product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-D 1 Dopamine Receptor antibody produced in rat
catalog :
D2944
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
1-1-F11 s.E6
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section, proximity ligation assay, western blot knockout validation, immunohistochemistry knockout validation
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; rat; 1:500; loading ...; fig 2c
  • western blot; rat; 1:1000; loading ...; fig 1a
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 2a
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 2b
  • immunohistochemistry - frozen section; human; 1:500; loading ...; fig 2d
  • western blot; human; 1:1000; loading ...; fig 1a
Stojanovic T, Orlova M, Sialana F, Höger H, Stuchlík S, Milenkovic I, et al. Validation of dopamine receptor DRD1 and DRD2 antibodies using receptor deficient mice. Amino Acids. 2017;49:1101-1109 pubmed publisher
  • immunohistochemistry knockout validation; mouse; 1:200-1:500; fig 5
  • proximity ligation assay; mouse; fig 6
  • proximity ligation assay; human; fig 6
Frederick A, Yano H, Trifilieff P, Vishwasrao H, Biezonski D, Mészáros J, et al. Evidence against dopamine D1/D2 receptor heteromers. Mol Psychiatry. 2015;20:1373-85 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 4a
Eom T, Han S, Kim J, Blundon J, Wang Y, Yu J, et al. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun. 2020;11:912 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:250
  • immunocytochemistry; mouse; 1:200
Biezonski D, Trifilieff P, Meszaros J, Javitch J, Kellendonk C. Evidence for limited D1 and D2 receptor coexpression and colocalization within the dorsal striatum of the neonatal mouse. J Comp Neurol. 2015;523:1175-89 pubmed publisher
Brodovskaya A, Shiono S, Kapur J. Activation of the basal ganglia and indirect pathway neurons during frontal lobe seizures. Brain. 2021;: pubmed publisher
Hasbi A, Nguyen T, Rahal H, Manduca J, Miksys S, Tyndale R, et al. Sex difference in dopamine D1-D2 receptor complex expression and signaling affects depression- and anxiety-like behaviors. Biol Sex Differ. 2020;11:8 pubmed publisher
Mustafa R, Kreiner G, Kaminska K, Wood A, Kirsch J, Tucker K, et al. Targeted Depletion of Primary Cilia in Dopaminoceptive Neurons in a Preclinical Mouse Model of Huntington's Disease. Front Cell Neurosci. 2019;13:565 pubmed publisher
Grippo R, Tang Q, Zhang Q, Chadwick S, Gao Y, Altherr E, et al. Dopamine Signaling in the Suprachiasmatic Nucleus Enables Weight Gain Associated with Hedonic Feeding. Curr Biol. 2019;: pubmed publisher
Su P, Lai T, Lee F, Abela A, Fletcher P, Liu F. Disruption of SynGAP-dopamine D1 receptor complexes alters actin and microtubule dynamics and impairs GABAergic interneuron migration. Sci Signal. 2019;12: pubmed publisher
Sarker G, Berrens R, von Arx J, Pelczar P, Reik W, Wolfrum C, et al. Transgenerational transmission of hedonic behaviors and metabolic phenotypes induced by maternal overnutrition. Transl Psychiatry. 2018;8:195 pubmed publisher
Jensen K, Sørensen G, Dencker D, Owens W, Rahbek Clemmensen T, Brett Lever M, et al. PICK1-Deficient Mice Exhibit Impaired Response to Cocaine and Dysregulated Dopamine Homeostasis. Eneuro. 2018;5: pubmed publisher
Lavian H, Loewenstern Y, Madar R, Almog M, Bar Gad I, Okun E, et al. Dopamine receptors in the rat entopeduncular nucleus. Brain Struct Funct. 2018;223:2673-2684 pubmed publisher
Hasbi A, Perreault M, Shen M, Fan T, Nguyen T, Alijaniaram M, et al. Activation of Dopamine D1-D2 Receptor Complex Attenuates Cocaine Reward and Reinstatement of Cocaine-Seeking through Inhibition of DARPP-32, ERK, and ΔFosB. Front Pharmacol. 2017;8:924 pubmed publisher
Mor D, Tsika E, Mazzulli J, Gould N, Kim H, Daniels M, et al. Dopamine induces soluble α-synuclein oligomers and nigrostriatal degeneration. Nat Neurosci. 2017;20:1560-1568 pubmed publisher
Grippo R, Purohit A, Zhang Q, Zweifel L, Guler A. Direct Midbrain Dopamine Input to the Suprachiasmatic Nucleus Accelerates Circadian Entrainment. Curr Biol. 2017;27:2465-2475.e3 pubmed publisher
Rassu M, Del Giudice M, Sanna S, Taymans J, Morari M, Brugnoli A, et al. Role of LRRK2 in the regulation of dopamine receptor trafficking. PLoS ONE. 2017;12:e0179082 pubmed publisher
Naneix F, Tantot F, Glangetas C, Kaufling J, Janthakhin Y, Boitard C, et al. Impact of Early Consumption of High-Fat Diet on the Mesolimbic Dopaminergic System. Eneuro. 2017;4: pubmed publisher
Bastide M, Glangetas C, Doudnikoff E, Li Q, Bourdenx M, Fernagut P, et al. Involvement of the bed nucleus of the stria terminalis in L-Dopa induced dyskinesia. Sci Rep. 2017;7:2348 pubmed publisher
Rico A, Dopeso Reyes I, Martínez Pinilla E, Sucunza D, Pignataro D, Roda E, et al. Neurochemical evidence supporting dopamine D1-D2 receptor heteromers in the striatum of the long-tailed macaque: changes following dopaminergic manipulation. Brain Struct Funct. 2017;222:1767-1784 pubmed publisher
Runegaard A, Jensen K, Fitzpatrick C, Dencker D, Weikop P, Gether U, et al. Preserved dopaminergic homeostasis and dopamine-related behaviour in hemizygous TH-Cre mice. Eur J Neurosci. 2017;45:121-128 pubmed publisher
Morigaki R, Goto S. Postsynaptic Density Protein 95 in the Striosome and Matrix Compartments of the Human Neostriatum. Front Neuroanat. 2015;9:154 pubmed publisher
Rivera H, Kievit P, Kirigiti M, Bauman L, Baquero K, Blundell P, et al. Maternal high-fat diet and obesity impact palatable food intake and dopamine signaling in nonhuman primate offspring. Obesity (Silver Spring). 2015;23:2157-64 pubmed publisher
Tobón K, Catuzzi J, Cote S, Sonaike A, Kuzhikandathil E. Post-transcriptional regulation of dopamine D1 receptor expression in caudate-putamen of cocaine-sensitized mice. Eur J Neurosci. 2015;42:1849-57 pubmed publisher
Goto S, Morigaki R, Okita S, Nagahiro S, Kaji R. Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains. Front Neuroanat. 2015;9:22 pubmed publisher
Romero Fernandez W, Borroto Escuela D, Vargas Barroso V, Narváez M, Di Palma M, Agnati L, et al. Dopamine D1 and D2 receptor immunoreactivities in the arcuate-median eminence complex and their link to the tubero-infundibular dopamine neurons. Eur J Histochem. 2014;58:2400 pubmed publisher
Cahill E, Pascoli V, Trifilieff P, Savoldi D, Kappes V, Luscher C, et al. D1R/GluN1 complexes in the striatum integrate dopamine and glutamate signalling to control synaptic plasticity and cocaine-induced responses. Mol Psychiatry. 2014;19:1295-304 pubmed publisher
Simpson E, Winiger V, Biezonski D, Haq I, Kandel E, Kellendonk C. Selective overexpression of dopamine D3 receptors in the striatum disrupts motivation but not cognition. Biol Psychiatry. 2014;76:823-31 pubmed publisher
Mizuta K, Zhang Y, Xu D, Mizuta F, D Ovidio F, Masaki E, et al. The dopamine D1 receptor is expressed and facilitates relaxation in airway smooth muscle. Respir Res. 2013;14:89 pubmed publisher
Ehrlich A, Furuyashiki T, Kitaoka S, Kakizuka A, Narumiya S. Prostaglandin E receptor EP1 forms a complex with dopamine D1 receptor and directs D1-induced cAMP production to adenylyl cyclase 7 through mobilizing G(??) subunits in human embryonic kidney 293T cells. Mol Pharmacol. 2013;84:476-86 pubmed publisher
Hacohen Y, Wright S, Waters P, Agrawal S, Carr L, Cross H, et al. Paediatric autoimmune encephalopathies: clinical features, laboratory investigations and outcomes in patients with or without antibodies to known central nervous system autoantigens. J Neurol Neurosurg Psychiatry. 2013;84:748-55 pubmed publisher
Gildea J, Wang X, Shah N, Tran H, Spinosa M, Van Sciver R, et al. Dopamine and angiotensin type 2 receptors cooperatively inhibit sodium transport in human renal proximal tubule cells. Hypertension. 2012;60:396-403 pubmed publisher
Schlenker E, Schultz H. Hypothyroidism attenuates SCH 23390-mediated depression of breathing and decreases D1 receptor expression in carotid bodies, PVN and striatum of hamsters. Brain Res. 2011;1401:40-51 pubmed publisher
Fiorentini C, Gardoni F, Spano P, Di Luca M, Missale C. Regulation of dopamine D1 receptor trafficking and desensitization by oligomerization with glutamate N-methyl-D-aspartate receptors. J Biol Chem. 2003;278:20196-202 pubmed
Levey A, Hersch S, Rye D, Sunahara R, Niznik H, Kitt C, et al. Localization of D1 and D2 dopamine receptors in brain with subtype-specific antibodies. Proc Natl Acad Sci U S A. 1993;90:8861-5 pubmed
Smiley J, Levey A, Ciliax B, Goldman Rakic P. D1 dopamine receptor immunoreactivity in human and monkey cerebral cortex: predominant and extrasynaptic localization in dendritic spines. Proc Natl Acad Sci U S A. 1994;91:5720-4 pubmed
Hersch S, Ciliax B, Gutekunst C, Rees H, Heilman C, Yung K, et al. Electron microscopic analysis of D1 and D2 dopamine receptor proteins in the dorsal striatum and their synaptic relationships with motor corticostriatal afferents. J Neurosci. 1995;15:5222-37 pubmed
Yung K, Bolam J, Smith A, Hersch S, Ciliax B, Levey A. Immunocytochemical localization of D1 and D2 dopamine receptors in the basal ganglia of the rat: light and electron microscopy. Neuroscience. 1995;65:709-30 pubmed
Zhou Q, Grandy D, Thambi L, Kushner J, Van Tol H, Cone R, et al. Cloning and expression of human and rat D1 dopamine receptors. Nature. 1990;347:76-80 pubmed
product information
Product No. :
D2944
Product Name :
Monoclonal Anti-D 1 Dopamine Receptor antibody produced in rat
Host :
rat
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
1-1-F11 s.E6
Applications/Description :
Suitable for immunocytochemistry: 5-10 μg/mL using D 1 dopamine receptor transfected HEK-293T
Species Reactivity :
monkey, human, rat
Isotype :
IgG2a
Concentration :
~1 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA