product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
catalog :
F3165
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M2
reactivity :
African green monkey, Saccharomycetales, Agrobacterium tumefaciens, fission yeast, human, mouse, rat, dogs, chicken, brewer's yeast, fruit fly , Xenopus laevis, thale cress, roundworm , E. coli
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, ChIP-Seq, proximity ligation assay, cross-linking immunoprecipitation, western blot knockout validation
more info or order :
citations: 5129
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; fig 2
Persaud S, Park S, Ishigami Yuasa M, Koyano Nakagawa N, Kagechika H, Wei L. All trans-retinoic acid analogs promote cancer cell apoptosis through non-genomic Crabp1 mediating ERK1/2 phosphorylation. Sci Rep. 2016;6:22396 pubmed publisher
  • western blot; human; loading ...; fig 4a
Katoh I, Maehata Y, Moriishi K, Hata R, Kurata S. C-terminal α Domain of p63 Binds to p300 to Coactivate β-Catenin. Neoplasia. 2019;21:494-503 pubmed publisher
  • western blot; brewer's yeast; 1:4000; fig 2e
van Welsem T, Korthout T, Ekkebus R, Morais D, Molenaar T, van Harten K, et al. Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism. Nucleic Acids Res. 2018;46:11251-11261 pubmed publisher
  • western blot; human; loading ...; fig 1a
Turkington H, Juozapaitis M, Tsolakos N, Corrales Aguilar E, Schwemmle M, Hale B. Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins. J Virol. 2018;92: pubmed publisher
  • western blot; mouse; fig 3f
Lamonica J, Kwon D, Goffin D, Fenik P, Johnson B, Cui Y, et al. Elevating expression of MeCP2 T158M rescues DNA binding and Rett syndrome-like phenotypes. J Clin Invest. 2017;127:1889-1904 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 1b
Wheaton K, Sarkari F, Stanly Johns B, Davarinejad H, Egorova O, Kaustov L, et al. UbE2E1/UBCH6 Is a Critical in Vivo E2 for the PRC1-catalyzed Ubiquitination of H2A at Lys-119. J Biol Chem. 2017;292:2893-2902 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 1d
Liang J, Cao R, Zhang Y, Xia Y, Zheng Y, Li X, et al. PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. Nat Commun. 2016;7:12431 pubmed publisher
  • immunohistochemistry; mouse; 1:500
Hayashi S, Akiyama R, Wong J, Tahara N, Kawakami H, Kawakami Y. Gata6-Dependent GLI3 Repressor Function is Essential in Anterior Limb Progenitor Cells for Proper Limb Development. PLoS Genet. 2016;12:e1006138 pubmed publisher
  • immunoprecipitation; human; fig 4
Glenewinkel F, Cohen M, King C, Kaspar S, Bamberg Lemper S, Mymryk J, et al. The adaptor protein DCAF7 mediates the interaction of the adenovirus E1A oncoprotein with the protein kinases DYRK1A and HIPK2. Sci Rep. 2016;6:28241 pubmed publisher
  • western blot; human; fig 1
Wang Y, Li Y, Hu G, Huang X, Rao H, Xiong X, et al. Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism. Cell Signal. 2016;28:1304-13 pubmed publisher
  • immunoprecipitation; human; 1:1000; fig 2
  • western blot; human; 1:1000; fig 2
Eichner R, Heider M, Fernández Sáiz V, van Bebber F, Garz A, Lemeer S, et al. Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity. Nat Med. 2016;22:735-43 pubmed publisher
  • western blot; thale cress; 1:1000
Li K, Yu R, Fan L, Wei N, Chen H, Deng X. DELLA-mediated PIF degradation contributes to coordination of light and gibberellin signalling in Arabidopsis. Nat Commun. 2016;7:11868 pubmed publisher
  • western blot; Agrobacterium tumefaciens; 1:2000
Jing M, Guo B, Li H, Yang B, Wang H, Kong G, et al. A Phytophthora sojae effector suppresses endoplasmic reticulum stress-mediated immunity by stabilizing plant Binding immunoglobulin Proteins. Nat Commun. 2016;7:11685 pubmed publisher
  • western blot; human; 1:5000; fig 5
Speer S, Li Z, Buta S, Payelle Brogard B, Qian L, Vigant F, et al. ISG15 deficiency and increased viral resistance in humans but not mice. Nat Commun. 2016;7:11496 pubmed publisher
  • western blot; human; 1:1000; fig 2
Aizawa S, Okamoto T, Sugiyama Y, Kouwaki T, Ito A, Suzuki T, et al. TRC8-dependent degradation of hepatitis C virus immature core protein regulates viral propagation and pathogenesis. Nat Commun. 2016;7:11379 pubmed publisher
  • immunoprecipitation; human; 1:5000; fig 4
Gandin V, Masvidal L, Cargnello M, Gyenis L, McLaughlan S, Cai Y, et al. mTORC1 and CK2 coordinate ternary and eIF4F complex assembly. Nat Commun. 2016;7:11127 pubmed publisher
  • western blot; human; 1:5000; fig 2
Douanne T, Gavard J, Bidère N. The paracaspase MALT1 cleaves the LUBAC subunit HOIL1 during antigen receptor signaling. J Cell Sci. 2016;129:1775-80 pubmed publisher
  • immunocytochemistry; human; 1:2000; fig 7
  • western blot; human; 1:2000; fig 2
Chavali P, Chandrasekaran G, Barr A, Tátrai P, Taylor C, Papachristou E, et al. A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer. Nat Commun. 2016;7:11005 pubmed publisher
  • immunoprecipitation; human; fig s2e
  • immunocytochemistry; human; fig s2c
  • western blot; human; fig s2a
Oswald F, Rodriguez P, Giaimo B, Antonello Z, Mira L, Mittler G, et al. A phospho-dependent mechanism involving NCoR and KMT2D controls a permissive chromatin state at Notch target genes. Nucleic Acids Res. 2016;44:4703-20 pubmed publisher
  • western blot; human; fig 3
Liu L, Liu X, Ren X, Tian Y, Chen Z, Xu X, et al. Smad2 and Smad3 have differential sensitivity in relaying TGFβ signaling and inversely regulate early lineage specification. Sci Rep. 2016;6:21602 pubmed publisher
  • western blot; human; fig 5
Castaño J, Morera C, Sesé B, Boue S, Bonet Costa C, Marti M, et al. SETD7 Regulates the Differentiation of Human Embryonic Stem Cells. PLoS ONE. 2016;11:e0149502 pubmed publisher
  • western blot; human; fig 1
Baietti M, Simíček M, Abbasi Asbagh L, Radaelli E, Lievens S, Crowther J, et al. OTUB1 triggers lung cancer development by inhibiting RAS monoubiquitination. EMBO Mol Med. 2016;8:288-303 pubmed publisher
  • western blot; human; fig 5
Wike C, Graves H, Hawkins R, Gibson M, Ferdinand M, Zhang T, et al. Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis. elife. 2016;5:e11402 pubmed publisher
  • western blot; human; fig 1
Kim J, He X, Orr B, Wutz G, Hill V, Peters J, et al. Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2. PLoS Genet. 2016;12:e1005865 pubmed publisher
  • cross-linking immunoprecipitation; human; fig s1e
  • western blot; human; fig 3a
Chen S, Blank M, Iyer A, Huang B, Wang L, Grummt I, et al. SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing. Nat Commun. 2016;7:10734 pubmed publisher
  • western blot; mouse; 1:2000; fig 4
White Y, Bagchi A, Van Ziffle J, Inguva A, Bollag G, Zhang C, et al. KRAS insertion mutations are oncogenic and exhibit distinct functional properties. Nat Commun. 2016;7:10647 pubmed publisher
  • western blot; brewer's yeast; fig s4
Brown M, Kim Y, Williams G, Huck J, Surtees J, Finkelstein I. Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesions. Nat Commun. 2016;7:10607 pubmed publisher
  • western blot; human; fig 5
Podmirseg S, Jäkel H, Ranches G, Kullmann M, Sohm B, Villunger A, et al. Caspases uncouple p27(Kip1) from cell cycle regulated degradation and abolish its ability to stimulate cell migration and invasion. Oncogene. 2016;35:4580-90 pubmed publisher
  • immunoprecipitation; mouse
  • western blot; mouse
de Dieuleveult M, Yen K, Hmitou I, Depaux A, Boussouar F, Bou Dargham D, et al. Genome-wide nucleosome specificity and function of chromatin remodellers in ES cells. Nature. 2016;530:113-6 pubmed publisher
  • immunocytochemistry; human; fig 5
Couderc C, Boin A, Fuhrmann L, Vincent Salomon A, Mandati V, Kieffer Y, et al. AMOTL1 Promotes Breast Cancer Progression and Is Antagonized by Merlin. Neoplasia. 2016;18:10-24 pubmed publisher
  • immunoprecipitation; human; fig 5e
  • western blot; human; 1:1000; fig 5e
Wang Z, Huang M, Ma X, Li H, Tang T, Guo C. REV1 promotes PCNA monoubiquitylation through interacting with ubiquitylated RAD18. J Cell Sci. 2016;129:1223-33 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • immunocytochemistry; mouse; 1:250
Luchsinger L, de Almeida M, Corrigan D, Mumau M, Snoeck H. Mitofusin 2 maintains haematopoietic stem cells with extensive lymphoid potential. Nature. 2016;529:528-31 pubmed publisher
  • western blot; human; fig s1
Lechtenberg B, Rajput A, Sanishvili R, Dobaczewska M, Ware C, Mace P, et al. Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase mechanism and regulation. Nature. 2016;529:546-50 pubmed publisher
  • western blot; human; fig 4d
Chavoshi S, Egorova O, Lacdao I, Farhadi S, Sheng Y, Saridakis V. Identification of Kaposi Sarcoma Herpesvirus (KSHV) vIRF1 Protein as a Novel Interaction Partner of Human Deubiquitinase USP7. J Biol Chem. 2016;291:6281-91 pubmed publisher
  • western blot; human; 1:2000; fig 1
Soo Lee N, Jin Chung H, Kim H, Yun Lee S, Ji J, Seo Y, et al. TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage. Nat Commun. 2016;7:10463 pubmed publisher
  • western blot; human; fig 1
Du Y, Yamaguchi H, Wei Y, Hsu J, Wang H, Hsu Y, et al. Blocking c-Met-mediated PARP1 phosphorylation enhances anti-tumor effects of PARP inhibitors. Nat Med. 2016;22:194-201 pubmed publisher
  • western blot; mouse; fig s4b
Kibe T, Zimmermann M, de Lange T. TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres. Mol Cell. 2016;61:236-46 pubmed publisher
  • immunocytochemistry; human; 1 ug/ml; fig 3
  • western blot; human; 1 ug/ml; fig 3
Singh H, Li M, Hall L, Chen S, Sukur S, Lu R, et al. MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain. Neuroscience. 2016;317:76-107 pubmed publisher
  • western blot; human; fig 3
Kuo C, Li X, Stark J, Shih H, Ann D. RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner. Cell Cycle. 2016;15:787-98 pubmed publisher
  • immunoprecipitation; human; fig 3
  • western blot; human; fig 3
Zhang Q, Wan M, Shi J, Horita D, Miller L, Kute T, et al. Yin Yang 1 promotes mTORC2-mediated AKT phosphorylation. J Mol Cell Biol. 2016;8:232-43 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 2
Yamamoto H, Ramos Molina B, Lick A, Prideaux M, Albornoz V, Bonewald L, et al. Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transition. Bone. 2016;84:120-130 pubmed publisher
  • western blot; human; 1:1000; fig 4
Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
  • western blot; human; fig 1
Wang M, Itoh M, Li S, Hida Y, Ohta K, Hayakawa M, et al. CED-4 is an mRNA-binding protein that delivers ced-3 mRNA to ribosomes. Biochem Biophys Res Commun. 2016;470:48-53 pubmed publisher
  • ChIP-Seq; mouse; tbl  s3
Yasuma K, Yasunaga J, Takemoto K, Sugata K, Mitobe Y, Takenouchi N, et al. HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT). PLoS Pathog. 2016;12:e1005372 pubmed publisher
  • western blot; human; 1:1000; fig 3
Wilhelm K, Happel K, Eelen G, Schoors S, Oellerich M, Lim R, et al. FOXO1 couples metabolic activity and growth state in the vascular endothelium. Nature. 2016;529:216-20 pubmed publisher
  • western blot; thale cress; fig 3b
Huang P, Chan P, Su P, Chen L, Li H. Chloroplast Hsp93 Directly Binds to Transit Peptides at an Early Stage of the Preprotein Import Process. Plant Physiol. 2016;170:857-66 pubmed publisher
  • western blot; human; fig 6
Lagarrigue S, Lopez Mejia I, Denechaud P, Escoté X, Castillo Armengol J, Jimenez V, et al. CDK4 is an essential insulin effector in adipocytes. J Clin Invest. 2016;126:335-48 pubmed publisher
  • immunocytochemistry; human; 1:2000; fig 4
  • western blot; human; 1:10,000; fig 2
Kim J, Lee K, Rhee K. PLK1 regulation of PCNT cleavage ensures fidelity of centriole separation during mitotic exit. Nat Commun. 2015;6:10076 pubmed publisher
  • immunoprecipitation; mouse; fig 5
Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, et al. NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nat Immunol. 2016;17:250-8 pubmed publisher
  • immunocytochemistry; rat; 1:500; fig 5
Ulbrich L, Favaloro F, Trobiani L, Marchetti V, Patel V, Pascucci T, et al. Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system. Biochem J. 2016;473:423-34 pubmed publisher
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 1
Song H, Tao L, Chen C, Pan L, Hao J, Ni Y, et al. USP17-mediated deubiquitination and stabilization of HDAC2 in cigarette smoke extract-induced inflammation. Int J Clin Exp Pathol. 2015;8:10707-15 pubmed
  • western blot; human; fig 5
Debruyne D, Bhatnagar N, Sharma B, Luther W, Moore N, Cheung N, et al. ALK inhibitor resistance in ALK(F1174L)-driven neuroblastoma is associated with AXL activation and induction of EMT. Oncogene. 2016;35:3681-91 pubmed publisher
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
Wu Y, Wang L, Bao H, Zou S, Fu C, Gong H, et al. Nrdp1S, short variant of Nrdp1, inhibits human glioma progression by increasing Nrdp1-mediated ErbB3 ubiquitination and degradation. J Cell Mol Med. 2016;20:422-9 pubmed publisher
  • immunocytochemistry; human; fig s2
  • western blot; human; fig 4
Chen X, Ji Z, Webber A, Sharrocks A. Genome-wide binding studies reveal DNA binding specificity mechanisms and functional interplay amongst Forkhead transcription factors. Nucleic Acids Res. 2016;44:1566-78 pubmed publisher
  • western blot; human; fig 3
Ozgur S, Basquin J, Kamenska A, Filipowicz W, Standart N, Conti E. Structure of a Human 4E-T/DDX6/CNOT1 Complex Reveals the Different Interplay of DDX6-Binding Proteins with the CCR4-NOT Complex. Cell Rep. 2015;13:703-711 pubmed publisher
  • proximity ligation assay; mouse; fig 3b
  • chromatin immunoprecipitation; mouse; fig 2
Han Y, Kim D, Na T, Ka N, Choi H, Lee M. RORα switches transcriptional mode of ERRγ that results in transcriptional repression of CYP2E1 under ethanol-exposure. Nucleic Acids Res. 2016;44:1095-104 pubmed publisher
  • western blot; human; fig 1
Young L, Marzio A, Pérez Durán P, Reid D, Meredith D, Roberti D, et al. TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep. 2015;13:451-459 pubmed publisher
  • immunoprecipitation; fruit fly ; 1:5000; fig 5
  • western blot; fruit fly ; 1:5000; fig 5
Sun S, Reddy B, Irvine K. Localization of Hippo signalling complexes and Warts activation in vivo. Nat Commun. 2015;6:8402 pubmed publisher
  • immunoprecipitation; human; fig 5
Guo Y, Feng W, Sy S, Huen M. ATM-dependent Phosphorylation of the Fanconi Anemia Protein PALB2 Promotes the DNA Damage Response. J Biol Chem. 2015;290:27545-56 pubmed publisher
  • western blot; human; 1:5000; fig 1
Rao X, Duan X, Mao W, Li X, Li Z, Li Q, et al. O-GlcNAcylation of G6PD promotes the pentose phosphate pathway and tumor growth. Nat Commun. 2015;6:8468 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Anderson K, Russell A, Foletta V. NDRG2 promotes myoblast proliferation and caspase 3/7 activities during differentiation, and attenuates hydrogen peroxide - But not palmitate-induced toxicity. FEBS Open Bio. 2015;5:668-81 pubmed publisher
  • western blot; human; 1:1000; fig 1c
Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, et al. The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 2015;11:e1005503 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse; fig 3
Bender J, Engeholm M, Ederer M, Breu J, Møller T, Michalakis S, et al. Corticotropin-Releasing Hormone Receptor Type 1 (CRHR1) Clustering with MAGUKs Is Mediated via Its C-Terminal PDZ Binding Motif. PLoS ONE. 2015;10:e0136768 pubmed publisher
  • western blot; human; 1:1000; fig 8
Yang F, Yu X, Liu C, Qu C, Gong Z, Liu H, et al. Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR. Nat Commun. 2015;6:8202 pubmed publisher
  • immunoprecipitation; human; fig s1c,d
  • immunocytochemistry; human; 1:500; fig 2e
  • western blot; human; 1:4000; fig 2c
Zhang J, Tripathi D, Jing J, Alexander A, Kim J, Powell R, et al. ATM functions at the peroxisome to induce pexophagy in response to ROS. Nat Cell Biol. 2015;17:1259-1269 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Lee Y, Min C, Kim T, Song H, Lim Y, Kim D, et al. Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter. EMBO Rep. 2015;16:1318-33 pubmed publisher
  • immunoprecipitation; human
Yao X, Tang Z, Fu X, Yin J, Liang Y, Li C, et al. The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination. EMBO J. 2015;34:2885-902 pubmed publisher
  • immunoprecipitation; human; fig 5b
  • western blot; human; 1:3000; fig 5b
Jing J, He L, Sun A, Quintana A, Ding Y, Ma G, et al. Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca²⁺ influx. Nat Cell Biol. 2015;17:1339-47 pubmed publisher
  • western blot; human; 1:5000; fig s11
Xu D, Wang Z, Zhang Y, Jiang W, Pan Y, Song B, et al. PAQR3 modulates cholesterol homeostasis by anchoring Scap/SREBP complex to the Golgi apparatus. Nat Commun. 2015;6:8100 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Yu C, Ji S, Sha Q, Sun Q, Fan H. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun. 2015;6:8017 pubmed publisher
  • immunoprecipitation; human; fig 9.b,c
Li M, Zhou L, Lam H. Paraformaldehyde Fixation May Lead to Misinterpretation of the Subcellular Localization of Plant High Mobility Group Box Proteins. PLoS ONE. 2015;10:e0135033 pubmed publisher
  • western blot; human; fig 1
Steingruber M, Socher E, Hutterer C, Webel R, Bergbrede T, Lenac T, et al. The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain. Viruses. 2015;7:4582-601 pubmed publisher
  • immunoprecipitation; human; fig 9.b,c
Takata K, Tomida J, Reh S, Swanhart L, Takata M, Hukriede N, et al. Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN). J Biol Chem. 2015;290:24278-93 pubmed publisher
  • immunoprecipitation; human; fig 3
Thandapani P, Song J, Gandin V, Cai Y, Rouleau S, Garant J, et al. Aven recognition of RNA G-quadruplexes regulates translation of the mixed lineage leukemia protooncogenes. elife. 2015;4: pubmed publisher
  • western blot; mouse; fig 2d
  • immunocytochemistry; African green monkey; fig 5c
Zhen Y, Li W. Impairment of autophagosome-lysosome fusion in the buff mutant mice with the VPS33A(D251E) mutation. Autophagy. 2015;11:1608-22 pubmed publisher
  • immunohistochemistry; human; 1:2000; fig 4
He S, Zhao Z, Yang Y, O Connell D, Zhang X, Oh S, et al. Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nat Commun. 2015;6:7839 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Lee C, Yang Y, Chen C, Liu J. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene. 2016;35:1988-95 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 5
  • western blot; human; 1:20,000; fig 5
Gentili C, Castor D, Kaden S, Lauterbach D, Gysi M, Steigemann P, et al. Chromosome Missegregation Associated with RUVBL1 Deficiency. PLoS ONE. 2015;10:e0133576 pubmed publisher
  • western blot; rat; fig 6a
Bollimpelli V, Kondapi A. Enriched rat primary ventral mesencephalic neurons as an in-vitro culture model. Neuroreport. 2015;26:728-34 pubmed publisher
  • western blot; rat; fig 6a
Pedros C, Gaud G, Bernard I, Kassem S, Chabod M, Lagrange D, et al. An Epistatic Interaction between Themis1 and Vav1 Modulates Regulatory T Cell Function and Inflammatory Bowel Disease Development. J Immunol. 2015;195:1608-16 pubmed publisher
  • western blot; human; fig 6
Xu Q, Zhang Y, Wei Q, Huang Y, Li Y, Ling K, et al. BBS4 and BBS5 show functional redundancy in the BBSome to regulate the degradative sorting of ciliary sensory receptors. Sci Rep. 2015;5:11855 pubmed publisher
  • immunocytochemistry; roundworm ; fig 6b
Blanchette C, Perrat P, Thackeray A, Benard C. Glypican Is a Modulator of Netrin-Mediated Axon Guidance. PLoS Biol. 2015;13:e1002183 pubmed publisher
  • western blot; human; loading ...; fig 3a
Loveless T, Topacio B, Vashisht A, Galaang S, Ulrich K, Young B, et al. DNA Damage Regulates Translation through β-TRCP Targeting of CReP. PLoS Genet. 2015;11:e1005292 pubmed publisher
  • immunohistochemistry; mouse; 1:6000; fig s1a
  • western blot; human; fig s1b
Tindi J, Chávez A, Cvejic S, Calvo Ochoa E, Castillo P, Jordan B. ANKS1B Gene Product AIDA-1 Controls Hippocampal Synaptic Transmission by Regulating GluN2B Subunit Localization. J Neurosci. 2015;35:8986-96 pubmed publisher
  • immunoprecipitation; human; fig 3
Song H, Pu J, Wang L, Wu L, Xiao J, Liu Q, et al. ATG16L1 phosphorylation is oppositely regulated by CSNK2/casein kinase 2 and PPP1/protein phosphatase 1 which determines the fate of cardiomyocytes during hypoxia/reoxygenation. Autophagy. 2015;11:1308-25 pubmed publisher
  • immunohistochemistry; mouse; 1:6000; fig s1a
  • western blot; human; fig s1b
Omori Y, Chaya T, Yoshida S, Irie S, Tsujii T, Furukawa T. Identification of G Protein-Coupled Receptors (GPCRs) in Primary Cilia and Their Possible Involvement in Body Weight Control. PLoS ONE. 2015;10:e0128422 pubmed publisher
  • western blot; human; fig 5
Nicolai S, Filippi S, Caputo M, Cipak L, Gregan J, Ammerer G, et al. Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics. PLoS ONE. 2015;10:e0128558 pubmed publisher
  • immunoprecipitation; human; fig 1
Lu H, Li Z, Zhang W, Schulze Gahmen U, Xue Y, Zhou Q. Gene target specificity of the Super Elongation Complex (SEC) family: how HIV-1 Tat employs selected SEC members to activate viral transcription. Nucleic Acids Res. 2015;43:5868-79 pubmed publisher
  • chromatin immunoprecipitation; human; 1:200; fig 5
  • western blot; human; 1:5000; fig 5
Zhao Z, Sagare A, Ma Q, Halliday M, Kong P, Kisler K, et al. Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance. Nat Neurosci. 2015;18:978-87 pubmed publisher
  • immunocytochemistry; mouse; fig s1
Jackson B, Ivanova I, Dagnino L. An ELMO2-RhoG-ILK network modulates microtubule dynamics. Mol Biol Cell. 2015;26:2712-25 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Iglesias Bartolomé R, Torres D, Marone R, Feng X, Martin D, Simaan M, et al. Inactivation of a Gα(s)-PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesis. Nat Cell Biol. 2015;17:793-803 pubmed publisher
  • western blot; human; fig s1
Ibsen M, Gad H, Andersen L, Hornung V, Julkunen I, Sarkar S, et al. Structural and functional analysis reveals that human OASL binds dsRNA to enhance RIG-I signaling. Nucleic Acids Res. 2015;43:5236-48 pubmed publisher
  • western blot; human; fig 1
Park J, Zhao L, Willingham M, Cheng S. Oncogenic mutations of thyroid hormone receptor β. Oncotarget. 2015;6:8115-31 pubmed
  • western blot; human; fig 6
Cattoglio C, Zhang E, Grubisic I, Chiba K, Fong Y, Tjian R. Functional and mechanistic studies of XPC DNA-repair complex as transcriptional coactivator in embryonic stem cells. Proc Natl Acad Sci U S A. 2015;112:E2317-26 pubmed publisher
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
González Prieto R, Cuijpers S, Kumar R, Hendriks I, Vertegaal A. c-Myc is targeted to the proteasome for degradation in a SUMOylation-dependent manner, regulated by PIAS1, SENP7 and RNF4. Cell Cycle. 2015;14:1859-72 pubmed publisher
  • western blot; E. coli; fig 1,3,5,6,7
Mondal A, Potts G, Dawson A, Coon J, Mehle A. Phosphorylation at the homotypic interface regulates nucleoprotein oligomerization and assembly of the influenza virus replication machinery. PLoS Pathog. 2015;11:e1004826 pubmed publisher
  • ChIP-Seq; mouse; 1:2000; fig 5
Alexander J, Hota S, He D, Thomas S, Ho L, Pennacchio L, et al. Brg1 modulates enhancer activation in mesoderm lineage commitment. Development. 2015;142:1418-30 pubmed publisher
  • immunoprecipitation; human; 1:10,000; fig 3a
Freischmidt A, Wieland T, Richter B, Ruf W, Schaeffer V, Müller K, et al. Haploinsufficiency of TBK1 causes familial ALS and fronto-temporal dementia. Nat Neurosci. 2015;18:631-6 pubmed publisher
  • western blot; human; fig 5
Kobayashi A, Hashizume C, Dowaki T, Wong R. Therapeutic potential of mitotic interaction between the nucleoporin Tpr and aurora kinase A. Cell Cycle. 2015;14:1447-58 pubmed publisher
  • western blot; human; fig 6
Hendriks I, Treffers L, Verlaan de Vries M, Olsen J, Vertegaal A. SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. Cell Rep. 2015;10:1778-1791 pubmed publisher
  • western blot; human; 1:1000; fig 1
Xing M, Yang M, Huo W, Feng F, Wei L, Jiang W, et al. Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. Nat Commun. 2015;6:6233 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig s9
  • immunoprecipitation; human; 1:50; fig 4
Fillmore C, Xu C, Desai P, Berry J, Rowbotham S, Lin Y, et al. EZH2 inhibition sensitizes BRG1 and EGFR mutant lung tumours to TopoII inhibitors. Nature. 2015;520:239-42 pubmed publisher
  • western blot; human
Verma R, Marchese A. The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR. J Biol Chem. 2015;290:6810-24 pubmed publisher
  • western blot; human; fig 4
Lindsey Boltz L, Kemp M, Capp C, Sancar A. RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling. Cell Cycle. 2015;14:99-108 pubmed publisher
  • western blot; mouse; fig 5
Wijeweera A, Haj M, Feldman A, Pnueli L, Luo Z, Melamed P. Gonadotropin gene transcription is activated by menin-mediated effects on the chromatin. Biochim Biophys Acta. 2015;1849:328-41 pubmed publisher
  • western blot; mouse
Kanazawa I, Canaff L, Abi Rafeh J, Angrula A, Li J, Riddle R, et al. Osteoblast menin regulates bone mass in vivo. J Biol Chem. 2015;290:3910-24 pubmed publisher
  • immunoprecipitation; mouse; 1:500-1:1000; fig 7
Imajo M, Ebisuya M, Nishida E. Dual role of YAP and TAZ in renewal of the intestinal epithelium. Nat Cell Biol. 2015;17:7-19 pubmed publisher
  • western blot; human; fig 4
Zou J, Zhang D, Qin G, Chen X, Wang H, Zhang D. BRCA1 and FancJ cooperatively promote interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1. Cell Cycle. 2014;13:3685-97 pubmed publisher
  • western blot; human; 50 ng/ml; fig 4
Freund A, Zhong F, Venteicher A, Meng Z, Veenstra T, Frydman J, et al. Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1. Cell. 2014;159:1389-403 pubmed publisher
  • western blot; human
Kuo P, Huang C, Lee C, Chang H, Hsieh S, Chung Y, et al. BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability. Br J Cancer. 2015;112:391-402 pubmed publisher
  • immunocytochemistry; human; 1:5000
  • western blot; human; 1:1000
Kitai Y, Takeuchi O, Kawasaki T, Ori D, Sueyoshi T, Murase M, et al. Negative regulation of melanoma differentiation-associated gene 5 (MDA5)-dependent antiviral innate immune responses by Arf-like protein 5B. J Biol Chem. 2015;290:1269-80 pubmed publisher
  • immunoprecipitation; human; 1:5000; fig s8
Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher
  • western blot; mouse; fig 8
Goren I, Pfeilschifter J, Frank S. Uptake of neutrophil-derived Ym1 protein distinguishes wound macrophages in the absence of interleukin-4 signaling in murine wound healing. Am J Pathol. 2014;184:3249-61 pubmed publisher
  • immunoprecipitation; human; 1:100
Liang Y, Eng W, Colquhoun D, Dinglasan R, Graham D, Mahal L. Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J Biol Chem. 2014;289:32526-37 pubmed publisher
  • immunohistochemistry; rat; 1:2000
  • western blot; rat; 1:1,000
  • western blot; human; 1:1,000
Young D, Fong D, Lawlor P, Wu A, Mouravlev A, McRae M, et al. Adenosine kinase, glutamine synthetase and EAAT2 as gene therapy targets for temporal lobe epilepsy. Gene Ther. 2014;21:1029-40 pubmed publisher
  • western blot; human; loading ...; fig 6a
Merabova N, Sariyer I, Saribas A, Knezevic T, Gordon J, Turco M, et al. WW domain of BAG3 is required for the induction of autophagy in glioma cells. J Cell Physiol. 2015;230:831-41 pubmed publisher
  • western blot; human; fig 1,2,3,4,5,6
Cherepanova N, Shrimal S, Gilmore R. Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins. J Cell Biol. 2014;206:525-39 pubmed publisher
  • chromatin immunoprecipitation; human; fig 1
Deng W, Rupon J, Krivega I, Breda L, Motta I, Jahn K, et al. Reactivation of developmentally silenced globin genes by forced chromatin looping. Cell. 2014;158:849-860 pubmed publisher
  • western blot; mouse; fig s5
Xu R, Hu Q, Ma Q, Liu C, Wang G. The protease Omi regulates mitochondrial biogenesis through the GSK3β/PGC-1α pathway. Cell Death Dis. 2014;5:e1373 pubmed publisher
  • western blot; human; 1:2000; fig s11
Winter G, Radic B, Mayor Ruiz C, Blomen V, Trefzer C, Kandasamy R, et al. The solute carrier SLC35F2 enables YM155-mediated DNA damage toxicity. Nat Chem Biol. 2014;10:768-773 pubmed publisher
  • immunoprecipitation; mouse; fig 1
Booth A, Tarafder A, Hume A, Recchi C, Seabra M. A role for Na+,K+-ATPase ?1 in regulating Rab27a localisation on melanosomes. PLoS ONE. 2014;9:e102851 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse
  • western blot; mouse
Ro S, Semple I, Park H, Park H, Park H, Kim M, et al. Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of p62/sequestosome-1. FEBS J. 2014;281:3816-27 pubmed publisher
  • western blot; human; 2 ug/ml
Toyoda S, Yoshimura T, Mizuta J, Miyazaki J. Auto-regulation of the Sohlh1 gene by the SOHLH2/SOHLH1/SP1 complex: implications for early spermatogenesis and oogenesis. PLoS ONE. 2014;9:e101681 pubmed publisher
  • immunoprecipitation; human
Zhang P, Gao K, Tang Y, Jin X, An J, Yu H, et al. Destruction of DDIT3/CHOP protein by wild-type SPOP but not prostate cancer-associated mutants. Hum Mutat. 2014;35:1142-51 pubmed publisher
  • western blot; mouse
Wijayatunge R, Chen L, Cha Y, Zannas A, Frank C, West A. The histone lysine demethylase Kdm6b is required for activity-dependent preconditioning of hippocampal neuronal survival. Mol Cell Neurosci. 2014;61:187-200 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Choi Y, Kim Y, Jeong H, Jin Y, Yeo C, Lee K. Akt enhances Runx2 protein stability by regulating Smurf2 function during osteoblast differentiation. FEBS J. 2014;281:3656-66 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
Sun H, Liu Y, Hunter T. Multiple Arkadia/RNF111 structures coordinate its Polycomb body association and transcriptional control. Mol Cell Biol. 2014;34:2981-95 pubmed publisher
  • immunoprecipitation; human; fig 5
  • western blot; human; fig 3, 4, 5
Kobayashi T, Masoumi K, Massoumi R. Deubiquitinating activity of CYLD is impaired by SUMOylation in neuroblastoma cells. Oncogene. 2015;34:2251-60 pubmed publisher
  • western blot; human; fig 2
Hayashi A, Suenaga N, Shiomi Y, Nishitani H. PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells. Methods Mol Biol. 2014;1170:367-82 pubmed publisher
  • western blot; human; fig 3 a-c
Kadota S, Nagata K. Silencing of IFN-stimulated gene transcription is regulated by histone H1 and its chaperone TAF-I. Nucleic Acids Res. 2014;42:7642-53 pubmed publisher
  • western blot; human; fig e6
Popow J, Jurkin J, Schleiffer A, Martinez J. Analysis of orthologous groups reveals archease and DDX1 as tRNA splicing factors. Nature. 2014;511:104-7 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 5e
  • western blot; mouse; 1:1000; fig s5b
Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge N, et al. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Rep. 2014;7:1456-1470 pubmed publisher
  • immunoprecipitation; human; fig 7
Piazza I, Rutkowska A, Ori A, Walczak M, Metz J, Pelechano V, et al. Association of condensin with chromosomes depends on DNA binding by its HEAT-repeat subunits. Nat Struct Mol Biol. 2014;21:560-8 pubmed publisher
  • immunocytochemistry; human
Munoz M, Yanez D, Stark J. An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells. Nucleic Acids Res. 2014;42:7720-33 pubmed publisher
  • immunocytochemistry; human; 1:500
Hans F, Fiesel F, Strong J, J ckel S, Rasse T, Geisler S, et al. UBE2E ubiquitin-conjugating enzymes and ubiquitin isopeptidase Y regulate TDP-43 protein ubiquitination. J Biol Chem. 2014;289:19164-79 pubmed publisher
  • western blot; human
Li C, Egloff A, Sen M, Grandis J, Johnson D. Caspase-8 mutations in head and neck cancer confer resistance to death receptor-mediated apoptosis and enhance migration, invasion, and tumor growth. Mol Oncol. 2014;8:1220-30 pubmed publisher
  • western blot; human; fig s2
Duda K, Lonowski L, Kofoed Nielsen M, Ibarra A, Delay C, Kang Q, et al. High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs. Nucleic Acids Res. 2014;42:e84 pubmed publisher
  • immunocytochemistry; human
Vidi P, Liu J, Salles D, Jayaraman S, Dorfman G, Gray M, et al. NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks. Nucleic Acids Res. 2014;42:6365-79 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Cipriano R, Miskimen K, Bryson B, Foy C, Bartel C, Jackson M. Conserved oncogenic behavior of the FAM83 family regulates MAPK signaling in human cancer. Mol Cancer Res. 2014;12:1156-65 pubmed publisher
  • immunocytochemistry; human
Kerdivel G, Boudot A, Habauzit D, Percevault F, Demay F, Pakdel F, et al. Activation of the MKL1/actin signaling pathway induces hormonal escape in estrogen-responsive breast cancer cell lines. Mol Cell Endocrinol. 2014;390:34-44 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Hu B, Li S, Zhang X, Zheng X. HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation. Nucleic Acids Res. 2014;42:5582-93 pubmed publisher
  • western blot; human
Havel J, Li Z, Cheng D, Peng J, Fu H. Nuclear PRAS40 couples the Akt/mTORC1 signaling axis to the RPL11-HDM2-p53 nucleolar stress response pathway. Oncogene. 2015;34:1487-98 pubmed publisher
  • western blot; human
Rappe U, Schlechter T, Aschoff M, Hotz Wagenblatt A, Hofmann I. Nuclear ARVCF protein binds splicing factors and contributes to the regulation of alternative splicing. J Biol Chem. 2014;289:12421-34 pubmed publisher
  • flow cytometry; African green monkey; 1:1000
  • western blot; African green monkey; 1:10000
Kakiuchi K, Tsuda A, Goto Y, Shimada T, Taniguchi K, Takagishi K, et al. Cell-surface DEAD-box polypeptide 4-immunoreactive cells and gonocytes are two distinct populations in postnatal porcine testes. Biol Reprod. 2014;90:82 pubmed publisher
  • immunoprecipitation; human
Kim J, Sharma A, Dhar S, Lee S, Gu B, Chan C, et al. UTX and MLL4 coordinately regulate transcriptional programs for cell proliferation and invasiveness in breast cancer cells. Cancer Res. 2014;74:1705-17 pubmed publisher
  • immunocytochemistry; human
Salvi M, Raiborg C, Hanson P, Campsteijn C, Stenmark H, Pinna L. CK2 involvement in ESCRT-III complex phosphorylation. Arch Biochem Biophys. 2014;545:83-91 pubmed publisher
  • western blot; human; 1:10000
Heyne K, Förster J, Schüle R, Roemer K. Transcriptional repressor NIR interacts with the p53-inhibiting ubiquitin ligase MDM2. Nucleic Acids Res. 2014;42:3565-79 pubmed publisher
  • western blot; human; fig 1
Aida K, Hayashi H, Inamura K, Mizuno T, Sugiyama Y. Differential roles of ubiquitination in the degradation mechanism of cell surface-resident bile salt export pump and multidrug resistance-associated protein 2. Mol Pharmacol. 2014;85:482-91 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Zhang W, Ji W, Liu X, Ouyang G, Xiao W. ELL inhibits E2F1 transcriptional activity by enhancing E2F1 deacetylation via recruitment of histone deacetylase 1. Mol Cell Biol. 2014;34:765-75 pubmed publisher
  • immunocytochemistry; African green monkey
  • western blot; mouse
Brot S, Auger C, Bentata R, Rogemond V, Ménigoz S, Chounlamountri N, et al. Collapsin response mediator protein 5 (CRMP5) induces mitophagy, thereby regulating mitochondrion numbers in dendrites. J Biol Chem. 2014;289:2261-76 pubmed publisher
  • immunocytochemistry; human; fig 2
Wang W, Wang Y, Chen H, Xing Y, Li F, Zhang Q, et al. Orphan nuclear receptor TR3 acts in autophagic cell death via mitochondrial signaling pathway. Nat Chem Biol. 2014;10:133-40 pubmed publisher
  • western blot; human
Kim K, Park S, Na S, Kim J, Choi H, Kim Y, et al. Reinvestigation of aminoacyl-tRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS ONE. 2013;8:e81734 pubmed publisher
  • chromatin immunoprecipitation; fruit fly
Basu A, Wilkinson F, Colavita K, Fennelly C, Atchison M. YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment. Nucleic Acids Res. 2014;42:2208-23 pubmed publisher
  • immunohistochemistry - frozen section; Xenopus laevis
Chu C, Gerstenzang E, Ossipova O, Sokol S. Lulu regulates Shroom-induced apical constriction during neural tube closure. PLoS ONE. 2013;8:e81854 pubmed publisher
  • western blot; human
Lam T, Thomas L, White C, Li G, Pone E, Xu Z, et al. Scaffold functions of 14-3-3 adaptors in B cell immunoglobulin class switch DNA recombination. PLoS ONE. 2013;8:e80414 pubmed publisher
  • immunoprecipitation; human
  • western blot; human; fig s1
Alsarraj J, Faraji F, Geiger T, Mattaini K, Williams M, Wu J, et al. BRD4 short isoform interacts with RRP1B, SIPA1 and components of the LINC complex at the inner face of the nuclear membrane. PLoS ONE. 2013;8:e80746 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human; fig 2
Liu X, Xiao W, Wang X, Li Y, Han J, Li Y. The p38-interacting protein (p38IP) regulates G2/M progression by promoting ?-tubulin acetylation via inhibiting ubiquitination-induced degradation of the acetyltransferase GCN5. J Biol Chem. 2013;288:36648-61 pubmed publisher
  • western blot; human
Wu H, Balsbaugh J, Chandler H, Georgilis A, Zullow H, Shabanowitz J, et al. Mitogen-activated protein kinase signaling mediates phosphorylation of polycomb ortholog Cbx7. J Biol Chem. 2013;288:36398-408 pubmed publisher
  • western blot; mouse; 1:2000
Evans C, Cook S, Coleman M, Gilley J. MEK inhibitor U0126 reverses protection of axons from Wallerian degeneration independently of MEK-ERK signaling. PLoS ONE. 2013;8:e76505 pubmed publisher
  • immunocytochemistry; human; fig 2b
Hashizume C, Kobayashi A, Wong R. Down-modulation of nucleoporin RanBP2/Nup358 impaired chromosomal alignment and induced mitotic catastrophe. Cell Death Dis. 2013;4:e854 pubmed publisher
  • western blot; fission yeast
DeGennaro C, Alver B, Marguerat S, Stepanova E, Davis C, Bähler J, et al. Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast. Mol Cell Biol. 2013;33:4779-92 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Berndt A, Wilkinson K, Heimann M, Bishop P, Henley J. In vivo characterization of the properties of SUMO1-specific monobodies. Biochem J. 2013;456:385-95 pubmed publisher
  • immunoprecipitation; human
  • western blot; human; fig 3
Verma S, Cai Q, Kreider E, Lu J, Robertson E. Comprehensive analysis of LANA interacting proteins essential for viral genome tethering and persistence. PLoS ONE. 2013;8:e74662 pubmed publisher
  • immunoprecipitation; human
Jo S, Kim M, Park J, Kim T, Ahn Y. Txnip contributes to impaired glucose tolerance by upregulating the expression of genes involved in hepatic gluconeogenesis in mice. Diabetologia. 2013;56:2723-32 pubmed publisher
  • immunoprecipitation; human
Shats I, Gatza M, Liu B, Angus S, You L, Nevins J. FOXO transcription factors control E2F1 transcriptional specificity and apoptotic function. Cancer Res. 2013;73:6056-67 pubmed publisher
  • western blot; human
Voss M, Campbell K, Saranzewa N, Campbell D, Hastie C, Peggie M, et al. Protein phosphatase 4 is phosphorylated and inactivated by Cdk in response to spindle toxins and interacts with ?-tubulin. Cell Cycle. 2013;12:2876-87 pubmed publisher
  • immunocytochemistry; mouse; 1:1000
  • immunocytochemistry; human; 1:1000
Wang C, Low W, Liu A, Wang B. Centrosomal protein DZIP1 regulates Hedgehog signaling by promoting cytoplasmic retention of transcription factor GLI3 and affecting ciliogenesis. J Biol Chem. 2013;288:29518-29 pubmed publisher
  • immunoprecipitation; human; 1:200-1:500; fig 1
Gurkar A, Chu K, Raj L, Bouley R, Lee S, Kim Y, et al. Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress. Nat Commun. 2013;4:2189 pubmed publisher
  • western blot; human
Sy S, Jiang J, O W, Deng Y, Huen M. The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks. Nucleic Acids Res. 2013;41:8572-80 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Shan L, Li X, Liu L, Ding X, Wang Q, Zheng Y, et al. GATA3 cooperates with PARP1 to regulate CCND1 transcription through modulating histone H1 incorporation. Oncogene. 2014;33:3205-16 pubmed publisher
  • immunoprecipitation; human
Wei Q, Li J, Liu T, Tong X, Ye X. Phosphorylation of minichromosome maintenance protein 7 (MCM7) by cyclin/cyclin-dependent kinase affects its function in cell cycle regulation. J Biol Chem. 2013;288:19715-25 pubmed publisher
  • western blot; dogs
Boehlke C, Kotsis F, Buchholz B, Powelske C, Eckardt K, Walz G, et al. Kif3a guides microtubular dynamics, migration and lumen formation of MDCK cells. PLoS ONE. 2013;8:e62165 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Kubota S, Fukumoto Y, Aoyama K, Ishibashi K, Yuki R, Morinaga T, et al. Phosphorylation of KRAB-associated protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by nuclear tyrosine kinases inhibits the association of KAP1 and heterochromatin protein 1? (HP1?) with heterochromatin. J Biol Chem. 2013;288:17871-83 pubmed publisher
  • western blot; human
Yasuda Inoue M, Kuroki M, Ariumi Y. Distinct DDX DEAD-box RNA helicases cooperate to modulate the HIV-1 Rev function. Biochem Biophys Res Commun. 2013;434:803-8 pubmed publisher
  • western blot; human; fig 4
Tinnikov A, Samuels H. A novel cell lysis approach reveals that caspase-2 rapidly translocates from the nucleus to the cytoplasm in response to apoptotic stimuli. PLoS ONE. 2013;8:e61085 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
Vogt D, Camus G, Herker E, Webster B, Tsou C, Greene W, et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog. 2013;9:e1003302 pubmed publisher
  • western blot; human
Gurung B, Feng Z, Iwamoto D, Thiel A, Jin G, Fan C, et al. Menin epigenetically represses Hedgehog signaling in MEN1 tumor syndrome. Cancer Res. 2013;73:2650-8 pubmed publisher
  • immunoprecipitation; mouse
Zhang J, Twelvetrees A, Lazarus J, Blasier K, Yao X, Inamdar N, et al. Establishing a novel knock-in mouse line for studying neuronal cytoplasmic dynein under normal and pathologic conditions. Cytoskeleton (Hoboken). 2013;70:215-27 pubmed publisher
  • chromatin immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human; fig 8
Martin N, Popov N, Aguilo F, O Loghlen A, Raguz S, Snijders A, et al. Interplay between Homeobox proteins and Polycomb repressive complexes in p16INK?a regulation. EMBO J. 2013;32:982-95 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Lin S, Shen H, Li J, Tang S, Gu Y, Chen Z, et al. Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program. J Biol Chem. 2013;288:6238-47 pubmed publisher
  • immunocytochemistry; chicken; 1:500
Wang Y, Dantas T, Lalor P, Dockery P, Morrison C. Promoter hijack reveals pericentrin functions in mitosis and the DNA damage response. Cell Cycle. 2013;12:635-46 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Dettmer U, Newman A, Luth E, Bartels T, Selkoe D. In vivo cross-linking reveals principally oligomeric forms of ?-synuclein and ?-synuclein in neurons and non-neural cells. J Biol Chem. 2013;288:6371-85 pubmed publisher
  • western blot; chicken
  • immunoprecipitation; human
  • western blot; human; fig s10
Moreno Garcia M, Sommer K, Rincón Arano H, Brault M, Ninomiya Tsuji J, Matesic L, et al. Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-?B activation. Mol Cell Biol. 2013;33:1149-63 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Deguchi K, Nagamatsu G, Miyachi H, Kato Y, Morita S, Kimura H, et al. Posttranscriptional regulation of histone lysine methyltransferase GLP in embryonic male mouse germ cells. Biol Reprod. 2013;88:36 pubmed publisher
  • western blot; human
Morlando M, Dini Modigliani S, Torrelli G, Rosa A, Di Carlo V, Caffarelli E, et al. FUS stimulates microRNA biogenesis by facilitating co-transcriptional Drosha recruitment. EMBO J. 2012;31:4502-10 pubmed publisher
  • immunocytochemistry; African green monkey; 1:2000
  • western blot; African green monkey; 1:4000
Varco Merth B, Mirza K, Alzhanov D, Chia D, Rotwein P. Biochemical characterization of diverse Stat5b-binding enhancers that mediate growth hormone-activated insulin-like growth factor-I gene transcription. PLoS ONE. 2012;7:e50278 pubmed publisher
  • immunoprecipitation; human
  • immunoprecipitation; mouse
Wang C, Kao Y, Lai P, Chen W, Chung B. Steroidogenic factor 1 (NR5A1) maintains centrosome homeostasis in steroidogenic cells by restricting centrosomal DNA-dependent protein kinase activation. Mol Cell Biol. 2013;33:476-84 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Isaacs J, Antony L, Dalrymple S, Brennen W, Gerber S, Hammers H, et al. Tasquinimod Is an Allosteric Modulator of HDAC4 survival signaling within the compromised cancer microenvironment. Cancer Res. 2013;73:1386-99 pubmed publisher
  • western blot; human
Journo C, Bonnet A, Favre Bonvin A, Turpin J, Vinera J, C t E, et al. Human T cell leukemia virus type 2 tax-mediated NF-?B activation involves a mechanism independent of Tax conjugation to ubiquitin and SUMO. J Virol. 2013;87:1123-36 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Katoh H, Okamoto T, Fukuhara T, Kambara H, Morita E, Mori Y, et al. Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation. J Virol. 2013;87:489-502 pubmed publisher
  • immunocytochemistry; mouse
Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro R. Functional dissection of Pax3 in paraxial mesoderm development and myogenesis. Stem Cells. 2013;31:59-70 pubmed publisher
  • western blot; fission yeast
Limbo O, Moiani D, Kertokalio A, Wyman C, Tainer J, Russell P. Mre11 ATLD17/18 mutation retains Tel1/ATM activity but blocks DNA double-strand break repair. Nucleic Acids Res. 2012;40:11435-49 pubmed publisher
  • western blot; human; fig 5a
Blackford J, Guo C, Zhu R, Dougherty E, Chow C, Simons S. Identification of location and kinetically defined mechanism of cofactors and reporter genes in the cascade of steroid-regulated transactivation. J Biol Chem. 2012;287:40982-95 pubmed publisher
  • western blot; human
Lecat A, Di Valentin E, Somja J, Jourdan S, Fillet M, Kufer T, et al. The c-Jun N-terminal kinase (JNK)-binding protein (JNKBP1) acts as a negative regulator of NOD2 protein signaling by inhibiting its oligomerization process. J Biol Chem. 2012;287:29213-26 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Boulay G, Dubuissez M, Van Rechem C, Forget A, Helin K, Ayrault O, et al. Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins. J Biol Chem. 2012;287:10509-24 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Zhou F, Zhang X, van Dam H, Ten Dijke P, Huang H, Zhang L. Ubiquitin-specific protease 4 mitigates Toll-like/interleukin-1 receptor signaling and regulates innate immune activation. J Biol Chem. 2012;287:11002-10 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Heinz L, Rebsamen M, Rossi D, Staehli F, Schroder K, Quadroni M, et al. The death domain-containing protein Unc5CL is a novel MyD88-independent activator of the pro-inflammatory IRAK signaling cascade. Cell Death Differ. 2012;19:722-31 pubmed publisher
  • western blot; human; fig 5
Wan F, Weaver A, Gao X, Bern M, Hardwidge P, Lenardo M. IKK? phosphorylation regulates RPS3 nuclear translocation and NF-?B function during infection with Escherichia coli strain O157:H7. Nat Immunol. 2011;12:335-43 pubmed publisher
  • immunoprecipitation; Saccharomycetales
Chandrasekharan M, Huang F, Sun Z. Decoding the trans-histone crosstalk: methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors. Methods. 2011;54:304-14 pubmed publisher
  • chromatin immunoprecipitation; roundworm ; 2-5 ug
Rechtsteiner A, Ercan S, Takasaki T, Phippen T, Egelhofer T, Wang W, et al. The histone H3K36 methyltransferase MES-4 acts epigenetically to transmit the memory of germline gene expression to progeny. PLoS Genet. 2010;6:e1001091 pubmed publisher
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product information
Catalog Number :
F3165
Product Name :
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
Product Type :
ELISAS
Product Group :
Immunoassay Platform Solutions
Product Description :
clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA