product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-HLA Class I Antigen antibody produced in mouse
catalog :
H1650
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
W6/32
reactivity :
human
application :
immunoprecipitation, flow cytometry
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 1a
Cardinaud S, Urrutia A, Rouers A, Coulon P, Kervevan J, Richetta C, et al. Triggering of TLR-3, -4, NOD2, and DC-SIGN reduces viral replication and increases T-cell activation capacity of HIV-infected human dendritic cells. Eur J Immunol. 2017;47:818-829 pubmed publisher
  • flow cytometry; human
  • immunoprecipitation; human
Parkinson M, Piper S, Bright N, Evans J, Boname J, Bowers K, et al. A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I. Biochem J. 2015;471:79-88 pubmed publisher
  • flow cytometry; human; fig 5
Gabaev I, Elbasani E, Ameres S, Steinbrück L, Stanton R, Döring M, et al. Expression of the human cytomegalovirus UL11 glycoprotein in viral infection and evaluation of its effect on virus-specific CD8 T cells. J Virol. 2014;88:14326-39 pubmed publisher
Du W, Tang H, Lei Z, Zhu J, Zeng Y, Liu Z, et al. miR-335-5p inhibits TGF-β1-induced epithelial-mesenchymal transition in non-small cell lung cancer via ROCK1. Respir Res. 2019;20:225 pubmed publisher
Lin S, Huang C, Sun J, Bollt O, Wang X, Martine E, et al. The mitochondrial deoxyguanosine kinase is required for cancer cell stemness in lung adenocarcinoma. EMBO Mol Med. 2019;11:e10849 pubmed publisher
Xu S, Zheng Y, Zhang L, Wang P, Niu C, Wu T, et al. Long Non-coding RNA LINC00628 Interacts Epigenetically with the LAMA3 Promoter and Contributes to Lung Adenocarcinoma. Mol Ther Nucleic Acids. 2019;18:166-182 pubmed publisher
Jiang X, Liu W, Zhu X, Xu X. Evaluation of EGFR mutations in NSCLC with highly sensitive droplet digital PCR assays. Mol Med Rep. 2019;20:593-603 pubmed publisher
Oo Z, Proctor M, Stevenson A, Nazareth D, Fernando M, Daignault S, et al. Combined use of subclinical hydroxyurea and CHK1 inhibitor effectively controls melanoma and lung cancer progression, with reduced normal tissue toxicity compared to gemcitabine. Mol Oncol. 2019;13:1503-1518 pubmed publisher
Yan H, Chen X, Li Y, Fan L, Tai Y, Zhou Y, et al. MiR-1205 functions as a tumor suppressor by disconnecting the synergy between KRAS and MDM4/E2F1 in non-small cell lung cancer. Am J Cancer Res. 2019;9:312-329 pubmed
Sun Y, Bandi M, Lofton T, Smith M, Bristow C, Carugo A, et al. Functional Genomics Reveals Synthetic Lethality between Phosphogluconate Dehydrogenase and Oxidative Phosphorylation. Cell Rep. 2019;26:469-482.e5 pubmed publisher
Yang T, Zhang W, Wang L, Xiao C, Guo B, Gong Y, et al. Long intergenic noncoding RNA-p21 inhibits apoptosis by decreasing PUMA expression in non-small cell lung cancer. J Int Med Res. 2019;47:481-493 pubmed publisher
Chen X, Zhu H, Ye W, Cui Y, Chen M. MicroRNA‑29a enhances cisplatin sensitivity in non‑small cell lung cancer through the regulation of REV3L. Mol Med Rep. 2019;19:831-840 pubmed publisher
Obrist F, Michels J, Durand S, Chery A, Pol J, Levesque S, et al. Metabolic vulnerability of cisplatin-resistant cancers. EMBO J. 2018;37: pubmed publisher
Shin M, Franks C, Hsu K. Isoform-selective activity-based profiling of ERK signaling. Chem Sci. 2018;9:2419-2431 pubmed publisher
Du W, Ni L, Liu B, Wei Y, Lv Y, Qiang S, et al. Upregulation of SALL4 by EGFR activation regulates the stemness of CD44-positive lung cancer. Oncogenesis. 2018;7:36 pubmed publisher
Jin D, Kim Y, Lee B, Han J, Kim H, Shim Y, et al. Metformin induces cell cycle arrest at the G1 phase through E2F8 suppression in lung cancer cells. Oncotarget. 2017;8:101509-101519 pubmed publisher
Topper M, Vaz M, Chiappinelli K, Destefano Shields C, Niknafs N, Yen R, et al. Epigenetic Therapy Ties MYC Depletion to Reversing Immune Evasion and Treating Lung Cancer. Cell. 2017;171:1284-1300.e21 pubmed publisher
Xiao Z, Gaertner S, Morresi Hauf A, Genzel R, Duell T, Ullrich A, et al. Metformin Triggers Autophagy to Attenuate Drug-Induced Apoptosis in NSCLC Cells, with Minor Effects on Tumors of Diabetic Patients. Neoplasia. 2017;19:385-395 pubmed publisher
Miles A, Burr S, Grice G, Nathan J. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels. elife. 2017;6: pubmed publisher
Ioris R, Galié M, Ramadori G, Anderson J, Charollais A, Konstantinidou G, et al. SIRT6 Suppresses Cancer Stem-like Capacity in Tumors with PI3K Activation Independently of Its Deacetylase Activity. Cell Rep. 2017;18:1858-1868 pubmed publisher
Wang Y, Liu H, Diao L, Potter A, Zhang J, Qiao Y, et al. Hsp90 Inhibitor Ganetespib Sensitizes Non-Small Cell Lung Cancer to Radiation but Has Variable Effects with Chemoradiation. Clin Cancer Res. 2016;22:5876-5886 pubmed
Wan J, Wu W, Huang Y, Ge W, Liu S. Incomplete radiofrequency ablation accelerates proliferation and angiogenesis of residual lung carcinomas via HSP70/HIF-1?. Oncol Rep. 2016;36:659-68 pubmed publisher
Pillai S, Nguyen J, Johnson J, Haura E, Coppola D, Chellappan S. Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis. Nat Commun. 2015;6:10072 pubmed publisher
de Wit S, van Dalum G, Lenferink A, Tibbe A, Hiltermann T, Groen H, et al. The detection of EpCAM(+) and EpCAM(-) circulating tumor cells. Sci Rep. 2015;5:12270 pubmed publisher
van der Mijn J, Sol N, Mellema W, Jimenez C, Piersma S, Dekker H, et al. Analysis of AKT and ERK1/2 protein kinases in extracellular vesicles isolated from blood of patients with cancer. J Extracell Vesicles. 2014;3:25657 pubmed publisher
Nitta T, Mitsuhashi T, Hatanaka Y, Miyamoto M, Oba K, Tsuchikawa T, et al. Prognostic significance of epithelial-mesenchymal transition-related markers in extrahepatic cholangiocarcinoma: comprehensive immunohistochemical study using a tissue microarray. Br J Cancer. 2014;111:1363-72 pubmed publisher
Nair S, Bora Singhal N, Perumal D, Chellappan S. Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner. Mol Cancer. 2014;13:173 pubmed publisher
Patel A, Chougule M, Singh M. EphA2 targeting pegylated nanocarrier drug delivery system for treatment of lung cancer. Pharm Res. 2014;31:2796-809 pubmed publisher
Nielsen N, Pascal V, Fasth A, Sundström Y, Galsgaard E, Ahern D, et al. Balance between activating NKG2D, DNAM-1, NKp44 and NKp46 and inhibitory CD94/NKG2A receptors determine natural killer degranulation towards rheumatoid arthritis synovial fibroblasts. Immunology. 2014;142:581-93 pubmed publisher
Shi X, Sun M, Liu H, Yao Y, Kong R, Chen F, et al. A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer. Mol Carcinog. 2015;54 Suppl 1:E1-E12 pubmed publisher
Kani K, Faca V, Hughes L, Zhang W, Fang Q, Shahbaba B, et al. Quantitative proteomic profiling identifies protein correlates to EGFR kinase inhibition. Mol Cancer Ther. 2012;11:1071-81 pubmed publisher
Bugyik E, Dezso K, Reiniger L, László V, Tovari J, Timar J, et al. Lack of angiogenesis in experimental brain metastases. J Neuropathol Exp Neurol. 2011;70:979-91 pubmed publisher
Watanabe K, Emoto N, Hamano E, Sunohara M, Kawakami M, Kage H, et al. Genome structure-based screening identified epigenetically silenced microRNA associated with invasiveness in non-small-cell lung cancer. Int J Cancer. 2012;130:2580-90 pubmed publisher
Chiu H, Chou D, Huang C, Lin W, Lien T, Yen K, et al. Suppression of Stat3 activity sensitizes gefitinib-resistant non small cell lung cancer cells. Biochem Pharmacol. 2011;81:1263-70 pubmed publisher
Hao W, Chang C, Tsao C, Xu J. Oligomycin-induced bioenergetic adaptation in cancer cells with heterogeneous bioenergetic organization. J Biol Chem. 2010;285:12647-54 pubmed publisher
Alzhanova D, Edwards D, Hammarlund E, Scholz I, Horst D, Wagner M, et al. Cowpox virus inhibits the transporter associated with antigen processing to evade T cell recognition. Cell Host Microbe. 2009;6:433-45 pubmed publisher
Zhong D, Liu X, Khuri F, Sun S, Vertino P, Zhou W. LKB1 is necessary for Akt-mediated phosphorylation of proapoptotic proteins. Cancer Res. 2008;68:7270-7 pubmed publisher
Clements C, Kjer Nielsen L, Kostenko L, McCluskey J, Rossjohn J. The production, purification and crystallization of a soluble form of the nonclassical MHC HLA-G: the essential role of cobalt. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:70-3 pubmed
Wary K, Humtsoe J. Anti-lipid phosphate phosphohydrolase-3 (LPP3) antibody inhibits bFGF- and VEGF-induced capillary morphogenesis of endothelial cells. Cell Commun Signal. 2005;3:9 pubmed
Minetti C, Gattorno M, Repetto S, Gregorio A, Pedemonte M, Assereto S, et al. Chemokine receptor CCR7 is expressed in muscle fibers in juvenile dermatomyositis. Biochem Biophys Res Commun. 2005;333:540-3 pubmed
Akatsuka Y, Nishida T, Kondo E, Miyazaki M, Taji H, Iida H, et al. Identification of a polymorphic gene, BCL2A1, encoding two novel hematopoietic lineage-specific minor histocompatibility antigens. J Exp Med. 2003;197:1489-500 pubmed
Johnson J, Nicot C, Fullen J, Ciminale V, Casareto L, Mulloy J, et al. Free major histocompatibility complex class I heavy chain is preferentially targeted for degradation by human T-cell leukemia/lymphotropic virus type 1 p12(I) protein. J Virol. 2001;75:6086-94 pubmed
Pique C, Ureta Vidal A, Gessain A, Chancerel B, Gout O, Tamouza R, et al. Evidence for the chronic in vivo production of human T cell leukemia virus type I Rof and Tof proteins from cytotoxic T lymphocytes directed against viral peptides. J Exp Med. 2000;191:567-72 pubmed
product information
Product No. :
H1650
Product Name :
Monoclonal Anti-HLA Class I Antigen antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
W6/32
Applications/Description :
Suitable for flow cytometry: 5 μL using 1 × 10 6 cells; indirect immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections.; immunoprecipitation (IP)
Species Reactivity :
human, monkey
Isotype :
IgG2a
Storage :
Store at 2-8°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA