This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
chemical
product name :
MCF10A CELLS RICTOR (-/-)
catalog :
CLLS1053
product information
name :
MCF10A CELLS RICTOR (-/-)
cat no :
CLLS1053
brand :
SIGMA
prod type :
Chemical
storage temp :
-196C
storage :
-196C
general description :
This cell line corresponds to ATCC Catalog No. CRL-10317.; MCF10A CELLS RICTOR (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
features and benefits :
Zinc finger nuclease (ZFN) knockout on chromosome 5q13.1; These MCF10A cells are adherent with a doubling time of approx. 20 hours
application :
Rapidly thaw vial by gentle agitation in 37C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37; Cell freezing medium-DMSO 1X, Cat. No. C6164; The base medium for this cell line is Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.; Media renewal changes two to three times per week.
company information
MilliporeSigma
questions and comments