product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Anti-GSDMD (126-138) antibody produced in rabbit
catalog :
G7422
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, western blot knockout validation
more info or order :
citations: 10
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:2000; fig s8
Dick M, Sborgi L, Rühl S, Hiller S, Broz P. ASC filament formation serves as a signal amplification mechanism for inflammasomes. Nat Commun. 2016;7:11929 pubmed publisher
  • western blot; human; 1:1000; fig 7
Eichholz K, Bru T, Tran T, Fernandes P, Welles H, Mennechet F, et al. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog. 2016;12:e1005871 pubmed publisher
Wang Y, Shi P, Chen Q, Huang Z, Zou D, Zhang J, et al. Mitochondrial ROS promote macrophage pyroptosis by inducing GSDMD oxidation. J Mol Cell Biol. 2019;: pubmed publisher
Platnich J, Chung H, Lau A, Sandall C, Bondzi Simpson A, Chen H, et al. Shiga Toxin/Lipopolysaccharide Activates Caspase-4 and Gasdermin D to Trigger Mitochondrial Reactive Oxygen Species Upstream of the NLRP3 Inflammasome. Cell Rep. 2018;25:1525-1536.e7 pubmed publisher
Hara H, Seregin S, Yang D, Fukase K, Chamaillard M, Alnemri E, et al. The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection. Cell. 2018;175:1651-1664.e14 pubmed publisher
Zhou B, Zhang J, Liu X, Chen H, Ai Y, Cheng K, et al. Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis. Cell Res. 2018;28:1171-1185 pubmed publisher
Mandal P, Feng Y, Lyons J, Berger S, Otani S, DeLaney A, et al. Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock. Immunity. 2018;49:42-55.e6 pubmed publisher
Chu L, Indramohan M, Ratsimandresy R, Gangopadhyay A, Morris E, Monack D, et al. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat Commun. 2018;9:996 pubmed publisher
Khanova E, Wu R, Wang W, Yan R, Chen Y, French S, et al. Pyroptosis by caspase11/4-gasdermin-D pathway in alcoholic hepatitis in mice and patients. Hepatology. 2018;67:1737-1753 pubmed publisher
Lei X, Zhang Z, Xiao X, Qi J, He B, Wang J. Enterovirus 71 Inhibits Pyroptosis through Cleavage of Gasdermin D. J Virol. 2017;91: pubmed publisher
product information
Product No. :
G7422
Product Name :
Anti-GSDMD (126-138) antibody produced in rabbit
Host :
rabbit
Conjugate :
unconjugated
Antibody Type :
polyclonal
Applications/Description :
Suitable for immunoblotting: 1:250-1:500 using human HEK-293T cells over-expressing human recombinant. GSDMDC1.
Species Reactivity :
human
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA