product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Anti-MCU antibody produced in rabbit
catalog :
HPA016480
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 65
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 1c
Paggio A, Checchetto V, Campo A, Menabò R, Di Marco G, Di Lisa F, et al. Identification of an ATP-sensitive potassium channel in mitochondria. Nature. 2019;572:609-613 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig s1a
Wang X, Zhu Y, Long H, Pan S, Xiong H, Fang Q, et al. Mitochondrial Calcium Transporters Mediate Sensitivity to Noise-Induced Losses of Hair Cells and Cochlear Synapses. Front Mol Neurosci. 2018;11:469 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1b
Luongo T, Lambert J, Gross P, Nwokedi M, Lombardi A, Shanmughapriya S, et al. The mitochondrial Na+/Ca2+ exchanger is essential for Ca2+ homeostasis and viability. Nature. 2017;545:93-97 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 1d
  • immunoprecipitation; mouse; fig 3a
Tsai C, Wu Y, Pao P, Phillips C, Williams C, Miller C, et al. Proteolytic control of the mitochondrial calcium uniporter complex. Proc Natl Acad Sci U S A. 2017;114:4388-4393 pubmed publisher
  • western blot; human; fig 1b
Sugiura A, Mattie S, Prudent J, McBride H. Newly born peroxisomes are a hybrid of mitochondrial and ER-derived pre-peroxisomes. Nature. 2017;542:251-254 pubmed publisher
  • western blot; rat; loading ...; fig 3b
Kim T, Terentyeva R, Roder K, Li W, Liu M, Greener I, et al. SK channel enhancers attenuate Ca2+-dependent arrhythmia in hypertrophic hearts by regulating mito-ROS-dependent oxidation and activity of RyR. Cardiovasc Res. 2017;113:343-353 pubmed publisher
  • western blot; mouse; loading ...; fig 1e
Liu J, Liu J, Holmström K, Menazza S, Parks R, Fergusson M, et al. MICU1 Serves as a Molecular Gatekeeper to Prevent In Vivo Mitochondrial Calcium Overload. Cell Rep. 2016;16:1561-1573 pubmed publisher
  • western blot; human; 1:500; fig s2
Vais H, Mallilankaraman K, Mak D, Hoff H, Payne R, Tanis J, et al. EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. Cell Rep. 2016;14:403-410 pubmed publisher
  • western blot; mouse; fig 1
Marland J, Hasel P, Bonnycastle K, Cousin M. Mitochondrial Calcium Uptake Modulates Synaptic Vesicle Endocytosis in Central Nerve Terminals. J Biol Chem. 2016;291:2080-6 pubmed publisher
Calvo Rodríguez M, Hou S, Snyder A, Kharitonova E, Russ A, Das S, et al. Increased mitochondrial calcium levels associated with neuronal death in a mouse model of Alzheimer's disease. Nat Commun. 2020;11:2146 pubmed publisher
Di Marco G, Vallese F, Jourde B, Bergsdorf C, Sturlese M, De Mario A, et al. A High-Throughput Screening Identifies MICU1 Targeting Compounds. Cell Rep. 2020;30:2321-2331.e6 pubmed publisher
N Belosludtsev K, V Dubinin M, Yu Talanov E, S Starinets V, S Tenkov K, M Zakharova N, et al. Transport of Ca2+ and Ca2+-Dependent Permeability Transition in the Liver and Heart Mitochondria of Rats with Different Tolerance to Acute Hypoxia. Biomolecules. 2020;10: pubmed publisher
Debattisti V, Horn A, Singh R, Seifert E, Hogarth M, Mazala D, et al. Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1. Cell Rep. 2019;29:1274-1286.e6 pubmed publisher
Norante R, Peggion C, Rossi D, Martorana F, De Mario A, Lia A, et al. ALS-Associated SOD1(G93A) Decreases SERCA Pump Levels and Increases Store-Operated Ca2+ Entry in Primary Spinal Cord Astrocytes from a Transgenic Mouse Model. Int J Mol Sci. 2019;20: pubmed publisher
Wiersma M, van Marion D, Wüst R, Houtkooper R, Zhang D, Groot N, et al. Mitochondrial Dysfunction Underlies Cardiomyocyte Remodeling in Experimental and Clinical Atrial Fibrillation. Cells. 2019;8: pubmed publisher
Lombardi A, Gibb A, Arif E, Kolmetzky D, Tomar D, Luongo T, et al. Mitochondrial calcium exchange links metabolism with the epigenome to control cellular differentiation. Nat Commun. 2019;10:4509 pubmed publisher
Gherardi G, Di Marco G, Rizzuto R, Mammucari C. Crosstalk between Mitochondrial Ca2+ Uptake and Autophagy in Skeletal Muscle. Oxid Med Cell Longev. 2019;2019:1845321 pubmed publisher
Kowaltowski A, Menezes Filho S, Assali E, Gonçalves I, Cabral Costa J, Abreu P, et al. Mitochondrial morphology regulates organellar Ca2+ uptake and changes cellular Ca2+ homeostasis. FASEB J. 2019;33:13176-13188 pubmed publisher
Niemann J, Zehm C, Waterstradt R, Tiedge M, Baltrusch S. Cytosolic and mitochondrial Ca2+ concentrations in primary hepatocytes change with ageing and in consequence of an mtDNA mutation. Cell Calcium. 2019;82:102055 pubmed publisher
Pulli I, Lof C, Blom T, Asghar M, Lassila T, Back N, et al. Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca2+ handling in HeLa cells. Biochim Biophys Acta Mol Cell Res. 2019;: pubmed publisher
Carreras Sureda A, Jaña F, Urra H, Durand S, Mortenson D, Sagredo A, et al. Non-canonical function of IRE1α determines mitochondria-associated endoplasmic reticulum composition to control calcium transfer and bioenergetics. Nat Cell Biol. 2019;: pubmed publisher
Wang Y, Nguyen N, She J, Zeng W, Yang Y, Bai X, et al. Structural Mechanism of EMRE-Dependent Gating of the Human Mitochondrial Calcium Uniporter. Cell. 2019;: pubmed publisher
Bækkerud F, Salerno S, Ceriotti P, Morland C, Storm Mathisen J, Bergersen L, et al. High Intensity Interval Training Ameliorates Mitochondrial Dysfunction in the Left Ventricle of Mice with Type 2 Diabetes. Cardiovasc Toxicol. 2019;: pubmed publisher
Tomar D, Jaña F, Dong Z, Quinn W, Jadiya P, Breves S, et al. Blockade of MCU-Mediated Ca2+ Uptake Perturbs Lipid Metabolism via PP4-Dependent AMPK Dephosphorylation. Cell Rep. 2019;26:3709-3725.e7 pubmed publisher
Fedeli C, Filadi R, Rossi A, Mammucari C, Pizzo P. PSEN2 (presenilin 2) mutants linked to familial Alzheimer disease impair autophagy by altering Ca2+ homeostasis. Autophagy. 2019;15:2044-2062 pubmed publisher
Wei LaPierre L, Ainbinder A, Tylock K, Dirksen R. Substrate-dependent and cyclophilin D-independent regulation of mitochondrial flashes in skeletal and cardiac muscle. Arch Biochem Biophys. 2019;665:122-131 pubmed publisher
Miller B, Wang J, Song J, Zhang X, Hirschler Laszkiewicz I, Shanmughapriya S, et al. Trpm2 enhances physiological bioenergetics and protects against pathological oxidative cardiac injury: Role of Pyk2 phosphorylation. J Cell Physiol. 2019;: pubmed publisher
Liu M, Jeong E, Liu H, Xie A, So E, Shi G, et al. Magnesium supplementation improves diabetic mitochondrial and cardiac diastolic function. JCI Insight. 2019;4: pubmed publisher
Zhao M, Chen J, Mao K, She H, Ren Y, Gui C, et al. Mitochondrial calcium dysfunction contributes to autophagic cell death induced by MPP+ via AMPK pathway. Biochem Biophys Res Commun. 2019;509:390-394 pubmed publisher
Marchi S, Corricelli M, Branchini A, Vitto V, Missiroli S, Morciano G, et al. Akt-mediated phosphorylation of MICU1 regulates mitochondrial Ca2+ levels and tumor growth. EMBO J. 2019;38: pubmed publisher
Chen Q, Tang L, Xin G, Li S, Ma L, Xu Y, et al. Oxidative stress mediated by lipid metabolism contributes to high glucose-induced senescence in retinal pigment epithelium. Free Radic Biol Med. 2019;130:48-58 pubmed publisher
Roest G, Hesemans E, Welkenhuyzen K, Luyten T, Engedal N, Bultynck G, et al. The ER Stress Inducer l-Azetidine-2-Carboxylic Acid Elevates the Levels of Phospho-eIF2α and of LC3-II in a Ca2+-Dependent Manner. Cells. 2018;7: pubmed publisher
Wettmarshausen J, Goh V, Huang K, Arduino D, Tripathi U, Leimpek A, et al. MICU1 Confers Protection from MCU-Dependent Manganese Toxicity. Cell Rep. 2018;25:1425-1435.e7 pubmed publisher
Angebault C, Fauconnier J, Patergnani S, Rieusset J, Danese A, Affortit C, et al. ER-mitochondria cross-talk is regulated by the Ca2+ sensor NCS1 and is impaired in Wolfram syndrome. Sci Signal. 2018;11: pubmed publisher
Shanmughapriya S, Tomar D, Dong Z, Slovik K, Nemani N, Natarajaseenivasan K, et al. FOXD1-dependent MICU1 expression regulates mitochondrial activity and cell differentiation. Nat Commun. 2018;9:3449 pubmed publisher
Kamer K, Sancak Y, Fomina Y, Meisel J, Chaudhuri D, Grabarek Z, et al. MICU1 imparts the mitochondrial uniporter with the ability to discriminate between Ca2+ and Mn2+. Proc Natl Acad Sci U S A. 2018;115:E7960-E7969 pubmed publisher
Xu H, Guan N, Ren Y, Wei Q, Tao Y, Yang G, et al. IP3R-Grp75-VDAC1-MCU calcium regulation axis antagonists protect podocytes from apoptosis and decrease proteinuria in an Adriamycin nephropathy rat model. BMC Nephrol. 2018;19:140 pubmed publisher
Yu Z, Chen R, Li M, Yu Y, Liang Y, Han F, et al. Mitochondrial calcium uniporter inhibition provides cardioprotection in pressure overload-induced heart failure through autophagy enhancement. Int J Cardiol. 2018;271:161-168 pubmed publisher
Xie A, Song Z, Liu H, Zhou A, Shi G, Wang Q, et al. Mitochondrial Ca2+ Influx Contributes to Arrhythmic Risk in Nonischemic Cardiomyopathy. J Am Heart Assoc. 2018;7: pubmed publisher
Suarez J, Cividini F, Scott B, Lehmann K, Díaz Juárez J, Diemer T, et al. Restoring mitochondrial calcium uniporter expression in diabetic mouse heart improves mitochondrial calcium handling and cardiac function. J Biol Chem. 2018;293:8182-8195 pubmed publisher
Zeng F, Chen X, Cui W, Wen W, Lu F, Sun X, et al. RIPK1 Binds MCU to Mediate Induction of Mitochondrial Ca2+ Uptake and Promotes Colorectal Oncogenesis. Cancer Res. 2018;78:2876-2885 pubmed publisher
Pan M, Han Y, Basu A, Dai A, Si R, Willson C, et al. Overexpression of hexokinase 2 reduces mitochondrial calcium overload in coronary endothelial cells of type 2 diabetic mice. Am J Physiol Cell Physiol. 2018;314:C732-C740 pubmed publisher
Sebag S, Koval O, Paschke J, Winters C, Comellas A, Grumbach I. Inhibition of the mitochondrial calcium uniporter prevents IL-13 and allergen-mediated airway epithelial apoptosis and loss of barrier function. Exp Cell Res. 2018;362:400-411 pubmed publisher
Chakrabarti R, Ji W, Stan R, de Juan Sanz J, Ryan T, Higgs H. INF2-mediated actin polymerization at the ER stimulates mitochondrial calcium uptake, inner membrane constriction, and division. J Cell Biol. 2018;217:251-268 pubmed publisher
Yu C, Wang Y, Peng J, Shen Q, Chen M, Tang W, et al. Mitochondrial calcium uniporter as a target of microRNA-340 and promoter of metastasis via enhancing the Warburg effect. Oncotarget. 2017;8:83831-83844 pubmed publisher
Huang E, Qu D, Huang T, Rizzi N, Boonying W, Krolak D, et al. PINK1-mediated phosphorylation of LETM1 regulates mitochondrial calcium transport and protects neurons against mitochondrial stress. Nat Commun. 2017;8:1399 pubmed publisher
Verma M, Callio J, Otero P, Sekler I, Wills Z, Chu C. Mitochondrial Calcium Dysregulation Contributes to Dendrite Degeneration Mediated by PD/LBD-Associated LRRK2 Mutants. J Neurosci. 2017;37:11151-11165 pubmed publisher
Hernández Morales M, Sobradillo D, Valero R, Munoz E, Ubierna D, Moyer M, et al. Mitochondria sustain store-operated currents in colon cancer cells but not in normal colonic cells: reversal by non-steroidal anti-inflammatory drugs. Oncotarget. 2017;8:55332-55352 pubmed publisher
Kiefmann M, Tank S, Keller P, Börnchen C, Rinnenthal J, Tritt M, et al. IDH3 mediates apoptosis of alveolar epithelial cells type 2 due to mitochondrial Ca2+ uptake during hypocapnia. Cell Death Dis. 2017;8:e3005 pubmed publisher
Arduino D, Wettmarshausen J, Vais H, Navas Navarro P, Cheng Y, Leimpek A, et al. Systematic Identification of MCU Modulators by Orthogonal Interspecies Chemical Screening. Mol Cell. 2017;67:711-723.e7 pubmed publisher
Chen W, Yang J, Chen S, Xiang H, Liu H, Lin D, et al. Importance of mitochondrial calcium uniporter in high glucose-induced endothelial cell dysfunction. Diab Vasc Dis Res. 2017;14:494-501 pubmed publisher
Kamer K, Grabarek Z, Mootha V. High-affinity cooperative Ca2+ binding by MICU1-MICU2 serves as an on-off switch for the uniporter. EMBO Rep. 2017;18:1397-1411 pubmed publisher
Cho B, Cho H, Jo Y, Kim H, Song M, Moon C, et al. Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division. Nat Commun. 2017;8:15754 pubmed publisher
Eshima H, Miura S, Senoo N, Hatakeyama K, Poole D, Kano Y. Improved skeletal muscle Ca2+ regulation in vivo following contractions in mice overexpressing PGC-1α. Am J Physiol Regul Integr Comp Physiol. 2017;312:R1017-R1028 pubmed publisher
Qiao X, Jia S, Ye J, Fang X, Zhang C, Cao Y, et al. PTPIP51 regulates mouse cardiac ischemia/reperfusion through mediating the mitochondria-SR junction. Sci Rep. 2017;7:45379 pubmed publisher
Zampieri S, Mammucari C, Romanello V, Barberi L, Pietrangelo L, Fusella A, et al. Physical exercise in aging human skeletal muscle increases mitochondrial calcium uniporter expression levels and affects mitochondria dynamics. Physiol Rep. 2016;4: pubmed publisher
Naon D, Zaninello M, Giacomello M, Varanita T, Grespi F, Lakshminaranayan S, et al. Critical reappraisal confirms that Mitofusin 2 is an endoplasmic reticulum-mitochondria tether. Proc Natl Acad Sci U S A. 2016;113:11249-11254 pubmed
de la Fuente S, Fernandez Sanz C, Vail C, Agra E, Holmström K, Sun J, et al. Strategic Positioning and Biased Activity of the Mitochondrial Calcium Uniporter in Cardiac Muscle. J Biol Chem. 2016;291:23343-23362 pubmed
Tosatto A, Sommaggio R, Kummerow C, Bentham R, Blacker T, Berecz T, et al. The mitochondrial calcium uniporter regulates breast cancer progression via HIF-1α. EMBO Mol Med. 2016;8:569-85 pubmed publisher
Tsai M, Phillips C, Ranaghan M, Tsai C, Wu Y, Willliams C, et al. Dual functions of a small regulatory subunit in the mitochondrial calcium uniporter complex. elife. 2016;5: pubmed publisher
Zhang J, Wang C, Chen X, Takada M, Fan C, Zheng X, et al. EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer. EMBO J. 2015;34:2953-70 pubmed publisher
Lee Y, Min C, Kim T, Song H, Lim Y, Kim D, et al. Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter. EMBO Rep. 2015;16:1318-33 pubmed publisher
Patergnani S, Giorgi C, Maniero S, Missiroli S, Maniscalco P, Bononi I, et al. The endoplasmic reticulum mitochondrial calcium cross talk is downregulated in malignant pleural mesothelioma cells and plays a critical role in apoptosis inhibition. Oncotarget. 2015;6:23427-44 pubmed
Tang S, Wang X, Shen Q, Yang X, Yu C, Cai C, et al. Mitochondrial Ca²⁺ uniporter is critical for store-operated Ca²⁺ entry-dependent breast cancer cell migration. Biochem Biophys Res Commun. 2015;458:186-93 pubmed publisher
Quan X, Nguyen T, Choi S, Xu S, Das R, Cha S, et al. Essential role of mitochondrial Ca2+ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells. J Biol Chem. 2015;290:4086-96 pubmed publisher
product information
Product No. :
HPA016480
Product Name :
Anti-MCU antibody produced in rabbit
Host :
rabbit
Conjugate :
unconjugated
Antibody Type :
polyclonal
Applications/Description :
Suitable for immunoblotting: 0.04-0.4 μg/mL; immunohistochemistry: 1:50-1:200; immunofluorescence: 0.25-2 μg/mL
Species Reactivity :
human
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA