product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
catalog :
F1804
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M2
reactivity :
Leishmania amazonensis, African green monkey, fission yeast, Saccharomycetales, human, mouse, rat, dogs, chicken, S. cerevisiae, Drosophila melanogaster, Xenopus laevis, Caenorhabditis elegans
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, ChIP-Seq, RNA immunoprecipitation, other
more info or order :
citations: 1913
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
Alles S, Nascimento F, Lujan R, Luiz A, Millet Q, Bangash M, et al. Sensory neuron-derived NaV1.7 contributes to dorsal horn neuron excitability. Sci Adv. 2020;6:eaax4568 pubmed publisher
  • ChIP-Seq; human; loading ...; fig 2c
Lamaa A, Humbert J, Aguirrebengoa M, Cheng X, Nicolas E, Cote J, et al. Integrated analysis of H2A.Z isoforms function reveals a complex interplay in gene regulation. elife. 2020;9: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2c
Hota S, Johnson J, Verschueren E, Thomas R, Blotnick A, Zhu Y, et al. Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis. Development. 2019;: pubmed publisher
  • immunohistochemistry - paraffin section; human
  • immunohistochemistry; human
Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1b
Kanellopoulos A, Koenig J, Huang H, Pyrski M, Millet Q, Lolignier S, et al. Mapping protein interactions of sodium channel NaV1.7 using epitope-tagged gene-targeted mice. EMBO J. 2018;37:427-445 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 1c
Sasaki Y, Hidaka T, Ueno T, Akiba Takagi M, Oliva Trejo J, Seki T, et al. Sorting Nexin 9 facilitates podocin endocytosis in the injured podocyte. Sci Rep. 2017;7:43921 pubmed publisher
  • immunocytochemistry; rat; 1:50; loading ...; fig 1c
Goodman L, Baddeley D, Ambroziak W, Waites C, Garner C, Soeller C, et al. N-terminal SAP97 isoforms differentially regulate synaptic structure and postsynaptic surface pools of AMPA receptors. Hippocampus. 2017;27:668-682 pubmed publisher
  • western blot; human; loading ...; fig 1h
Deng X, Shao G, Zhang H, Li C, Zhang D, Cheng L, et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene. 2017;36:1223-1231 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 1f
Hansen R, Mund A, Poulsen S, Sandoval M, Klement K, Tsouroula K, et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat Cell Biol. 2016;18:1357-1366 pubmed publisher
  • immunocytochemistry; human
Scholefield J, Henriques R, Savulescu A, Fontan E, Boucharlat A, Laplantine E, et al. Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti. Nat Commun. 2016;7:12629 pubmed publisher
  • ChIP-Seq; S. cerevisiae
Pakchuen S, Ishibashi M, Takakusagi E, Shirahige K, Sutani T. Physical Association of Saccharomyces cerevisiae Polo-like Kinase Cdc5 with Chromosomal Cohesin Facilitates DNA Damage Response. J Biol Chem. 2016;291:17228-46 pubmed publisher
  • western blot; rat; fig 3a
Bott L, Salomons F, Maric D, Liu Y, Merry D, Fischbeck K, et al. The polyglutamine-expanded androgen receptor responsible for spinal and bulbar muscular atrophy inhibits the APC/C(Cdh1) ubiquitin ligase complex. Sci Rep. 2016;6:27703 pubmed publisher
  • immunoprecipitation; human; fig 4
Glenewinkel F, Cohen M, King C, Kaspar S, Bamberg Lemper S, Mymryk J, et al. The adaptor protein DCAF7 mediates the interaction of the adenovirus E1A oncoprotein with the protein kinases DYRK1A and HIPK2. Sci Rep. 2016;6:28241 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 2
  • western blot; mouse; fig 1
Wang H, Wang R, Carrera I, Xu S, Lakshmana M. TFEB Overexpression in the P301S Model of Tauopathy Mitigates Increased PHF1 Levels and Lipofuscin Puncta and Rescues Memory Deficits. Eneuro. 2016;3: pubmed publisher
  • chromatin immunoprecipitation; human; fig 2
  • EMSA; human; fig 2
  • immunocytochemistry; human; fig s1
Ferrán B, Martí Pàmies I, Alonso J, Rodríguez Calvo R, Aguiló S, Vidal F, et al. The nuclear receptor NOR-1 regulates the small muscle protein, X-linked (SMPX) and myotube differentiation. Sci Rep. 2016;6:25944 pubmed publisher
  • immunoprecipitation; Drosophila melanogaster; 500 ul/ml; fig 4
Le T, Vuong L, Kim A, Hsu Y, Choi K. 14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila. Nat Commun. 2016;7:11501 pubmed publisher
  • western blot; human; 1:1000; fig 2
Aizawa S, Okamoto T, Sugiyama Y, Kouwaki T, Ito A, Suzuki T, et al. TRC8-dependent degradation of hepatitis C virus immature core protein regulates viral propagation and pathogenesis. Nat Commun. 2016;7:11379 pubmed publisher
  • western blot; human; 1:1000; fig 7
Watanabe Y, Papoutsoglou P, Maturi V, Tsubakihara Y, Hottiger M, Heldin C, et al. Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation. J Biol Chem. 2016;291:12706-23 pubmed publisher
  • western blot; human; fig 4
Beard J, Tenga A, Hills J, Hoyer J, Cherian M, Wang Y, et al. The orphan nuclear receptor NR4A2 is part of a p53-microRNA-34 network. Sci Rep. 2016;6:25108 pubmed publisher
  • chromatin immunoprecipitation; human; fig s4
  • immunocytochemistry; human; fig s4
  • western blot; human; fig 1
Huang C, Cheng J, Bawa Khalfe T, Yao X, Chin Y, Yeh E. SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability. Cell Rep. 2016;15:147-157 pubmed publisher
  • immunoprecipitation; mouse; fig 4b
Kuzuya A, Zoltowska K, Post K, Arimon M, Li X, Svirsky S, et al. Identification of the novel activity-driven interaction between synaptotagmin 1 and presenilin 1 links calcium, synapse, and amyloid beta. BMC Biol. 2016;14:25 pubmed publisher
  • western blot; human; 1:3000; fig 4c
Kadir R, Harel T, Markus B, Perez Y, Bakhrat A, Cohen I, et al. ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size. PLoS Genet. 2016;12:e1005919 pubmed publisher
  • western blot; mouse; 1:10,000; fig 3f
Gruosso T, Mieulet V, Cardon M, Bourachot B, Kieffer Y, Devun F, et al. Chronic oxidative stress promotes H2AX protein degradation and enhances chemosensitivity in breast cancer patients. EMBO Mol Med. 2016;8:527-49 pubmed publisher
  • western blot; human; 1:5000; fig 2
Douanne T, Gavard J, Bidère N. The paracaspase MALT1 cleaves the LUBAC subunit HOIL1 during antigen receptor signaling. J Cell Sci. 2016;129:1775-80 pubmed publisher
  • western blot; human; 1:1000; fig s11
Nagao M, Ogata T, Sawada Y, Gotoh Y. Zbtb20 promotes astrocytogenesis during neocortical development. Nat Commun. 2016;7:11102 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Lee J, Kim H, Rho S, Lee S. eIF3f reduces tumor growth by directly interrupting clusterin with anti-apoptotic property in cancer cells. Oncotarget. 2016;7:18541-57 pubmed publisher
  • immunocytochemistry; mouse; 1:10,000; fig s6
Park S, Yun Y, Lim J, Kim M, Kim S, Kim J, et al. Stabilin-2 modulates the efficiency of myoblast fusion during myogenic differentiation and muscle regeneration. Nat Commun. 2016;7:10871 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
  • western blot; human; 1:1000; fig 3
Grampa V, Delous M, Zaidan M, Odye G, Thomas S, Elkhartoufi N, et al. Novel NEK8 Mutations Cause Severe Syndromic Renal Cystic Dysplasia through YAP Dysregulation. PLoS Genet. 2016;12:e1005894 pubmed publisher
  • immunoprecipitation; human; 1:5000; fig 4
Zhang X, Peng D, Xi Y, Yuan C, Sagum C, Klein B, et al. G9a-mediated methylation of ERα links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression. Nat Commun. 2016;7:10810 pubmed publisher
  • western blot; human; 1:3000; fig 3f
Choi W, de Poot S, Lee J, Kim J, Han D, Kim Y, et al. Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation. Nat Commun. 2016;7:10963 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 4e
  • western blot; human; 1:5000; fig 4g
Xu Q, Zhang Y, Wei Q, Huang Y, Hu J, Ling K. Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis. Nat Commun. 2016;7:10777 pubmed publisher
  • immunoprecipitation; human; fig s2e
  • immunocytochemistry; human; fig s2c
  • western blot; human; fig s2a
Oswald F, Rodriguez P, Giaimo B, Antonello Z, Mira L, Mittler G, et al. A phospho-dependent mechanism involving NCoR and KMT2D controls a permissive chromatin state at Notch target genes. Nucleic Acids Res. 2016;44:4703-20 pubmed publisher
  • western blot; human; fig 5f
Randles L, Anchoori R, Roden R, Walters K. The Proteasome Ubiquitin Receptor hRpn13 and Its Interacting Deubiquitinating Enzyme Uch37 Are Required for Proper Cell Cycle Progression. J Biol Chem. 2016;291:8773-83 pubmed publisher
  • immunoprecipitation; human; fig 4
  • western blot; human; fig 4
Lee S, Jeong A, Park J, Han S, Jang C, Kim K, et al. IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells. Cell Mol Life Sci. 2016;73:3375-86 pubmed publisher
  • immunoprecipitation; human; fig 1b
  • western blot; human; fig 1b
Hong J, Lee J, Chung I. Telomerase activates transcription of cyclin D1 gene through an interaction with NOL1. J Cell Sci. 2016;129:1566-79 pubmed publisher
  • western blot; human; 1:5000; fig 1
Vukićević T, Schulz M, Faust D, Klussmann E. The Trafficking of the Water Channel Aquaporin-2 in Renal Principal Cells-a Potential Target for Pharmacological Intervention in Cardiovascular Diseases. Front Pharmacol. 2016;7:23 pubmed publisher
  • western blot; human; fig 5
Castaño J, Morera C, Sesé B, Boue S, Bonet Costa C, Marti M, et al. SETD7 Regulates the Differentiation of Human Embryonic Stem Cells. PLoS ONE. 2016;11:e0149502 pubmed publisher
  • western blot; human; fig 1
Baietti M, Simíček M, Abbasi Asbagh L, Radaelli E, Lievens S, Crowther J, et al. OTUB1 triggers lung cancer development by inhibiting RAS monoubiquitination. EMBO Mol Med. 2016;8:288-303 pubmed publisher
  • western blot; human; fig 1
Kim J, He X, Orr B, Wutz G, Hill V, Peters J, et al. Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2. PLoS Genet. 2016;12:e1005865 pubmed publisher
  • immunoprecipitation; mouse; fig 4
  • western blot; mouse; 1:5000; fig 4
Tai D, Liu Y, Hsu W, Ma Y, Cheng S, Liu S, et al. MeCP2 SUMOylation rescues Mecp2-mutant-induced behavioural deficits in a mouse model of Rett syndrome. Nat Commun. 2016;7:10552 pubmed publisher
  • western blot; human; 1:5000; fig 5
Melero R, Hug N, López Perrote A, Yamashita A, Cáceres J, Llorca O. The RNA helicase DHX34 functions as a scaffold for SMG1-mediated UPF1 phosphorylation. Nat Commun. 2016;7:10585 pubmed publisher
  • immunoprecipitation; mouse
  • western blot; mouse
de Dieuleveult M, Yen K, Hmitou I, Depaux A, Boussouar F, Bou Dargham D, et al. Genome-wide nucleosome specificity and function of chromatin remodellers in ES cells. Nature. 2016;530:113-6 pubmed publisher
  • western blot; human; 1:1000; fig 3a
Martone J, Briganti F, Legnini I, Morlando M, Picillo E, Sthandier O, et al. The lack of the Celf2a splicing factor converts a Duchenne genotype into a Becker phenotype. Nat Commun. 2016;7:10488 pubmed publisher
  • western blot; human; fig s1
Lechtenberg B, Rajput A, Sanishvili R, Dobaczewska M, Ware C, Mace P, et al. Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase mechanism and regulation. Nature. 2016;529:546-50 pubmed publisher
  • western blot; mouse; fig s4b
Kibe T, Zimmermann M, de Lange T. TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres. Mol Cell. 2016;61:236-46 pubmed publisher
  • western blot; human; fig 1
  • western blot; mouse; fig 1
M L, P P, T K, M P, E S, J P, et al. Essential role of HDAC6 in the regulation of PD-L1 in melanoma. Mol Oncol. 2016;10:735-750 pubmed publisher
  • western blot; human; 1:4000; fig 4
Pan H, Guan D, Liu X, Li J, Wang L, Wu J, et al. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Res. 2016;26:190-205 pubmed publisher
  • immunoprecipitation; human; fig 3
  • western blot; human; fig 3
Zhang Q, Wan M, Shi J, Horita D, Miller L, Kute T, et al. Yin Yang 1 promotes mTORC2-mediated AKT phosphorylation. J Mol Cell Biol. 2016;8:232-43 pubmed publisher
  • immunoprecipitation; human; 1:20,000; fig 1
  • western blot; human; 1:20,000; fig 1
Zhang C, Liu J, Zhao Y, Yue X, Zhu Y, Wang X, et al. Glutaminase 2 is a novel negative regulator of small GTPase Rac1 and mediates p53 function in suppressing metastasis. elife. 2016;5:e10727 pubmed publisher
  • western blot; human; fig 3b
Li N, Dhar S, Chen T, Kan P, Wei Y, Kim J, et al. JARID1D Is a Suppressor and Prognostic Marker of Prostate Cancer Invasion and Metastasis. Cancer Res. 2016;76:831-43 pubmed publisher
  • western blot; human; fig 3
Kiel C, Benisty H, Lloréns Rico V, Serrano L. The yin-yang of kinase activation and unfolding explains the peculiarity of Val600 in the activation segment of BRAF. elife. 2016;5:e12814 pubmed publisher
  • immunocytochemistry; Leishmania amazonensis; 1:500; fig 1
Mittra B, Laranjeira Silva M, Perrone Bezerra de Menezes J, Jensen J, Michailowsky V, Andrews N. A Trypanosomatid Iron Transporter that Regulates Mitochondrial Function Is Required for Leishmania amazonensis Virulence. PLoS Pathog. 2016;12:e1005340 pubmed publisher
  • immunoprecipitation; human; fig s3b
Arimoto Matsuzaki K, Saito H, Takekawa M. TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat Commun. 2016;7:10252 pubmed publisher
  • western blot; human; fig 4
Long J, Giotis E, Moncorgé O, Frise R, Mistry B, James J, et al. Species difference in ANP32A underlies influenza A virus polymerase host restriction. Nature. 2016;529:101-4 pubmed publisher
  • ChIP-Seq; mouse; tbl  s3
Yasuma K, Yasunaga J, Takemoto K, Sugata K, Mitobe Y, Takenouchi N, et al. HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT). PLoS Pathog. 2016;12:e1005372 pubmed publisher
  • western blot; human; 1:8000; fig 4
Lee S, Frattini V, Bansal M, Castano A, Sherman D, Hutchinson K, et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016;529:172-7 pubmed publisher
  • immunohistochemistry; human; 1:50; fig e8
Shu S, Lin C, He H, Witwicki R, Tabassum D, Roberts J, et al. Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer. Nature. 2016;529:413-417 pubmed publisher
  • western blot; human; fig 1
Kim T, Jin F, Shin S, Oh S, Lightfoot S, Grande J, et al. Histone demethylase JMJD2A drives prostate tumorigenesis through transcription factor ETV1. J Clin Invest. 2016;126:706-20 pubmed publisher
  • immunoprecipitation; human; fig 4
Yu C, Liang J, Li J, Lee Y, Chang B, Su C, et al. Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins. PLoS Pathog. 2015;11:e1005350 pubmed publisher
  • immunoprecipitation; human; fig e1
Zhao D, Gish G, Braunschweig U, Li Y, Ni Z, Schmitges F, et al. SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination. Nature. 2016;529:48-53 pubmed publisher
  • immunocytochemistry; human; fig 6E
  • western blot; human; fig 1A
Ding X, Jiang W, Zhou P, Liu L, Wan X, Yuan X, et al. Mixed Lineage Leukemia 5 (MLL5) Protein Stability Is Cooperatively Regulated by O-GlcNac Transferase (OGT) and Ubiquitin Specific Protease 7 (USP7). PLoS ONE. 2015;10:e0145023 pubmed publisher
  • immunoprecipitation; mouse; fig 5
Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, et al. NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nat Immunol. 2016;17:250-8 pubmed publisher
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 1
Song H, Tao L, Chen C, Pan L, Hao J, Ni Y, et al. USP17-mediated deubiquitination and stabilization of HDAC2 in cigarette smoke extract-induced inflammation. Int J Clin Exp Pathol. 2015;8:10707-15 pubmed
  • immunoprecipitation; human; 1:1000; fig 3
  • western blot; human; 1:1000; fig 3
Bunda S, Burrell K, Heir P, Zeng L, Alamsahebpour A, Kano Y, et al. Inhibition of SHP2-mediated dephosphorylation of Ras suppresses oncogenesis. Nat Commun. 2015;6:8859 pubmed publisher
  • western blot; human; 1:1000; fig 4
Tollenaere M, Villumsen B, Blasius M, Nielsen J, Wagner S, Bartek J, et al. p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nat Commun. 2015;6:10075 pubmed publisher
  • western blot; human; 1:2000; fig s1a
Yan Y, Ollila S, Wong I, Vallenius T, Palvimo J, Vaahtomeri K, et al. SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling. Nat Commun. 2015;6:8979 pubmed publisher
  • immunoprecipitation; human; fig 2b
  • western blot; human; fig 2b
Stadhouders R, Cico A, Stephen T, Thongjuea S, Kolovos P, Baymaz H, et al. Control of developmentally primed erythroid genes by combinatorial co-repressor actions. Nat Commun. 2015;6:8893 pubmed publisher
  • immunoprecipitation; Drosophila melanogaster; 1:4000; fig 3
  • immunohistochemistry; Drosophila melanogaster; 1:500; fig 4
  • western blot; Drosophila melanogaster; 1:4000; fig 3
  • immunoprecipitation; African green monkey; 1:4000; fig 2
  • western blot; African green monkey; 1:4000; fig 2
Ukken F, Bruckner J, Weir K, Hope S, Sison S, Birschbach R, et al. BAR-SH3 sorting nexins are conserved interacting proteins of Nervous wreck that organize synapses and promote neurotransmission. J Cell Sci. 2016;129:166-77 pubmed publisher
  • immunoprecipitation; human; fig 2a
Vijayalingam S, Subramanian T, Zhao L, Chinnadurai G. The Cellular Protein Complex Associated with a Transforming Region of E1A Contains c-MYC. J Virol. 2016;90:1070-9 pubmed publisher
  • chromatin immunoprecipitation; human; fig e7
  • immunocytochemistry; human; fig e7
  • western blot; human; fig e7
Dou Z, Xu C, Donahue G, Shimi T, Pan J, Zhu J, et al. Autophagy mediates degradation of nuclear lamina. Nature. 2015;527:105-9 pubmed publisher
  • western blot; mouse; fig 4
Nady N, Gupta A, Ma Z, Swigut T, Koide A, Koide S, et al. ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation. elife. 2015;4:e10150 pubmed publisher
  • western blot; human; 1:2000
Zhang T, Xu Y, Liu Y, Ye Y. gp78 functions downstream of Hrd1 to promote degradation of misfolded proteins of the endoplasmic reticulum. Mol Biol Cell. 2015;26:4438-50 pubmed publisher
  • immunoprecipitation; human; fig 1c
  • western blot; human; 1:2000; fig 1c
Hu D, Gur M, Zhou Z, Gamper A, Hung M, Fujita N, et al. Interplay between arginine methylation and ubiquitylation regulates KLF4-mediated genome stability and carcinogenesis. Nat Commun. 2015;6:8419 pubmed publisher
  • immunoprecipitation; human; fig 5
Guo Y, Feng W, Sy S, Huen M. ATM-dependent Phosphorylation of the Fanconi Anemia Protein PALB2 Promotes the DNA Damage Response. J Biol Chem. 2015;290:27545-56 pubmed publisher
  • western blot; human; fig s7e
Pelish H, Liau B, Nitulescu I, Tangpeerachaikul A, Poss Z, Da Silva D, et al. Mediator kinase inhibition further activates super-enhancer-associated genes in AML. Nature. 2015;526:273-276 pubmed publisher
  • immunohistochemistry; mouse; fig 1d
Maqdasy S, El Hajjaji F, Baptissart M, Viennois E, Oumeddour A, Brugnon F, et al. Identification of the Functions of Liver X Receptor-β in Sertoli Cells Using a Targeted Expression-Rescue Model. Endocrinology. 2015;156:4545-57 pubmed publisher
  • western blot; human; 1:5000; fig 1
Rao X, Duan X, Mao W, Li X, Li Z, Li Q, et al. O-GlcNAcylation of G6PD promotes the pentose phosphate pathway and tumor growth. Nat Commun. 2015;6:8468 pubmed publisher
  • immunoprecipitation; human; fig 4e
Hilton B, Li Z, Musich P, Wang H, Cartwright B, SERRANO M, et al. ATR Plays a Direct Antiapoptotic Role at Mitochondria, which Is Regulated by Prolyl Isomerase Pin1. Mol Cell. 2015;60:35-46 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Anderson K, Russell A, Foletta V. NDRG2 promotes myoblast proliferation and caspase 3/7 activities during differentiation, and attenuates hydrogen peroxide - But not palmitate-induced toxicity. FEBS Open Bio. 2015;5:668-81 pubmed publisher
  • western blot; human; 1:1000; fig 1c
Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, et al. The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 2015;11:e1005503 pubmed publisher
  • immunoprecipitation; human; fig 1b
Zhu J, Sammons M, Donahue G, Dou Z, Vedadi M, Getlik M, et al. Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth. Nature. 2015;525:206-11 pubmed publisher
  • immunohistochemistry; Caenorhabditis elegans; 1:200; fig 5
Chen Y, Bharill S, Isacoff E, Chalfie M. Subunit composition of a DEG/ENaC mechanosensory channel of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2015;112:11690-5 pubmed publisher
  • immunocytochemistry; S. cerevisiae; 1:100; fig s1a
Jovičić A, Mertens J, Boeynaems S, Bogaert E, Chai N, Yamada S, et al. Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS. Nat Neurosci. 2015;18:1226-9 pubmed publisher
  • western blot; mouse; fig 3
Wang J, Zhang Y, Hou J, Qian X, Zhang H, Zhang Z, et al. Ube2s regulates Sox2 stability and mouse ES cell maintenance. Cell Death Differ. 2016;23:393-404 pubmed publisher
  • immunoprecipitation; human; fig 1e
  • western blot; human; fig 1e
Torres M, Pandita R, Kulak O, Kumar R, Formstecher E, Horikoshi N, et al. Role of the Exocyst Complex Component Sec6/8 in Genomic Stability. Mol Cell Biol. 2015;35:3633-45 pubmed publisher
  • western blot; human; fig 1
Steingruber M, Socher E, Hutterer C, Webel R, Bergbrede T, Lenac T, et al. The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain. Viruses. 2015;7:4582-601 pubmed publisher
  • immunoprecipitation; human; fig 3
Thandapani P, Song J, Gandin V, Cai Y, Rouleau S, Garant J, et al. Aven recognition of RNA G-quadruplexes regulates translation of the mixed lineage leukemia protooncogenes. elife. 2015;4: pubmed publisher
  • western blot; Drosophila melanogaster; 1:10,000
Chakraborty R, Li Y, Zhou L, Golic K. Corp Regulates P53 in Drosophila melanogaster via a Negative Feedback Loop. PLoS Genet. 2015;11:e1005400 pubmed publisher
  • western blot; human; fig 5f
Phan L, Chou P, Velazquez Torres G, Samudio I, Parreno K, Huang Y, et al. The cell cycle regulator 14-3-3σ opposes and reverses cancer metabolic reprogramming. Nat Commun. 2015;6:7530 pubmed publisher
  • western blot; human; 1:5000; fig 1
Chen S, Okada M, Nakato R, Izumi K, Bando M, Shirahige K. The Deubiquitinating Enzyme USP7 Regulates Androgen Receptor Activity by Modulating Its Binding to Chromatin. J Biol Chem. 2015;290:21713-23 pubmed publisher
  • immunoprecipitation; human; fig 2h
  • western blot; human; fig 2d
Shi J, Liu Y, Xu X, Zhang W, Yu T, Jia J, et al. Deubiquitinase USP47/UBP64E Regulates β-Catenin Ubiquitination and Degradation and Plays a Positive Role in Wnt Signaling. Mol Cell Biol. 2015;35:3301-11 pubmed publisher
  • western blot; rat; fig 6a
Bollimpelli V, Kondapi A. Enriched rat primary ventral mesencephalic neurons as an in-vitro culture model. Neuroreport. 2015;26:728-34 pubmed publisher
  • western blot; rat; fig 6a
Pedros C, Gaud G, Bernard I, Kassem S, Chabod M, Lagrange D, et al. An Epistatic Interaction between Themis1 and Vav1 Modulates Regulatory T Cell Function and Inflammatory Bowel Disease Development. J Immunol. 2015;195:1608-16 pubmed publisher
  • western blot; human; fig 6
Xu Q, Zhang Y, Wei Q, Huang Y, Li Y, Ling K, et al. BBS4 and BBS5 show functional redundancy in the BBSome to regulate the degradative sorting of ciliary sensory receptors. Sci Rep. 2015;5:11855 pubmed publisher
  • western blot; human; 1:1000; fig 5
Çelik H, Sajwan K, Selvanathan S, Marsh B, Pai A, Kont Y, et al. Ezrin Binds to DEAD-Box RNA Helicase DDX3 and Regulates Its Function and Protein Level. Mol Cell Biol. 2015;35:3145-62 pubmed publisher
  • immunohistochemistry; mouse; 1:6000; fig s1a
  • western blot; human; fig s1b
Tindi J, Chávez A, Cvejic S, Calvo Ochoa E, Castillo P, Jordan B. ANKS1B Gene Product AIDA-1 Controls Hippocampal Synaptic Transmission by Regulating GluN2B Subunit Localization. J Neurosci. 2015;35:8986-96 pubmed publisher
  • western blot; human; fig 1D
Amente S, Milazzo G, Sorrentino M, Ambrosio S, Di Palo G, Lania L, et al. Lysine-specific demethylase (LSD1/KDM1A) and MYCN cooperatively repress tumor suppressor genes in neuroblastoma. Oncotarget. 2015;6:14572-83 pubmed
  • immunohistochemistry; mouse; 1:6000; fig s1a
  • western blot; human; fig s1b
Omori Y, Chaya T, Yoshida S, Irie S, Tsujii T, Furukawa T. Identification of G Protein-Coupled Receptors (GPCRs) in Primary Cilia and Their Possible Involvement in Body Weight Control. PLoS ONE. 2015;10:e0128422 pubmed publisher
  • immunoprecipitation; human; fig 1
Lu H, Li Z, Zhang W, Schulze Gahmen U, Xue Y, Zhou Q. Gene target specificity of the Super Elongation Complex (SEC) family: how HIV-1 Tat employs selected SEC members to activate viral transcription. Nucleic Acids Res. 2015;43:5868-79 pubmed publisher
  • immunohistochemistry; human; 1:500; fig 2
Monaghan R, Barnes R, Fisher K, Andreou T, Rooney N, Poulin G, et al. A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity. Nat Cell Biol. 2015;17:782-92 pubmed publisher
  • immunoprecipitation; human
Grow E, Flynn R, CHAVEZ S, Bayless N, Wossidlo M, Wesche D, et al. Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells. Nature. 2015;522:221-5 pubmed publisher
  • immunoprecipitation; mouse; 1:5000; fig 7
  • western blot; mouse; 1:5000; fig 7
Stemig M, Astelford K, Emery A, Cho J, Allen B, Huang T, et al. Deletion of histone deacetylase 7 in osteoclasts decreases bone mass in mice by interactions with MITF. PLoS ONE. 2015;10:e0123843 pubmed publisher
  • ChIP-Seq; mouse; 1:2000; fig 5
Alexander J, Hota S, He D, Thomas S, Ho L, Pennacchio L, et al. Brg1 modulates enhancer activation in mesoderm lineage commitment. Development. 2015;142:1418-30 pubmed publisher
  • western blot; human; fig 6
Hendriks I, Treffers L, Verlaan de Vries M, Olsen J, Vertegaal A. SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. Cell Rep. 2015;10:1778-1791 pubmed publisher
  • western blot; human; 1:1000; fig 1,2,3,4,5,6,7
Desantis A, Bruno T, Catena V, De Nicola F, Goeman F, Iezzi S, et al. Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy. EMBO J. 2015;34:1214-30 pubmed publisher
  • western blot; human; 1:4000; fig s4
Conn S, Pillman K, Toubia J, Conn V, Salmanidis M, Phillips C, et al. The RNA binding protein quaking regulates formation of circRNAs. Cell. 2015;160:1125-34 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 1
  • western blot; human; 1:1000; fig 3
Kocyłowski M, Rey A, Stewart G, Halazonetis T. Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168. Cell Cycle. 2015;14:1748-58 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig s9
  • immunoprecipitation; human; 1:50; fig 4
Fillmore C, Xu C, Desai P, Berry J, Rowbotham S, Lin Y, et al. EZH2 inhibition sensitizes BRG1 and EGFR mutant lung tumours to TopoII inhibitors. Nature. 2015;520:239-42 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250; fig 3
Orr A, Hsiao E, Wang M, Ho K, Kim D, Wang X, et al. Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory. Nat Neurosci. 2015;18:423-34 pubmed publisher
  • western blot; human; 1:2000; fig 2
Xu J, Eriksson S, Cebula M, Sandalova T, Hedström E, Pader I, et al. The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death. Cell Death Dis. 2015;6:e1616 pubmed publisher
  • western blot; mouse
Kanazawa I, Canaff L, Abi Rafeh J, Angrula A, Li J, Riddle R, et al. Osteoblast menin regulates bone mass in vivo. J Biol Chem. 2015;290:3910-24 pubmed publisher
  • immunoprecipitation; mouse; 1:500-1:1000; fig 7
Imajo M, Ebisuya M, Nishida E. Dual role of YAP and TAZ in renewal of the intestinal epithelium. Nat Cell Biol. 2015;17:7-19 pubmed publisher
  • western blot; human
Ling X, Xu C, Fan C, Zhong K, Li F, Wang X. FL118 induces p53-dependent senescence in colorectal cancer cells by promoting degradation of MdmX. Cancer Res. 2014;74:7487-97 pubmed publisher
  • western blot; human; fig 4
Zou J, Zhang D, Qin G, Chen X, Wang H, Zhang D. BRCA1 and FancJ cooperatively promote interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1. Cell Cycle. 2014;13:3685-97 pubmed publisher
  • immunoprecipitation; mouse; fig 4
  • western blot; mouse; fig 4
Bittencourt D, Lee B, Gao L, Gerke D, Stallcup M. Role of distinct surfaces of the G9a ankyrin repeat domain in histone and DNA methylation during embryonic stem cell self-renewal and differentiation. Epigenetics Chromatin. 2014;7:27 pubmed publisher
  • immunoprecipitation; human; fig 8
  • immunocytochemistry; human; 1:200; fig 7
  • western blot; human; 1:3000; fig 8
Liu Y, Luo S, He S, Zhang M, Wang P, Li C, et al. Tetherin restricts HSV-2 release and is counteracted by multiple viral glycoproteins. Virology. 2015;475:96-109 pubmed publisher
  • immunocytochemistry; human; 1:5000
  • western blot; human; 1:1000
Kitai Y, Takeuchi O, Kawasaki T, Ori D, Sueyoshi T, Murase M, et al. Negative regulation of melanoma differentiation-associated gene 5 (MDA5)-dependent antiviral innate immune responses by Arf-like protein 5B. J Biol Chem. 2015;290:1269-80 pubmed publisher
  • immunoprecipitation; human; fig 1
  • western blot; human; 1:1000; fig 1
Ma B, Chen Y, Chen L, Cheng H, Mu C, Li J, et al. Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase. Nat Cell Biol. 2015;17:95-103 pubmed publisher
  • western blot; mouse; 1:3000
Bongers K, Fox D, Kunkel S, Stebounova L, Murry D, Pufall M, et al. Spermine oxidase maintains basal skeletal muscle gene expression and fiber size and is strongly repressed by conditions that cause skeletal muscle atrophy. Am J Physiol Endocrinol Metab. 2015;308:E144-58 pubmed publisher
  • western blot; mouse; 1:500; fig 7
Huang L, Carney J, Cardona D, Counter C. Decreased tumorigenesis in mice with a Kras point mutation at C118. Nat Commun. 2014;5:5410 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Eifler T, Shao W, Bartholomeeusen K, Fujinaga K, Jäger S, Johnson J, et al. Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts. Mol Cell Biol. 2015;35:468-78 pubmed publisher
  • immunoprecipitation; human; 1:5000; fig s8
Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 4
  • western blot; mouse; fig 4
Shi X, Zhang Z, Zhan X, Cao M, Satoh T, Akira S, et al. An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth. Nat Commun. 2014;5:5425 pubmed publisher
  • immunoprecipitation; human
  • western blot; human; fig 4
Wang Y, Yang Y, Wu S, Pan S, Zhou C, Ma Y, et al. p32 is a novel target for viral protein ICP34.5 of herpes simplex virus type 1 and facilitates viral nuclear egress. J Biol Chem. 2014;289:35795-805 pubmed publisher
  • immunoprecipitation; mouse; fig 8
  • western blot; mouse; fig 8
Liang N, Zhang C, Dill P, Panasyuk G, Pion D, Koka V, et al. Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex. J Exp Med. 2014;211:2249-63 pubmed publisher
  • immunoprecipitation; human
Tsai K, Chan L, Gibeault R, Conn K, Dheekollu J, Domsic J, et al. Viral reprogramming of the Daxx histone H3.3 chaperone during early Epstein-Barr virus infection. J Virol. 2014;88:14350-63 pubmed publisher
  • immunoprecipitation; human; 1:100
Liang Y, Eng W, Colquhoun D, Dinglasan R, Graham D, Mahal L. Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J Biol Chem. 2014;289:32526-37 pubmed publisher
  • western blot; human; loading ...; fig 6a
Merabova N, Sariyer I, Saribas A, Knezevic T, Gordon J, Turco M, et al. WW domain of BAG3 is required for the induction of autophagy in glioma cells. J Cell Physiol. 2015;230:831-41 pubmed publisher
  • western blot; mouse; fig 2
Han M, Hu Z, Chen C, Chen Y, Gucek M, Li Z, et al. Dysbindin-associated proteome in the p2 synaptosome fraction of mouse brain. J Proteome Res. 2014;13:4567-80 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
de Souza E, Meirelles G, Godoy B, Perez A, Smetana J, Doxsey S, et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res. 2014;13:4074-90 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Putker M, Vos H, van Dorenmalen K, de Ruiter H, Duran A, Snel B, et al. Evolutionary acquisition of cysteines determines FOXO paralog-specific redox signaling. Antioxid Redox Signal. 2015;22:15-28 pubmed publisher
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse
  • western blot; mouse
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Ro S, Semple I, Park H, Park H, Park H, Kim M, et al. Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of p62/sequestosome-1. FEBS J. 2014;281:3816-27 pubmed publisher
  • western blot; human
Lo Sasso G, Ryu D, Mouchiroud L, Fernando S, Anderson C, Katsyuba E, et al. Loss of Sirt1 function improves intestinal anti-bacterial defense and protects from colitis-induced colorectal cancer. PLoS ONE. 2014;9:e102495 pubmed publisher
  • immunoprecipitation; human
Zhang P, Gao K, Tang Y, Jin X, An J, Yu H, et al. Destruction of DDIT3/CHOP protein by wild-type SPOP but not prostate cancer-associated mutants. Hum Mutat. 2014;35:1142-51 pubmed publisher
  • immunocytochemistry; human
O Rourke B, Gomez Ferreria M, Berk R, Hackl A, Nicholas M, O Rourke S, et al. Cep192 controls the balance of centrosome and non-centrosomal microtubules during interphase. PLoS ONE. 2014;9:e101001 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Choi Y, Kim Y, Jeong H, Jin Y, Yeo C, Lee K. Akt enhances Runx2 protein stability by regulating Smurf2 function during osteoblast differentiation. FEBS J. 2014;281:3656-66 pubmed publisher
  • western blot; human; fig 2
Hayashi A, Suenaga N, Shiomi Y, Nishitani H. PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells. Methods Mol Biol. 2014;1170:367-82 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 5e
  • western blot; mouse; 1:1000; fig s5b
Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge N, et al. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Rep. 2014;7:1456-1470 pubmed publisher
  • western blot; human
Lu H, Fang E, Sykora P, Kulikowicz T, Zhang Y, Becker K, et al. Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice. Cell Death Dis. 2014;5:e1226 pubmed publisher
  • immunocytochemistry; human; 1:500
Hans F, Fiesel F, Strong J, J ckel S, Rasse T, Geisler S, et al. UBE2E ubiquitin-conjugating enzymes and ubiquitin isopeptidase Y regulate TDP-43 protein ubiquitination. J Biol Chem. 2014;289:19164-79 pubmed publisher
  • western blot; Xenopus laevis; 0.5 ug/ml
Kang Q, Srividhya J, Ipe J, Pomerening J. Evidence toward a dual phosphatase mechanism that restricts Aurora A (Thr-295) phosphorylation during the early embryonic cell cycle. J Biol Chem. 2014;289:17480-96 pubmed publisher
  • flow cytometry; human; 1:50000
  • immunoprecipitation; human; 1:50000
  • western blot; human; 1:50000
van de Weijer M, Bassik M, Luteijn R, Voorburg C, Lohuis M, Kremmer E, et al. A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation. Nat Commun. 2014;5:3832 pubmed publisher
  • immunocytochemistry; human
Vidi P, Liu J, Salles D, Jayaraman S, Dorfman G, Gray M, et al. NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks. Nucleic Acids Res. 2014;42:6365-79 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Cipriano R, Miskimen K, Bryson B, Foy C, Bartel C, Jackson M. Conserved oncogenic behavior of the FAM83 family regulates MAPK signaling in human cancer. Mol Cancer Res. 2014;12:1156-65 pubmed publisher
  • immunocytochemistry; human
Kerdivel G, Boudot A, Habauzit D, Percevault F, Demay F, Pakdel F, et al. Activation of the MKL1/actin signaling pathway induces hormonal escape in estrogen-responsive breast cancer cell lines. Mol Cell Endocrinol. 2014;390:34-44 pubmed publisher
  • western blot; mouse; 1:5000
Okuda H, Tatsumi K, Horii Hayashi N, Morita S, Okuda Yamamoto A, Imaizumi K, et al. OASIS regulates chondroitin 6-O-sulfotransferase 1 gene transcription in the injured adult mouse cerebral cortex. J Neurochem. 2014;130:612-25 pubmed publisher
  • western blot; human
Havel J, Li Z, Cheng D, Peng J, Fu H. Nuclear PRAS40 couples the Akt/mTORC1 signaling axis to the RPL11-HDM2-p53 nucleolar stress response pathway. Oncogene. 2015;34:1487-98 pubmed publisher
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse
  • western blot; mouse
Adeyemi R, Fuller M, Pintel D. Efficient parvovirus replication requires CRL4Cdt2-targeted depletion of p21 to prevent its inhibitory interaction with PCNA. PLoS Pathog. 2014;10:e1004055 pubmed publisher
  • immunocytochemistry; mouse
Vreeland A, Yu S, Levi L, de Barros Rossetto D, Noy N. Transcript stabilization by the RNA-binding protein HuR is regulated by cellular retinoic acid-binding protein 2. Mol Cell Biol. 2014;34:2135-46 pubmed publisher
  • chromatin immunoprecipitation; human
  • immunoprecipitation; human
  • immunohistochemistry; human; 1:1000
  • western blot; human; 1:1000
Rennoll S, Scott S, Yochum G. Targeted repression of AXIN2 and MYC gene expression using designer TALEs. Biochem Biophys Res Commun. 2014;446:1120-5 pubmed publisher
  • immunoprecipitation; mouse
  • western blot; mouse
Huang Y, Liu H, Li S, Tang Y, Wei B, Yu H, et al. MAVS-MKK7-JNK2 defines a novel apoptotic signaling pathway during viral infection. PLoS Pathog. 2014;10:e1004020 pubmed publisher
  • western blot; human
Wang Q, Shen B, Chen L, Zheng P, Feng H, Hao Q, et al. Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis. Oncogene. 2015;34:1006-18 pubmed publisher
  • immunocytochemistry; human; 1:400
Justa Schuch D, Moller U, Geiss Friedlander R. The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus. Cell Mol Life Sci. 2014;71:3611-26 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Matthess Y, Raab M, Knecht R, Becker S, Strebhardt K. Sequential Cdk1 and Plk1 phosphorylation of caspase-8 triggers apoptotic cell death during mitosis. Mol Oncol. 2014;8:596-608 pubmed publisher
  • immunocytochemistry; human
Salvi M, Raiborg C, Hanson P, Campsteijn C, Stenmark H, Pinna L. CK2 involvement in ESCRT-III complex phosphorylation. Arch Biochem Biophys. 2014;545:83-91 pubmed publisher
  • western blot; human; fig 1
Aida K, Hayashi H, Inamura K, Mizuno T, Sugiyama Y. Differential roles of ubiquitination in the degradation mechanism of cell surface-resident bile salt export pump and multidrug resistance-associated protein 2. Mol Pharmacol. 2014;85:482-91 pubmed publisher
  • western blot; human; 1:1000
Annibaldi A, Heulot M, Martinou J, Widmann C. TAT-RasGAP317-326-mediated tumor cell death sensitization can occur independently of Bax and Bak. Apoptosis. 2014;19:719-33 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Zhang W, Ji W, Liu X, Ouyang G, Xiao W. ELL inhibits E2F1 transcriptional activity by enhancing E2F1 deacetylation via recruitment of histone deacetylase 1. Mol Cell Biol. 2014;34:765-75 pubmed publisher
  • immunocytochemistry; African green monkey
  • western blot; mouse
Brot S, Auger C, Bentata R, Rogemond V, Ménigoz S, Chounlamountri N, et al. Collapsin response mediator protein 5 (CRMP5) induces mitophagy, thereby regulating mitochondrion numbers in dendrites. J Biol Chem. 2014;289:2261-76 pubmed publisher
  • immunohistochemistry - frozen section; Xenopus laevis
Chu C, Gerstenzang E, Ossipova O, Sokol S. Lulu regulates Shroom-induced apical constriction during neural tube closure. PLoS ONE. 2013;8:e81854 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Kriz V, Pospichalova V, Masek J, Kilander M, Slavík J, Tanneberger K, et al. ?-arrestin promotes Wnt-induced low density lipoprotein receptor-related protein 6 (Lrp6) phosphorylation via increased membrane recruitment of Amer1 protein. J Biol Chem. 2014;289:1128-41 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human; fig 2
Liu X, Xiao W, Wang X, Li Y, Han J, Li Y. The p38-interacting protein (p38IP) regulates G2/M progression by promoting ?-tubulin acetylation via inhibiting ubiquitination-induced degradation of the acetyltransferase GCN5. J Biol Chem. 2013;288:36648-61 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
Sharma N, Zhu Q, Wani G, He J, Wang Q, Wani A. USP3 counteracts RNF168 via deubiquitinating H2A and ?H2AX at lysine 13 and 15. Cell Cycle. 2014;13:106-14 pubmed publisher
  • western blot; mouse; 1:2000
Evans C, Cook S, Coleman M, Gilley J. MEK inhibitor U0126 reverses protection of axons from Wallerian degeneration independently of MEK-ERK signaling. PLoS ONE. 2013;8:e76505 pubmed publisher
  • immunocytochemistry; human; fig 2b
Hashizume C, Kobayashi A, Wong R. Down-modulation of nucleoporin RanBP2/Nup358 impaired chromosomal alignment and induced mitotic catastrophe. Cell Death Dis. 2013;4:e854 pubmed publisher
  • western blot; fission yeast
DeGennaro C, Alver B, Marguerat S, Stepanova E, Davis C, Bähler J, et al. Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast. Mol Cell Biol. 2013;33:4779-92 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Berndt A, Wilkinson K, Heimann M, Bishop P, Henley J. In vivo characterization of the properties of SUMO1-specific monobodies. Biochem J. 2013;456:385-95 pubmed publisher
  • immunoprecipitation; human
  • western blot; human; fig 3
Verma S, Cai Q, Kreider E, Lu J, Robertson E. Comprehensive analysis of LANA interacting proteins essential for viral genome tethering and persistence. PLoS ONE. 2013;8:e74662 pubmed publisher
  • western blot; human
Ahmed S, Prigent S. A nuclear export signal and oxidative stress regulate ShcD subcellular localisation: a potential role for ShcD in the nucleus. Cell Signal. 2014;26:32-40 pubmed publisher
  • immunoprecipitation; human
Shats I, Gatza M, Liu B, Angus S, You L, Nevins J. FOXO transcription factors control E2F1 transcriptional specificity and apoptotic function. Cancer Res. 2013;73:6056-67 pubmed publisher
  • immunocytochemistry; mouse; 1:1000
  • immunocytochemistry; human; 1:1000
Wang C, Low W, Liu A, Wang B. Centrosomal protein DZIP1 regulates Hedgehog signaling by promoting cytoplasmic retention of transcription factor GLI3 and affecting ciliogenesis. J Biol Chem. 2013;288:29518-29 pubmed publisher
  • western blot; human
Kumar M, Allison D, Baranova N, Wamsley J, Katz A, Bekiranov S, et al. NF-?B regulates mesenchymal transition for the induction of non-small cell lung cancer initiating cells. PLoS ONE. 2013;8:e68597 pubmed publisher
  • immunoprecipitation; human; 1:200-1:500; fig 1
Gurkar A, Chu K, Raj L, Bouley R, Lee S, Kim Y, et al. Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress. Nat Commun. 2013;4:2189 pubmed publisher
  • western blot; human
Sy S, Jiang J, O W, Deng Y, Huen M. The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks. Nucleic Acids Res. 2013;41:8572-80 pubmed publisher
  • immunocytochemistry; human
Chandra S, Priyadarshini R, Madhavan V, Tikoo S, Hussain M, Mudgal R, et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci. 2013;126:3782-95 pubmed publisher
  • immunoprecipitation; human
Wei Q, Li J, Liu T, Tong X, Ye X. Phosphorylation of minichromosome maintenance protein 7 (MCM7) by cyclin/cyclin-dependent kinase affects its function in cell cycle regulation. J Biol Chem. 2013;288:19715-25 pubmed publisher
  • western blot; dogs
Boehlke C, Kotsis F, Buchholz B, Powelske C, Eckardt K, Walz G, et al. Kif3a guides microtubular dynamics, migration and lumen formation of MDCK cells. PLoS ONE. 2013;8:e62165 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Kubota S, Fukumoto Y, Aoyama K, Ishibashi K, Yuki R, Morinaga T, et al. Phosphorylation of KRAB-associated protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by nuclear tyrosine kinases inhibits the association of KAP1 and heterochromatin protein 1? (HP1?) with heterochromatin. J Biol Chem. 2013;288:17871-83 pubmed publisher
  • chromatin immunoprecipitation; human
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Zhou P, Wang Z, Yuan X, Zhou C, Liu L, Wan X, et al. Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1). J Biol Chem. 2013;288:17532-43 pubmed publisher
  • western blot; human
Yasuda Inoue M, Kuroki M, Ariumi Y. Distinct DDX DEAD-box RNA helicases cooperate to modulate the HIV-1 Rev function. Biochem Biophys Res Commun. 2013;434:803-8 pubmed publisher
  • western blot; human; fig 4
Tinnikov A, Samuels H. A novel cell lysis approach reveals that caspase-2 rapidly translocates from the nucleus to the cytoplasm in response to apoptotic stimuli. PLoS ONE. 2013;8:e61085 pubmed publisher
  • western blot; human
Fu H, Sohail A, Valiathan R, Wasinski B, Kumarasiri M, Mahasenan K, et al. Shedding of discoidin domain receptor 1 by membrane-type matrix metalloproteinases. J Biol Chem. 2013;288:12114-29 pubmed publisher
  • western blot; rat
Murata Y, Constantine Paton M. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway. J Neurosci. 2013;33:5040-52 pubmed publisher
  • immunoprecipitation; mouse
Zhang J, Twelvetrees A, Lazarus J, Blasier K, Yao X, Inamdar N, et al. Establishing a novel knock-in mouse line for studying neuronal cytoplasmic dynein under normal and pathologic conditions. Cytoskeleton (Hoboken). 2013;70:215-27 pubmed publisher
  • immunocytochemistry; chicken; 1:500
Wang Y, Dantas T, Lalor P, Dockery P, Morrison C. Promoter hijack reveals pericentrin functions in mitosis and the DNA damage response. Cell Cycle. 2013;12:635-46 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Dettmer U, Newman A, Luth E, Bartels T, Selkoe D. In vivo cross-linking reveals principally oligomeric forms of ?-synuclein and ?-synuclein in neurons and non-neural cells. J Biol Chem. 2013;288:6371-85 pubmed publisher
  • western blot; chicken
  • immunoprecipitation; human
  • western blot; human; fig s10
Moreno Garcia M, Sommer K, Rincón Arano H, Brault M, Ninomiya Tsuji J, Matesic L, et al. Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-?B activation. Mol Cell Biol. 2013;33:1149-63 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Deguchi K, Nagamatsu G, Miyachi H, Kato Y, Morita S, Kimura H, et al. Posttranscriptional regulation of histone lysine methyltransferase GLP in embryonic male mouse germ cells. Biol Reprod. 2013;88:36 pubmed publisher
  • western blot; human
Morlando M, Dini Modigliani S, Torrelli G, Rosa A, Di Carlo V, Caffarelli E, et al. FUS stimulates microRNA biogenesis by facilitating co-transcriptional Drosha recruitment. EMBO J. 2012;31:4502-10 pubmed publisher
  • immunocytochemistry; African green monkey; 1:2000
  • western blot; African green monkey; 1:4000
Varco Merth B, Mirza K, Alzhanov D, Chia D, Rotwein P. Biochemical characterization of diverse Stat5b-binding enhancers that mediate growth hormone-activated insulin-like growth factor-I gene transcription. PLoS ONE. 2012;7:e50278 pubmed publisher
  • immunoprecipitation; human
  • immunoprecipitation; mouse
Wang C, Kao Y, Lai P, Chen W, Chung B. Steroidogenic factor 1 (NR5A1) maintains centrosome homeostasis in steroidogenic cells by restricting centrosomal DNA-dependent protein kinase activation. Mol Cell Biol. 2013;33:476-84 pubmed publisher
  • western blot; human
Journo C, Bonnet A, Favre Bonvin A, Turpin J, Vinera J, C t E, et al. Human T cell leukemia virus type 2 tax-mediated NF-?B activation involves a mechanism independent of Tax conjugation to ubiquitin and SUMO. J Virol. 2013;87:1123-36 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Katoh H, Okamoto T, Fukuhara T, Kambara H, Morita E, Mori Y, et al. Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation. J Virol. 2013;87:489-502 pubmed publisher
  • immunocytochemistry; mouse
Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro R. Functional dissection of Pax3 in paraxial mesoderm development and myogenesis. Stem Cells. 2013;31:59-70 pubmed publisher
  • immunoprecipitation; human
Yeung F, Chung E, Guess M, Bell M, Leinwand L. Myh7b/miR-499 gene expression is transcriptionally regulated by MRFs and Eos. Nucleic Acids Res. 2012;40:7303-18 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Zhou F, Zhang X, van Dam H, Ten Dijke P, Huang H, Zhang L. Ubiquitin-specific protease 4 mitigates Toll-like/interleukin-1 receptor signaling and regulates innate immune activation. J Biol Chem. 2012;287:11002-10 pubmed publisher
  • western blot; human
  • western blot; mouse
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product information
Catalog Number :
F1804
Product Name :
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
Product Type :
ELISAS
Product Group :
Immunoassay Platform Solutions
Product Description :
1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA