MCF10A CELLS CDH1 (-/-) | MilliporeSigma CLLS1042 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MilliporeSigma

other brands :

FLUKA, Sigma-Aldrich, Roche Applied Science

product type :

chemical

product name :

MCF10A CELLS CDH1 (-/-)

catalog :

CLLS1042

citations: 1

Reference
Chen A, Beetham H, Black M, Priya R, Telford B, Guest J, et al. E-cadherin loss alters cytoskeletal organization and adhesion in non-malignant breast cells but is insufficient to induce an epithelial-mesenchymal transition. BMC Cancer. 2014;14:552 pubmed publisher

product information

name :

MCF10A CELLS CDH1 (-/-)

cat no :

CLLS1042

brand :

SIGMA

prod type :

Chemical

storage temp :

-196C

storage :

-196C

general description :

MCF10A CELLS CDH1 (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.; This cell line corresponds to ATCC Catalog No. CRL-10317.

features and benefits :

These MCF10A cells are adherent with a doubling time of approx. 48 hours; Zinc finger nuclease (ZFN) knockout on chromosome 16q22.1

application :

Rapidly thaw vial by gentle agitation in 37C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37; Cell freezing medium-DMSO 1X, Cat. No. C6164; The base medium for this cell line is Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.; Media renewal changes two to three times per week.