product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-phospho-Histone H3 (pSer 28 ) antibody produced in rat
catalog :
H9908
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
HTA28
reactivity :
Planorbella trivolvis, American tobacco, human, mouse, bovine, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 42
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:1500; fig 1
Powell E, Shao J, Yuan Y, Chen H, Cai S, Echeverria G, et al. p53 deficiency linked to B cell translocation gene 2 (BTG2) loss enhances metastatic potential by promoting tumor growth in primary and metastatic sites in patient-derived xenograft (PDX) models of triple-negative breast cancer. Breast Cancer Res. 2016;18:13 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 7
Connor A, Kelley P, Tempero R. Lymphatic endothelial lineage assemblage during corneal lymphangiogenesis. Lab Invest. 2016;96:270-82 pubmed publisher
  • immunocytochemistry; American tobacco; 1:200; fig 5
Mursalimov S, Permyakova N, Deineko E, Houben A, Demidov D. Cytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes. Front Plant Sci. 2015;6:846 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 2
O Shaughnessy Kirwan A, Signolet J, Costello I, Gharbi S, Hendrich B. Constraint of gene expression by the chromatin remodelling protein CHD4 facilitates lineage specification. Development. 2015;142:2586-97 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig 1
Yin Y, Castro A, Hoekstra M, Yan T, Kanakamedala A, Dehner L, et al. Fibroblast Growth Factor 9 Regulation by MicroRNAs Controls Lung Development and Links DICER1 Loss to the Pathogenesis of Pleuropulmonary Blastoma. PLoS Genet. 2015;11:e1005242 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 5
Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun. 2015;6:6366 pubmed publisher
  • immunohistochemistry; mouse
Fink D, Connor A, Kelley P, Steele M, Hollingsworth M, Tempero R. Nerve growth factor regulates neurolymphatic remodeling during corneal inflammation and resolution. PLoS ONE. 2014;9:e112737 pubmed publisher
  • immunocytochemistry; human
Zhang X, Ling J, Barcia G, Jing L, Wu J, Barry B, et al. Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures. Am J Hum Genet. 2014;94:547-58 pubmed publisher
  • immunocytochemistry; Planorbella trivolvis; 1:1000
Glebov K, Voronezhskaya E, Khabarova M, Ivashkin E, Nezlin L, Ponimaskin E. Mechanisms underlying dual effects of serotonin during development of Helisoma trivolvis (Mollusca). BMC Dev Biol. 2014;14:14 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100
Douglas N, Arora R, Chen C, Sauer M, Papaioannou V. Investigating the role of tbx4 in the female germline in mice. Biol Reprod. 2013;89:148 pubmed publisher
  • western blot; human
Lau P, Cheung P. Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity. Nucleic Acids Res. 2013;41:e49 pubmed publisher
  • immunohistochemistry; fruit fly
Wartlick O, Mumcu P, Kicheva A, Bittig T, Seum C, Jülicher F, et al. Dynamics of Dpp signaling and proliferation control. Science. 2011;331:1154-9 pubmed publisher
  • immunohistochemistry; mouse
Ivaniutsin U, Chen Y, Mason J, Price D, Pratt T. Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex. Neural Dev. 2009;4:3 pubmed publisher
Kim E, Jiang M, Huang H, Zhang Y, Tjota N, Gao X, et al. Isl1 Regulation of Nkx2.1 in the Early Foregut Epithelium Is Required for Trachea-Esophageal Separation and Lung Lobation. Dev Cell. 2019;51:675-683.e4 pubmed publisher
Le Dreau G, Escalona R, Fueyo R, Herrera A, Martinez J, Usieto S, et al. E proteins sharpen neurogenesis by modulating proneural bHLH transcription factors' activity in an E-box-dependent manner. elife. 2018;7: pubmed publisher
Coulter M, Dorobantu C, Lodewijk G, Delalande F, Cianférani S, Ganesh V, et al. The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles. Cell Rep. 2018;24:973-986.e8 pubmed publisher
Okano Uchida T, Kent L, Ouseph M, McCarty B, Frank J, Kladney R, et al. Endoreduplication of the mouse genome in the absence of ORC1. Genes Dev. 2018;32:978-990 pubmed publisher
Liu W, Chen S, Li Z, Lee C, Mirzaa G, Dobyns W, et al. PARD3 dysfunction in conjunction with dynamic HIPPO signaling drives cortical enlargement with massive heterotopia. Genes Dev. 2018;32:763-780 pubmed publisher
Singh K, Lilleväli K, Gilbert S, Bregin A, Narvik J, Jayaram M, et al. The combined impact of IgLON family proteins Lsamp and Neurotrimin on developing neurons and behavioral profiles in mouse. Brain Res Bull. 2018;140:5-18 pubmed publisher
Gawrzak S, Rinaldi L, Gregorio S, Arenas E, Salvador F, Urosevic J, et al. MSK1 regulates luminal cell differentiation and metastatic dormancy in ER+ breast cancer. Nat Cell Biol. 2018;20:211-221 pubmed publisher
Haj M, Wijeweera A, Rudnizky S, Taunton J, Pnueli L, Melamed P. Mitogen- and stress-activated protein kinase 1 is required for gonadotropin-releasing hormone-mediated activation of gonadotropin α-subunit expression. J Biol Chem. 2017;292:20720-20731 pubmed publisher
Boubakar L, Falk J, Ducuing H, Thoinet K, Reynaud F, Derrington E, et al. Molecular Memory of Morphologies by Septins during Neuron Generation Allows Early Polarity Inheritance. Neuron. 2017;95:834-851.e5 pubmed publisher
Stanley Hasnain S, Hauck L, Grothe D, Aschar Sobbi R, Beca S, Butany J, et al. p53 and Mdm2 act synergistically to maintain cardiac homeostasis and mediate cardiomyocyte cell cycle arrest through a network of microRNAs. Cell Cycle. 2017;16:1585-1600 pubmed publisher
Imperadore P, Shah S, Makarenkova H, Fiorito G. Nerve degeneration and regeneration in the cephalopod mollusc Octopus vulgaris: the case of the pallial nerve. Sci Rep. 2017;7:46564 pubmed publisher
Gromova A, Voronov D, Yoshida M, Thotakura S, Meech R, Dartt D, et al. Lacrimal Gland Repair Using Progenitor Cells. Stem Cells Transl Med. 2017;6:88-98 pubmed publisher
Matheson T, Kaufman P. The p150N domain of chromatin assembly factor-1 regulates Ki-67 accumulation on the mitotic perichromosomal layer. Mol Biol Cell. 2017;28:21-29 pubmed publisher
Faire M, Skillern A, Arora R, Nguyen D, Wang J, Chamberlain C, et al. Follicle dynamics and global organization in the intact mouse ovary. Dev Biol. 2015;403:69-79 pubmed publisher
Jiang M, Ku W, Zhou Z, Dellon E, Falk G, Nakagawa H, et al. BMP-driven NRF2 activation in esophageal basal cell differentiation and eosinophilic esophagitis. J Clin Invest. 2015;125:1557-68 pubmed publisher
Handke B, Szabad J, Lidsky P, Hafen E, Lehner C. Towards long term cultivation of Drosophila wing imaginal discs in vitro. PLoS ONE. 2014;9:e107333 pubmed publisher
Liu Z, Li W, Ma X, Ding N, Spallotta F, Southon E, et al. Essential role of the zinc finger transcription factor Casz1 for mammalian cardiac morphogenesis and development. J Biol Chem. 2014;289:29801-16 pubmed publisher
Wartlick O, Jülicher F, Gonzalez Gaitan M. Growth control by a moving morphogen gradient during Drosophila eye development. Development. 2014;141:1884-93 pubmed publisher
Lane A, Giménez Abián J, Clarke D. A novel chromatin tether domain controls topoisomerase II? dynamics and mitotic chromosome formation. J Cell Biol. 2013;203:471-86 pubmed publisher
Packard A, Georgas K, Michos O, Riccio P, Cebrian C, Combes A, et al. Luminal mitosis drives epithelial cell dispersal within the branching ureteric bud. Dev Cell. 2013;27:319-30 pubmed publisher
Laird D, Altshuler Keylin S, Kissner M, Zhou X, Anderson K. Ror2 enhances polarity and directional migration of primordial germ cells. PLoS Genet. 2011;7:e1002428 pubmed publisher
Robles V, Marti M, Izpisua Belmonte J. Study of pluripotency markers in zebrafish embryos and transient embryonic stem cell cultures. Zebrafish. 2011;8:57-63 pubmed publisher
Sørensen K, Lutterodt M, Mamsen L, Byskov A, Larsen J. Proliferation of germ cells and somatic cells in first trimester human embryonic gonads as indicated by S and S+G2+M phase fractions. Cell Prolif. 2011;44:224-33 pubmed publisher
Lau P, Cheung P. Histone code pathway involving H3 S28 phosphorylation and K27 acetylation activates transcription and antagonizes polycomb silencing. Proc Natl Acad Sci U S A. 2011;108:2801-6 pubmed publisher
Stevens H, Smith K, Maragnoli M, Fagel D, Borok E, Shanabrough M, et al. Fgfr2 is required for the development of the medial prefrontal cortex and its connections with limbic circuits. J Neurosci. 2010;30:5590-602 pubmed publisher
Yin Y, White A, Huh S, Hilton M, Kanazawa H, Long F, et al. An FGF-WNT gene regulatory network controls lung mesenchyme development. Dev Biol. 2008;319:426-36 pubmed publisher
Kunath T, Saba El Leil M, Almousailleakh M, Wray J, Meloche S, Smith A. FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment. Development. 2007;134:2895-902 pubmed
Mateescu B, England P, Halgand F, Yaniv M, Muchardt C. Tethering of HP1 proteins to chromatin is relieved by phosphoacetylation of histone H3. EMBO Rep. 2004;5:490-6 pubmed
Goto H, Tomono Y, Ajiro K, Kosako H, Fujita M, Sakurai M, et al. Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation. J Biol Chem. 1999;274:25543-9 pubmed
product information
Product No. :
H9908
Product Name :
Monoclonal Anti-phospho-Histone H3 (pSer 28 ) antibody produced in rat
Host :
rat
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
HTA28
Applications/Description :
Suitable for flow cytometry; western blot: 0.5-1 μg/mL using whole extract of cultured human acute T cell leukemia Jurkat cells treated with Nocodazole.; microarray; immunocytochemistry using 3.7% formaldehyde-methanol fixation
Species Reactivity :
human, bovine, hamster, mouse
Isotype :
IgG2a
Concentration :
~0.5 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA