product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Anti-Glutamine Synthetase antibody produced in rabbit
catalog :
G2781
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; loading ...; fig 2j
Bott A, Shen J, Tonelli C, Zhan L, Sivaram N, Jiang Y, et al. Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism. Cell Rep. 2019;29:1287-1298.e6 pubmed publisher
  • western blot knockout validation; mouse; 1:1500; loading ...; fig 8b, s6a, 6
Russell J, Lu W, Okabe H, Abrams M, Oertel M, Poddar M, et al. Hepatocyte-Specific β-Catenin Deletion During Severe Liver Injury Provokes Cholangiocytes to Differentiate Into Hepatocytes. Hepatology. 2019;69:742-759 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 3e
Dragan M, Nguyen M, Guzman S, Goertzen C, Brackstone M, Dhillo W, et al. G protein-coupled kisspeptin receptor induces metabolic reprograming and tumorigenesis in estrogen receptor-negative breast cancer. Cell Death Dis. 2020;11:106 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:5000; loading ...; fig 2a
Nomura Komoike K, Saitoh F, Fujieda H. Phosphatidylserine recognition and Rac1 activation are required for Müller glia proliferation, gliosis and phagocytosis after retinal injury. Sci Rep. 2020;10:1488 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3b
Zhang X, Olsavszky V, Yin Y, Wang B, Engleitner T, Ollinger R, et al. Angiocrine Hepatocyte Growth Factor Signaling Controls Physiological Organ and Body Size and Dynamic Hepatocyte Proliferation to Prevent Liver Damage during Regeneration. Am J Pathol. 2020;190:358-371 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s1e
Wang B, Kaufmann B, Engleitner T, Lu M, Mogler C, Olsavszky V, et al. Brg1 promotes liver regeneration after partial hepatectomy via regulation of cell cycle. Sci Rep. 2019;9:2320 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s2b
Akiel M, Guo C, Li X, Rajasekaran D, Mendoza R, Robertson C, et al. IGFBP7 Deletion Promotes Hepatocellular Carcinoma. Cancer Res. 2017;77:4014-4025 pubmed publisher
  • western blot; human; 1:1000; fig st1
Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 2
Skytt D, Toft Kehler A, Brændstrup C, Cejvanovic S, Gurubaran I, Bergersen L, et al. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells. Biomed Res Int. 2016;2016:1087647 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:300; loading ...; fig 1d
Sanges D, Simonte G, Di Vicino U, Romo N, Pinilla I, Nicolas M, et al. Reprogramming Müller glia via in vivo cell fusion regenerates murine photoreceptors. J Clin Invest. 2016;126:3104-16 pubmed publisher
Bonilla Pons S, Nakagawa S, Bahima E, Fernández Blanco Á, Pesaresi M, D Antin J, et al. Müller glia fused with adult stem cells undergo neural differentiation in human retinal models. EBioMedicine. 2022;77:103914 pubmed publisher
Korotkov A, Broekaart D, Banchaewa L, Pustjens B, van Scheppingen J, Anink J, et al. microRNA-132 is overexpressed in glia in temporal lobe epilepsy and reduces the expression of pro-epileptogenic factors in human cultured astrocytes. Glia. 2020;68:60-75 pubmed publisher
Huyghe D, Denninger A, Voss C, Frank P, Gao N, Brandon N, et al. Phosphorylation of Glutamine Synthetase on Threonine 301 Contributes to Its Inactivation During Epilepsy. Front Mol Neurosci. 2019;12:120 pubmed publisher
Molina L, Yang H, Adebayo Michael A, Oertel M, Bell A, Singh S, et al. mTOR inhibition affects Yap1-β-catenin-induced hepatoblastoma growth and development. Oncotarget. 2019;10:1475-1490 pubmed publisher
Neckles V, Morton M, Holmberg J, Sokolov A, Nottoli T, Liu D, et al. A transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles. Sci Rep. 2019;9:3094 pubmed publisher
Zhang H, Zou Y, Lei H. Regional metabolic differences in rat prefrontal cortex measured with in vivo 1 H-MRS correlate with regional histochemical differences. NMR Biomed. 2019;32:e4024 pubmed publisher
Boccella S, Guida F, De Logu F, De Gregorio D, Mazzitelli M, Belardo C, et al. Ketones and pain: unexplored role of hydroxyl carboxylic acid receptor type 2 in the pathophysiology of neuropathic pain. FASEB J. 2019;33:1062-1073 pubmed publisher
Braeuning A, Kollotzek F, Zeller E, Knorpp T, Templin M, Schwarz M. Mouse Hepatomas with Ha-ras and B-raf Mutations Differ in Mitogen-Activated Protein Kinase Signaling and Response to Constitutive Androstane Receptor Activation. Drug Metab Dispos. 2018;46:1462-1465 pubmed publisher
Tsagianni A, Mars W, Bhushan B, Bowen W, Orr A, Stoops J, et al. Combined Systemic Disruption of MET and Epidermal Growth Factor Receptor Signaling Causes Liver Failure in Normal Mice. Am J Pathol. 2018;188:2223-2235 pubmed publisher
Molina L, Bell D, Tao J, Preziosi M, Pradhan Sundd T, Singh S, et al. Hepatocyte-Derived Lipocalin 2 Is a Potential Serum Biomarker Reflecting Tumor Burden in Hepatoblastoma. Am J Pathol. 2018;188:1895-1909 pubmed publisher
Augustine J, Pavlou S, O Hare M, Harkin K, Stitt A, Curtis T, et al. Characterization of a Spontaneously Immortalized Murine Müller Glial Cell Line QMMuC-1. Invest Ophthalmol Vis Sci. 2018;59:1666-1674 pubmed publisher
Hu Q, Ottestad Hansen S, Holmseth S, Hassel B, Danbolt N, Zhou Y. Expression of Glutamate Transporters in Mouse Liver, Kidney, and Intestine. J Histochem Cytochem. 2018;66:189-202 pubmed publisher
Moon K, Kim H, Lee D, Rao M, Levine E, Lim D, et al. Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development. Dev Cell. 2018;44:13-28.e3 pubmed publisher
Chen J, He W, Hu X, Shen Y, Cao J, Wei Z, et al. A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov. 2017;3:17044 pubmed publisher
Capitanio D, Moriggi M, De Palma S, Bizzotto D, Molon S, Torretta E, et al. Collagen VI Null Mice as a Model for Early Onset Muscle Decline in Aging. Front Mol Neurosci. 2017;10:337 pubmed publisher
Ruiz M, Bégou M, Launay N, Ranea Robles P, Bianchi P, López Erauskin J, et al. OXIDATIVE STRESS AND MITOCHONDRIAL DYNAMICS MALFUNCTION ARE LINKED IN PELIZAEUS-MERZBACHER DISEASE. Brain Pathol. 2017;: pubmed publisher
Gu D, Wang S, Zhang S, Zhang P, Zhou G. Directed transdifferentiation of Müller glial cells to photoreceptors using the sonic hedgehog signaling pathway agonist purmorphamine. Mol Med Rep. 2017;16:7993-8002 pubmed publisher
Zhu Y, Li T, Ramos da Silva S, Lee J, Lu C, Eoh H, et al. A Critical Role of Glutamine and Asparagine γ-Nitrogen in Nucleotide Biosynthesis in Cancer Cells Hijacked by an Oncogenic Virus. MBio. 2017;8: pubmed publisher
Moshiri A, Humpal D, Leonard B, Imai D, Tham A, Bower L, et al. Arap1 Deficiency Causes Photoreceptor Degeneration in Mice. Invest Ophthalmol Vis Sci. 2017;58:1709-1718 pubmed publisher
Johnson Chacko L, Pechriggl E, Fritsch H, Rask Andersen H, Blumer M, Schrott Fischer A, et al. Neurosensory Differentiation and Innervation Patterning in the Human Fetal Vestibular End Organs between the Gestational Weeks 8-12. Front Neuroanat. 2016;10:111 pubmed
Shaw P, Fang J, Sang A, Wang Y, Kapiloff M, Goldberg J. Soluble Adenylyl Cyclase Is Required for Retinal Ganglion Cell and Photoreceptor Differentiation. Invest Ophthalmol Vis Sci. 2016;57:5083-5092 pubmed publisher
Soontornniyomkij V, Kesby J, Soontornniyomkij B, Kim J, Kisseleva T, Achim C, et al. Age and High-Fat Diet Effects on Glutamine Synthetase Immunoreactivity in Liver and Hippocampus and Recognition Memory in Mice. Curr Aging Sci. 2016;9:301-309 pubmed
Del Debbio C, Mir Q, Parameswaran S, Mathews S, Xia X, Zheng L, et al. Notch Signaling Activates Stem Cell Properties of Müller Glia through Transcriptional Regulation and Skp2-mediated Degradation of p27Kip1. PLoS ONE. 2016;11:e0152025 pubmed publisher
Rozas P, Lazcano P, Piña R, Cho A, Terse A, Pertusa M, et al. Targeted overexpression of tumor necrosis factor-α increases cyclin-dependent kinase 5 activity and TRPV1-dependent Ca2+ influx in trigeminal neurons. Pain. 2016;157:1346-62 pubmed publisher
da Silva L, Poulsen J, Arendt Nielsen L, Gazerani P. Botulinum neurotoxin type A modulates vesicular release of glutamate from satellite glial cells. J Cell Mol Med. 2015;19:1900-9 pubmed publisher
product information
Product No. :
G2781
Product Name :
Anti-Glutamine Synthetase antibody produced in rabbit
Host :
rabbit
Conjugate :
unconjugated
Antibody Type :
polyclonal
Applications/Description :
Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections; western blot: 1:10,000 using rat brain cytosolic fraction; microarray
Species Reactivity :
rat
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA