product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Anti-Myosin (Skeletal, Fast) antibody, Mouse monoclonal
catalog :
M1570
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MY-32
reactivity :
human, mouse, rat, chicken, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 40
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:5000; loading ...; fig s18f
Chakraborty A, Laukka T, Myllykoski M, Ringel A, Booker M, Tolstorukov M, et al. Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. Science. 2019;363:1217-1222 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s2b
Chang H, Kao C, Chung S, Chen W, Aninda L, Chen Y, et al. Bhlhe40 differentially regulates the function and number of peroxisomes and mitochondria in myogenic cells. Redox Biol. 2019;20:321-333 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3a
Cortez Toledo O, Schnair C, Sangngern P, Metzger D, Chao L. Nur77 deletion impairs muscle growth during developmental myogenesis and muscle regeneration in mice. PLoS ONE. 2017;12:e0171268 pubmed publisher
  • western blot; mouse; fig 2
Lambert M, Richard E, Duban Deweer S, Krzewinski F, Deracinois B, Dupont E, et al. O-GlcNAcylation is a key modulator of skeletal muscle sarcomeric morphometry associated to modulation of protein-protein interactions. Biochim Biophys Acta. 2016;1860:2017-30 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000; fig 5
Pourteymour S, Lee S, Langleite T, Eckardt K, Hjorth M, Bindesbøll C, et al. Perilipin 4 in human skeletal muscle: localization and effect of physical activity. Physiol Rep. 2015;3: pubmed publisher
  • immunocytochemistry; mouse; fig 5
Ohsawa Y, Takayama K, Nishimatsu S, Okada T, Fujino M, Fukai Y, et al. The Inhibitory Core of the Myostatin Prodomain: Its Interaction with Both Type I and II Membrane Receptors, and Potential to Treat Muscle Atrophy. PLoS ONE. 2015;10:e0133713 pubmed publisher
  • immunohistochemistry; mouse; 1:250; fig 3
Preuße K, Tveriakhina L, Schuster Gossler K, Gaspar C, Rosa A, Henrique D, et al. Context-Dependent Functional Divergence of the Notch Ligands DLL1 and DLL4 In Vivo. PLoS Genet. 2015;11:e1005328 pubmed publisher
Rhoads T, Clark J, Gustafson G, Miller K, Conklin M, DeMuth T, et al. Molecular and Functional Networks Linked to Sarcopenia Prevention by Caloric Restriction in Rhesus Monkeys. Cell Syst. 2020;10:156-168.e5 pubmed publisher
Chen X, Du W, Cai Z, Ji S, Dwivedi M, Chen J, et al. Uniaxial Stretching of Cell-Laden Microfibers for Promoting C2C12 Myoblasts Alignment and Myofibers Formation. ACS Appl Mater Interfaces. 2020;12:2162-2170 pubmed publisher
Guerrero M, Carmona G, Rodas G, Cadefau J, Maestro A, Cusso R. Assessment of muscle fiber adaptation in footballers using a new ELISA assay of myosin isoforms. J Sports Med Phys Fitness. 2019;59:1828-1834 pubmed publisher
Tenney A, Livet J, Belton T, Prochazkova M, Pearson E, Whitman M, et al. Etv1 Controls the Establishment of Non-overlapping Motor Innervation of Neighboring Facial Muscles during Development. Cell Rep. 2019;29:437-452.e4 pubmed publisher
Dang K, Gao Y, Yu H, Xu S, Jiang S, Zhang W, et al. Regular alteration of protein glycosylation in skeletal muscles of hibernating Daurian ground squirrels (Spermophilus dauricus). Comp Biochem Physiol B Biochem Mol Biol. 2019;237:110323 pubmed publisher
Findlay A, Bengoechea R, Pittman S, Chou T, True H, Weihl C. Lithium chloride corrects weakness and myopathology in a preclinical model of LGMD1D. Neurol Genet. 2019;5:e318 pubmed publisher
Wang Y, Hao L, Li H, Cleary J, Tomac M, Thapa A, et al. Abnormal nuclear aggregation and myotube degeneration in myotonic dystrophy type 1. Neurol Sci. 2019;40:1255-1265 pubmed publisher
Arhzaouy K, Papadopoulos C, Schulze N, Pittman S, Meyer H, Weihl C. VCP maintains lysosomal homeostasis and TFEB activity in differentiated skeletal muscle. Autophagy. 2019;:1-18 pubmed publisher
Capitanchik C, Dixon C, Swanson S, Florens L, Kerr A, Schirmer E. Analysis of RNA-Seq datasets reveals enrichment of tissue-specific splice variants for nuclear envelope proteins. Nucleus. 2018;9:410-430 pubmed publisher
Bi P, McAnally J, Shelton J, Sanchez Ortiz E, Bassel Duby R, Olson E. Fusogenic micropeptide Myomixer is essential for satellite cell fusion and muscle regeneration. Proc Natl Acad Sci U S A. 2018;115:3864-3869 pubmed publisher
Uchida T, Sakashita Y, Kitahata K, Yamashita Y, Tomida C, Kimori Y, et al. Reactive oxygen species upregulate expression of muscle atrophy-associated ubiquitin ligase Cbl-b in rat L6 skeletal muscle cells. Am J Physiol Cell Physiol. 2018;314:C721-C731 pubmed publisher
Akiyama T, Sato S, Chikazawa Nohtomi N, Soma A, Kimura H, Wakabayashi S, et al. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Sci Rep. 2018;8:1189 pubmed publisher
Huq E, Taylor A, Su Z, Wall C. Fiber type composition of epaxial muscles is geared toward facilitating rapid spinal extension in the leaper Galago senegalensis. Am J Phys Anthropol. 2018;166:95-106 pubmed publisher
Hammers D, Merscham Banda M, Hsiao J, ENGST S, Hartman J, Sweeney H. Supraphysiological levels of GDF11 induce striated muscle atrophy. EMBO Mol Med. 2017;9:531-544 pubmed publisher
Le Thanh P, Meinke P, Korfali N, Srsen V, Robson M, Wehnert M, et al. Immunohistochemistry on a panel of Emery-Dreifuss muscular dystrophy samples reveals nuclear envelope proteins as inconsistent markers for pathology. Neuromuscul Disord. 2017;27:338-351 pubmed publisher
Sakuma M, Gorski G, Sheu S, Lee S, Barrett L, Singh B, et al. Lack of motor recovery after prolonged denervation of the neuromuscular junction is not due to regenerative failure. Eur J Neurosci. 2016;43:451-62 pubmed publisher
Nogueira J, Hawrot K, Sharpe C, Noble A, Wood W, Jorge E, et al. The emergence of Pax7-expressing muscle stem cells during vertebrate head muscle development. Front Aging Neurosci. 2015;7:62 pubmed publisher
Anderson C, Hu J, Barnes R, Heidt A, Cornelissen I, Black B. Myocyte enhancer factor 2C function in skeletal muscle is required for normal growth and glucose metabolism in mice. Skelet Muscle. 2015;5:7 pubmed publisher
Imamura M, Chang B, Kohjima M, Li M, Hwang B, Taegtmeyer H, et al. MondoA deficiency enhances sprint performance in mice. Biochem J. 2014;464:35-48 pubmed publisher
Buck D, Smith J, Chung C, Ono Y, Sorimachi H, Labeit S, et al. Removal of immunoglobulin-like domains from titin's spring segment alters titin splicing in mouse skeletal muscle and causes myopathy. J Gen Physiol. 2014;143:215-30 pubmed publisher
Inman K, Purcell P, Kume T, Trainor P. Interaction between Foxc1 and Fgf8 during mammalian jaw patterning and in the pathogenesis of syngnathia. PLoS Genet. 2013;9:e1003949 pubmed publisher
Salanova M, Schiffl G, Gutsmann M, Felsenberg D, Furlan S, Volpe P, et al. Nitrosative stress in human skeletal muscle attenuated by exercise countermeasure after chronic disuse. Redox Biol. 2013;1:514-26 pubmed publisher
Weyers J, Schwartz S, Minami E, Carlson D, Dupras S, Weitz K, et al. Effects of cell grafting on coronary remodeling after myocardial infarction. J Am Heart Assoc. 2013;2:e000202 pubmed publisher
Ackermann M, Patel P, Valenti J, Takagi Y, Homsher E, Sellers J, et al. Loss of actomyosin regulation in distal arthrogryposis myopathy due to mutant myosin binding protein-C slow. FASEB J. 2013;27:3217-28 pubmed publisher
Suetta C, Frandsen U, Jensen L, Jensen M, Jespersen J, Hvid L, et al. Aging affects the transcriptional regulation of human skeletal muscle disuse atrophy. PLoS ONE. 2012;7:e51238 pubmed publisher
Matsumaru D, Haraguchi R, Miyagawa S, Motoyama J, Nakagata N, Meijlink F, et al. Genetic analysis of Hedgehog signaling in ventral body wall development and the onset of omphalocele formation. PLoS ONE. 2011;6:e16260 pubmed publisher
da Rocha S, Charalambous M, Lin S, Gutteridge I, Ito Y, Gray D, et al. Gene dosage effects of the imprinted delta-like homologue 1 (dlk1/pref1) in development: implications for the evolution of imprinting. PLoS Genet. 2009;5:e1000392 pubmed publisher
Holmberg J, Ingner G, Johansson C, Leander P, Hjalt T. PITX2 gain-of-function induced defects in mouse forelimb development. BMC Dev Biol. 2008;8:25 pubmed publisher
Bakkar N, Wang J, Ladner K, Wang H, Dahlman J, Carathers M, et al. IKK/NF-kappaB regulates skeletal myogenesis via a signaling switch to inhibit differentiation and promote mitochondrial biogenesis. J Cell Biol. 2008;180:787-802 pubmed publisher
Lara Pezzi E, Winn N, Paul A, McCullagh K, Slominsky E, Santini M, et al. A naturally occurring calcineurin variant inhibits FoxO activity and enhances skeletal muscle regeneration. J Cell Biol. 2007;179:1205-18 pubmed
Fornaro M, Burch P, Yang W, Zhang L, Hamilton C, Kim J, et al. SHP-2 activates signaling of the nuclear factor of activated T cells to promote skeletal muscle growth. J Cell Biol. 2006;175:87-97 pubmed
Horsley V, Friday B, Matteson S, Kegley K, Gephart J, Pavlath G. Regulation of the growth of multinucleated muscle cells by an NFATC2-dependent pathway. J Cell Biol. 2001;153:329-38 pubmed
Spencer J, Eliazer S, Ilaria R, Richardson J, Olson E. Regulation of microtubule dynamics and myogenic differentiation by MURF, a striated muscle RING-finger protein. J Cell Biol. 2000;150:771-84 pubmed
product information
Product No. :
M1570
Product Name :
Anti-Myosin (Skeletal, Fast) antibody, Mouse monoclonal
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
MY-32
Applications/Description :
Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using porcine tongue; western blot: 0.5-1.0 μg/mL using total extract of rabbit skeletal muscle; microarray
Species Reactivity :
chicken, rabbit, feline, mouse, rat, bovine, human, guinea pig
Isotype :
IgG1
Concentration :
~1.0 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA