product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Dinitrophenyl antibody produced in mouse
catalog :
D8406
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SPE-7
reactivity :
human
application :
western blot
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; fig 5
Ambrosi G, Ghezzi C, Sepe S, Milanese C, Payan Gomez C, Bombardieri C, et al. Bioenergetic and proteolytic defects in fibroblasts from patients with sporadic Parkinson's disease. Biochim Biophys Acta. 2014;1842:1385-94 pubmed publisher
  • western blot; human
Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
Strattan E, Palaniyandi S, Kumari R, Du J, Hakim N, Huang T, et al. Mast Cells Are Mediators of Fibrosis and Effector Cell Recruitment in Dermal Chronic Graft-vs.-Host Disease. Front Immunol. 2019;10:2470 pubmed publisher
Yu J, Liu Z, Liang Y, Luo F, Zhang J, Tian C, et al. Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells. Nat Commun. 2019;10:4664 pubmed publisher
Njomen E, Tepe J. Regulation of Autophagic Flux by the 20S Proteasome. Cell Chem Biol. 2019;26:1283-1294.e5 pubmed publisher
Angelin A, Bog U, Kumar R, Niemeyer C, Hirtz M. Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns. Polymers (Basel). 2019;11: pubmed publisher
Haselmayer P, Camps M, Liu Bujalski L, Nguyen N, Morandi F, Head J, et al. Efficacy and Pharmacodynamic Modeling of the BTK Inhibitor Evobrutinib in Autoimmune Disease Models. J Immunol. 2019;202:2888-2906 pubmed publisher
Klebanovych A, Sladkova V, Sulimenko T, Vosecká V, Capek M, Dráberová E, et al. Regulation of Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells by Protein Tyrosine Phosphatase SHP-1. Cells. 2019;8: pubmed publisher
Zhang X, Huang Q, Wang X, Deng Z, Li J, Yan X, et al. Dietary cholesterol is essential to mast cell activation and associated obesity and diabetes in mice. Biochim Biophys Acta Mol Basis Dis. 2019;1865:1690-1700 pubmed publisher
Kim M, Im D. Suppressive effects of type I angiotensin receptor antagonists, candesartan and irbesartan on allergic asthma. Eur J Pharmacol. 2019;852:25-33 pubmed publisher
Kawamoto Y, Kondo H, Hasegawa M, Kurimoto C, Ishii Y, Kato C, et al. Inhibition of mast cell degranulation by melanin. Biochem Pharmacol. 2019;163:178-193 pubmed publisher
Park S, Im D. Blockage of sphingosine-1-phosphate receptor 2 attenuates allergic asthma in mice. Br J Pharmacol. 2019;176:938-949 pubmed publisher
Jin F, Li X, Deng Y, Timilshina M, Huang B, Kim D, et al. The orphan nuclear receptor NR4A1 promotes FcεRI-stimulated mast cell activation and anaphylaxis by counteracting the inhibitory LKB1/AMPK axis. Allergy. 2019;74:1145-1156 pubmed publisher
Park Y, Kim H, Kim H, Nam S, Lee D, Lee M, et al. An Anti-Cancer Drug Candidate CYC116 Suppresses Type I Hypersensitive Immune Responses through the Inhibition of Fyn Kinase in Mast Cells. Biomol Ther (Seoul). 2019;27:311-317 pubmed publisher
Schrand B, Clark E, Levay A, Capote A, Martínez O, Brenneman R, et al. Hapten-mediated recruitment of polyclonal antibodies to tumors engenders antitumor immunity. Nat Commun. 2018;9:3348 pubmed publisher
Lianto P, Ogutu F, Zhang Y, He F, Che H. Inhibitory effects of quail egg on mast cells degranulation by suppressing PAR2-mediated MAPK and NF-kB activation. Food Nutr Res. 2018;62: pubmed publisher
Hu Frisk J, Kjellen L, Melo F, Ohrvik H, Pejler G. Mitogen-Activated Protein Kinase Signaling Regulates Proteoglycan Composition of Mast Cell Secretory Granules. Front Immunol. 2018;9:1670 pubmed publisher
Yang Y, Kong B, Jung Y, Park J, Oh J, Hwang J, et al. Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor-Derived Peptides for Regulation of Mast Cell Degranulation. Front Immunol. 2018;9:725 pubmed publisher
Huang J, Su M, Lee B, Kim M, Jung J, Im D. Suppressive Effect of 4-Hydroxy-2-(4-Hydroxyphenethyl) Isoindoline-1,3-Dione on Ovalbumin-Induced Allergic Asthma. Biomol Ther (Seoul). 2018;26:539-545 pubmed publisher
Li Y, Yu H, Jin Y, Li M, Qu C. Verbascoside Alleviates Atopic Dermatitis-Like Symptoms in Mice via Its Potent Anti-Inflammatory Effect. Int Arch Allergy Immunol. 2018;175:220-230 pubmed publisher
Cournot M, Burillo E, Saulnier P, Planesse C, Gand E, Rehman M, et al. Circulating Concentrations of Redox Biomarkers Do Not Improve the Prediction of Adverse Cardiovascular Events in Patients With Type 2 Diabetes Mellitus. J Am Heart Assoc. 2018;7: pubmed publisher
Lee B, Park S, Nam S, Kang S, Hwang J, Lee S, et al. Anti-allergic effects of sesquiterpene lactones from Saussurea costus (Falc.) Lipsch. determined using in vivo and in vitro experiments. J Ethnopharmacol. 2018;213:256-261 pubmed publisher
Phong B, Kane L. Mast cell activation is enhanced by Tim1:Tim4 interaction but not by Tim-1 antibodies. F1000Res. 2016;5:251 pubmed publisher
La Fata G, van Vliet N, Barnhoorn S, Brandt R, Etheve S, Chenal E, et al. Vitamin E Supplementation Reduces Cellular Loss in the Brain of a Premature Aging Mouse Model. J Prev Alzheimers Dis. 2017;4:226-235 pubmed publisher
Yang Y, Xiong D, Pipathsouk A, Weiner O, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Dev Cell. 2017;43:507-521.e4 pubmed publisher
Klein O, Roded A, Zur N, Azouz N, Pasternak O, Hirschberg K, et al. Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis. Sci Rep. 2017;7:15315 pubmed publisher
Drube S, Grimlowski R, Deppermann C, Fröbel J, Kraft F, Andreas N, et al. The Neurobeachin-like 2 Protein Regulates Mast Cell Homeostasis. J Immunol. 2017;199:2948-2957 pubmed publisher
Han S, Sun L, He F, Che H. Anti-allergic activity of glycyrrhizic acid on IgE-mediated allergic reaction by regulation of allergy-related immune cells. Sci Rep. 2017;7:7222 pubmed publisher
Li D, Wu L. Coumarins from the roots of Angelica dahurica cause anti-allergic inflammation. Exp Ther Med. 2017;14:874-880 pubmed publisher
Motzik A, Amir E, Erlich T, Wang J, Kim B, Han J, et al. Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells. Oncogene. 2017;36:4732-4738 pubmed publisher
Honjoh C, Chihara K, Yoshiki H, Yamauchi S, Takeuchi K, Kato Y, et al. Association of C-Type Lectin Mincle with FcεRIβγ Subunits Leads to Functional Activation of RBL-2H3 Cells through Syk. Sci Rep. 2017;7:46064 pubmed publisher
Ma Y, Yang M, He Z, Fan M, Huang M, Teng F, et al. Upregulation of heme oxygenase-1 in Kupffer cells blocks mast cell degranulation and inhibits dendritic cell migration in vitro. Mol Med Rep. 2017;15:3796-3802 pubmed publisher
Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, et al. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin. Free Radic Biol Med. 2017;102:133-148 pubmed publisher
Wang C, Chen H, Zhu W, Xu Y, Liu M, Zhu L, et al. Nicotine Accelerates Atherosclerosis in Apolipoprotein E-Deficient Mice by Activating ?7 Nicotinic Acetylcholine Receptor on Mast Cells. Arterioscler Thromb Vasc Biol. 2017;37:53-65 pubmed publisher
Qin S, Wang X, Wu H, Xiao P, Cheng H, Zhang X, et al. Cell-based phenotypic screening of mast cell degranulation unveils kinetic perturbations of agents targeting phosphorylation. Sci Rep. 2016;6:31320 pubmed publisher
Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner R, Wang A, et al. Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion. EMBO J. 2016;35:1810-21 pubmed publisher
Lee A, Kang S, Park S, Huang J, Im D. Anti-Allergic Effect of Oroxylin A from Oroxylum indicum Using in vivo and in vitro Experiments. Biomol Ther (Seoul). 2016;24:283-90 pubmed publisher
Baek S, Nam I, Kwak H, Kim K, Lee S. Cellular Anti-Melanogenic Effects of a Euryale ferox Seed Extract Ethyl Acetate Fraction via the Lysosomal Degradation Machinery. Int J Mol Sci. 2015;16:9217-35 pubmed publisher
Li Y, Qi X, Liu B, Huang H. The STAT5-GATA2 pathway is critical in basophil and mast cell differentiation and maintenance. J Immunol. 2015;194:4328-38 pubmed publisher
Lee K, Kang S, Noh M, Park S, Kim J, Chung H, et al. Therapeutic effects of s-petasin on disease models of asthma and peritonitis. Biomol Ther (Seoul). 2015;23:45-52 pubmed publisher
Launay N, Aguado C, Fourcade S, Ruiz M, Grau L, Riera J, et al. Autophagy induction halts axonal degeneration in a mouse model of X-adrenoleukodystrophy. Acta Neuropathol. 2015;129:399-415 pubmed publisher
Schwartz S, Yan Q, Telmer C, Lidke K, Bruchez M, Lidke D. Fluorogen-activating proteins provide tunable labeling densities for tracking FcεRI independent of IgE. ACS Chem Biol. 2015;10:539-46 pubmed publisher
Zhou Q, Lee G, Brady J, Datta S, Katan M, Sheikh A, et al. A hypermorphic missense mutation in PLCG2, encoding phospholipase Cγ2, causes a dominantly inherited autoinflammatory disease with immunodeficiency. Am J Hum Genet. 2012;91:713-20 pubmed publisher
Palmer R, Hutchinson L, Burpee B, Tupper E, Pelletier J, Kormendy Z, et al. Antibacterial agent triclosan suppresses RBL-2H3 mast cell function. Toxicol Appl Pharmacol. 2012;258:99-108 pubmed publisher
Qi X, Nishida J, Chaves L, Ohmori K, Huang H. CCAAT/enhancer-binding protein alpha (C/EBPalpha) is critical for interleukin-4 expression in response to FcepsilonRI receptor cross-linking. J Biol Chem. 2011;286:16063-73 pubmed publisher
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Fifadara N, Beer F, Ono S, Ono S. Interaction between activated chemokine receptor 1 and FcepsilonRI at membrane rafts promotes communication and F-actin-rich cytoneme extensions between mast cells. Int Immunol. 2010;22:113-28 pubmed publisher
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Jasani B, Thomas N, Navabi H, Millar D, Newman G, Gee J, et al. Dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure. A 10 year review of its principle reagents and applications. J Immunol Methods. 1992;150:193-8 pubmed
product information
Product No. :
D8406
Product Name :
Monoclonal Anti-Dinitrophenyl antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
SPE-7
Applications/Description :
Suitable for indirect ELISA: 0.05-0.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody.
Isotype :
IgE
Concentration :
~1 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA