This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
chemical
product name :
SW48 Cells HIF1A -/-/-
catalog :
CLLS1098
product information
name :
SW48 Cells HIF1A -/-/-
cat no :
CLLS1098
brand :
SIGMA
prod type :
Chemical
storage temp :
-196C
storage :
-196C; -196C
general description :
SW48 Cells HIF1A -/-/- are colorectal adenocarinoma, epithelial cells, from a human adult female, with a ZFN knock out modification.This product corresponds to ATCC Cat. No. CCL-231.
syns :
CLL1008
features and benefits :
These SW48 cells are adherent, with a doubling time of approx. 35 hours.Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2
application :
Media Renenewal changes one to two times per week.Rapidly thaw vial by gentle agitation in 37C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37C in a 5% CO2 atmosphere.Subculture ratio: approx. 1:2 to 1:6.The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Cat. No. S8636, to a final concentration of 1 mM.Cell freezing medium-DMSO 1X, Cat. No. C6164.
company information
MilliporeSigma
questions and comments