeoye O, Semotiuk A, Williams J, et al. Contributions of VEGF to age-dependent transmural gradients in contractile protein expression in ovine carotid arteries. Am J Physiol Cell Physiol. 2011;301:C653-66 pubmed publisher
Takagi T, Okamoto Y, Tomita S, Sato A, Yamaguchi S, Takuwa Y, et al. Intraradial administration of fasudil inhibits augmented Rho kinase activity to effectively dilate the spastic radial artery during coronary artery bypass grafting surgery. J Thorac Cardiovasc Surg. 2011;142:e59-65 pubmed publisher
Babayeva S, Miller M, Zilber Y, El Kares R, Bernard C, Bitzan M, et al. Plasma from a case of recurrent idiopathic FSGS perturbs non-muscle myosin IIA (MYH9 protein) in human podocytes. Pediatr Nephrol. 2011;26:1071-81 pubmed publisher
Vallenius T, Vaahtomeri K, Kovac B, Osiceanu A, Viljanen M, Makela T. An association between NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers. J Cell Sci. 2011;124:384-93 pubmed publisher
Vicente Manzanares M, Horwitz A. Myosin light chain mono- and di-phosphorylation differentially regulate adhesion and polarity in migrating cells. Biochem Biophys Res Commun. 2010;402:537-42 pubmed publisher
Seok Y, Azam M, Okamoto Y, Sato A, Yoshioka K, Maeda M, et al. Enhanced Ca2+-dependent activation of phosphoinositide 3-kinase class II? isoform-Rho axis in blood vessels of spontaneously hypertensive rats. Hypertension. 2010;56:934-41 pubmed publisher
Lee E, Peng J, Radke M, Gotthardt M, Granzier H. Calcium sensitivity and the Frank-Starling mechanism of the heart are increased in titin N2B region-deficient mice. J Mol Cell Cardiol. 2010;49:449-58 pubmed publisher
Guvenc B, Ustunel C, Ozturk N, Brozovich F. A dynamic approach reveals non-muscle myosin influences the overall smooth muscle cross-bridge cycling rate. FEBS Lett. 2010;584:2862-6 pubmed publisher
Maki J, Hirano M, Hoka S, Kanaide H, Hirano K. Thrombin activation of proteinase-activated receptor 1 potentiates the myofilament Ca2+ sensitivity and induces vasoconstriction in porcine pulmonary arteries. Br J Pharmacol. 2010;159:919-27 pubmed publisher
Yuen S, Ogut O, Brozovich F. Nonmuscle myosin is regulated during smooth muscle contraction. Am J Physiol Heart Circ Physiol. 2009;297:H191-9 pubmed publisher
Webb J, Murányi A, Pugh C, Ratcliffe P, Coleman M. MYPT1, the targeting subunit of smooth-muscle myosin phosphatase, is a substrate for the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH). Biochem J. 2009;420:327-33 pubmed publisher
Injeti E, Sandoval R, Williams J, Smolensky A, Ford L, Pearce W. Maximal stimulation-induced in situ myosin light chain kinase activity is upregulated in fetal compared with adult ovine carotid arteries. Am J Physiol Heart Circ Physiol. 2008;295:H2289-98 pubmed publisher
Fairbank N, Connolly S, Mackinnon J, Wehry K, Deng L, Maksym G. Airway smooth muscle cell tone amplifies contractile function in the presence of chronic cyclic strain. Am J Physiol Lung Cell Mol Physiol. 2008;295:L479-88 pubmed publisher
Chang Y, Nalbant P, Birkenfeld J, Chang Z, Bokoch G. GEF-H1 couples nocodazole-induced microtubule disassembly to cell contractility via RhoA. Mol Biol Cell. 2008;19:2147-53 pubmed publisher
Komatsu S, Ikebe M. The phosphorylation of myosin II at the Ser1 and Ser2 is critical for normal platelet-derived growth factor induced reorganization of myosin filaments. Mol Biol Cell. 2007;18:5081-90 pubmed
DeMali K, Jue A, Burridge K. IpaA targets beta1 integrins and rho to promote actin cytoskeleton rearrangements necessary for Shigella entry. J Biol Chem. 2006;281:39534-41 pubmed
Sturge J, Wienke D, Isacke C. Endosomes generate localized Rho-ROCK-MLC2-based contractile signals via Endo180 to promote adhesion disassembly. J Cell Biol. 2006;175:337-47 pubmed
Watanabe M, Oike M, Ohta Y, Nawata H, Ito Y. Sustained contraction and loss of NO production in TGFbeta1-treated endothelial cells. Br J Pharmacol. 2006;149:355-64 pubmed
Croft D, Olson M. The Rho GTPase effector ROCK regulates cyclin A, cyclin D1, and p27Kip1 levels by distinct mechanisms. Mol Cell Biol. 2006;26:4612-27 pubmed
Chang S, Hypolite J, Zderic S, Wein A, Chacko S, Disanto M. Increased corpus cavernosum smooth muscle tone associated with partial bladder outlet obstruction is mediated via Rho-kinase. Am J Physiol Regul Integr Comp Physiol. 2005;289:R1124-30 pubmed
Bogatcheva N, Verin A, Wang P, Birukova A, Birukov K, Mirzopoyazova T, et al. Phorbol esters increase MLC phosphorylation and actin remodeling in bovine lung endothelium without increased contraction. Am J Physiol Lung Cell Mol Physiol. 2003;285:L415-26 pubmed
Bryce N, Schevzov G, Ferguson V, Percival J, Lin J, Matsumura F, et al. Specification of actin filament function and molecular composition by tropomyosin isoforms. Mol Biol Cell. 2003;14:1002-16 pubmed
Li Y, Je H, Malek S, Morgan K. ERK1/2-mediated phosphorylation of myometrial caldesmon during pregnancy and labor. Am J Physiol Regul Integr Comp Physiol. 2003;284:R192-9 pubmed
Fladmark K, Brustugun O, Mellgren G, Krakstad C, Boe R, Vintermyr O, et al. Ca2+/calmodulin-dependent protein kinase II is required for microcystin-induced apoptosis. J Biol Chem. 2002;277:2804-11 pubmed
Khatri J, Joyce K, Brozovich F, Fisher S. Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation. J Biol Chem. 2001;276:37250-7 pubmed
Shimizu Y, Dobashi K, Iizuka K, Horie T, Suzuki K, Tukagoshi H, et al. Contribution of small GTPase Rho and its target protein rock in a murine model of lung fibrosis. Am J Respir Crit Care Med. 2001;163:210-7 pubmed
Hypolite J, DiSanto M, Zheng Y, Chang S, Wein A, Chacko S. Regional variation in myosin isoforms and phosphorylation at the resting tone in urinary bladder smooth muscle. Am J Physiol Cell Physiol. 2001;280:C254-64 pubmed
Fladmark K, Brustugun O, Hovland R, Boe R, Gjertsen B, Zhivotovsky B, et al. Ultrarapid caspase-3 dependent apoptosis induction by serine/threonine phosphatase inhibitors. Cell Death Differ. 1999;6:1099-108 pubmed
Redaelli G, Malhotra A, Li B, Li P, Sonnenblick E, Hofmann P, et al. Effects of constitutive overexpression of insulin-like growth factor-1 on the mechanical characteristics and molecular properties of ventricular myocytes. Circ Res. 1998;82:594-603 pubmed
Kirshenbaum L, Abdellatif M, Chakraborty S, Schneider M. Human E2F-1 reactivates cell cycle progression in ventricular myocytes and represses cardiac gene transcription. Dev Biol. 1996;179:402-11 pubmed
Eriksson J, Brautigan D, Vallee R, Olmsted J, Fujiki H, Goldman R. Cytoskeletal integrity in interphase cells requires protein phosphatase activity. Proc Natl Acad Sci U S A. 1992;89:11093-7 pubmed
product information
Product No. :
M4401
Product Name :
Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
MY-21
Applications/Description :
Suitable for indirect immunofluorescence: 1:200 using human or chicken fibroblasts; western blot using denatured and reduced myosin of muscle
Species Reactivity :
bovine, chicken, pig, rabbit, human
Isotype :
IgM
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA