product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Anti-CDKL5 antibody produced in rabbit
catalog :
HPA002847
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, western blot knockout validation
more info or order :
citations: 8
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2a
Baltussen L, Negraes P, Silvestre M, Claxton S, Moeskops M, Christodoulou E, et al. Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics. EMBO J. 2018;37: pubmed publisher
  • immunocytochemistry; mouse; fig 2
  • western blot; mouse; fig 3
Nawaz M, Giarda E, Bedogni F, La Montanara P, Ricciardi S, Ciceri D, et al. CDKL5 and Shootin1 Interact and Concur in Regulating Neuronal Polarization. PLoS ONE. 2016;11:e0148634 pubmed publisher
Balestra D, Giorgio D, Bizzotto M, Fazzari M, Ben Zeev B, Pinotti M, et al. Splicing Mutations Impairing CDKL5 Expression and Activity Can be Efficiently Rescued by U1snRNA-Based Therapy. Int J Mol Sci. 2019;20: pubmed publisher
Fazzari M, Frasca A, Bifari F, Landsberger N. Aminoglycoside drugs induce efficient read-through of CDKL5 nonsense mutations, slightly restoring its kinase activity. RNA Biol. 2019;16:1414-1423 pubmed publisher
Tramarin M, Rusconi L, Pizzamiglio L, Barbiero I, Peroni D, Scaramuzza L, et al. The antidepressant tianeptine reverts synaptic AMPA receptor defects caused by deficiency of CDKL5. Hum Mol Genet. 2018;27:2052-2063 pubmed publisher
Barbiero I, Valente D, Chandola C, Magi F, Bergo A, Monteonofrio L, et al. CDKL5 localizes at the centrosome and midbody and is required for faithful cell division. Sci Rep. 2017;7:6228 pubmed publisher
Barbiero I, Peroni D, Tramarin M, Chandola C, Rusconi L, Landsberger N, et al. The neurosteroid pregnenolone reverts microtubule derangement induced by the loss of a functional CDKL5-IQGAP1 complex. Hum Mol Genet. 2017;26:3520-3530 pubmed publisher
La Montanara P, Rusconi L, Locarno A, Forti L, Barbiero I, Tramarin M, et al. Synaptic synthesis, dephosphorylation, and degradation: a novel paradigm for an activity-dependent neuronal control of CDKL5. J Biol Chem. 2015;290:4512-27 pubmed publisher
product information
Product No. :
HPA002847
Product Name :
Anti-CDKL5 antibody produced in rabbit
Host :
rabbit
Conjugate :
unconjugated
Antibody Type :
polyclonal
Applications/Description :
Suitable for immunofluorescence: 0.25-2 μg/mL; western blot: 0.04-0.4 μg/mL; immunohistochemistry: 1:200-1:500
Species Reactivity :
human
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA