product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal
catalog :
L0663
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
4H8-2
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
more info or order :
citations: 55
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:500; loading ...; fig 4c
Wang H, Marrosu E, Brayson D, Wasala N, Johnson E, Scott C, et al. Proteomic analysis identifies key differences in the cardiac interactomes of dystrophin and micro-dystrophin. Hum Mol Genet. 2021;30:1321-1336 pubmed publisher
  • immunohistochemistry; human; 1:400; loading ...; fig s1a
Reinhard J, Lin S, McKee K, Meinen S, Crosson S, Sury M, et al. Linker proteins restore basement membrane and correct LAMA2-related muscular dystrophy in mice. Sci Transl Med. 2017;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1d
McKee K, Crosson S, Meinen S, Reinhard J, Ruegg M, Yurchenco P. Chimeric protein repair of laminin polymerization ameliorates muscular dystrophy phenotype. J Clin Invest. 2017;127:1075-1089 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2
Dumont N, Rudnicki M. Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration. Methods Mol Biol. 2017;1560:179-188 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig s1b
Wang C, Wang M, Arrington J, Shan T, Yue F, Nie Y, et al. Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors. Development. 2017;144:235-247 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 6a
Paris N, Soroka A, Klose A, Liu W, Chakkalakal J. Smad4 restricts differentiation to promote expansion of satellite cell derived progenitors during skeletal muscle regeneration. elife. 2016;5: pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig s2b
Gautam J, Zhang X, Yao Y. The role of pericytic laminin in blood brain barrier integrity maintenance. Sci Rep. 2016;6:36450 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1a
Meehan D, Delimont D, Dufek B, Zallocchi M, Phillips G, Gratton M, et al. Endothelin-1 mediated induction of extracellular matrix genes in strial marginal cells underlies strial pathology in Alport mice. Hear Res. 2016;341:100-108 pubmed publisher
  • immunohistochemistry; mouse; fig 4
Dumont N, Wang Y, von Maltzahn J, Pasut A, Bentzinger C, Brun C, et al. Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division. Nat Med. 2015;21:1455-63 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig s5
Van Ry P, Wuebbles R, Key M, Burkin D. Galectin-1 Protein Therapy Prevents Pathology and Improves Muscle Function in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther. 2015;23:1285-1297 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:300; fig 3
Hausburg M, Doles J, Clement S, Cadwallader A, Hall M, Blackshear P, et al. Post-transcriptional regulation of satellite cell quiescence by TTP-mediated mRNA decay. elife. 2015;4:e03390 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Bernet J, Doles J, Hall J, Kelly Tanaka K, Carter T, Olwin B. p38 MAPK signaling underlies a cell-autonomous loss of stem cell self-renewal in skeletal muscle of aged mice. Nat Med. 2014;20:265-71 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 6d
Kim Y, Remacle A, Chernov A, Liu H, Shubayev I, Lai C, et al. The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration. PLoS ONE. 2012;7:e33664 pubmed publisher
  • immunohistochemistry; mouse
Prins K, Lowe D, Ervasti J. Skeletal muscle-specific ablation of gamma(cyto)-actin does not exacerbate the mdx phenotype. PLoS ONE. 2008;3:e2419 pubmed publisher
Gras S, Blasco A, Mòdol Caballero G, Tarabal O, Casanovas A, Piedrafita L, et al. Beneficial effects of dietary supplementation with green tea catechins and cocoa flavanols on aging-related regressive changes in the mouse neuromuscular system. Aging (Albany NY). 2021;13:18051-18093 pubmed publisher
Sansa A, Hidalgo I, Miralles M, de la Fuente S, Perez Garcia M, Munell F, et al. Spinal Muscular Atrophy autophagy profile is tissue-dependent: differential regulation between muscle and motoneurons. Acta Neuropathol Commun. 2021;9:122 pubmed publisher
Futaki S, Nakano I, Kawasaki M, Sanzen N, Sekiguchi K. Molecular profiling of the basement membrane of pluripotent epiblast cells in post-implantation stage mouse embryos. Regen Ther. 2019;12:55-65 pubmed publisher
Hauck J, Howard Z, Lowe J, Rastogi N, Pico M, Swager S, et al. Mineralocorticoid Receptor Signaling Contributes to Normal Muscle Repair After Acute Injury. Front Physiol. 2019;10:1324 pubmed publisher
Ehmsen J, Kawaguchi R, Mi R, Coppola G, Hoke A. Longitudinal RNA-Seq analysis of acute and chronic neurogenic skeletal muscle atrophy. Sci Data. 2019;6:179 pubmed publisher
Yue L, Talukder M, Gurjar A, Lee J, Noble M, Dirksen R, et al. 4-Aminopyridine attenuates muscle atrophy after sciatic nerve crush injury in mice. Muscle Nerve. 2019;60:192-201 pubmed publisher
Harish P, Malerba A, Lu Nguyen N, Forrest L, Cappellari O, Roth F, et al. Inhibition of myostatin improves muscle atrophy in oculopharyngeal muscular dystrophy (OPMD). J Cachexia Sarcopenia Muscle. 2019;10:1016-1026 pubmed publisher
Nirwane A, Johnson J, Nguyen B, Miner J, Yao Y. Mural cell-derived laminin-α5 plays a detrimental role in ischemic stroke. Acta Neuropathol Commun. 2019;7:23 pubmed publisher
Wang Y, Feige P, Brun C, Hekmatnejad B, Dumont N, Renaud J, et al. EGFR-Aurka Signaling Rescues Polarity and Regeneration Defects in Dystrophin-Deficient Muscle Stem Cells by Increasing Asymmetric Divisions. Cell Stem Cell. 2019;24:419-432.e6 pubmed publisher
Betts C, McClorey G, Healicon R, Hammond S, Manzano R, Muses S, et al. Cmah-dystrophin deficient mdx mice display an accelerated cardiac phenotype that is improved following peptide-PMO exon skipping treatment. Hum Mol Genet. 2019;28:396-406 pubmed publisher
Olthoff J, Lindsay A, Abo Zahrah R, Baltgalvis K, Patrinostro X, Belanto J, et al. Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun. 2018;9:5104 pubmed publisher
Bachman J, Klose A, Liu W, Paris N, Blanc R, Schmalz M, et al. Prepubertal skeletal muscle growth requires Pax7-expressing satellite cell-derived myonuclear contribution. Development. 2018;145: pubmed publisher
Bulaklak K, Xiao B, Qiao C, Li J, Patel T, Jin Q, et al. MicroRNA-206 Downregulation Improves Therapeutic Gene Expression and Motor Function in mdx Mice. Mol Ther Nucleic Acids. 2018;12:283-293 pubmed publisher
Filareto A, Maguire Nguyen K, Gan Q, Aldanondo G, Machado L, Chamberlain J, et al. Monitoring disease activity noninvasively in the mdx model of Duchenne muscular dystrophy. Proc Natl Acad Sci U S A. 2018;115:7741-7746 pubmed publisher
Blain A, Greally E, McClorey G, Manzano R, Betts C, Godfrey C, et al. Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy. PLoS ONE. 2018;13:e0198897 pubmed publisher
You J, Dooley M, Kim C, Kim E, Xu W, Goodman C, et al. A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling. Sci Signal. 2018;11: pubmed publisher
Koo T, Lu Nguyen N, Malerba A, Kim E, Kim D, Cappellari O, et al. Functional Rescue of Dystrophin Deficiency in Mice Caused by Frameshift Mutations Using Campylobacter jejuni Cas9. Mol Ther. 2018;26:1529-1538 pubmed publisher
Harada A, Maehara K, Ono Y, Taguchi H, Yoshioka K, Kitajima Y, et al. Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration. Nat Commun. 2018;9:1400 pubmed publisher
Nelson D, Lindsay A, Judge L, Duan D, Chamberlain J, Lowe D, et al. Variable rescue of microtubule and physiological phenotypes in mdx muscle expressing different miniaturized dystrophins. Hum Mol Genet. 2018;27:2090-2100 pubmed publisher
Jin Q, Qiao C, Li J, Li J, Xiao X. Neonatal Systemic AAV-Mediated Gene Delivery of GDF11 Inhibits Skeletal Muscle Growth. Mol Ther. 2018;26:1109-1117 pubmed publisher
Sciandra F, Scicchitano B, Signorino G, Bigotti M, Tavazzi B, Lombardi F, et al. Evaluation of the effect of a floxed Neo cassette within the dystroglycan (Dag1) gene. BMC Res Notes. 2017;10:601 pubmed publisher
Figlia G, Norrmen C, Pereira J, Gerber D, Suter U. Dual function of the PI3K-Akt-mTORC1 axis in myelination of the peripheral nervous system. elife. 2017;6: pubmed publisher
Malerba A, Klein P, Bachtarzi H, Jarmin S, Córdova G, Ferry A, et al. PABPN1 gene therapy for oculopharyngeal muscular dystrophy. Nat Commun. 2017;8:14848 pubmed publisher
Nakajima T, Wang B, Ono T, Uehara Y, Nakamura S, Ichinohe K, et al. Differences in sustained alterations in protein expression between livers of mice exposed to high-dose-rate and low-dose-rate radiation. J Radiat Res. 2017;58:421-429 pubmed publisher
Chenette D, Cadwallader A, Antwine T, Larkin L, Wang J, Olwin B, et al. Targeted mRNA Decay by RNA Binding Protein AUF1 Regulates Adult Muscle Stem Cell Fate, Promoting Skeletal Muscle Integrity. Cell Rep. 2016;16:1379-1390 pubmed publisher
Dufek B, Meehan D, Delimont D, Cheung L, Gratton M, Phillips G, et al. Endothelin A receptor activation on mesangial cells initiates Alport glomerular disease. Kidney Int. 2016;90:300-310 pubmed publisher
Tjondrokoesoemo A, Schips T, Sargent M, Vanhoutte D, Kanisicak O, Prasad V, et al. Cathepsin S Contributes to the Pathogenesis of Muscular Dystrophy in Mice. J Biol Chem. 2016;291:9920-8 pubmed publisher
Xu R, Singhal N, Serinagaoglu Y, Chandrasekharan K, Joshi M, Bauer J, et al. Deletion of Galgt2 (B4Galnt2) reduces muscle growth in response to acute injury and increases muscle inflammation and pathology in dystrophin-deficient mice. Am J Pathol. 2015;185:2668-84 pubmed publisher
Liu W, Wei LaPierre L, Klose A, Dirksen R, Chakkalakal J. Inducible depletion of adult skeletal muscle stem cells impairs the regeneration of neuromuscular junctions. elife. 2015;4: pubmed publisher
Godfrey C, Muses S, McClorey G, Wells K, Coursindel T, Terry R, et al. How much dystrophin is enough: the physiological consequences of different levels of dystrophin in the mdx mouse. Hum Mol Genet. 2015;24:4225-37 pubmed publisher
Hayashiji N, Yuasa S, Miyagoe Suzuki Y, Hara M, Ito N, Hashimoto H, et al. G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy. Nat Commun. 2015;6:6745 pubmed publisher
Arnett A, Konieczny P, Ramos J, Hall J, ODOM G, Yablonka Reuveni Z, et al. Adeno-associated viral (AAV) vectors do not efficiently target muscle satellite cells. Mol Ther Methods Clin Dev. 2014;1: pubmed
Delimont D, Dufek B, Meehan D, Zallocchi M, Gratton M, Phillips G, et al. Laminin ?2-mediated focal adhesion kinase activation triggers Alport glomerular pathogenesis. PLoS ONE. 2014;9:e99083 pubmed publisher
Yao Y, Chen Z, Norris E, Strickland S. Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nat Commun. 2014;5:3413 pubmed publisher
Roberts T, Godfrey C, McClorey G, Vader P, Briggs D, Gardiner C, et al. Extracellular microRNAs are dynamic non-vesicular biomarkers of muscle turnover. Nucleic Acids Res. 2013;41:9500-13 pubmed publisher
Roberts T, Blomberg K, McClorey G, El Andaloussi S, Godfrey C, Betts C, et al. Expression analysis in multiple muscle groups and serum reveals complexity in the microRNA transcriptome of the mdx mouse with implications for therapy. Mol Ther Nucleic Acids. 2012;1:e39 pubmed publisher
Singhal N, Xu R, Martin P. Distinct contributions of Galgt1 and Galgt2 to carbohydrate expression and function at the mouse neuromuscular junction. Mol Cell Neurosci. 2012;51:112-26 pubmed publisher
Baltgalvis K, Jaeger M, Fitzsimons D, Thayer S, Lowe D, Ervasti J. Transgenic overexpression of γ-cytoplasmic actin protects against eccentric contraction-induced force loss in mdx mice. Skelet Muscle. 2011;1:32 pubmed publisher
Allamand V, Sunada Y, Salih M, Straub V, Ozo C, Al Turaiki M, et al. Mild congenital muscular dystrophy in two patients with an internally deleted laminin alpha2-chain. Hum Mol Genet. 1997;6:747-52 pubmed
Schuler F, Sorokin L. Expression of laminin isoforms in mouse myogenic cells in vitro and in vivo. J Cell Sci. 1995;108 ( Pt 12):3795-805 pubmed
Sewry C, Philpot J, Sorokin L, Wilson L, Naom I, Goodwin F, et al. Diagnosis of merosin (laminin-2) deficient congenital muscular dystrophy by skin biopsy. Lancet. 1996;347:582-4 pubmed
product information
Product No. :
L0663
Product Name :
Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal
Host :
rat
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
4H8-2
Applications/Description :
Suitable for immunocytochemistry; western blot; microarray; indirect immunofluorescence: 4-8 μg/mL using acetone-fixed frozen sections of human tongue; indirect ELISA; immunoprecipitation (IP); immunohistochemistry
Species Reactivity :
human, mouse
Isotype :
IgG1
Concentration :
~2 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA