product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody produced in mouse
catalog :
G5419
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
K-87
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section
more info or order :
citations: 24
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 3h
Li S, Zang Z, He J, Chen X, Yu S, Pei Y, et al. Expression of pannexin 1 and 2 in cortical lesions from intractable epilepsy patients with focal cortical dysplasia. Oncotarget. 2017;8:6883-6895 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:1000; fig 1
Ramírez Franco J, Munoz Cuevas F, Lujan R, Jurado S. Excitatory and Inhibitory Neurons in the Hippocampus Exhibit Molecularly Distinct Large Dense Core Vesicles. Front Cell Neurosci. 2016;10:202 pubmed publisher
  • immunohistochemistry; mouse; 1:20,000; loading ...; tbl 1
Fujimoto H, Konno K, Watanabe M, Jinno S. Late postnatal shifts of parvalbumin and nitric oxide synthase expression within the GABAergic and glutamatergic phenotypes of inferior colliculus neurons. J Comp Neurol. 2017;525:868-884 pubmed publisher
Huang T, Hsu T, Lin M, Chuang H, Hu H, Sun C, et al. Interhemispheric Connectivity Potentiates the Basolateral Amygdalae and Regulates Social Interaction and Memory. Cell Rep. 2019;29:34-48.e4 pubmed publisher
Tsuboi M, Kondo K, Masuda K, Tange S, Kajiura K, Kohmoto T, et al. Prognostic significance of GAD1 overexpression in patients with resected lung adenocarcinoma. Cancer Med. 2019;8:4189-4199 pubmed publisher
Gunn B, Sanchez G, Lynch G, Baram T, Chen Y. Hyper-diversity of CRH interneurons in mouse hippocampus. Brain Struct Funct. 2019;224:583-598 pubmed publisher
Yamamoto Y, Kida H, Kagawa Y, Yasumoto Y, Miyazaki H, Islam A, et al. FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase67 Promoter Region. J Neurosci. 2018;38:10411-10423 pubmed publisher
Fukushima N, Nagao T. Exposure to bisphenol A affects GABAergic neuron differentiation in neurosphere cultures. Neuroreport. 2018;29:712-717 pubmed publisher
Ding Y, Zheng Y, Liu T, Chen T, Wang C, Sun Q, et al. Changes in GABAergic markers accompany degradation of neuronal function in the primary visual cortex of senescent rats. Sci Rep. 2017;7:14897 pubmed publisher
Wohleb E, Wu M, Gerhard D, Taylor S, Picciotto M, Alreja M, et al. GABA interneurons mediate the rapid antidepressant-like effects of scopolamine. J Clin Invest. 2016;126:2482-94 pubmed publisher
Ji B, Higa K, Kim M, Zhou L, Young J, Geyer M, et al. Inhibition of protein translation by the DISC1-Boymaw fusion gene from a Scottish family with major psychiatric disorders. Hum Mol Genet. 2014;23:5683-705 pubmed publisher
Liang C, Quang Nguyen T, Marks G. Inhibitory and excitatory amino acid neurotransmitters are utilized by the projection from the dorsal deep mesencephalic nucleus to the sublaterodorsal nucleus REM sleep induction zone. Brain Res. 2014;1567:1-12 pubmed publisher
Buret L, van den Buuse M. Corticosterone treatment during adolescence induces down-regulation of reelin and NMDA receptor subunit GLUN2C expression only in male mice: implications for schizophrenia. Int J Neuropsychopharmacol. 2014;17:1221-32 pubmed publisher
Reish N, Maltare A, McKeown A, Laszczyk A, Kraft T, Gross A, et al. The age-regulating protein klotho is vital to sustain retinal function. Invest Ophthalmol Vis Sci. 2013;54:6675-85 pubmed publisher
Ji B, Wang X, Pinto Duarte A, Kim M, Caldwell S, Young J, et al. Prolonged Ketamine Effects in Sp4 Hypomorphic Mice: Mimicking Phenotypes of Schizophrenia. PLoS ONE. 2013;8:e66327 pubmed publisher
Kim M, Soontornniyomkij V, Ji B, ZHOU X. System-wide immunohistochemical analysis of protein co-localization. PLoS ONE. 2012;7:e32043 pubmed publisher
Rossi S, Muzio L, De Chiara V, Grasselli G, Musella A, Musumeci G, et al. Impaired striatal GABA transmission in experimental autoimmune encephalomyelitis. Brain Behav Immun. 2011;25:947-56 pubmed publisher
Chen Y, Dong E, Grayson D. Analysis of the GAD1 promoter: trans-acting factors and DNA methylation converge on the 5' untranslated region. Neuropharmacology. 2011;60:1075-87 pubmed publisher
Chan O, Cheng H, HERZOG R, Czyzyk D, Zhu W, Wang A, et al. Increased GABAergic tone in the ventromedial hypothalamus contributes to suppression of counterregulatory responses after antecedent hypoglycemia. Diabetes. 2008;57:1363-70 pubmed publisher
Wei J, Wu J. Post-translational regulation of L-glutamic acid decarboxylase in the brain. Neurochem Res. 2008;33:1459-65 pubmed publisher
Akbarian S, Huang H. Molecular and cellular mechanisms of altered GAD1/GAD67 expression in schizophrenia and related disorders. Brain Res Rev. 2006;52:293-304 pubmed
Guidotti A, Auta J, Davis J, Di Giorgi Gerevini V, Dwivedi Y, Grayson D, et al. Decrease in reelin and glutamic acid decarboxylase67 (GAD67) expression in schizophrenia and bipolar disorder: a postmortem brain study. Arch Gen Psychiatry. 2000;57:1061-9 pubmed
Yamashita K, Cram D, Harrison L. Molecular cloning of full-length glutamic acid decarboxylase 67 from human pancreas and islets. Biochem Biophys Res Commun. 1993;192:1347-52 pubmed
Kaufman D, Houser C, Tobin A. Two forms of the gamma-aminobutyric acid synthetic enzyme glutamate decarboxylase have distinct intraneuronal distributions and cofactor interactions. J Neurochem. 1991;56:720-3 pubmed
product information
Product No. :
G5419
Product Name :
Monoclonal Anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
K-87
Applications/Description :
Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:20,000 (with antigen-retrieval step); western blot: 1:500-1:20,000 using mouse brain extract
Species Reactivity :
mouse, rat, human
Isotype :
IgG1κ
Concentration :
~1 mg/mL
Storage :
Store at -70°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA