This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal ANTI-FLAG® M1 antibody produced in mouse
catalog :
F3040
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M1
reactivity :
human
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 91
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 6c
Yuan M, Xie F, Xia X, Zhong K, Lian L, Zhang S, et al. UNC5C‑knockdown enhances the growth and metastasis of breast cancer cells by potentiating the integrin α6/β4 signaling pathway. Int J Oncol. 2019;: pubmed publisher
  • western blot; human; 1:2000
Nie C, Luo Y, Zhao X, Luo N, Tong A, Liu X, et al. Caspase-9 mediates Puma activation in UCN-01-induced apoptosis. Cell Death Dis. 2014;5:e1495 pubmed publisher
  • western blot; human; 1 ug/ml
Möykkynen T, Coleman S, Semenov A, Keinanen K. The N-terminal domain modulates ?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor desensitization. J Biol Chem. 2014;289:13197-205 pubmed publisher
Niu W, Luo Y, Zhou Y, Li M, Wu C, Duan Y, et al. BRD7 suppresses invasion and metastasis in breast cancer by negatively regulating YB1-induced epithelial-mesenchymal transition. J Exp Clin Cancer Res. 2020;39:30 pubmed publisher
Shen A, Chen D, Kaur M, Bartels P, Xu B, Shi Q, et al. β-blockers augment L-type Ca2+ channel activity by targeting spatially restricted β2AR signaling in neurons. elife. 2019;8: pubmed publisher
Rosendale M, Van T, Grillo Bosch D, Sposini S, Claverie L, Gauthereau I, et al. Functional recruitment of dynamin requires multimeric interactions for efficient endocytosis. Nat Commun. 2019;10:4462 pubmed publisher
Sakaguchi M, Cai W, Wang C, Cederquist C, Damasio M, Homan E, et al. FoxK1 and FoxK2 in insulin regulation of cellular and mitochondrial metabolism. Nat Commun. 2019;10:1582 pubmed publisher
Tsai C, Kuo F, Lee S, Chang Y, Fu H. Endocytosis-dependent lysosomal degradation of Src induced by protease-activated receptor 1. FEBS Lett. 2019;593:504-517 pubmed publisher
Li Y, Hu Q, Li C, Liang K, Xiang Y, Hsiao H, et al. PTEN-induced partial epithelial-mesenchymal transition drives diabetic kidney disease. J Clin Invest. 2019;129:1129-1151 pubmed publisher
Yan Y, Rato C, Rohland L, Preissler S, Ron D. MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP. Nat Commun. 2019;10:541 pubmed publisher
van der Klaauw A, Croizier S, Mendes de Oliveira E, Stadler L, Park S, Kong Y, et al. Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance. Cell. 2019;176:729-742.e18 pubmed publisher
Srikanth S, Woo J, Wu B, El Sherbiny Y, Leung J, Chupradit K, et al. The Ca2+ sensor STIM1 regulates the type I interferon response by retaining the signaling adaptor STING at the endoplasmic reticulum. Nat Immunol. 2019;20:152-162 pubmed publisher
Shiwarski D, Crilly S, Dates A, Puthenveedu M. Dual RXR motifs regulate nerve growth factor-mediated intracellular retention of the delta opioid receptor. Mol Biol Cell. 2019;30:680-690 pubmed publisher
Albakri M, Jiang Y, Genereaux J, Lajoie P. Polyglutamine toxicity assays highlight the advantages of mScarlet for imaging in Saccharomyces cerevisiae. F1000Res. 2018;7:1242 pubmed publisher
Eichel K, Jullié D, Barsi Rhyne B, Latorraca N, Masureel M, Sibarita J, et al. Catalytic activation of β-arrestin by GPCRs. Nature. 2018;557:381-386 pubmed publisher
Zhu K, Liang W, Ma Z, Xu D, Cao S, Lu X, et al. Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression. Cell Death Dis. 2018;9:500 pubmed publisher
Yang Z, Han S, Keller M, Kaiser A, Bender B, Bosse M, et al. Structural basis of ligand binding modes at the neuropeptide Y Y1 receptor. Nature. 2018;556:520-524 pubmed publisher
Sato K, Yamashita T, Ohuchi H, Takeuchi A, Gotoh H, Ono K, et al. Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor. Nat Commun. 2018;9:1255 pubmed publisher
Barford K, Keeler A, McMahon L, McDaniel K, Yap C, Deppmann C, et al. Transcytosis of TrkA leads to diversification of dendritic signaling endosomes. Sci Rep. 2018;8:4715 pubmed publisher
Shen A, Nieves Cintrón M, Deng Y, Shi Q, Chowdhury D, Qi J, et al. Functionally distinct and selectively phosphorylated GPCR subpopulations co-exist in a single cell. Nat Commun. 2018;9:1050 pubmed publisher
Di Costanzo A, Del Gaudio N, Conte L, Dell aversana C, Vermeulen M, De The H, et al. The HDAC inhibitor SAHA regulates CBX2 stability via a SUMO-triggered ubiquitin-mediated pathway in leukemia. Oncogene. 2018;37:2559-2572 pubmed publisher
Grundmann M, Merten N, Malfacini D, Inoue A, Preis P, Simon K, et al. Lack of beta-arrestin signaling in the absence of active G proteins. Nat Commun. 2018;9:341 pubmed publisher
Siljee J, Wang Y, Bernard A, Ersoy B, Zhang S, Marley A, et al. Subcellular localization of MC4R with ADCY3 at neuronal primary cilia underlies a common pathway for genetic predisposition to obesity. Nat Genet. 2018;50:180-185 pubmed publisher
Sica V, Bravo San Pedro J, Chen G, Mariño G, Lachkar S, Izzo V, et al. Inhibitor of growth protein 4 interacts with Beclin 1 and represses autophagy. Oncotarget. 2017;8:89527-89538 pubmed publisher
Manandhar D, Song L, Kabadi A, Kwon J, Edsall L, Ehrlich M, et al. Incomplete MyoD-induced transdifferentiation is associated with chromatin remodeling deficiencies. Nucleic Acids Res. 2017;45:11684-11699 pubmed publisher
Pu J, Mao Y, Xu L, Zheng T, Zhang B. Stable cell lines of human SH-SY5Y uniformly expressing wild-type or mutant-type FERM domain containing 7 gene. Exp Ther Med. 2017;14:2277-2283 pubmed publisher
Dall Acqua A, Sonego M, Pellizzari I, Pellarin I, Canzonieri V, D Andrea S, et al. CDK6 protects epithelial ovarian cancer from platinum-induced death via FOXO3 regulation. EMBO Mol Med. 2017;9:1415-1433 pubmed publisher
Xu Y, Liang Z, Li C, Yang Z, Chen L. LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells. Mol Med Rep. 2017;16:4159-4164 pubmed publisher
Xu J, Xu X, Wang B, Ma Y, Zhang L, Xu H, et al. Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. Proc Natl Acad Sci U S A. 2017;114:8620-8625 pubmed publisher
Shiwarski D, Darr M, Telmer C, Bruchez M, Puthenveedu M. PI3K class II ? regulates ?-opioid receptor export from the trans-Golgi network. Mol Biol Cell. 2017;28:2202-2219 pubmed publisher
Nakatsu Y, Mori K, Matsunaga Y, Yamamotoya T, Ueda K, Inoue Y, et al. The prolyl isomerase Pin1 increases β-cell proliferation and enhances insulin secretion. J Biol Chem. 2017;292:11886-11895 pubmed publisher
Saarenpää T, Kogan K, Sidorova Y, Mahato A, Tascón I, Kaljunen H, et al. Zebrafish GDNF and its co-receptor GFR?1 activate the human RET receptor and promote the survival of dopaminergic neurons in vitro. PLoS ONE. 2017;12:e0176166 pubmed publisher
Julius A, Laur L, Schanzenbach C, Langosch D. BLaTM 2.0, a Genetic Tool Revealing Preferred Antiparallel Interaction of Transmembrane Helix 4 of the Dual-Topology Protein EmrE. J Mol Biol. 2017;429:1630-1637 pubmed publisher
Lobingier B, Hüttenhain R, Eichel K, Miller K, Ting A, von Zastrow M, et al. An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Cell. 2017;169:350-360.e12 pubmed publisher
Temkin P, Morishita W, Goswami D, Arendt K, Chen L, Malenka R. The Retromer Supports AMPA Receptor Trafficking During LTP. Neuron. 2017;94:74-82.e5 pubmed publisher
Lehigh K, West K, Ginty D. Retrogradely Transported TrkA Endosomes Signal Locally within Dendrites to Maintain Sympathetic Neuron Synapses. Cell Rep. 2017;19:86-100 pubmed publisher
Schanzenbach C, Schmidt F, Breckner P, Teese M, Langosch D. Identifying ionic interactions within a membrane using BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Sci Rep. 2017;7:43476 pubmed publisher
Shiwarski D, Tipton A, Giraldo M, Schmidt B, Gold M, Pradhan A, et al. A PTEN-Regulated Checkpoint Controls Surface Delivery of ? Opioid Receptors. J Neurosci. 2017;37:3741-3752 pubmed publisher
Burk K, Mire E, Bellon A, Hocine M, Guillot J, Moraes F, et al. Post-endocytic sorting of Plexin-D1 controls signal transduction and development of axonal and vascular circuits. Nat Commun. 2017;8:14508 pubmed publisher
Pons M, Izquierdo I, Andreu Carbó M, Garrido G, Planaguma J, Muriel O, et al. Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling. J Cell Sci. 2017;130:490-501 pubmed publisher
Lebeau P, Al Hashimi A, Sood S, Lhotak S, Yu P, Gyulay G, et al. Endoplasmic Reticulum Stress and Ca2+ Depletion Differentially Modulate the Sterol Regulatory Protein PCSK9 to Control Lipid Metabolism. J Biol Chem. 2017;292:1510-1523 pubmed publisher
Ubelmann F, Burrinha T, Salavessa L, Gomes R, Ferreira C, Moreno N, et al. Bin1 and CD2AP polarise the endocytic generation of beta-amyloid. EMBO Rep. 2017;18:102-122 pubmed publisher
Nelson V, Ali A, Dutta N, Ghosh S, Jana M, Ganguli A, et al. Azadiradione ameliorates polyglutamine expansion disease in Drosophila by potentiating DNA binding activity of heat shock factor 1. Oncotarget. 2016;7:78281-78296 pubmed publisher
Jiang Y, Di Gregorio S, Duennwald M, Lajoie P. Polyglutamine toxicity in yeast uncovers phenotypic variations between different fluorescent protein fusions. Traffic. 2017;18:58-70 pubmed publisher
Kuramoto K, Wang N, Fan Y, Zhang W, Schoenen F, Frankowski K, et al. Autophagy activation by novel inducers prevents BECN2-mediated drug tolerance to cannabinoids. Autophagy. 2016;12:1460-71 pubmed publisher
Timms R, Menzies S, Tchasovnikarova I, Christensen L, Williamson J, Antrobus R, et al. Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens. Nat Commun. 2016;7:11786 pubmed publisher
Tian X, Irannejad R, Bowman S, Du Y, Puthenveedu M, von Zastrow M, et al. The ?-Arrestin ARRDC3 Regulates the Endosomal Residence Time and Intracellular Signaling of the ?2-Adrenergic Receptor. J Biol Chem. 2016;291:14510-25 pubmed publisher
Alameda J, Gaspar M, Ramirez A, Navarro M, Page A, Suárez Cabrera C, et al. Deciphering the role of nuclear and cytoplasmic IKK? in skin cancer. Oncotarget. 2016;7:29531-47 pubmed publisher
Xia P, Ye B, Wang S, Zhu X, Du Y, Xiong Z, et al. Glutamylation of the DNA sensor cGAS regulates its binding and synthase activity in antiviral immunity. Nat Immunol. 2016;17:369-78 pubmed publisher
Eichel K, Jullié D, von Zastrow M. β-Arrestin drives MAP kinase signalling from clathrin-coated structures after GPCR dissociation. Nat Cell Biol. 2016;18:303-10 pubmed publisher
Grimsey N, Coronel L, Cordova I, Trejo J. Recycling and Endosomal Sorting of Protease-activated Receptor-1 Is Distinctly Regulated by Rab11A and Rab11B Proteins. J Biol Chem. 2016;291:2223-36 pubmed publisher
Zhou B, Li H, Liu J, Xu L, Guo Q, Sun H, et al. Progranulin induces adipose insulin resistance and autophagic imbalance via TNFR1 in mice. J Mol Endocrinol. 2015;55:231-43 pubmed publisher
Krishnan N, Krishnan K, Connors C, Choy M, Page R, Peti W, et al. PTP1B inhibition suggests a therapeutic strategy for Rett syndrome. J Clin Invest. 2015;125:3163-77 pubmed publisher
Clavier A, Ruby V, Rincheval Arnold A, Mignotte B, Guenal I. The Drosophila retinoblastoma protein, Rbf1, induces a Debcl- and Drp1-dependent mitochondrial apoptosis. J Cell Sci. 2015;128:3239-49 pubmed publisher
Sjögren B, Swaney S, Neubig R. FBXO44-Mediated Degradation of RGS2 Protein Uniquely Depends on a Cullin 4B/DDB1 Complex. PLoS ONE. 2015;10:e0123581 pubmed publisher
Weems J, Slaughter B, Unruh J, Hall S, McLaird M, Gilmore J, et al. Assembly of the Elongin A Ubiquitin Ligase Is Regulated by Genotoxic and Other Stresses. J Biol Chem. 2015;290:15030-41 pubmed publisher
Wattanachanya L, Wang L, Millard S, Lu W, O Carroll D, Hsiao E, et al. Assessing the osteoblast transcriptome in a model of enhanced bone formation due to constitutive Gs-G protein signaling in osteoblasts. Exp Cell Res. 2015;333:289-302 pubmed publisher
Zelin E, Freeman B. Lysine deacetylases regulate the heat shock response including the age-associated impairment of HSF1. J Mol Biol. 2015;427:1644-54 pubmed publisher
Best A, James K, Dalgliesh C, Hong E, Kheirolahi Kouhestani M, Curk T, et al. Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons. Nat Commun. 2014;5:4760 pubmed publisher
Cooley C, Ryno L, Plate L, Morgan G, Hulleman J, Kelly J, et al. Unfolded protein response activation reduces secretion and extracellular aggregation of amyloidogenic immunoglobulin light chain. Proc Natl Acad Sci U S A. 2014;111:13046-51 pubmed publisher
Xia P, Wang S, Huang G, Du Y, Zhu P, Li M, et al. RNF2 is recruited by WASH to ubiquitinate AMBRA1 leading to downregulation of autophagy. Cell Res. 2014;24:943-58 pubmed publisher
Martínez V, Dehò G, Simons R, Garcia Mena J. Ribonuclease PH interacts with an acidic ribonuclease E site through a basic 80-amino acid domain. FEMS Microbiol Lett. 2014;355:51-60 pubmed publisher
Grimsey N, Lin H, Trejo J. Endosomal signaling by protease-activated receptors. Methods Enzymol. 2014;535:389-401 pubmed publisher
Gaglio D, D Alfonso A, Camilloni G. Functional complementation of sir2? yeast mutation by the human orthologous gene SIRT1. PLoS ONE. 2013;8:e83114 pubmed publisher
Bossi D, Carlomagno F, Pallavicini I, Pruneri G, Trubia M, Raviele P, et al. Functional characterization of a novel FGFR1OP-RET rearrangement in hematopoietic malignancies. Mol Oncol. 2014;8:221-31 pubmed publisher
Suo D, Park J, Harrington A, Zweifel L, Mihalas S, Deppmann C. Coronin-1 is a neurotrophin endosomal effector that is required for developmental competition for survival. Nat Neurosci. 2014;17:36-45 pubmed publisher
Labrecque P, Roy S, Fréchette L, Iorio Morin C, Gallant M, Parent J. Inverse agonist and pharmacochaperone properties of MK-0524 on the prostanoid DP1 receptor. PLoS ONE. 2013;8:e65767 pubmed publisher
Li D, Dong H, Li S, Munir M, Chen J, Luo Y, et al. Hemoglobin subunit beta interacts with the capsid protein and antagonizes the growth of classical swine fever virus. J Virol. 2013;87:5707-17 pubmed publisher
Li D, Li S, Sun Y, Dong H, Li Y, Zhao B, et al. Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth. J Virol. 2013;87:2072-80 pubmed publisher
Touati S, Cladière D, Lister L, Leontiou I, Chambon J, Rattani A, et al. Cyclin A2 is required for sister chromatid segregation, but not separase control, in mouse oocyte meiosis. Cell Rep. 2012;2:1077-87 pubmed publisher
Vaughan R, Li Y, Fan B, Ranjith Kumar C, Kao C. RNA binding by the NS3 protease of the hepatitis C virus. Virus Res. 2012;169:80-90 pubmed publisher
Czech D, Lee J, Sim H, Parish C, Vilain E, Harley V. The human testis-determining factor SRY localizes in midbrain dopamine neurons and regulates multiple components of catecholamine synthesis and metabolism. J Neurochem. 2012;122:260-71 pubmed publisher
Abisambra J, Jinwal U, Suntharalingam A, Arulselvam K, Brady S, Cockman M, et al. DnaJA1 antagonizes constitutive Hsp70-mediated stabilization of tau. J Mol Biol. 2012;421:653-61 pubmed publisher
Al Sorkhy M, Ferraiuolo R, Jalili E, Malysa A, Fratiloiu A, Sloane B, et al. The cyclin-like protein Spy1/RINGO promotes mammary transformation and is elevated in human breast cancer. BMC Cancer. 2012;12:45 pubmed publisher
Hu W, Wang F, Tang J, Liu X, Yuan Z, Nie C, et al. Proapoptotic protein Smac mediates apoptosis in cisplatin-resistant ovarian cancer cells when treated with the anti-tumor agent AT101. J Biol Chem. 2012;287:68-80 pubmed publisher
Jurvansuu J, Goldman A. Obesity risk gene TMEM18 encodes a sequence-specific DNA-binding protein. PLoS ONE. 2011;6:e25317 pubmed publisher
Gapud E, Lee B, Mahowald G, Bassing C, Sleckman B. Repair of chromosomal RAG-mediated DNA breaks by mutant RAG proteins lacking phosphatidylinositol 3-like kinase consensus phosphorylation sites. J Immunol. 2011;187:1826-34 pubmed publisher
Henning M, Stiedl P, Barry D, McMahon R, Morham S, Walsh D, et al. PDZD8 is a novel moesin-interacting cytoskeletal regulatory protein that suppresses infection by herpes simplex virus type 1. Virology. 2011;415:114-21 pubmed publisher
Shi X, Liu S, Metges C, Seyfert H. C/EBP-beta drives expression of the nutritionally regulated promoter IA of the acetyl-CoA carboxylase-alpha gene in cattle. Biochim Biophys Acta. 2010;1799:561-7 pubmed publisher
Grimsey N, Graham E, Dragunow M, Glass M. Cannabinoid Receptor 1 trafficking and the role of the intracellular pool: implications for therapeutics. Biochem Pharmacol. 2010;80:1050-62 pubmed publisher
Luo M, Lu Z, Sun H, Yuan K, Zhang Q, Meng S, et al. Nuclear entry of active caspase-3 is facilitated by its p3-recognition-based specific cleavage activity. Cell Res. 2010;20:211-22 pubmed publisher
Yellaturu C, Deng X, Park E, Raghow R, Elam M. Insulin enhances the biogenesis of nuclear sterol regulatory element-binding protein (SREBP)-1c by posttranscriptional down-regulation of Insig-2A and its dissociation from SREBP cleavage-activating protein (SCAP).SREBP-1c complex. J Biol Chem. 2009;284:31726-34 pubmed publisher
Levitt E, Clark M, Jenkins P, Martens J, Traynor J. Differential effect of membrane cholesterol removal on mu- and delta-opioid receptors: a parallel comparison of acute and chronic signaling to adenylyl cyclase. J Biol Chem. 2009;284:22108-22 pubmed publisher
Yellaturu C, Deng X, Cagen L, Wilcox H, Mansbach C, Siddiqi S, et al. Insulin enhances post-translational processing of nascent SREBP-1c by promoting its phosphorylation and association with COPII vesicles. J Biol Chem. 2009;284:7518-32 pubmed publisher
Sachdev S, Bu Y, Gelman I. Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion. BMC Cancer. 2009;9:12 pubmed publisher
Berro R, Pedati C, Kehn Hall K, Wu W, Klase Z, Even Y, et al. CDK13, a new potential human immunodeficiency virus type 1 inhibitory factor regulating viral mRNA splicing. J Virol. 2008;82:7155-66 pubmed publisher
Hsiao E, Boudignon B, Chang W, Bencsik M, Peng J, Nguyen T, et al. Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass. Proc Natl Acad Sci U S A. 2008;105:1209-14 pubmed publisher
Chen M, Cai M, Aprahamian C, Georgeson K, Hruby V, Harmon C, et al. Contribution of the conserved amino acids of the melanocortin-4 receptor in [corrected] [Nle4,D-Phe7]-alpha-melanocyte-stimulating [corrected] hormone binding and signaling. J Biol Chem. 2007;282:21712-9 pubmed
Leterrier C, Laine J, Darmon M, Boudin H, Rossier J, Lenkei Z. Constitutive activation drives compartment-selective endocytosis and axonal targeting of type 1 cannabinoid receptors. J Neurosci. 2006;26:3141-53 pubmed
Van Craenenbroeck K, Clark S, Cox M, Oak J, Liu F, Van Tol H. Folding efficiency is rate-limiting in dopamine D4 receptor biogenesis. J Biol Chem. 2005;280:19350-7 pubmed
LaFleur D, Nardelli B, Tsareva T, Mather D, Feng P, Semenuk M, et al. Interferon-kappa, a novel type I interferon expressed in human keratinocytes. J Biol Chem. 2001;276:39765-71 pubmed
product information
Catalog Number :
F3040
Product Name :
Monoclonal ANTI-FLAG® M1 antibody produced in mouse
Product Type :
ELISAS
Product Group :
Immunoassay Platform Solutions
Product Description :
clone M1, purified immunoglobulin, buffered aqueous solution
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA