Thioredoxin Reductase Assay Kit | MilliporeSigma CS0170 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MilliporeSigma

other brands :

FLUKA, Sigma-Aldrich, Roche Applied Science

product type :

chemical

product name :

Thioredoxin Reductase Assay Kit

catalog :

CS0170

product information

name :

Thioredoxin Reductase Assay Kit

cat no :

CS0170

brand :

SIGMA

prod type :

Chemical

name suffix :

kit sufficient for 100 assays (1 mL)

storage temp :

-20C

storage :

-20C

shipped in :

dry ice

general description :

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5 -dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm. DTNB + NADPH + H+ 2 TNB + NADP+

features and benefits :

. An easy and simple method. The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase . The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase. The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

application :

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).