product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-MDM2 antibody produced in mouse
catalog :
M4308
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SMP14
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunoprecipitation
more info or order :
citations: 23
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 1c
Liu T, Xiong J, Yi S, Zhang H, Zhou S, Gu L, et al. FKBP12 enhances sensitivity to chemotherapy-induced cancer cell apoptosis by inhibiting MDM2. Oncogene. 2017;36:1678-1686 pubmed publisher
Zhou Y, Wang L, Ban X, Zeng T, Zhu Y, Li M, et al. DHRS2 inhibits cell growth and motility in esophageal squamous cell carcinoma. Oncogene. 2017;: pubmed publisher
Chang T, Wang S, Chen L, Shih Y, Chang L, Liu S, et al. Regulation of the Abundance of Kaposi's Sarcoma-Associated Herpesvirus ORF50 Protein by Oncoprotein MDM2. PLoS Pathog. 2016;12:e1005918 pubmed publisher
Moussa R, Kovacevic Z, Richardson D. Differential targeting of the cyclin-dependent kinase inhibitor, p21CIP1/WAF1, by chelators with anti-proliferative activity in a range of tumor cell-types. Oncotarget. 2015;6:29694-711 pubmed publisher
Lezina L, Aksenova V, Fedorova O, Malikova D, Shuvalov O, Antonov A, et al. KMT Set7/9 affects genotoxic stress response via the Mdm2 axis. Oncotarget. 2015;6:25843-55 pubmed publisher
Payne L, Eves R, Jia L, Mak A. p53 Down regulates PDGF-induced formation of circular dorsal ruffles in rat aortic smooth muscle cells. PLoS ONE. 2014;9:e108257 pubmed publisher
Oliveira Alves M, da Mota Delgado A, Balducci I, Carvalho Y, Cavalcante A, Almeida J. Study of MDM2 and SUMO-1 expression in actinic cheilitis and lip cancer. Arch Dermatol Res. 2014;306:837-41 pubmed publisher
Hamilton G, Abraham A, Morton J, Sampson O, Pefani D, Khoronenkova S, et al. AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget. 2014;5:6142-67 pubmed
Kasim V, Huang C, Zhang J, Jia H, Wang Y, Yang L, et al. Synergistic cooperation of MDM2 and E2F1 contributes to TAp73 transcriptional activity. Biochem Biophys Res Commun. 2014;449:319-26 pubmed publisher
Iqbal N, Xu L, Devitt C, Skapek S. Isolation and characterization of mammalian cells expressing the Arf promoter during eye development. Biotechniques. 2014;56:239-49 pubmed publisher
Bista M, Petrovich M, Fersht A. MDMX contains an autoinhibitory sequence element. Proc Natl Acad Sci U S A. 2013;110:17814-9 pubmed publisher
Gonon G, Groetz J, De Toledo S, Howell R, Fromm M, Azzam E. Nontargeted stressful effects in normal human fibroblast cultures exposed to low fluences of high charge, high energy (HZE) particles: kinetics of biologic responses and significance of secondary radiations. Radiat Res. 2013;179:444-57 pubmed publisher
Lau R, Niu M, Pratt M. cIAP2 represses IKK?/?-mediated activation of MDM2 to prevent p53 degradation. Cell Cycle. 2012;11:4009-19 pubmed publisher
Oga A, Uchida K, Nakao M, Kawauchi S, Furuya T, Chochi Y, et al. Loss of 6q or 8p23 is associated with the total number of DNA copy number aberrations in adenoid cystic carcinoma. Oncol Rep. 2011;26:1393-8 pubmed publisher
Zhang L, Sun S, Zhou J, Liu J, Lv J, Yu X, et al. Knockdown of Akt1 promotes Akt2 upregulation and resistance to oxidative-stress-induced apoptosis through control of multiple signaling pathways. Antioxid Redox Signal. 2011;15:1-17 pubmed publisher
Mukhopadhyay U, Mooney P, Jia L, Eves R, Raptis L, Mak A. Doubles game: Src-Stat3 versus p53-PTEN in cellular migration and invasion. Mol Cell Biol. 2010;30:4980-95 pubmed publisher
Mukhopadhyay U, Eves R, Jia L, Mooney P, Mak A. p53 suppresses Src-induced podosome and rosette formation and cellular invasiveness through the upregulation of caldesmon. Mol Cell Biol. 2009;29:3088-98 pubmed publisher
Widenmaier S, Sampaio A, Underhill T, McIntosh C. Noncanonical activation of Akt/protein kinase B in {beta}-cells by the incretin hormone glucose-dependent insulinotropic polypeptide. J Biol Chem. 2009;284:10764-73 pubmed publisher
Shiraishi T, Nielsen P. Down-regulation of MDM2 and activation of p53 in human cancer cells by antisense 9-aminoacridine-PNA (peptide nucleic acid) conjugates. Nucleic Acids Res. 2004;32:4893-902 pubmed
Fujioka S, Schmidt C, Sclabas G, Li Z, Pelicano H, Peng B, et al. Stabilization of p53 is a novel mechanism for proapoptotic function of NF-kappaB. J Biol Chem. 2004;279:27549-59 pubmed
Picksley S, Vojtesek B, Sparks A, Lane D. Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides. Oncogene. 1994;9:2523-9 pubmed
Hickman E, Picksley S, Vousden K. Cells expressing HPV16 E7 continue cell cycle progression following DNA damage induced p53 activation. Oncogene. 1994;9:2177-81 pubmed
Martin K, Trouche D, Hagemeier C, Sørensen T, La Thangue N, Kouzarides T. Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein. Nature. 1995;375:691-4 pubmed
product information
Product No. :
M4308
Product Name :
Monoclonal Anti-MDM2 antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
SMP14
Applications/Description :
Suitable for immunocytochemistry; western blot: 1:1,000 using a whole extract of transfected 293T (human embryonal kidney) cells expressing human MDM2; microarray; indirect ELISA; immunoprecipitation (IP); immunohistochemistry (froze
Species Reactivity :
rat, human, mouse (weak)
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA